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Influence of bone morphogenetic protein type IA receptor conditional knockout in lens on expression of bone morphogenetic protein 4 in lens

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International Journal of Ophthalmology(English edition) 2015年第1期8卷 57-60页

作者： Qi zhao jiang-yue zhao Jin-Song Zhang Department of Ophthalmology the Second Affiliated Hospital of Dalian Medical University Department of Ophthalmology the Fourth Affiliated Hospital of China Medical University

AIM: To investigate the influence of bone morphogenetic protein type IA receptor [BMPR-IA(ALK3)] conditional knockout in lens on expression of bone morphogenetic protein 4(BMP4) in lens during the development of the vertebrate ***: Cre-positive mice were mated with Crenegative mice to generate 50% Cre-positive(conditional knockout, CKO) 4 embryos, 8 eyes and 50% Cre-negative offspring(wild type, WT) 4 embryos, 8 eyes. The embryos were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned to a thickness of 4 μ*** of paraffin wax and dehydrating for sections,and then the procedure of in situ hybridization was processed, BMP4 MK1784-m(BOSTER) was used, and observed the expression of BMP4 in the lens in experimental group and control group. We selected SPSS11.0 software for statistical analysis, P<0.05 showed that the difference was statistically significant.· RESULTS: Four embryos of each genotype were examined, totally we had 8 embryos, 16 eyes. We got the uniform outcomes in all the embryos. We found ALK3 was required during lens growing, but was not essential for the formation of lens. We observed that the expression of BMP4 in the lens was significantly reduced in all 8 ALK3 CKO lens, BMP4 expression was normal in all the 8 WT lens, P <0.01. This phenomenon became increasingly visible in accordance with embryo development. The most apparent alteration was present at stage ***: ALK3 is essential for lens growth. The influence of ALK3 on the expression of BMP4 is present during the development of mice lens.

关键词： bone morphogenetic protein type IA receptor bone morphogenetic protein 4 lens

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Regulation of Eaf2 in mouse lens cells apoptosis induced by ultraviolet radiation

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International Journal of Ophthalmology(English edition) 2012年第5期5卷 570-575页

作者： Fan Xiao Jin-Song Zhang jiang-yue zhao and Di Wu Department of Ophthalmology the Fourth Affiliated Hospital of China Medical University Shenyang 110005 Liaoning Province China

AIM: To investigate the regulation of Eaf2 protein in mouse lens cells apoptosis induced by ultraviolet (UV) radiation. METHODS: An eye of Eaf2 gene knockout mice or normal control mice was exposed to UV radiation, and the other one was non-exposed. All of lenses were analyzed by TUNEL and caspase 3 activity assays to determine the difference of the apoptosis induced by UV radiation. In addition, exposed and non-exposed lenses were analyzed by quantified p53 expression and real-time reverse transcription-polymerase chain reaction (RT-PCR) of Bax, Bid, Apaf-1, Puma and Noxa, to compare Eaf2 gene knockout mice and normal control mice. RESULTS: UV radiation caused apoptosis of lens cells in normal control mice and Eaf2 knockout mice. Activity of caspase 3 was significantly higher in normal control mice than Eaf2 knockout mice. Expression of p53 protein was significantly higher in lenses exposed to UV radiation than nonexposed lenses, but was similar between Eaf2 gene knockout mice and normal control mice in the same UV condition. After exposing to UV radiation, the analysis of real-time RT-PCR demonstrated that mRNA levels of Puma and Noxa were significantly higher in lenses of normal control mice than Eaf2 gene knockout mice, and that mRNA levels of Bax, Bid and Apaf-1 were not significantly different between gene knockout mice and normal control mice. CONCLUSION: Eaf2 increases lens cells apoptosis induced by ultraviolet radiation. And Eaf2 up-regulates expression of the Puma and the Noxa to act on lens cells apoptosis after UV radiation.

关键词： Eaf2 p53 apoptosis Noxa Puma ultraviolet rays

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Role of Smad4 from ocular surface ectoderm in retinal vasculature development

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International Journal of Ophthalmology(English edition) 2020年第2期13卷 231-238页

作者： Jing Li Jin-Song Zhang jiang-yue zhao Guo-Ge Han Tianjin Eye Hospital Tianjin Eye InstituteTianjin Key Lab of Ophthalmology and Visual ScienceNankai UniversityTianjin 300000China Shenyang Aier Excellence Eye Hospital Shenyang 110000Liaoning ProvinceChina Department of Ophthalmology the Fourth Affiliated Hospital of China Medical UniversityEye Hospital of China Medical UniversityKey Lens Research LaboratoryShenyang 110000Liaoning ProvinceChina

●AIM:To investigate how signals from lens regulate retinal vascular development and neovascularization.●METHODS:Le-Cre transgenic mouse line was employed to inactivate Smad4 in the surface ectoderm *** histological and whole-mount retina staining were employed to reveal morphological changes of retinal vasculature in Smad4 defective *** microarray and subsequent analyses were conducted to investigate the molecular mechanism underlying the vascular *** polymerase chain reaction(qPCR)was carried out to verify the microarrays results.●RESULTS:We found that inactivation of Smad4 specifically on surface ectoderm leads to a variety of retinal vasculature *** analyses and qPCR revealed that Sema3 c,Sema3 e,Nrp1,Tie1,Sox7,Sox17,and Sox18 are significantly affected in the knockout retinas at different developmental stages,suggesting that ocular surface ectoderm-derived Smad4 can signal to the retina and regulates various angiogenic signaling in the retina.●CONCLUSION:Our data suggest that the cross-talk between ocular surface ectoderm and retina is important for retinal vasculature development,and Smad4 regulates various signaling associated with sprouting angiogenesis,vascular remodeling and maturation in the retina of mice.

关键词： Smad4 retinal vasculature surface ectoderm

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Acute lens opacity induced by different kinds of anesthetic drugs in mice

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International Journal of Ophthalmology(English edition) 2019年第6期12卷 904-908页

作者： Xiao-Tong Li Yu Qin jiang-yue zhao Jin-Song Zhang Department of Ophthalmology the Fourth Affiliated Hospital of China Medical UniversityEye Hospital of China Medical UniversityKey Lens Research Laboratory of Liaoning ProvinceShenyang 110005Liaoning ProvinceChina Aier Eye Hospital Shenyang 110000Liaoning ProvinceChina

AIM: To study whether specific anesthetic drugs or tear layer evaporation was primarily responsible for the acute cataract and what the change of lens structure is in anesthetized ***: Five groups were set up in the experiment: Group A(topicamide and phenylephrine mixed eye drop+ chloral hydrate), Group B(tropicamide and phenylephrine mixed eye drop+sevoflurane), Group C(tropicamide and phenylephrine mixed eye drop), Group D(topicamide and phenylephrine mixed eye drop+chloral hydrate, carbomer eye drop in the right eyes), and Group E(tropicamide and phenylephrine mixed eye drop+sevoflurane, carbomer eye drop in the right eyes). A simple classification system was used to assess the severity of lens opacity. And a numerical value from 0 to 3 to each grade was assigned for the cataract index calculation and data analysis. The gross appearance and time course of development of lens opacity were assessed. Hematoxylin and eosin staining was used to observe the lens structure changes in the reversible ***: Tropicamide did not induce lens opacification in mice. Lens opacity caused by inhaled sevoflurane was similar to injected cholral hydrate. Both inhaled-anestheticinduced lens opacity and injected-anesthetic-induced lens opacity could be prevented by carbomer eye drop. In the severe opacity lens, a wide range of lens fiber cell structure had disordered. The fiber cells became uneven ***: The acute reversible lens opacity can unilaterally develop or be induced by a local cause. The structure of lens fiber cells changed in the lens opacity which may influence the permanent connection of the lens fiber cells. This study was not only of practical significance to help maintain lens transparency for eye research, but also of the deeper consideration about the reversible lens opacification phenomenon.

关键词： lens opacity anesthetic drugs tear film mice

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Distance vision after bilateral implantation of AcrySof toric intraocular lenses:a randomized,controlled,prospective trial

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International Journal of Ophthalmology(English edition) 2011年第2期4卷 175-178页

作者： Jin-Song Zhang jiang-yue zhao Qi Sun and Li-Wei Ma Eye Hospital of China Medical University Department of Ophthalmology the Fourth Affiliated Hospital of China Medical University Provincial Key Laboratory of Lens Research Liaoning China

·AIM:To evaluate the distance vision of Chinese patients with cataracts and corneal astigmatism after implantation of bilateral AcrySof toric intraocular lens (IOL) versus bilateral AcrySof spherical IOL.·METHODS:This study randomized 60 patients into equal groups to receive toric IOL or spherical *** powers targeting emmetropia were selected for 93% of toric IOL patients and for 90% of spherical IOL *** included monocular and binocular distance vision,with and without best *** also completed surveys about their distance vision.·RESULTS:Preoperatively,the two study groups were similar in age,in distance visual acuity,and in the magnitude of corneal *** 6 months postoperative,binocular uncorrected distance vision was 0.06?à0.14 logMAR in the AcrySof toric IOL group,significantly better than the 0.14?à0.11 logMAR in the spherical IOL group (P<0.05).For eyes with emmetropia as a target,the equivalent of 20/20 uncorrected vision was more likely (P<0.001) in the toric IOL group (36% of eyes) than in the spherical IOL group (4% of eyes).No patients in the emmetropia/toric IOL group used distance glasses,as compared to 52% of patients in the emmetropia/spherical IOL *** patients were satisfied or highly *** of distance vision was rated higher by toric IOL patients than by spherical IOL patients (P<0.05).·CONCLUSION:Bilateral AcrySof toric IOL is superior to bilateral spherical IOL in providing uncorrected distance vision to cataract patients with corneal astigmatism.·

关键词： astigmatism cataract extraction lenses intraocular pseudophakia

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Trends in research related to high myopia from 2010 to 2019:a bibliometric and knowledge mapping analysis

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International Journal of Ophthalmology(English edition) 2021年第4期14卷 589-599页

作者： Xiao-Dan Zhang Chun-Xia Wang Hong-Hu jiang Shuo—Lan Jing jiang-yue zhao Zi-Yan Yu Department of Ophthalmology the Fourth Affiliated Hospital of China Medical UniversityEye Hospital of China Medical UniversityKey Lens Research Laboratory of Liaoning ProvinceShenyang 110005Liaoning ProvinceChina China Medical University Shenyang 110122Liaoning ProvinceChina

AIM:To evaluate the global trends in and explore hotspots of high myopia(HM)***:This bibliometric analysis was used to reveal the publication trends in HM research field based on the Web of Science Core Collection(WoSCC).VOSviewer version 1.6.13 software was used to analyze the data and construct a knowledge map including the yearly publication number,journals,countries,international collaborations,authors,research hotspots,and intellectual base in ***:The search engine found 3544 peer-reviewed publications on HM between 2010 and 2019,and the yearly research output substantially elevated over the past *** is the top publishing country,and Sun Yatsen University was the most active academic *** JB is the top publishing scientist,and Investigative Ophthalmology and Visual Science(IOVS)was the most productive *** highest cited references mainly focused on epidemiology and *** keywords formed 6 clusters:1)refractive surgery;2)etiology and clinical characteristics;3)the mechanism of eye growth;4)management for myopic maculopathy;5)vitrectomy surgical treatment;6)myopia-associated glaucoma-like optic ***:The evaluation of development trends based on the data extracted from WoSCC can provide valuable information and guidance for ophthalmologists and public health researchers to improve management procedures in HM field.

关键词： high myopia pathological myopia bibliometric analysis VOSviewer

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Visual function and higher order aberration after implantation of aspheric and spherical multifocal intraocular lenses:a meta-analysis

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International Journal of Ophthalmology(English edition) 2013年第5期6卷 690-695页

作者： Jian-Ping Liu Fan Zhang jiang-yue zhao Li-Wei Ma Jin-Song Zhang Department of Ophthalmology the Fourth Affiliated Hospital of China Medical University Eye Hospital of China Medical University Key Lens Research Laboratory of Liaoning Province

AIMTo assess the visual outcomes of aspheric multifocal intraocular lenses (IOLs) compared with spherical multifocal IOL after cataract surgery.

关键词： aspheric multifocal intraocular lens uncorrected near visual acuity uncorrected distance visual acuity spherical multifocal intraocular lens higher order aberration meta-analysis

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Msx2 plays a critical role in lens epithelium cell cycle control

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International Journal of Ophthalmology(English edition) 2013年第3期6卷 276-279页

作者： jiang-yue zhao Feng-Feng Zhuang Hong-Yan Wang Di Wu Jin-Song Zhang Eye Hospital of China Medical University Department of Ophthalmology the 4~(th) Affiliated Hospital of China Medical University Provincial Key Laboratory of Lens Research Department of Ophthalmology and Doheny Eye Institute Keck School of Medicine University of Southern California Department of General Surgery People's Hospital of Liaoning Province

AIM: To investigate the effects of Msx2 on lens epithelium cell cycle, and evaluate the changes of the proliferation, apoptosis of lens epithelium cells. METHODS: Mice lens epithelium cells were cultured and transfected with pEGFP-Msx2 and control. Msx2-deficient mice (Msx2(-/-)) lens tissue were isolated. Lens tissue and transfected cells were prepared for mRNA extraction using Trizol reagent. CyclinD1 and Prox1 expression were evaluated by real-time RT-PCR. BrdU incorporation and apoptosis rate were investigated by immunofluorescence and flow cytometry analysis. RESULTS: After transfected with pEGFP-Msx2, lens epithelium cells failed to incorporate BrdU and anti - phospho-histone-3 immunofluorescence failed to detect cell nuclei which GFP were positive. Msx2 over expression resulted in increasing apoptosis rate in lens epithelium cells. CyclinD1 and Prox1 expression increased significantly in Msx2 knockout mice by real time RT-PCR quantization and CyclinD1 expression decreased significantly in Msx2overexpressed cell. CONCLUSION: Msx2 has the effect of inhibiting proliferation and differentiation, triggering apoptosis on mice lens epithelium cells.

关键词： Msx2 lens epithelium cell cell cycle

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Anterior segment dysgenesis correlation with epithelial-mesenchymal transition in Smad4 knockout mice

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International Journal of Ophthalmology(English edition) 2016年第7期9卷 943-947页

作者： Jing Li Yu Qin Fang-Kun zhao Di Wu Xue-Fei He Jia Liu jiang-yue zhao Jin-Song Zhang Department of Ophthalmology the Fourth Affiliated Hospital of China Medical University Eye Hospital of China Medical University Key Lens Research Laboratory of Liaoning Province The Shenyang Fourth People's Hospital Shenyang Eye Research Institute Department of Ophthalmology Ningbo No.2 Hospital

AIM： To explore the molecular mechanisms in lens development and the pathogenesis of Peters anomaly in Smad4 defective mice. METHODS： Le-Cre transgenic mouse line was employed to inactivate Smad4 in the surface ectoderm selectively. Pathological techniques were used to reveal the morphological changes of the anterior segment in Smad4 defective eye. Immunohistochemical staining was employed to observe the expression of E-cadherin, N- cadherin and α-SMA in anterior segment of Smad4 defective mice and control mice at embryonic （E） day 16.5. Real-time quantitative polymerase chain reaction （qPCR） was performed to detect the expression of Snail, Zebl, Zeb2 and Twist2 in lens of Smad4 defective mice and control mice at E16.5. RESULTS： Conditional deletion of Smad4 on eye surface ectoderm resulted in corneal dysplasia, iridocorneal angle closure, corneolenticular adhesions and cataract resembling Peters anomaly. Loss of Smad4 function inhibited E-cadherin expression in the lens epithelium cells and corneal epithelium cells in Smad4 defective eye. Expression of N-cadherin was upregulated in corneal epithelium and corneal stroma. Both E-cadherin and N-cadherin were down-regulated at the future trabecular meshwork region in mutant eye. The qPCR results showed that the expression of Twist2 was increased significantly in the mutant lens （P〈0.01）. CONCLUSION： Smad4 is essential to eye development and likely a candidate pathogenic gene to Peters anomaly by regulating epithelial-mesenchymal transition. Twist2 can be regulated by Smad4 and plays an essential role in lens development.

关键词： Peters anomaly anterior segment dysgenesis Smad4 N-cadherin Twist2

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Human lens epithelial cell apoptosis and epithelial to mesenchymal transition in femtosecond laser-assisted cataract surgery

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International Journal of Ophthalmology(English edition) 2018年第3期11卷 401-407页

作者： Wei Sun Jia Liu Jing Li Di Wu Jing Wang Ming-Wu Wang Jin-Song Zhang jiang-yue zhao Department of Ophthalmology the 4th Affiliated Hospital of China Medical University Eye Hospital of China Medical University Provincial Key Laboratory of Lens Research Shenyang 110005 Liaoning Province China Department of Ophthalmology and Vision Science the University of Arizona College of Medicine Tucson AZ 85711-1824 USA

AIM： To evaluate human lens epithelium cell apoptosis and epithelial to mesenchymal transition （EMT） induced by femtosecond laser in femtosecond laser assisted cataract surgery （FLACS）. METHODS： Sixty cataract patients with N2 to N3 stage according to the LOCS III were enrolled in this study and divided into three groups randomly： FLACSl group （cataract surgery by FLACS with LenSx）, FLACS2 group （cataract surgery by FLACS with LensAR） and manual group （cataract surgery by phacoemulsification）. Patients in two FLACS groups performed anterior capsulotomy by LenSx or LensAR laser system. Patients in the manual group were performed continuous curvilinear capsulorrhexis （CCC） manually. The anterior capsules were fixed right after moved out of eye. Hematoxylin-eosine staining, immunofluorescence staining and real-time PCR were performed in order to observe human lens epithelium cells changes after cataract surgery. RESULTS： The capsule cutting edge was shown irregularity and roughness in two FLACS groups and smooth edge in manual capsulotomy by pathologic staining. Irregularities of the cell configuration with partly swollen and destroyed nuclei were observed in two FLACS groups. Femtosecond laser could induce a significantly higher cell apoptosis in human lens epithelium cell than manually performed CCC （P〈0.05）. Lens epithelium cells apoptosis were correlated with femtosecond laser duration according to Pearson correlation analysis. Decreased N-cadherin expression, alpha-SMA and FSP-1 level in two FLACS groups showed the inhibition of cell EMT. CONCLUSION： Femtosecond laser may affect the apoptosis and EMT of lens epithelium cells which are under the peeled central lens capsule.

关键词： femtosecond lasers assisted cataract surgery lens epithelium cell apoptosis epithelial mesenchymal transition

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