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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第2期9卷 148-153页

作者： Zong-zhao YANG jiang-bing shuai Xian-jun DAI Wei-huan FANG Institute of Preventive Veterinary Medicine Zhejiang University Hangzhou 310029 China Hangzhou Husbandry and Veterinary Station Hangzhou 310020 China College of Life Science China Jiliang University Hangzhou 310018 China

Porcine circovirus type 2 (PCV2) is closely related to the postweaning multisystemic wasting syndrome (PMWS). In this study, the pig serum and tissue samples collected from different regions of Hangzhou District in Zhejiang Province of China between 2003 and 2005 were analyzed by enzyme-linked immunosorbent assay (ELISA) for PCV2 antibody and by polymerase chain reaction (PCR) for ORF2 gene. The results show that out of 1250 randomly collected serum samples, 500 sera (40%) were seropositive for PCV2. PCR results demonstrate that Hangzhou PCV2 with more than 50% Chinese PCV2 strains and French PCV2 formed Cluster A. Only one PCV2 from Hangzhou belonged to Cluster B with some other Chinese PCV2 and Netherlands’s isolates. Cluster C consisted of PCV2 isolates from China, US, Canada, UK and Germany. The results indicate that the PCV2 infection was widespread in Hangzhou.

关键词： Porcine circovirus type 2 (PCV2) Seroprevalence ORF2 Phylogenetic analysis

Virulence Phenotyping and Molecular Characterization of a Low-pathogenicity Isolate of Listeria monocytogenes from Cow's Milk

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Acta Biochimica et Biophysica Sinica 2006年第4期38卷 262-270页

作者： Ling-Li jiang Jing-Jing XU Ning CHEN jiang-bing shuai Wei-Huan FANG* Institute of Preventive Veterinary Medicine,Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine,Zhejiang University,Hangzhou 310029,China Institute of Preventive Veterinary Medicine Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine Zhejiang UniversityHangzhou 310029 China

A low-pathogenicity isolate of Listeria monocytogenes from cow’s milk,as screened in mouseand chicken embryonated egg models,was examined for virulence-related phenotypic *** genes (iap,prfA,plcA,hly,mpl,actA,plcB,InlA and InlB) were compared with *** strains 10403S and EGD to elucidate the possible molecular mechanisms of low ***-though *** H4 exhibited similar patterns to strain 10403S in terms of hemolytic activity,invitro growth and invasiveness and even had higher adhesiveness,faster intracellular growth and higherphospholipase activity in vitro,it was substantially less virulent than the strain 10403S in mouse and chickenembryo models (50% lethal dose:10vs.10and 10vs.10,respectively).The genes prfA,plcA andmpl were homologous among *** strains H4,10403S and EGD (>98%).Genes iap,hly,plcB,InlA and InIB of *** 10403S had higher homology to those of strain EGD (>98%) than *** homology of the gene hly between strain 10403S and isolate H4 was 96.9% at the nucleotide level,but 98.7% at the amino acid *** actA gene of isolate H4 had deletions of 105 nucleotides correspondingto 35 amino acid deletions falling Within the proline-rich *** together,this study presents someclues as to reduced virulence to mice and chicken embryos of the isolate H4 probably as a result of deletionmutations of actA.

关键词： Listeria monocytogenes virulence factor virulence-associated gene

Biomaterial‑based mechanical regulation facilitates scarless wound healing with functional skin appendage regeneration

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Military Medical Research 2025年第1期12卷 96-120页

作者： Ying-Ying Li shuai-Fei Ji Xiao-bing Fu Yu-Feng jiang Xiao-Yan Sun Research Center for Tissue Repair and Regeneration Affiliated to the Medical Innovation Research Department Chinese PLA General Hospital and PLA Medical CollegePLA Key Laboratory of Tissue Repair and Regenerative Medicine and Beijing Key Research Laboratory of Skin InjuryRepair and RegenerationResearch Unit of Trauma CareTissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China Research Unit of Trauma Care Tissue Repair and RegenerationChinese Academy of Medical Sciences2019RU051Beijing 100048China 2Department of Tissue Regeneration and Wound RepairChinese PLA General HospitalBeijing 100853China

Scar formation resulting from burns or severe trauma can significantly compromise the structural integrity of skin and lead to permanent loss of skin appendages,ultimately impairing its normal physiological *** evidence underscores the potential of targeted modulation of mechanical cues to enhance skin regeneration,promoting scarless repair by influencing the extracellular microenvironment and driving the phenotypic *** field of skin repair and skin appendage regeneration has witnessed remarkable advancements in the utilization of biomaterials with distinct physical ***,a comprehensive understanding of the underlying mechanisms remains somewhat elusive,limiting the broader application of these *** this review,we present two promising biomaterial-based mechanical approaches aimed at bolstering the regenerative capacity of compromised *** first approach involves leveraging biomaterials with specific biophysical properties to create an optimal scarless environment that supports cellular activities essential for *** second approach centers on harnessing mechanical forces exerted by biomaterials to enhance cellular plasticity,facilitating efficient cellular reprogramming and,consequently,promoting the regeneration of skin *** summary,the manipulation of mechanical cues using biomaterial-based strategies holds significant promise as a supplementary approach for achieving scarless wound healing,coupled with the restoration of multiple skin appendage functions.

关键词： Scarless Wound healing Biomaterials Mechanical cues Skin appendages

Efficient catalysis of FeNiCu-based multi-site alloys on magnesium-hydride for solid-state hydrogen storage

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Chinese Chemical Letters 2025年第1期36卷 603-609页

作者： shuai Li Liuting Zhang Fuying Wu Yiqun jiang Xuebin Yu School of Energy and Power Instrumental Analysis CenterJiangsu University of Science and TechnologyZhenjiang 212003China Department of Materials Science Fudan UniversityShanghai 200433China Max Planck Institute for Iron Research Düsseldorf 40237Germany

Hydrogen,as a cheap,clean,and cost-effective secondary energy source,performs an essential role in optimizing today’s energy *** hydride(Mg H_(2))represents an attractive hydrogen carrier for storage and transportation,however,the kinetic behavior and operating temperature remain *** this work,a dual-phase multi-site alloy(Ms A)anchored on carbon substrates was designed,and its superior catalytic effects on the hydrogen storage properties of MgH_(2) were *** analysis identified that multi-site Fe Ni_(3)/Ni Cu nanoalloys synergistically served as intrinsic drivers for the striking de/hydrogenation performance of the MgH_(2)-Ms A ***,the unique multi-metallic site structure attached to the surface of MgH_(2)provided substantial reversible channels and accessible active sites conducive to the adsorption,activation,and nucleation of H *** addition,the coupling system formed by FeNi_(3) and NiCu dual-phase alloys further enhanced the reactivity between Mg/MgH_(2) and H ***,the onset dehydrogenation temperature of Mg H_(2)+5 wt%Ms A was reduced to 195℃ and the hydrogen desorption apparent activation energy was reduced to 83.6 k J/mol.5.08 wt%H_(2) could be released at 250℃ in 20 min,reaching a high dehydrogenation rate of 0.254 wt%H_(2)/min,yet that for MgH_(2) at a higher temperature of 335℃ was only 0.145 wt%H_(2)/***,the dehydrogenated Mg H_(2)-Ms A sample could absorb hydrogen from room temperature(30℃)and charge 3.93 wt%H_(2) at 100℃ within20 min under 3.0 MPa H_(2) *** from carbon substrates,the 5 wt%Ms A doped-MgH_(2) could still maintain 6.36 wt%hydrogen capacity after 20 *** conclusion,this work provides experimental rationale and new insights for the design of efficient catalysts for magnesium-based solid-state hydrogen storage materials.

关键词： Hydrogen storage Magnesium hydride Synergistic effect Catalysis Multi-site alloys

Full genome analysis of swine genotype 3 hepatitis E virus isolated from eastern China

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第6期18卷 549-554页

作者： jiang-bing shuai Lu-huan LI Ai-yun LI Yong-qiang HE Xiao-feng ZHANG Zhejiang Academy of Science and Technology for Inspection and Quarantine Department of Cardiopulmonary Rehabilitation Hangzhou 128Hospital Women's Hospital School of MedicineZhejiang University

Hepatitis E is believed to occur in both endemic and sporadic forms in developing countries, which causes a major public health problem in Asia and Africa （Meng, 2010; Wang et al., 2016a）. Recent studies have docume... 详细信息

关键词：猪戊型肝炎病毒全基因组序列进化分析基因3型

Accumulation characteristics and fate modeling of phthalic acid esters in surface water from the Three Gorges Reservoir area,China

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Journal of Environmental Sciences 2025年第3期149卷 46-56页

作者： shuai Sun bing Zhang Junjie Hu Wen Gu ZhenWang Deling Fan Feng Ge Lili Shi LeiWang Nanjing Institute of Environmental Science Ministry of Ecology and Environment of the People’s Republic of ChinaNanjing 210042China Key Laboratory of Pesticide Environmental Assessment and Pollution Control Ministry of Ecology and Environment of the People’s Republic of ChinaNanjing 210042China Solid Waste and Chemicals Management Center Ministry of Ecology and Environment of the People’s Republic of ChinaBeijing 100029China

Phthalic acid esters(PAEs)are a group of compounds widespread in the *** investigate the occurrence and accumulation characteristics of PAEs,surface water samples were collected from the Three Gorges Reservoir area,*** total concentrations of∑_(11)analyzed PAEs(11PAEs)in the collected water samples ranging from 197.7 to 1,409.3 ng/L(mean±IQR:583.1±308.4 ng/L).While DEHP was the most frequently detected PAE,DnBP and DnNP were the most predominant PAEs in the analyzed water samples with a mean contribution of 63.3%of the∑_(11)*** concentrations of the∑_(11)PAEs in the water samples from the upper reaches of the Yangtze River were significantly higher than those from themiddle *** better understand the transport and fate of the PAEs,seven detected PAEs were modeled by Quantitative Water Air Sediment Interaction(QWASI).The simulated and measured values were close for most PAEs,and differences are within one order of magnitude even for the worst *** all simulated PAEs,water and particle inflow were main sources in the reservoir,whereas water outflow and degradation in water were important removal *** contribution ratios of different sources/losses varied fromPAEs,depending on their *** calculated risk quotients of DnNP in the Three Gorges Reservoir area whether based onmonitoring or simulating results were all far exceeded the safety threshold value,implying the occurrence of this PAE compound may cause potential adverse effects for the aquatic ecology of the Three Gorges Reservoir area.

关键词： Phthalic esters Surface water Transport and fate Three Gorges Reservoir area Risk assessment

The effect of fine grits and fine debris concentrations on the MLVSS/MLSS ratio of an activated sludge system

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Journal of Environmental Sciences 2025年第1期147卷 607-616页

作者： Zhao jiang Zhiheng Xia shuai Liu QiWei Haitao Fan Lu Qi Guohua Liu Hongchen Wang Low-carbon Water Environment Technology Research Center School of Environment&Natural ResourcesRenmin University of ChinaBeijing 100872China

This study embarks on an explorative investigation into the effects of typical concentrations and varying particle sizes of fine grits(FG,the involatile portion of suspended solids)and fine debris(FD,the volatile yet unbiodegradable fraction of suspended solids)within the influent on themixed liquor volatile suspended solids(MLVSS)/mixed liquor suspended solids(MLSS)ratio of an activated sludge *** meticulous experimentation,it was discerned that the addition of FG or FD,the particle size of FG,and the concentration of FD bore no substantial impact on the pollutant removal efficiency(denoted by the removal rate of COD and ammonia nitrogen)under constant operational ***,a notable decrease in the MLVSS/MLSS ratio was observed with a typical FG concentration of 20 mg/L,with smaller FG particle sizes exacerbating this ***,variations in FD concentrations influenced both MLSS andMLVSS/MLSS ratios;a higher FD concentration led to an increased MLSS and a reduced MLVSS/MLSS ratio,indicating FD accumulation in the system.A predictive model for MLVSS/MLSS was constructed based on quality balance calculations,offering a tool for foreseeing the MLVSS/MLSS ratio under stable long-term influent conditions of FG and *** model,validated using data from the BXH wastewater treatment plant(WWTP),showcased remarkable accuracy.

关键词： Fine grits and fine debris Activated sludge system MLVSS/MLSS ratio Predictive model

Achievable dual-strategy to stabilize Li-rich layered oxide interface by a one-step wet chemical reaction towards long oxygen redox reversibility

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Journal of Energy Chemistry 2025年第2期101卷 120-131页

作者： Bin He Yujie Dai shuai jiang Dawei Chen Xilong Wang Jie Song Dan Xiao Qian Zhao Yan Meng Wei Feng School of Mechanical Engineering Chengdu University Chongqing Institute of Metrology and Quality Inspection Institute of New Energy and Low-Carbon Technology Sichuan University

Oxygen release and electrolyte decomposition under high voltage endlessly exacerbate interfacial ramifications and structu ral degradation of high energy-density Li-rich layered oxide（LLO）,leading to voltage and capacity ***,the dual-strategy of Cr,B complex coating and local gradient doping is simultaneously achieved on LLO surface by a one-step wet chemical reaction at room *** functional theory（DFT） calculations prove that stable B-O and Cr-O bonds through the local gradient doping can significantly reduce the high-energy O 2p states of interfacial lattice O,which is also effective for the near-surface lattice O,thus greatly stabilizing the LLO surface,Besides,differential electrochemical mass spectrometry（DEMS） indicates that the CrxB complex coating can adequately inhibit oxygen release and prevents the migration or dissolution of transition metal ions,including allowing speedy Li+migration,The voltage and capacity fading of the modified cathode（LLO-CrB） are adequately suppressed,which are benefited from the uniformly dense cathode electrolyte interface（CEI） composed of balanced organic/inorganic ***,the specific capacity of LLO-CrB after 200 cycles at 1C is 209.3 mA h g-1（with a retention rate of 95.1%）.This dual-strategy through a one-step wet chemical reaction is expected to be applied in the design and development of other anionic redox cathode materials.

关键词： Interfacial oxygen Lithium-rich layered oxide cathode Local gradient doping One-step wet chemical reaction Surface coating

Melon2K array:A versatile 2K liquid SNP chip for melon genetics and breeding

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Horticultural Plant Journal 2025年第1期11卷 314-322页

作者： Qing Yu shuai Li Xiaofeng Su Xinxiu Chen Yuanhua Dong Zhiwang Yao Naiyu jiang Sen Chai Zhonghua Zhang Kuipeng Xu Engineering Laboratory of Genetic Improvement of Horticultural Crops of Shandong Province College of HorticultureQingdao Agriculture UniversityQingdaoShandong 266109China Key Laboratory of Special Food Proceing(Coconstructionby Ministry and Province) Ministryof Agriculture Rural AairsQingdao Agriculural UniversityQingdaoShandong 266109China Shandong Technology Innovation Center of Special Food QingdaoShandong 266109China Qingdao Special Food Research Institute QingdaoShandong 266109China Genome Analysis Laboratory of the Ministry of Agriculture Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhenGuangdong 518124China Institute of Vegetables and Flowers Chinese Academy of Agricultural SciencesKey Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of AgricultureSino-Dutch Joint Laboratory of Horticultural GenomicsBeijing 100081China

High-throughput genotyping tools can effectively promote molecular breeding in *** this study,genotyping by target sequencing(GBTS)system was utilized to develop a genome-wide liquid SNP chip for facilitating genetics and breeding in melon(Cucumis melo L.),a globally cultivated economically important horticultural *** on over eight million SNPs derived from 823 representative melon accessions,16K,8K,4K,2K,1K,500,250 and 125 informative SNPs were screened and evaluated for their polymorphisms,conservation of flanking sequences,and *** set of 2K SNPs was found to be optimal for representing the maximum diversity with the lowest number of SNPs,and it was selected to develop the liquid chip,named“Melon2K”.Using Melon2K,more than 1500 SNPs were detected across 17 samples of five melon cultivars,and the phylogenetic relationships were clearly *** the same cultivar,genetic differences were also assessed between different *** evaluated the performance of Melon2K in genetic background selection during the breeding process,obtaining the introgression lines of interested trait with more than 97%genetic background of elite variety by only two rounds of *** results suggest that Melon2K provides a cost-effective,efficient and reliable platform for genetic analysis and molecular breeding in melon.

关键词： Melon Cucumis melo L. Melon2K Liquid SNP chip Cultivar identification Background selection

Effect of h-BN rods on high-temperature properties of alumina-carbon refractory

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Journal of Iron and Steel Research International 2025年第1期32卷 115-128页

作者： Ya-ge Li shuai-bing Wu Feng Liang Zhong Huang Tu Long Long Dong Jun-yi Lv Hong-juan Duan Biao Wen Yong Liu Hai-jun Zhang The State Key Laboratory of Refractories and Metallurgy Wuhan University of Science and TechnologyWuhan430081HubeiChina Sinosteel Luoyang Institute of Refractories Research Co. Ltd.Luoyang471039HenanChina

h-BN rods modified low-carbon alumina-carbon(Al_(2)O_(3)-C)refractories were prepared,and the effect of h-BN rod addition on the high-temperature properties was investigated and compared with commercial h-BN flake,carbon black,and carbon nanotubes *** results demonstrated that Al_(2)O_(3)-C refractories containing h-BN rods exhibited optimal high-temperature service performances,including 25%higher hot modulus of rupture,21.3%higher thermal shock strength residual ratio,20.9%lower in oxidation and 44.3%less in slag corrosion,compared to the counterpart specimens without ***,benefiting from the synergistic enhancement of the rod-like h-BN and in-situ generated SiC whiskers,the high-temperature service performances of h-BN rods containing specimens outperformed counterpart specimens containing commercial h-BN flake,carbon black,and carbon nanotubes,respectively.

关键词： Al_(2)O_(3)-C refractory h-BN rod Microstructure High-temperature service performance