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Transgenic barley with overexpressed PTrx increases aluminum resistance in roots during germination

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第11期11卷 862-870页

作者： Qiao-yun LI Hong-bin NIU Jun YIN Hong-bo SHAO Ji-shan NIU jiang-ping ren Yong-chun LI Xiang WANG National Engineering Research Center for Wheat Henan Agricultural University Zhengzhou 450002 China Yantai Institute of Coastal Zone Research Chinese Academy of Sciences Yantai 264003 China Institute of Life Sciences Qingdao University of Science and Technology (QUST) Qingdao 266042 China

A transgenic barley line(LSY-11-1-1) with overexpressed Phalaris coerulescens thioredoxin gene(PTrx) was employed to measure the growth,protein oxidation,cell viability,and antioxidase activity in barley roots during germination on the presence of 2 mmol/L AlCl3 on filter *** results show that(1) compared with the non-transgenic barley,LSY-11-1-1 had enhanced root growth,although both were seriously inhibited after AlCl3 treatment;(2) the degree of protein oxidation and loss of cell viability in roots of LSY-11-1-1 were much less than those in roots of non-transgenic barley,as reflected by lower contents of protein carbonyl and Evans blue uptakes in LSY-11-1-1;(3) activities of catalase(CAT) ,glutathione peroxidase(GPX) ,ascorbate peroxidase(APX) ,and glu-tathione reductase(GR) in LSY-11-1-1 root tips were generally higher than those in non-transgenic barley root tips,although these antioxidase activities gave a rise to different degrees in both LSY-11-1-1 and non-transgenic barley under aluminum *** results indicate that overexpressing PTrx could efficiently protect barley roots from oxidative injury by increasing antioxidase activity,thereby quenching ROS caused by AlCl3 during *** properties raise the possibility that transgenic barley with overexpressed PTrx may be used to reduce the aluminum toxicity in acid soils.

关键词： Phalaris coerulescens thioredoxin gene (PTrx) Aluminum Transgenic barley Oxidative stress

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The Level of Expression of Thioredoxin is Linked to Fundamental Properties and Applications of Wheat Seeds

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Molecular Plant 2009年第3期2卷 430-441页

作者： Yong-Chun Li jiang-ping ren Myeong-Je Cho Su-Mei Zhou Yong-Bum Kim Hong-Xiang Guo Joshua H. Wong Hong-Bin Niu Hyun-Kyung Kim Susumu Morigasaki Peggy G. Lemaux Oscar L. Frick Jun Yin Bob B. Buchanan National Engineering Research Centre for Wheat Henan Agricultural University Zhengzhou 450002 China Department of Plant and Microbial Biology 111 Koshland Hall University of California Berkeley CA 94720 USA Department of Pediatrics School of Medicine University of California San Francisco CA 94143 USA

Work with cereals （barley and wheat） and a legume （Medicago truncatula） has established thioredoxin h （Trx h） as a central regulatory protein of seeds. Trx h acts by reducing disulfide （S-S） groups of diverse seed proteins （storage proteins, enzymes, and enzyme inhibitors）, thereby facilitating germination. Early in vitro protein studies were complemented with experiments in which barley seeds with Trx h overexpressed in the endosperm showed accelerated germination and early or enhanced expression of associated enzymes （α-amylase and pullulanase）. The current study extends the transgenic work to wheat. Two approaches were followed to alter the expression of Trx h genes in the endosperm：（1） a hordein promoter and its protein body targeting sequence led to overexpression of Trx hS, and （2） an antisense construct of Trx h9 resulted in cytosolic underexpression of that gene （Arabidopsis designation）. Underexpression of Trx h9 led to effects opposite to those observed for overexpression Trx h5 in barley--retardation of germination and delayed or reduced expression of associated enzymes. Similar enzyme changes were observed in developing seeds. The wheat lines with underexpressed Trx showed delayed preharvest sprouting when grown in the greenhouse or field without a decrease in final yield. Wheat with overexpressed Trx h5 showed changes commensurate with earlier in vitro work： increased solubility of disulfide proteins and lower aUergenicity of the gliadin fraction. The results are further evidence that the level of Trx h in cereal endosperm determines fundamental properties as well as potential applications of the seed.

关键词： metabolic regulation molecular physiology seed biology preharvest sprouting seed germination thioredoxin h wheat allergenicity.

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红豆树百年老林分的主要生态特征值研究

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福建林业科技 2007年第1期34卷 33-35,39页

作者：郑天汉黄昌尧蔡勇江仁平福建省林业厅世行办福建福州350003 屏南县林业局福建屏南352300 延平区溪后采育场福建南平353000 古田县平湖林业站福建古田352200

分析红豆树(Ormosia hosiei)百年以上老林分的11个乔木层树种的相对优势度、物种多样性、均匀度、生态位宽度、生态位重叠度等指标。红豆树在该生态环境中比其它树种的适应性都好。红豆树种群稳定性较好,榕冬青次之,其它树种尚处群落发... 详细信息

分析红豆树(Ormosia hosiei)百年以上老林分的11个乔木层树种的相对优势度、物种多样性、均匀度、生态位宽度、生态位重叠度等指标。红豆树在该生态环境中比其它树种的适应性都好。红豆树种群稳定性较好,榕冬青次之,其它树种尚处群落发育初期,演替趋势不稳定。

关键词：红豆树老林分生态特征值

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复杂荷载条件下变刚度多层框架码头设计优化

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水运工程 2016年第11期 72-76,104页

作者：王文华高加云许智鸿姜仁萍中船第九设计研究院工程有限公司上海200063 上海海洋工程和船厂水工特种工程技术研究中心上海200063

以常德达门船舶有限公司吊机码头工程为例,建立多个框架码头三维有限元模型,在分析研究变刚度多层框架码头承受不同位置船舶水平撞击力下的变形特性的基础上,通过对比有无竖向斜撑、水平斜撑等方案对码头整体变形的影响,提出增加竖向斜... 详细信息

以常德达门船舶有限公司吊机码头工程为例,建立多个框架码头三维有限元模型,在分析研究变刚度多层框架码头承受不同位置船舶水平撞击力下的变形特性的基础上,通过对比有无竖向斜撑、水平斜撑等方案对码头整体变形的影响,提出增加竖向斜撑而不增加水平斜撑的优化方案。该优化减少了复杂荷载条件下框架码头结构的变形,在有限的工程投资下,增加了结构的整体性,提高了结构的可靠度。

关键词：框架码头复杂荷载变刚度优化竖向斜撑水平斜撑

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Iron-handling solute carrier SLC22A17 as a blood-brain barrier target after stroke

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Neural Regeneration Research 2025年第11期20卷 3207-3208页

作者： Xueqi ren Wenlu Li Key Laboratory of Neuropharmacology and Translational Medicine of Zhejiang Province School of Pharmaceutical SciencesZhejiang Chinese Medical UniversityHangzhouZhejiang ProvinceChina Neuroprotection Research Laboratories Departments of Radiology and NeurologyMassachusetts General HospitalHarvard Medical SchoolBostonMAUSA

The pathophysiology of ischemic stroke is complex and multifactorial,involving various forms of cell death such as apoptosis,autophagy,and necrosis.A recent study suggests that oxidative and inflammatory stress can induce ferroptosis,a specialized form of cell death characterized by the accumulation of lipid peroxides dependent on intracellular iron overload(Li and Jia,2023).

关键词： death specialized barrier

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Optimizing the key parameter to accelerate the recovery of AMOC under a rapid increase of greenhouse gas forcing

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Atmospheric and Oceanic Science Letters 2025年第1期18卷 39-45页

作者： Haolan ren Fei Zheng Tingwei Cao Qiang Wang International Center for Climate and Environment Science(ICCES) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina College of Oceanography Hohai UniversityNanjingChina CAS Key Laboratory of Ocean Circulation and Waves Institute of OceanologyChinese Academy of Sciences and Pilot National Laboratory for Marine Science and Technology(Qingdao)QingdaoChina

Atlantic Meridional Overturning Circulation(AMOC)plays a central role in long-term climate variations through its heat and freshwater transports,which can collapse under a rapid increase of greenhouse gas forcing in climate *** studies have suggested that the deviation of model parameters is one of the major factors in inducing inaccurate AMOC *** this work,with a low-resolution earth system model,the authors try to explore whether a reasonable adjustment of the key model parameter can help to re-establish the AMOC after its *** a new optimization strategy,the extra freshwater flux(FWF)parameter is determined to be the dominant one affecting the AMOC’s *** traditional ensemble optimal interpolation(EnOI)data assimilation and new machine learning methods are adopted to optimize the FWF parameter in an abrupt 4×CO_(2) forcing experiment to improve the adaptability of model parameters and accelerate the recovery of *** results show that,under an abrupt 4×CO_(2) forcing in millennial simulations,the AMOC will first collapse and then re-establish by the default FWF parameter ***,during the parameter adjustment process,the saltier and colder sea water over the North Atlantic region are the dominant factors in usefully improving the adaptability of the FWF parameter and accelerating the recovery of AMOC,according to their physical relationship with FWF on the interdecadal timescale.

关键词： Recovery of AMOC 4×CO_(2)forcing Key parameter Parameter estimation Data assimilation Machine learning

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Multi-physics multi-scale simulation of unique equiaxed-to-columnar-to-equiaxed transition during the whole solidification process of Al-Li alloy laser welding

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Journal of Materials Science & Technology 2024年第4期171卷 235-251页

作者： Chu Han ping jiang Shaoning Geng Liangyuan ren School of Mechanical Science and Engineering Huazhong University of Science&Technology(HUST)Wuhan430074China

In this study,a novel multi-physics multi-scale model with the dilute multicomponent phase-field method in three-dimensional(3D)space was developed to investigate the complex microstructure evolu-tion in the molten pool during laser welding of Al-Li *** accurately compute mass data within both two and three-dimensional computational domains,three efficient computing methods,including central processing unit parallel computing,adaptive mesh refinement,and moving-frame algorithm,were *** was placed on the distinctive equiaxed-to-columnar-to-equiaxed transition phenomenon that occurs during the entire solidification process of Al-Li alloy laser *** results indi-cated that the growth distance of columnar grains that epitaxially grew from the base metal(BM)de-creased as the nucleation rate *** the nucleation rate increased,the morphology of the newly formed grains near the fusion boundary(FB)changed from columnar to equiaxed,and newly formed equiaxed grains changed from having high-order dendrites to no obvious dendrite *** the nucleation rate was sufficiently high,non-dendritic equiaxed grains could directly form near the FB,and there was nearly no epitaxial growth from the ***,simulation results illustrated the com-petition among multiple grains with varying orientations that grow in 3D space near the ***,how equiaxed grain bands develop was *** equiaxed band not only hindered the growth of early columnar grains but also some of its grains could grow epitaxially to form new columnar *** predicted results were in good agreement with experimental measurements and observations.

关键词： Laser welding Al-Li alloy Equiaxed-to-columnar-to-equiaxed transition Multi-physics multi-scale model Multicomponent alloys 3D phase-field model

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水平荷载作用下变刚度多层框架码头空间特性及分配系数

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水运工程 2017年第3期 51-57页

作者：王文华姜仁萍高加云陈隽祎俞梅欣袁超中船第九设计研究院工程有限公司上海200063 上海海洋工程和船厂水工特种工程技术研究中心上海200063

采用桩基剪力法、弹性支撑刚性梁法等多种方法对水平荷载作用下的变刚度多层框架码头的空间特性及排架分配系数进行计算与比较分析,结果表明:桩基剪力法能更好地反映出结构的空间特性;在排架刚度变化幅度不大的情况下,弹性支撑刚性梁法... 详细信息

采用桩基剪力法、弹性支撑刚性梁法等多种方法对水平荷载作用下的变刚度多层框架码头的空间特性及排架分配系数进行计算与比较分析,结果表明:桩基剪力法能更好地反映出结构的空间特性;在排架刚度变化幅度不大的情况下,弹性支撑刚性梁法的计算结果与规范中的排架分配系数接近。在此基础上,通过改变大刚度排架的刚度值及位置,得出排架间刚度差异、大刚度排架位置与分配系数的相互关系;分析空间特性及面板刚度对水平分配系数的影响。

关键词：框架码头变刚度分配系数空间特性桩基剪力法弹性支撑刚性梁法

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因地制宜发展新质生产力的学理阐释

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新疆师范大学学报（哲学社会科学版） 2025年第2期46卷 39-47页

作者：李传兵任平阳贵州大学长征文化研究院贵州贵阳550025 贵州大学中共党史党建研究院贵州贵阳550025 贵州大学马克思主义学院贵州贵阳550025 贵州大学哲学学院贵州贵阳550025

因地制宜发展新质生产力是我国经济发展领域的重要课题,科学合理发展新质生产力是推动经济持续增长和提升全民福祉的关键所在。文本在系统把握因地制宜中的“时”“业”“域”特点,深入挖掘因地制宜发展新质生产力理论内涵的基础上,从... 详细信息

因地制宜发展新质生产力是我国经济发展领域的重要课题,科学合理发展新质生产力是推动经济持续增长和提升全民福祉的关键所在。文本在系统把握因地制宜中的“时”“业”“域”特点,深入挖掘因地制宜发展新质生产力理论内涵的基础上,从马克思主义哲学角度与中国特色社会主义建设的具体实践角度探讨其生成依据,围绕制度、发展、主体、创新四个向度阐述因地制宜发展新质生产力的价值旨归。通过对立足地方实际、直面地方问题、汇聚地方力量和激发地方活力四个方面的实践探赜,重点揭示如何将因地制宜发展新质生产力理论转化为可落地的务实行动,推动新质生产力进一步发展。

关键词：新质生产力因地制宜高质量发展实事求是学理阐释

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Tailoring Light–Matter Interactions in Overcoupled Resonator for Biomolecule Recognition and Detection

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Nano-Micro Letters 2025年第1期17卷 262-280页

作者： Dongxiao Li Hong Zhou Zhihao ren Cheng Xu Chengkuo Lee Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore Center for Intelligent Sensors and MEMS(CISM) National University of SingaporeSingapore 117608Singapore

Plasmonic nanoantennas provide unique opportunities for precise control of light–matter coupling in surface-enhanced infrared absorption(SEIRA)spectroscopy,but most of the resonant systems realized so far suffer from the obstacles of low sensitivity,narrow bandwidth,and asymmetric Fano resonance ***,we demonstrated an overcoupled resonator with a high plasmon-molecule coupling coefficient(μ)(OC-Hμresonator)by precisely controlling the radiation loss channel,the resonator-oscillator coupling channel,and the frequency detuning *** observed a strong dependence of the sensing performance on the coupling state,and demonstrated that OC-Hμresonator has excellent sensing properties of ultra-sensitive(7.25%nm^(−1)),ultra-broadband(3–10μm),and immune asymmetric Fano *** characteristics represent a breakthrough in SEIRA technology and lay the foundation for specific recognition of biomolecules,trace detection,and protein secondary structure analysis using a single array(array size is 100×100μm^(2)).In addition,with the assistance of machine learning,mixture classification,concentration prediction and spectral reconstruction were achieved with the highest accuracy of 100%.Finally,we demonstrated the potential of OC-Hμresonator for SARS-CoV-2 *** findings will promote the wider application of SEIRA technology,while providing new ideas for other enhanced spectroscopy technologies,quantum photonics and studying light–matter interactions.

关键词： Plasmonic nanoantennas Light-matter interaction Surface-enhanced infrared absorption Overcoupled Biosensing

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