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Loss of liver kinase B1 causes planar polarity defects in cochlear hair cells in mice

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Frontiers of Medicine 2016年第4期10卷 481-489页

作者： Yuqin Men Aizhen Zhang Liwen Zhang Yecheng Jin Zhishuo Wang Jing Zhao Xiaolin Yu Jian Zhang jiangang gao Sehool of Life Science and Key Laboratory of the Ministry of Education for Experimental Teratology Shandong University Jinan 250100 China jinan First People's Hospital Jinan 250011 China

The tumor suppressor gene liver kinase B1 （LKB1）, also called STKll, encodes a serine/threonine kinase. LKB1 plays crucial roles in cell differentiation, proliferation, and polarity. In this study, LKB1 conditional knockout mice （LKB1pax2 CKO mice） were generated using Pax2-Cre mice to investigate the function of LKB1 in inner ear hair cells during early embryonic period. LKB1Pax2 CKO mice died perinatally. Immunofluorescence and scanning electron microscopy revealed that stereociliary bundles in LKB1Pax2 CKO mice were clustered and misoriented, respectively. Moreover, ectopic distribution of kinocilium bundles resulting from abnormal migration of kinocilium was observed in the mutant mice. The orientation of stereociliary bundles and the migration of kinocilia are critical indicators of planar cell polarity （PCP） of hair cells. LKB1 deficiency in LKB1Pax2 CKO mice thus disrupted hair cell planar polarity during embryonic development. Our results suggest that LKB1 is required in PCP formation in cochlear hair cells in mice.

关键词： LKB1 stereociliary bundles kinocilium planar cell polarity hearing mice

Transcriptomic and metabolomic analysis provides insights into lignin biosynthesis and accumulation and differences in lodging resistance in hybrid wheat

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Journal of Integrative Agriculture 2024年第4期23卷 1105-1117页

作者： Weibing Yang Shengquan Zhang Qiling Hou jiangang gao Hanxia Wang Xianchao Chen Xiangzheng Liao Fengting Zhang Changping Zhao Zhilie Qin Institute of Hybrid Wheat Beijing Academy of Agriculture and Forestry SciencesBeijing 100097China Beijing Municipal Key Laboratory of Molecular Genetics of Hybrid Wheat Beijing 100097China

The use of hybrid wheat is one way to improve the yield in the ***,greater plant heights increase lodging risk to some *** this study,two hybrid combinations with differences in lodging resistance were used to analyze the stem-related traits during the filling stage,and to investigate the mechanism of the difference in lodging resistance by analyzing lignin synthesis of the basal second internode(BSI).The stem-related traits such as the breaking strength,stem pole substantial degree(SPSD),and rind penetration strength(RPS),as well as the lignin content of the lodging-resistant combination(LRC),were significantly higher than those of the lodgingsensitive combination(LSC).The phenylpropanoid biosynthesis pathway was significantly and simultaneously enriched according to the transcriptomics and metabolomics analysis at the later filling stage.A total of 35 critical regulatory genes involved in the phenylpropanoid pathway were ***,42%of the identified genes were significantly and differentially expressed at the later grain-filling stage between the two combinations,among which more than 80%were strongly up-regulated at that stage in the LRC compared with *** the contrary,the LRC displayed lower contents of lignin intermediate metabolites than the *** results suggested that the key to the lodging resistance formation of LRC is largely the higher lignin synthesis at the later grain-filling ***,breeding strategies for synergistically improving plant height and lodging resistance of hybrid wheat were put forward by comparing the LRC with the conventional wheat applied in large areas.

关键词： gene expression lignin synthesis lodging-resistance hybrid wheat

Sunway supercomputer architecture towards exascale computing: analysis and practice

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Science China(Information Sciences) 2021年第4期64卷 177-197页

作者： jiangang gao Fang ZHENG Fengbin QI Yajun DING Hongliang LI Hongsheng LU Wangquan HE Hongmei WEI Lifeng JIN Xin LIU Daoyong GONG Fei WANG Yan ZHENG Honghui SUN Zhou ZHOU Yong LIU Hongtao YOU National Research Center of Parallel Computer Engineering and Technology

In recent years, the improvements of system performance and energy efficiency for supercomputers have faced increasing challenges, which create more intensive demands on the architecture design for realizing exascale computing. This paper first analyzes the main requirements of exascale computing on the aspects of the parallel computing application and supercomputing center operation. Afterwards, a mapping scheme of "demands-challenges-architecture" is proposed. Then, the major challenges of exascale supercomputer, such as scalability, power consumption, data movement, programming and availability, are thoroughly analyzed, and the corresponding appropriate solutions are proposed. Moreover, this paper proposes the Sunway computer architecture towards exascale computing in which the many-core processor, network chipset and software system are all domestically-designed. The technology roadmap of Sunway supercomputer will hold the comprehensive design methods for the architecture, including the processor, interconnect network,assembly structure, power supply, cooling system, system software, parallel algorithm and application support, promising great advances for exascale supercomputing.

关键词： supercomputer exascale Sunway scalability power consumption data movement programming availability

Tprn is essential for the integrity of stereociliary rootlet in cochlear hair cells in mice

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Frontiers of Medicine 2019年第6期13卷 690-704页

作者： Yuqin Men Xiujuan Li Hailong Tu Aizhen Zhang Xiaolong Fu Zhishuo Wang Yecheng Jin Congzhe Hou Tingting Zhang Sen Zhang Yichen Zhou Boqin Li Jianfeng Li Xiaoyang Sun Haibo Wang jiangang gao School of Life Science Shandong UniversityJinan 250100China Rizhao Polytechnic Rizhao 276826China The Second Hospital of Shandong University Jinan 250033China Electron Microscopy Laboratory Shandong Institute of OtolaryngologyJinan 250022China Laboratory of Electron Microscopy Jinan WEI-YA Biotech CompanyJinan 250100China Department of Otolaryngology-Head and Neck Surgery Provincial Hospital Affiliated to Shandong UniversityJinan 250021China

Tprn encodes the taperin protein,which is concentrated in the tapered region of hair cell stereocilia in the inner *** humans,TPRN mutations cause autosomal recessive nonsyndromic deafness(DFNB79)by an unknown *** determine the role of Tprn in hearing,we generated Tprn-null mice by clustered regularly interspaced short palindromic repeat/Cas9 genome-editing technology from a CBA/CaJ *** observed significant hearing loss and progressive degeneration of stereocilia in the outer hair cells of Tprn-null mice starting from postnatal day *** electron microscopy images of stereociliary bundles in the mutant mice showed some stereociliary rootlets with curved *** central cores of the stereociliary rootlets possessed hollow structures with surrounding loose peripheral dense ***,a protein expressed at stereocilia tapering,was abnormally dispersed along the stereocilia shafts in Tprn-null *** expression levels of radixin andβ-actin significantly *** propose that Tprn is critical to the retention of the integrity of the stereociliary *** of Tprn in Tprn-null mice caused the disruption of the stereociliary rootlet,which resulted in damage to stereociliary bundles and hearing *** generated Tprn-null mice are ideal models of human hereditary deafness DFNB79.

关键词： TPRN stereocilia stereociliary rootlet actin filament CRISPR/Cas9 hearing

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谁是专利蟑螂:基于美国非专利实施体的研究

情报杂志 2020年第11期39卷 61-67页

作者：高建刚聊城大学商学院聊城252000

[目的/意义]专利蟑螂(Patent Troll)属于非专利实施体(Non-Practicing Entities,NPEs)的一种。随时间推移,中国将是专利蟑螂的理想之地,这将对中国创新活动和知识产权有效保护会带来较大不利影响,正确识别专利蟑螂具有重要意义。[方法/... 详细信息

[目的/意义]专利蟑螂(Patent Troll)属于非专利实施体(Non-Practicing Entities,NPEs)的一种。随时间推移,中国将是专利蟑螂的理想之地,这将对中国创新活动和知识产权有效保护会带来较大不利影响,正确识别专利蟑螂具有重要意义。[方法/过程]为厘清这一问题,该文运用美国专利诉讼资料,将原告专利所有人分为七类,运用Logistic回归模型,实证考察原告类型、专利质量以及MLPs对诉讼和解率的影响来回应这一问题。[结果/结论]研究发现:不同类型NPEs的专利质量及其对和解率的影响并不相同:大学、前新创公司、专利授权公司及技术研发公司发起的诉讼案中,专利质量提升对和解率有正向显著影响;个体、大型专利整合者的专利质量对和解率影响则相反;一般而言,MLPs占诉讼案专利的比例越高,和解率越低;不过在个体发明者及专利授权公司发起的诉讼案中,则有和解率较高的趋势。由此推断个体发明者及专利授权公司等NPEs类别更符合专利蟑螂的特征界定。最后给出了相关政策及建议。

关键词：专利蟑螂专利质量多次发起诉讼专利诉讼和解率

中国新能源汽车产业创新系统的发展与失灵研究

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情报杂志 2019年第12期38卷 77-85,91页

作者：高建刚聊城大学商学院

[目的/意义]新能源汽车是中国重要的战略性新兴产业,在引领产业转型升级,推动绿色发展方面具有重要现实意义,目前的研究缺少系统性的理论分析框架,未能较为全面地观察新能源汽车产业的创新系统如何演化,也未能深入探讨该产业在发展过程... 详细信息

[目的/意义]新能源汽车是中国重要的战略性新兴产业,在引领产业转型升级,推动绿色发展方面具有重要现实意义,目前的研究缺少系统性的理论分析框架,未能较为全面地观察新能源汽车产业的创新系统如何演化,也未能深入探讨该产业在发展过程中所面临的系统性障碍。[方法/过程]运用产业创新系统(SSI)的理论框架,综合使用专利检索与分析、文献搜集与专家访谈相结合的研究方法,从知识与技术、行动者及其网络、基础设施和制度等四个维度考察了中国新能源汽车产业创新系统的发展以及遭遇的障碍即系统失灵。[结果/结论]研究发现,尽管中国新能源汽车产业在上述四个维度均获得了较大发展,但同时存在不同程度的系统失灵现象。其中,基础设施和制度失灵最为严重,其次是知识与技术失灵,再次是行动者及其网络失灵。此外,还存在行动者之间因共同研发和技术交流不足或技术方向不一致而导致的连结失灵,文章最后给出了政策建议与措施。

关键词：新能源汽车产业创新系统知识与技术失灵行动者与网络失灵制度失灵

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我国医药生物产业的研发弹性和技术效率研究

科技管理研究 2014年第19期34卷 149-153页

作者：高建刚聊城大学商学院山东聊城252059

以沪深两市2009-2012年医药生物类上市公司为样本,实证分析医药生物类产业的研发弹性和技术效率。研究发现:无论采用可变规模报酬还是固定规模报酬模型,医药生物类企业的研发支出对企业产值均有显著正向影响,研发弹性在0.10-0.20左右。... 详细信息

以沪深两市2009-2012年医药生物类上市公司为样本,实证分析医药生物类产业的研发弹性和技术效率。研究发现:无论采用可变规模报酬还是固定规模报酬模型,医药生物类企业的研发支出对企业产值均有显著正向影响,研发弹性在0.10-0.20左右。然后运用随机前沿分析方法考察企业的技术效率和影响因素,发现医药生物类企业的平均技术效率在0.75左右,还有很大的提升空间。公司成立年限越长、国有股权比例越高,越有助于提升企业效率,处于我国中部地区以及资产负债率较高的公司,效率会受到负面影响。

关键词：医药生物产业研发弹性技术效率随机前沿方法

再生能源生产和消费中的外部性与最优政策——以生物质能为例

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北京理工大学学报（社会科学版） 2016年第1期18卷 38-44页

作者：高建刚聊城大学商学院山东聊城252059

再生能源具有较好的环保效果但是却有较高的成本,实践中政府普遍依据再生能源产业的总产量进行补贴。然而,再生能源的消费虽具有环境友善性,但其生产过程,一般要消耗传统能源,仍然会对环境产生一定污染,仅有补贴未免失之偏颇,且对总产... 详细信息

再生能源具有较好的环保效果但是却有较高的成本,实践中政府普遍依据再生能源产业的总产量进行补贴。然而,再生能源的消费虽具有环境友善性,但其生产过程,一般要消耗传统能源,仍然会对环境产生一定污染,仅有补贴未免失之偏颇,且对总产量补贴也有不当之处。若将再生能源生产过程中的环境污染纳入考虑时,政府应当如何应对?研究发现:第一,无论厂商是否具有污染治理技术,也无论市场结构如何,最优污染治理税率均应等于污染边际损害,即最优污染治理税为庇古税。第二,在完全竞争市场结构下,最优补贴率等于再生能源的边际收益,即最优补贴率为庇古补贴;在寡占市场结构下,最优补贴率应大于再生能源的边际收益,且厂商市场势力越大,最优补贴率越高。

关键词：再生能源污染排放税污染补贴边际污染损害

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金融制度创新的演化博弈分析

广东金融学院学报 2009年第4期24卷 21-31页

作者：高建刚聊城大学商学院山东聊城252000

从分业经营走向混业经营是一项重要的金融制度创新。基于供求的主流经济学分析难以面对金融创新的复杂性、自发性、创新过程的不可逆性以及环境的选择性,因而不能有效地对金融创新做出合理的解释。运用演化博弈理论对金融业从分业经营... 详细信息

从分业经营走向混业经营是一项重要的金融制度创新。基于供求的主流经济学分析难以面对金融创新的复杂性、自发性、创新过程的不可逆性以及环境的选择性,因而不能有效地对金融创新做出合理的解释。运用演化博弈理论对金融业从分业经营到混业经营的金融制度创新过程研究发现,金融制度创新是金融体系内部各方参与者动态博弈的过程,是经济体系自生自发的过程,具有环境选择性。

关键词：跨业经营分业经营演化博弈金融制度创新