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Down-regulation of miR-155 inhibits inflammatory response in human pulmonary microvascular endothelial cells infected with influenza A virus by targeting sphingosine-1-phosphate receptor 1

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Chinese Medical Journal 2020年第20期133卷 2429-2436页

作者： Si-Mei Shen Hao jiang jiang-nan zhao Yi Shi Department of Respiratory and Critical Care Medicine Jinling HospitalMedical School of Nanjing UniversityNanjingJiangsu 210016China Department of Emergency Medicine The Second Affiliated HospitalSoutheast UniversityNanjingJiangsu 210003China

Background:Endothelial cells play a key role in the cytokine storm caused by influenza A virus. MicroRNA-155 (miR-155) is an important regulator in inflammation. Its role in the inflammatory response to influenza A infection, however, has yet to be elucidated. In this study, we explored the role as well as the underlying mechanism of miR-155 in the cytokine production in influenza A-infected endothelial cells.Methods:Human pulmonary microvascular endothelial cells (HPMECs) were infected with the influenza A virus strain H1N1. The efficiency of H1N1 infection was confirmed by immunofluorescence. The expression levels of proinflammatory cytokines and miR-155 were determined using real-time polymerase chain reaction. A dual-luciferase reporter assay characterized the interaction between miR-155 and sphingosine-1-phosphate receptor 1 (S1PR1). Changes in the target protein levels were determined using Western blot analysis.Results:MiR-155 was elevated in response to the H1N1 infection in HPMECs (24 h post-infection vs. 0 h post-infection, 3.875 ± 0.062 vs. 1.043 ± 0.013, P = 0.001). Over-expression of miR-155 enhanced inflammatory cytokine production (miR-155 mimic vs. negative control, all P < 0.05 in regard of cytokine levels) and activation of nuclear factor kappa B in infected HPMECs (miR-155 mimic vs. negative control, P = 0.004), and down-regulation of miR-155 had the opposite effect. In addition, S1PR1 was a direct target of miR-155 in the HPMECs. Inhibition of miR-155 enhanced the expression of the S1PR1 protein. Down-regulation of S1PR1 decreased the inhibitory effect of the miR-155 blockade on H1N1-induced cytokine production and nuclear factor kappa B activation in HPMECs. Conclusion:MiR-155 maybe modulate influenza A-induced inflammatory response by targeting S1PR1.

关键词： MicroRNA-155 Sphingosine 1-phosphate receptor 1 Influenza A virus Endothelial cells

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Correlation among academic self-efficacy,professional identity,and professional attitude of higher vocational nursing students

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护理前沿（英文） 2021年第1期8卷 43-47页

作者： Tian-Liang Xu jiang-nan zhao Yan-Yan Zhang Gai-Li Jing Department of Nursing Henan Medical CollegeZhengzhouHenan 450003China Medical College Zhengzhou University of Industry TechnologyZhengzhouHenan 451150China

Objective:To analyze the influence of professional identity and academic efficacy on the professional attitude of higher vocational nursing students.Methods:A total of 656 higher vocational nursing students were investigated with general information questionnaire,academic self-efficacy scale,professional identity scale,and professional attitude scale of nursing students.Results:The score of professional attitude of higher vocational nursing students was(106.34±9.86),which is found to be in the upper middle level.The professional attitude of higher vocational nursing students is positively correlated with academic self-efficacy(P<0.05),and with learning ability self-efficacy(P<0.01).Professional cognition,professional will,and professional values have a positive predictive effect on the professional attitude of higher vocational nursing students(P<0.05).Conclusions:Academic self-efficacy and professional identity are important predictors of professional attitude.Learning behavior self-efficacy,professional cognition,professional will,and professional values have a significant impact on the establishment of positive professional attitude.

关键词： vocational nursing students professional attitude academic self-efficacy professional identity correlation

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计算美学的生成与度量

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湖北第二师范学院学报 2024年第6期41卷 91-97页

作者：汪贤俊江昭楠集美大学艺术与设计学院福建厦门361021

计算美学作为一门跨学科研究领域,旨在探讨计算机科学与艺术的交汇点,让计算机得以像人类一样做出审美决策,包含美学的生成和度量两个方向,核心在于研究美的本质及如何利用计算机技术来实现和衡量美。计算美学的生成即利用计算机生成具... 详细信息

计算美学作为一门跨学科研究领域,旨在探讨计算机科学与艺术的交汇点,让计算机得以像人类一样做出审美决策,包含美学的生成和度量两个方向,核心在于研究美的本质及如何利用计算机技术来实现和衡量美。计算美学的生成即利用计算机生成具备人类感知中美的特质的艺术作品,文章分析了当前美学生成研究核心的辅助生成、自动生成和风格迁移的现状与发展,讨论了美学生成的挑战与机遇。计算美学的度量即通过计算机技术实现对美的量化,文章从美学特征的视角介绍了美学度量的具体路径,以及生成式对抗网络、卷积神经网络等前沿的度量方法。

关键词：计算美学美学生成美学度量计算机艺术

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Rare loss-of-function variants in FLNB cause non-syndromic orofacial clefts

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Journal of Genetics and Genomics 2024年第2期51卷 222-229页

作者： Wenbin Huang Shiying Zhang Jiuxiang Lin Yi Ding nan jiang Jieni Zhang Huaxiang zhao Feng Chen Department of Orthodontics Peking University School and Hospital of StomatologyBeijing100081China Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research College of StomatologyXi’an Jiaotong UniversityXi’anShaanxi710004China Guangdong Provincial High-level Clinical Key Specialty Guangdong Province Engineering Research Center of Oral Disease Diagnosis and TreatmentDepartment of OrthodonticsStomatological CenterPeking University Shenzhen HospitalShenzhen Peking University-The Hong Kong University of Science and Technology Medical CenterShenzhenGuangdong 518036China Department of Physiology and Pathophysiology School of Basic Medical SciencesXi'an Jiaotong UniversityXi'anShaanxi710049China National Center for Stomatology National Clinical Research Center for Oral DiseasesNational Engineering Laboratory for Digital and Material Technology of StomatologyBeijing Key Laboratory for Digital StomatologyResearch Center of Engineering and Technology for Computerized Dentistry Ministry of HealthNMPA Key Laboratory for Dental MaterialsBeijing100101China Central Laboratory Peking University School and Hospital of StomatologyBeijing100081China

Orofacial clefts (OFCs) are the most common congenital craniofacial disorders, of which the etiology is closely related to rare coding variants. Filamin B (FLNB) is an actin-binding protein implicated in bone formation. FLNB mutations have been identified in several types of syndromic OFCs and previous studies suggest a role of FLNB in the onset of non-syndromic OFCs (NSOFCs). Here, we report two rare heterozygous variants (p.P441T and p.G565R) in FLNB in two unrelated hereditary families with NSOFCs. Bioinformatics analysis suggests that both variants may disrupt the function of FLNB. In mammalian cells, p.P441T and p.G565R variants are less potent to induce cell stretches than wild type FLNB, suggesting that they are loss-of-function mutations. Immunohistochemistry analysis demonstrates that FLNB is abundantly expressed during palatal development. Importantly, Flnb^(−/−) embryos display cleft palates and previously defined skeletal defects. Taken together, our findings reveal that FLNB is required for development of palates in mice and FLNB is a bona fide causal gene for NSOFCs in humans.

关键词： Or ofacial clefts FLNB Loss-of-function mutati on Cleft palate Filamin B Actin flannent Knockout mouse

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Left ventricular thrombosis caused cerebral embolism during venoarterial extracorporeal membrane oxygenation support: A case report

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World Journal of Clinical Cases 2024年第5期12卷 973-979页

作者： Yao-Bang Bai Feng zhao Zhen-Hua Wu Guo-Ning Shi nan jiang Intensive Care Unit Department of Cardiac SurgeryTianjin Chest HospitalTianjin 300222China Department of Cardiac Surgery Tianjin Chest HospitalTianjin 300222China

BACKGROUND Venoarterial(VA)extracorporeal membrane oxygenation(ECMO),an effective short-term circulatory support method for refractory cardiogenic shock,is widely applied.However,retrospective analyses have shown that VA-ECMO-assisted cases were associated with a relatively high mortality rate of approximately 60%.Embolization in important organs caused by complications of left ventricular thrombosis(LVT)during VA-ECMO is also an important reason.Although the incidence of LVT during VA-ECMO is not high,the consequences of embolization are disastrous.CASE SUMMARY A 37-year-old female patient was admitted to hospital because of fever for 4 d and palpitations for 3 d.After excluding the diagnosis of coronary heart disease,we established a diagnosis of“clinically explosive myocarditis”.The patient still had unstable hemodynamics after drug treatment supported by VA-ECMO,with heparin for anticoagulation.On day 4 of ECMO support,a left ventricular thro-mbus attached to the papillary muscle root of the mitral valve was found by transthoracic echocardiography.Left ventricular decompression was performed and ECMO was successfully removed,but the patient eventually died of multiple cerebral embolism.CONCLUSION LVT with high mobility during VA-ECMO may cause embolism in important organs.Therefore,a"wait and see"strategy should be avoided.

关键词： Venoarterial extracorporeal membrane oxygenation Left ventricular thrombosis Cerebral embolism Magnetic Resonance Imaging Therapy Case report

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Pollutant emissions from biomass burning:A review on emission characteristics,environmental impacts,and research perspectives

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Particuology 2024年第2期85卷 296-309页

作者： Ke jiang Ran Xing Zhihan Luo Wenxuan Huang Fan Yi Yatai Men nan zhao zhaofeng Chang jinfeng zhao Bo Pan Guofeng shen College of Urban and Environmental Sciences Peking UniversityBeijing100871China Beijing Key Lab Plant Resources Research and Development Beijing Technology and Business UniversityBeijing100048China College of Ecology and Environment Zhengzhou UniversityZhengzhou450001China Facultyof Environmental Sciences and Engineering Kunming University of Science and TechnologyYunnan Provincial Key Laboratoryof Soil Carbon Sequestration and Pollution ControlKunming650500China Institute of Carbon Neutrality Peking UniversityBeijing100871China

Biomass is one most abundant resource on the earth providing important energies in support of so-cioeconomic development in many areas.Burning of biomass fuels comprises to nearly 10%of the total energy from anthropogenic combustion processes:however,as the burning is usually incomplete,this process yields products of incomplete combustion posing consequently significant impacts on air quality,human health,and climate change.Here,we analyzed spatiotemporal characteristics in intentional and unintentional biomass burning from different sectors,discussed impacts of biomass burning emissions on indoor and outdoor air quality,and consequent influences on human health.The global total con-sumption amount of biomass including both natural and anthropogenic sources was approximately 7900 Tg in 2019,with significantly large regional and sectorial discrepancies among regions.Globally,anthropogenic biomass burning amounts increased gradually,but notably in some developing countries like China residential consumption of biomass fuels,as one large sector of biomass use,decreased over time.Uncommercial biomass consumption needs to be accurately quantified.There are relatively rich datasets of pollutant emission factors from biomass burning,including laboratory and field tests,but still large variations exit and contribute substantially to the uncertainty in emission inventory.Global pri-mary PM2.5,black carbon and organic carbon emissions from biomass burning were about 51,4.6,and 29 Tg,respectively,contributing to nearly 70%,55%,and 90%of the total emission from all sources,and emissions from the residential sector and open fires are major sources.Brown carbon emissions from biomass burning attracts growing interests but available studies adopted different methodologies challenging the comparability of those results.Biomass burning emissions polluted not only ambient air but more severely indoor air quality,adversely affecting human health.Future studies that should be emphasized and promoted are suggested.

关键词： Biomass burning Carbonaceous aerosol Emission Air quality Human health

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Unveiling the mechanisms of Fe(Ⅲ)-loaded chitosan composite(CTS-Fe)in enhancing anaerobic digestion of waste activated sludge

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Journal of Environmental Sciences 2024年第4期138卷 200-211页

作者： Boaiqi Zhang Ziwen zhao RuiMa nan Chen Zhe Kong Zhongfang Lei Zhenya Zhang Graduate School of Life and Environmental Sciences University of Tsukuba1-1-1 TennodaiTsukubaIbaraki 305-8572Japan The Key Laboratory of Water and Sediment Sciences Ministry of Education/State Environmental Protection Key Laboratory of All Material Fluxes in River Ecosystems/College of Environmental Sciences and EngineeringPeking UniversityBeijing 100871China South China Institute of Environmental Sciences Ministry of Ecology and EnvironmentGuangzhou 510345China School of Water Resources and Environment China University of Geosciences(Beijing)Beijing 100083China School of Environmental Science and Engineering Suzhou University of Science and TechnologySuzhou 215009China

Anaerobic digestion(AD)of waste activated sludge(WAS)is usually limited by the low generation efficiency of methane.Fe(Ⅲ)-loaded chitosan composite(CTS-Fe)have been reported to effectively enhanced the digestion of WAS,but its role in promoting anaerobic sludge digestion remains unclear.In present study,the effects of CTS-Fe on the hydrolysis and methanogenesis stages of WAS anaerobic digestion were investigated.The addition of CTSFe increased methane production potential by 8%-23%under the tested conditions with the addition of 5-20 g/L CTS-Fe.Besides,the results demonstrate that the addition of CTS-Fe could effectively promote the hydrolysis of WAS,evidenced by lower protein or polysaccharides concentration,higher soluble organic carbon in rector adding CTS-Fe,as well as the increased activity of extracellular hydrolase with higher CTS-Fe concentration.Meanwhile,the enrichment of Clostridia abundance(iron-reducing bacteria(IRBs))was observed in CTS-Fe adding reactor(8.9%-13.8%),which was higher than that in the control reactor(7.9%).The observation further suggesting the acceleration of hydrolysis through dissimilatory iron reduction(DIR)process,thus providing abundant substrates for methanogenesis.However,the presence of CTS-Fe was inhibited the acetoclastic and hydrogenotrophic methanogenesis process,which could be ascribed to the Fe(Ⅲ)act as electron acceptor coupled to methane for anaerobic oxidation.Furthermore,coenzyme F420 activity in the CTS-Fe added reactor was 34.9% lower than in the blank,also abundance of microorganisms involved in hydrogenotrophic methanogenesis was decreased.Results from this study could provide theoretical support for the practical applications of CTS-Fe.

关键词： Chitosan-Fe(Ⅲ)composite Methanogenesis Dissimilatory iron reduction Enzyme activity Microbial community structure

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Cellular atlases of ovarian microenvironment alterations by diet and genetically-induced obesity

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Science China(Life Sciences) 2024年第1期67卷 51-66页

作者： Yonghui jiang Xueying Gao Yue Liu Xueqi Yan Huangcong Shi Rusong zhao Zi-jiang Chen Fei Gao Han zhao Shigang zhao Center for Reproductive Medicine Shandong UniversityJinan 250012China Research Unit of Gametogenesis and Health of ART-Offspring Chinese Academy of Medical Sciences(No.2021RU001)Jinan 250012China Key Laboratory of Reproductive Endocrinology of Ministry of Education Shandong UniversityJinan 250012China Shandong Key Laboratory of Reproductive Medicine Shandong Provincial Hospital Affiliated to Shandong First Medical UniversityJinan 250012China Shandong Provincial Clinical Research Center for Reproductive Health Jinan 250012China National Research Center of Assisted Reproductive Technology and Reproductive Genetics Shandong UniversityJinan 250012China The Affiliated Suzhou Hospital of nanjing Medical University Suzhou Municipal HospitalGusu SchoolNanjing Medical UniversitySuzhou 215000China Center for reproductive medicine Ren Ji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200127China Shanghai Key Laboratory for Assisted Reproduction and Reproductive Genetics Shanghai 200127China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of ScienceBeijing 100101China

Obesity,which can arise from genetic or environmental factors,has been shown to cause serious damages to the reproductive system.The ovary,as one of the primary regulators of female fertility,is a complex organ comprised of heterogeneous cell types that work together to maintain a normal ovarian microenvironment(OME).Despite its importance,the effect of obesity on the entire ovary remains poorly documented.In this study,we performed ovary single-cell and nanoscale spatial RNA sequencing to investigate how the OME changed under different kinds of obesity,including high-fat diet(HFD)induced obesity and Leptin ablation induced obesity(OB).Our results demonstrate that OB,but not HFD,dramatically altered the proportion of ovarian granulosa cells,theca-interstitial cells,luteal cells,and endothelial cells.Furthermore,based on the spatial dynamics of follicular development,we defined four subpopulations of granulosa cell and found that obesity drastically disrupted the differentiation of mural granulosa cells from small to large antral follicles.Functionally,HFD enhanced follicle-stimulating hormone(FSH)sensitivity and hormone conversion,while OB caused decreased sensitivity,inadequate steroid hormone conversion,and impaired follicular development.These differences can be explained by the differential expression pattern of the transcription factor Foxo1.Overall,our study provides a powerful and high-resolution resource for profiling obesity-induced OME and offers insights into the diverse effects of obesity on female reproductive disorders.

关键词： obesity ovarian microenvironment snRNA-seq Stereo-seq Foxo1

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Effective assignment of positional isomers in dimeric shikonin and its analogs by ^(1)H NMR spectroscopy

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Chinese Chemical Letters 2024年第5期35卷 223-226页

作者： Ling-Hao zhao Hai-Wei Yan Jian-Shuang jiang Xu Zhang Xiang Yuan Ya-nan Yang Pei-Cheng Zhang State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China

An approach for distinguishing two types of positional isomers of dimeric shikonin and its analogs was explored with ^(4)JC,H long-range correlation by prolonging the acquisition time at ^(2,3)JC,H values of 2.0 and 8.0Hz.Furthermore,the ^(1)H(proton)nuclear magnetic resonance(NMR)pattern of phenolic hydroxyl protons was developed as a“diagnosis signal”to ascertain the relative location of each side chain in DMSO-d_(6) at sample concentrations of 0.022-0.034 mol/L.The chemical shift differences of 0.6ppm between OH-5' and OH-1 and between OH-8'and OH-4 are assigned to Type A and Type B,respectively.All reported ambiguous structures were corrected by this pattern.Additionally,the steric structures of isolated compounds were elucidated by quantum chemical calculations of electronic circular dichroism(ECD)spectra.

关键词： Arnebia euchroma Dimeric hydroxyl naphthoquinones Positional isomers ^(1)H NMR spectroscopy Chemical shift difference

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Artificial intelligence-assisted repair of peripheral nerve injury: a new research hotspot and associated challenges

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Neural Regeneration Research 2024年第3期19卷 663-670页

作者： Yang Guo Liying Sun Wenyao Zhong nan Zhang Zongxuan zhao Wen Tian Department of Hand Surgery Beijing Jishuitan HospitalBeijingChina

Artificial intelligence can be indirectly applied to the repair of peripheral nerve injury.Specifically,it can be used to analyze and process data regarding peripheral nerve injury and repair,while study findings on peripheral nerve injury and repair can provide valuable data to enrich artificial intelligence algorithms.To investigate advances in the use of artificial intelligence in the diagnosis,rehabilitation,and scientific examination of peripheral nerve injury,we used CiteSpace and VOSviewer software to analyze the relevant literature included in the Web of Science from 1994–2023.We identified the following research hotspots in peripheral nerve injury and repair:(1)diagnosis,classification,and prognostic assessment of peripheral nerve injury using neuroimaging and artificial intelligence techniques,such as corneal confocal microscopy and coherent anti-Stokes Raman spectroscopy;(2)motion control and rehabilitation following peripheral nerve injury using artificial neural networks and machine learning algorithms,such as wearable devices and assisted wheelchair systems;(3)improving the accuracy and effectiveness of peripheral nerve electrical stimulation therapy using artificial intelligence techniques combined with deep learning,such as implantable peripheral nerve interfaces;(4)the application of artificial intelligence technology to brain-machine interfaces for disabled patients and those with reduced mobility,enabling them to control devices such as networked hand prostheses;(5)artificial intelligence robots that can replace doctors in certain procedures during surgery or rehabilitation,thereby reducing surgical risk and complications,and facilitating postoperative recovery.Although artificial intelligence has shown many benefits and potential applications in peripheral nerve injury and repair,there are some limitations to this technology,such as the consequences of missing or imbalanced data,low data accuracy and reproducibility,and ethical issues(e.g.,privacy,data security,research transparency).Future re

关键词： artificial intelligence artificial prosthesis medical-industrial integration brain-machine interface deep learning machine learning networked hand prosthesis neural interface neural network neural regeneration peripheral nerve

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