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The loss-of-function mutations and down-regulated expression of ASB3 gene promote the growth and metastasis of colorectal cancer cells

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Chinese Journal of Cancer 2017年第1期36卷 11-25页

作者： wu-Ying Du Zhen-Hai Lu Wen Ye Xiang Fu Yi Zhou Chun-Mei Kuang jiang-xue wu Zhi-Zhong Pan Shuai Chen Ran-Yi Liu Wen-Lin Huang State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer Medicine Sun Yat-sen University Cancer Center Department of Radiation Oncology University of Arkansas for Medical Sciences Department of Oncology The First Affiliated Hospital Sun Yat-sen University Department of Radio-chemotherapy Shangrao People’s Hospital Guangdong Provincial Key Laboratory of Tumor-targeted Drug and Guangzhou Enterprise Key Laboratory of Gene Medicine Guangzhou Doublle Bioproducts Co. Ltd.

Background: Ankyrin repeat and SOCS box protein 3(ASB3) is a member of ASB family and contains ankyrin repeat sequence and SOCS box domain. Previous studies indicated that it mediates the ubiquitination and degradation of tumor necrosis factor receptor 2 and is likely involved in inflammatory responses. However, its effects on oncogenesis are unclear. This study aimed to investigate the effects of ASB3 on the growth and metastasis of colorectal cancer(CRC).Methods: We used next?generation sequencing or Sanger sequencing to detect ASB3 mutations in CRC specimens or cell lines, and used real?time quantitative polymerase chain reaction, Western blotting, and immunohistochemical or immunofluorescence assay to determine gene expression. We evaluated cell proliferation by MTT and colony for?mation assays, tested cell cycle distribution by flow cytometry, and assessed cell migration and invasion by transwell and wound healing assays. We also performed nude mouse experiments to evaluate tumorigenicity and hepatic metastasis potential of tumor ***: We found that ASB3 gene was frequently mutated(5.3%) and down?regulated(70.4%) in CRC cases. Knock?down of endogenous ASB3 expression promoted CRC cell proliferation, migration, and invasion in vitro and facilitated tumorigenicity and hepatic metastasis in vivo. Conversely, the ectopic overexpression of wild?type ASB3, but not that of ASB3 mutants that occurred in clinical CRC tissues, inhibited tumor growth and metastasis. Further analysis showed that ASB3 inhibited CRC metastasis likely by retarding epithelial?mesenchymal transition, which was characterized by the up?regulation of β?catenin and E?cadherin and the down?regulation of transcription factor 8, N?cadherin, and ***: ASB3 dysfunction resulted from gene mutations or down?regulated expression frequently exists in CRC and likely plays a key role in the pathogenesis and progression of CRC.

关键词： Ankyrin repeat and SOCS box protein 3(ASB3) Colorectal cancer Epithelial-mesenchymal transition Cell proliferation Tumor metastasis

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Effect of simvastatin on the atherosclerotic plaque stability and the angiogenesis in the atherosclerotic plaque of rabbits

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Chinese Science Bulletin 2009年第15期54卷 3061-3066页

作者： ZHANG Lu jiang YueRong xue Mei wu CaiFeng WANG JingShang YIN HuiJun Department of Cardiovascular Disease Xiyuan Hospital China Academy of Chinese Medical Sciences Beijing 100091 China

In this study, the effect of simvastatin on the atherosclerotic plaque stability and the angiogenesis in the atherosclerotic plaque of rabbits was observed. Thirty New Zealand rabbits were randomly divided into the normal control group, the model control group and the simvastatin group, 10 in each group. Rabbits in the normal control group were fed with normal forage, while rabbits in the rest two groups were fed with high fat forage. The balloon injury was performed two weeks later to establish an abdominal aortic atherosclerosis model, and then high fat forage was successively fed to them. Meanwhile, simvastatin at the daily dose of 2.5 mg/kg body weight was administered to rabbits in the simvastatin group. After 6 weeks of successive administration, levels of blood lipids were measured after blood sampling, and the serum high sensitivity C-reactive protein (hsCRP) and matrix metalloproteinase-3,-9 (MMP-3,-9) were detected using enzyme-linked immunosorbent assay. The macroscopically pathological indices of the plaque tissue were observed using hematoxylin and eosin (HE) staining of abdominal aorta specimens under a light microscope, and the plaque area (PA), cross-sectional vascular area (CVA) and correcting plaque area (PA/CVA) were determined quantitatively using imaging software. The protein expressions of the vascular endothelial growth factor (VEGF), factor VIII related antigen (FVIIIRAg), MMP-3 and cluster of differentiation antigen 40 ligand (CD40L) in the plaque were detected with the immunohistochemical method. Compared with the model control group, the levels of VEGF, FVIIIRAg, MMP-3, CD40L protein expression and the serum expression levels of hsCRP, MMP-3, MMP-9 in the simvastatin group were significantly reduced (P<0.05, P<0.01). The ratio PA/CVA in the simvastatin group was more significantly reduced when compared with that in the model control group (P<0.01). The levels of serum total cholesterol (TC), triglyceride (TG) and low-density lipoprotein ch

关键词：粥样动脉硬化血管发生稳定性医学动物实验

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Endovascular Treatment of the Huge Dissecting Aneurysms Involving the Basilar Artery by the Internal Trapping Technique： Technical Note

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Chinese Medical Journal 2015年第14期128卷 1916-1921页

作者： Shi-Qing Mu Xin-Jian Yang You-Xiang Li Chu-Han jiang Zhong-xue wu Department of Interventional Neuroradiology Beijing Neurosurgical Institute and Beijing Tiantan Hospital Capital Medical University Beijing 100050 China

Background： The endovascular strategy of the huge dissecting aneurysms involving the basilar artery （BA） is controversial and challenging. This study was to investigate the clinical and angiographic outcomes of the treatment of the huge dissecting aneurysms involving the BA by the internal trapping （IT） technique. Methods： We retrospectively studied 15 patients with the huge dissecting aneurysms involving the BA treated by the IT technique between September 2005 and September 2014 in Department of Interventional Neuroradiology of Beijing Tiantan Hospital. Clinical and angiographic data were reviewed and evaluated. Results： All patients were treated by the IT technique. That meant the dissecting artery and aneurysm segments were completed occlusion. After the procedure, the angiography demonstrated that all the dissecting artery and aneurysm segments were completed occlusion. Follow-up angiography was performed at 3-6 months or 12-18 months alter the endovascular treatment （median 8 months）, 14 patients had a good recovery. Re-canalization occurred in one patient whose aneurysm involved in bilateral vertebral arteries and the two third of the middle-lower BA. After the second treatment, the patient died by the ventricular tachycardia. Conclusions： The IT technique is a technically feasible and safe alternative for the treatment of BA dissecting aneurysms, but it is not necessarily the safest or most definitive treatment modality. The ideal treatment of the huge dissecting aneurysms involving the BA remains debatable and must be investigated on a case-by-case basis.

关键词： Basilar Artery Endovascular Treatment Huge Dissecting Aneurysms Internal Trapping Technique

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哈佛大学医学院转化医学实践的启示

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中华医学杂志 2010年第22期90卷 1519-1521页

作者：蒋学武汕头大学医学院 515041

目前,转化医学（translational medicine）已为国内外学者的广泛关注和认同,但其确切定义却尚未完全形成,对其理解也各有不同.因此,当我们面临实践转化医学的时候,有必要了解国际一流医学院的理念和做法.

关键词：哈佛大学医学院转化医学医学实践国内外临实践

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Human umbilical cord Wharton's Jelly-derived mesenchymal stem cells differentiation into nerve-like cells

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Chinese Medical Journal 2005年第23期118卷 1987-1993页

作者： MA Lian FENG xue-yong CUI Bing-lin Frieda Law jiang xue-wu YANG Li-ye XIE Qing-dong HUANG Tian-hua Department of Pediatrics Second Affiliated Hospital of ShantouUniversity Medical College Shantou 515041 China Shantou University Medical College Shantou 515041 China Department of Neurosurgery Chaozhou Central Hospital Chaozhou521021 China Research Center for Reproductive Medicine of Shantou UniversityMedical College Shantou 515041 China

Background The two most basic properties of mesenchymal stem cells （MSCs） are the capacities to selfrenew indefinitely and differentiate into multiple cells and tissue types. The cells from human umbilical cord Wharton＇ s Jelly have properties of MSCs and represent a rich source of primitive cells. This study was conducted to explore the possibility of inducing human umbilical cord Wharton＇ s Jelly-derived MSCs to differentiate into nerve-like cells.Methods MSCs were cultured from the Wharton＇ s Jelly taken from human umbilical cord of babies delivered after full-term normal labor. Salvia miltiorrhiza and [3-mercaptoethanol were used to induce the human umbilical cord-derived MSCs to differentiate The expression of neural protein markers was shown by immunocytochemistry. The induction process was monitored by phase contrast microscopy, electron microscopy （EM）, and laser scanning confocal microscopy （LSCM） . The pleiotrophin and nestin genes were measured by reverse transcription-polymerase chain reaction （RT-PCR）.Results MSCs in the Wharton＇ s Jelly were easily attainable and could be maintained and expanded in culture. They were positive for markers of MSCs, but negative for markers of hematopoietic cells and graft-versus-host disease （GVHD）-related cells. Treatment with Salvia mihiorrhiza caused Wharton＇ s Jelly cells to undergo profound morphological changes. The induced MSCs developed rounded cell bodies with multiple neurite-like extensions. Eventually they developed processes that formed networks reminiscent of primary cultures of neurons. Salvia mihiorrhiza and β-mercaptoethanol also induced MSCs to express nestin, β-tubulin Ⅲ, neurofilament （NF） and glial fibrillary acidic protein （GFAP）. It was confirmed by RT-PCR that MSCs could express pleiotrophin both before and after induction by Salvia miltiorrhiza. The expression was markedly enhanced after induction and the nestin gene was also expressed.Conclusions MSCs could be isolated from human umbilical

关键词： Wharton' s Jelly · mesenchymal stem cells ·differentiation ·nerve cells· Salvia miltiorrhiza

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基于结构优化递归神经网络的网络流量预测

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西南大学学报（自然科学版） 2016年第2期38卷 149-154页

作者：江务学东莞职业技术学院计算机工程系广东东莞523808

针对传统方法预测网络流量精度较低的问题,提出了一种基于改进双线性递归神经网络模型(BLRNN)的非线性网络流量预测方法.首先,给出了双线性递归神经网络的定义及网络结构描述;然后从网络结构和修剪过程两方面对双线性递归神经网络进行... 详细信息

针对传统方法预测网络流量精度较低的问题,提出了一种基于改进双线性递归神经网络模型(BLRNN)的非线性网络流量预测方法.首先,给出了双线性递归神经网络的定义及网络结构描述;然后从网络结构和修剪过程两方面对双线性递归神经网络进行优化改进,采用遗传算法全局搜索进行修剪;最后,通过真实工况下的网络流量数据用仿真试验对模型性能进行分析.试验结果表明,优化后双线性递归神经网络模型大幅降低了算法复杂度,提高了计算效率,与传统的多层神经网络预测方法相比,该方法预测精度更高.同时,该方法也为其他具有相似特征的非线性预测问题提供了一种新的研究思路.

关键词：神经网络结构优化遗传算法网络流量预测剪枝优化算法

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儿童泌尿系结石处理的证据医学

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实用儿科临床杂志 2011年第11期26卷 816-819页

作者：蒋学武汕头大学医学院第二附属医院小儿外科广东汕头515041

儿童泌尿系结石无论是病因还是诊疗策略都有其自身的特点,因为发病率较成人相对较低,所以研究相对较少,积累的经验也相对缺乏。现主要基于欧美国家有关儿童泌尿系结石指南的有关资料,根据循证医学的思想,介绍目前常用儿童泌尿系结石处... 详细信息

儿童泌尿系结石无论是病因还是诊疗策略都有其自身的特点,因为发病率较成人相对较低,所以研究相对较少,积累的经验也相对缺乏。现主要基于欧美国家有关儿童泌尿系结石指南的有关资料,根据循证医学的思想,介绍目前常用儿童泌尿系结石处理方法的证据水平或推荐强度,供临床研究和实践参考。

关键词：泌尿系结石证据医学诊断治疗儿童

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完善我国收入分配机制的经济法思考

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河北法学 2008年第8期26卷 35-38页

作者：薛江武中南大学湖南长沙410083

法律是社会利益资源和权利的分配书。解决当前我国日趋尖锐复杂的收入分配问题,应该大力发挥经济法在分配功能。经济法在实现再分配时应遵循利益平衡原则、社会整体利益原则和有限调节原则。发挥经济法的再分配功能,建立科学分配机制,... 详细信息

法律是社会利益资源和权利的分配书。解决当前我国日趋尖锐复杂的收入分配问题,应该大力发挥经济法在分配功能。经济法在实现再分配时应遵循利益平衡原则、社会整体利益原则和有限调节原则。发挥经济法的再分配功能,建立科学分配机制,要处理好经济增长与收入分配的关系,加大利用财税杠杆调节收入分配的力度,进一步完善劳动工资立法,进一步完善社会保障法律体系,公平分配社会教育资源,缩小城乡教育投入的差距,并进一步强化经济司法。

关键词：经济法收入分配公平再分配

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关于构建“三层级医疗服务体系”的国家医疗改革设想

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中国卫生事业管理 2013年第1期30卷 6-8页

作者：蒋学武汕头大学医学院广东汕头515041

文章结合医疗服务主要由三层级医疗构成的特性和国内现实状况以及国际先进经验,建议因势利导地构建一个相对简单、易于管理的国家"三层级医疗服务体系",包括国家级学术医学中心,省级(区域)医疗中心和基层医疗(卫生)服务网点。并初步阐... 详细信息

文章结合医疗服务主要由三层级医疗构成的特性和国内现实状况以及国际先进经验,建议因势利导地构建一个相对简单、易于管理的国家"三层级医疗服务体系",包括国家级学术医学中心,省级(区域)医疗中心和基层医疗(卫生)服务网点。并初步阐释了其意义及实现方法。希望有助于我国现行公立医疗系统的改革。

关键词：医药卫生体制改革三层级医疗学术医学中心区域医疗中心初级卫生保健

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Preparation of Co/S co-doped carbon catalysts for excellent methylene blue degradation

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 169-181页

作者： Haixu Li Haobo He Tiannan jiang Yunfei Du Zhichen wu Liang Xu Xinjie Wang Xiaoguang Liu Wanhua Yu Wendong xue School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China Beijing Jinyu Tongda Refractory Technologies Co. Ltd.Beijing 100085China

S and Co co-doped carbon catalysts were prepared via pyrolysis of MOF-71 and thiourea mixtures at 800℃at a mass ratio of MOF-71 to thiourea of 1:0.1 to effectively activate peroxymonosulfate(PMS)for methylene blue(MB)*** effects of two different mixing routes were identified on the MB degradation ***,the catalyst obtained by the alcohol solvent evaporation(MOF-AEP)mixing route could degrade 95.60%MB(50 mg/L)within 4 min(degradation rate:K=0.78 min^(-1)),which was faster than that derived from the direct grinding method(MOF-DGP,80.97%,K=0.39 min^(-1)).X-ray photoelectron spectroscopy revealed that the Co-S content of MOF-AEP(43.39at%)was less than that of MOF-DGP(54.73at%),and the proportion of C-S-C in MOF-AEP(13.56at%)was higher than that of MOF-DGP(10.67at%).Density functional theory calculations revealed that the adsorption energy of Co for PMS was -2.94 eV when sulfur was doped as C-S-C on the carbon skeleton,which was higher than that when sulfur was doped next to cobalt in the form of Co-S bond(-2.86 eV).Thus,the C-S-C sites might provide more contributions to activate PMS compared with ***,the degradation parameters,including pH and MOF-AEP dosage,were ***,radical quenching experiments and electron paramagnetic resonance(EPR)measurements revealed that ^(1)O_(2)might be the primary catalytic species,whereas·O~(2-)might be the secondary one in degrading MB.

关键词： advanced oxidation process alcohol solvent evaporation hydrogen bond S and Co co-doped carbon catalysts wastewater remediation

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