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Aspirin inhibits tumor necrosis factor-a-stimulated fractalkine expression in human umbilical vein endothelial cells

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Chinese Medical Journal 2009年第10期122卷 1147-1153页

作者： jiang De-qian LIU Hong ZHANG She-bing ZHANG Xiao-lian Department of Cardiology Second Xiangya Hospital of Central Southern University Changsha Hunan 410011 China Department of Geriatrics Second Xiangya Hospital of Central Southern University Changsha Hunan 410011 China

Background Fractalkine is an important chemokine mediating local monocyte accumulation and inflammatory reactions in the vascular wall. Aspirin inhibits inflammatory cytokine expression closely related to atherosclerosis through the way independent of platelet and cyclooxygenase （COX）. There has been no report about the effect of aspirin on fractalkine expression. We aimed to determine the fractalkine expression in human umbilical vein endothelial cell （HUVEC） stimulated by tumor necrosis factor （TNF）-α and the effect of aspirin intervention. Methods Six of 8 HUVEC groups received either different concentrations of aspirin （0.02, 0.2, 1.0, 5.0 mmol/L） or 40 μmol/L pyrrolidinecarbodithioc acid （PDTC） or 0.5 μmol/L NS-398. The other two groups were negative control and positive control （TNF-α-stimulated）. After being incubated for 24 hours, cells of the 8 groups except the negative control one were stimulated with TNF-α （4 ng/ml） for another 24 hours. After that, the cells were collected for RNA isolation and protein extraction. Results Both mRNA and protein expressions of fractalkine in HUVEC were upregulated by 4 ng/ml TNF-α stimulation. Aspirin inhibited fractalkine expression in a dose-dependent manner at mRNA and protein levels. Nuclear factor-kappa B inhibitor, PDTC, effectively decreased the fractalkine expression. Fractalkine expression was not influenced by COX-2 selective inhibitor NS-398. COX-1 protein expression was not changed by either TNF-α stimulation or aspirin, PDTC, NS-398 intervention. Both mRNA and protein expression of COX-2 in HUVEC were upregulated by 4 ng/ml TNF-α stimulation. Aspirin decreased COX-2 expression in a dose-dependent manner at mRNA and protein levels. Conclusions TNF-α-stimulated fractalkine expression is suppressed by aspirin in a dose-dependent manner through the nuclear factor-kappa B p65 pathway.

关键词： aspirin fractalkine tumor necrosis factor-alpha nuclear factor-kappa B

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肿瘤静脉化疗安全用药规范的构建

中国护理管理 2024年第1期24卷 62-68页

作者：唐清姚丹王国蓉蒋倩罗蕾姚文秀张甜周红艳电子科技大学医学院护理系成都市610054 成都中医药大学护理学院四川省肿瘤医院护理部四川省肿瘤医院临床药学部四川省肿瘤医院肿瘤内科四川省肿瘤医院科技部

目的 :构建一套由多学科团队参与、涵盖化疗用药全环节的肿瘤静脉化疗安全用药规范,为化疗药物规范化使用提供参考。方法 :以物理-事理-人理系统方法论为指导,采用文献研究法形成规范初稿,通过专家会议对初稿进行讨论和修订后形成咨询稿... 详细信息

目的 :构建一套由多学科团队参与、涵盖化疗用药全环节的肿瘤静脉化疗安全用药规范,为化疗药物规范化使用提供参考。方法 :以物理-事理-人理系统方法论为指导,采用文献研究法形成规范初稿,通过专家会议对初稿进行讨论和修订后形成咨询稿,应用德尔菲专家咨询形成终稿。结果 :共对18名专家进行了3轮专家咨询,问卷回收率均为100%,专家权威系数分别为0.895、0.890、0.885,总条目的肯德尔协调系数为0.147~0.166(均P<0.001),三级条目的可操作性肯德尔协调系数为0.168~0.207(均P<0.001)。肿瘤静脉化疗安全用药规范包括4个一级条目(人员、环境、流程、监测与随访)、14个二级条目、47个三级条目,以及36个条目说明。结论:本研究构建的肿瘤静脉化疗安全用药规范纳入了“医-药-护”多学科专业规范要求,包含人员资质、用药环境、用药流程、监测与随访等全流程,有利于静脉化疗实践的多学科沟通合作,促进化疗用药过程的规范化和同质化,保障患者化疗用药安全。

关键词：物理-事理-人理系统方法论静脉化疗化疗用药用药规范化疗安全

A comprehensive review on natural phenolic compounds as alternatives to in-feed antibiotics

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Science China(Life Sciences) 2023年第7期66卷 1518-1534页

作者： Jingxia Gao Zhe Yang Chongqi Zhao Xiongzhuo Tang qian jiang Yulong Yin Animal Nutritional Genome and Germplasm Innovation Research Center College of Animal Science and TechnologyHunan Agricultural UniversityChangsha410128China Laboratory of Animal Nutritional Physiology and Metabolic Process Institute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China

Intensive livestock and poultry farming in China largely relied on the use of in-feed antibiotics until July *** consequences of antibiotic overuse in animal feed include accumulation in animal products and the development of bacterial antibiotic resistance,both of which threaten food safety and human *** has now completely banned the circulation of commercial feed containing growth-promoting drug additives(except Chinese herbal medicine).Therefore,alternatives to in-feed antibiotics in animal production are greatly *** phenolic compounds(NPCs)exist widely in plants and are non-toxic,nonpolluting,highly reproducible,and leave little *** natural flavonoids,phenolic acids,lignans,and stilbenes have polyphenol chemical structures and exhibit great potential as alternatives to *** this review we delineate the characteristics of plant-derived NPCs and summarize their current applications as alternatives to in-feed antibiotics,aiming to provide new strategies for antibiotic-free feeding and promote the development of more sustainable animal husbandry practices.

关键词： natural phenolic compounds alternatives to in-feed antibiotics animal husbandry intestinal health

基于JAK2/STAT3信号通路探讨黄芪-山茱萸对高糖诱导足细胞损伤的保护作用

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中国中医药科技 2024年第4期31卷 606-611页

作者：钱江王佳李志军李俊峰浙江医院浙江杭州310030 浙江中医药大学基础医学院浙江杭州310053

目的:探究黄芪-山茱萸对高糖诱导肾足细胞损伤的保护作用及机制。方法:CCK8法检测黄芪-山茱萸(0、175、350、700、1400、2800 mg/L)对足细胞(MPC-5)活性的影响。30 mmol/L葡萄糖诱导MPC-5细胞损伤,350、700、1400 mg/L黄芪-山茱萸进行干... 详细信息

目的:探究黄芪-山茱萸对高糖诱导肾足细胞损伤的保护作用及机制。方法:CCK8法检测黄芪-山茱萸(0、175、350、700、1400、2800 mg/L)对足细胞(MPC-5)活性的影响。30 mmol/L葡萄糖诱导MPC-5细胞损伤,350、700、1400 mg/L黄芪-山茱萸进行干预;ELISA测定MPC-5细胞IL-6、IL-1β水平,流式细胞术测定细胞凋亡,免疫荧光染色测定MPC-5肾病蛋白(nephrin)、足细胞素(podocin)表达,qRT-PCR测定MPC-5细胞nephrin、podocin、结蛋白(desmin)、Wilm瘤基因1(WT-1)基因表达,Western blot测定细胞Janus激酶2/信号转导和转录激活因子3(JAK2/STAT3)信号通路磷酸化表达。结果:2800 mg/L黄芪-山茱萸可显著抑制MPC-5细胞活性(P<0.01)。MPC-5细胞经高糖诱导后,IL-6、IL-1β水平升高,凋亡增加,nephrin、podocin、WT-1表达降低,desmin表达升高,JAK2/STAT3信号通路磷酸化表达增加(P<0.01)。350、700、1400 mg/L黄芪-山茱萸可抑制高糖诱导MPC-5细胞IL-6、IL-1β水平升高和凋亡,增强nephrin、podocin、WT-1表达,降低desmin表达,抑制JAK2/STAT3信号通路磷酸化表达(P<0.05)。结论:黄芪-山茱萸可减轻高糖诱导足细胞炎症和凋亡损伤,其机制与抑制JAK2/STAT3通路磷酸化有关。

关键词：黄芪-山茱萸足细胞凋亡 IL-6 IL-1β nephrin podocin desmin WT-1 JAK2/STAT3通路体外实验

Stable carbon isotopes in the shell of Corbicula fluminea (Müller 1774): Implications for understanding environmental changes in drainage basins

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Chinese Science Bulletin 2010年第36期55卷 4162-4167页

作者： ZHOU Hui LIU Congqiang jiang qian jiang Wei ZHAO YanLong DING WenCi YAN Hui State Key Laboratory of Environmental Geochemistry Institute of Geochemistry Chinese Academy of Sciences Guiyang 550002 China Graduate University of Chinese Academy of Sciences Beijing 100049 China

Dissolved inorganic carbon (DIC) is a primary constituent of the aquatic geochemistry of terrestrial ecosystems. Changes in DIC concentration and its isotopic composition are closely associated with environmental changes in the drainage basin. Thus, a better understanding of DIC may lead to improved characterization of environmental changes. Corbicula fluminea (Müller 1774), a bivalve native to southeast Asia and a dominant invasive species in many aquatic environments around the world, has the poten-tial to record DIC information, particularly because of its sequential skeletal deposition. Analysis of the stable carbon isotope composition (δ 13C) of the shell and the aquatic DIC in the Xijiang River drainage basins in southern and western China allowed us to determine that changes in δ 13C in the skeleton significantly correlated with those of DIC (P < 0.01, r = 0.63). These results indicate that the isotopic signature of the shell reflects that δ 13C of the DIC in ambient water. The temporal resolution was at the seasonal scale. Metabolic effect does not change the co-variation, even though it makes the shell δ 13C 1.4‰ lower statistically than that of DIC. δ 13C of DIC in creeks of some watershed areas may experience large temporal changes characterization of these changes requires high sampling resolution of the shell. This problem makes the clams in these areas less preferable for reconstruc-tion of environmental changes. The present findings provide the basis for recovering paleoenvironmental changes and carbon cycles in a watershed through analysis of fossil shells of C. fluminea, which are common in some lacustrine and/or riverine sedi-ment sequences.

关键词：稳定碳同位素组成古环境变化流域环境地球化学特征陆地生态系统外来入侵物种沉积序列 DIC

Visible Light and Temperature Regulated Reflection Colors in Self-supporting Cholesteric Liquid Crystal Physical Gels

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Chinese Journal of Polymer Science 2021年第12期39卷 1617-1625页

作者： Dong-Xu Zhao qian jiang Jing Wang Yuan Qiu Yong-Gui Liao Xiao-Lin Xie Key Laboratory of Material Chemistry for Energy Conversion and Storage Ministry of EducationHubei Engineering Research Center for Biomaterials and Medical Protective MaterialsHubei Key Laboratory of Materials Chemistry and Service FailureSchool of Chemistry and Chemical EngineeringHuazhong University of Science and TechnologyWuhan 430074China State Key Laboratory of Material Processing and Die&Mould Technology Huazhong University of Science and TechnologyWuhan 430074China

Photo-responsive cholesteric liquid crystals(CLCs)have attracted much attention in recent years due to their wide applications in filters,tun able optical lasers,dyn amic display devices,***,UV light is usually used as the exter nal stimulus source,which is not envir on ment-frie ndly *** the other hand,the mecha nical properties of CLCs are not strong eno ugh for these practical ***,it still remains a challenge to endow the CLCs with visible light response and high mechanical properties at the same ***,an axially chiral tetra-fluorinated binaphthyl azobenzene gelator(S-4F-AG)is *** 550 and 450 nm light irradiations,S-4F-AG exhibits excellent photo-switchable ***,the maximum con tent of c/s-isomer and its half-life are as high as 35%and 89 h in acet on itrile,respectively.A self-supporting CLC physical gel with a storage modulus around 104 Pa can be obtained when 3wt%S-4F-AG and 12wt%binaphthyl azobenzene derivative(dopant 2)are co-doped into a nematic LC host *** CLC physical gel exhibits a temperature-driven blue,green,and red reflection colors ***,such three primary RGB colors can also be realized by adjusting the exposure time of 550 nm green *** work lays a solid foundation for the applications ranging from information storage to high-tech anticounterfeit.

关键词： Cholesteric liquid crystal Gel Visible light response Reflect!on color Self-supporting ability

Robust Filtration Techniques in Geometrical Metrology and Their Comparison

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International Journal of Automation and computing 2013年第1期10卷 1-8页

作者： Shan Lou Wen-Han Zeng Xiang-qian jiang Paul J. Scott EPSRC Centre for Innovative Manufacturing in Advanced Metrology University of Huddersfield

Filtration is one of the core elements of analysis tools in geometrical metrology. Filtration techniques are progressing along with the advancement of manufacturing technology. Modern filtration techniques are required to be robust against outliers, applicable to surfaces with complex geometry and reliable in whole range of measurement data. A comparison study is conducted to evaluate commonly used robust filtration techniques in the field of geometrical metrology, including the two-stage Gaussian filter, the robust Gaussian regression filter, the robust spline filter and morphological filters. They are compared in terms of four aspects： functionality, mathematical computation, capability and characterization parameters. As a result, this study offers metrologists a guideline to choose the appropriate filter for various applications.

关键词： Geometrical metrology robust Gaussian regression filter robust spline filter morphological filters surface roughness

Rayleigh-Enhanced Nondegenerate Four-Wave Mixing

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Chinese Physics Letters 1996年第9期13卷 672-674页

作者： YU Zu-he MI Xin jiang qian Ll Xiao-feng FU Pan-ming Laboratory of Optical Physics Institute of PhysicsChinese Academy of SciencesBeijing 100080

We report a Rayleigh-enhanced nondegenerate four-wave mixing(NFWM)in carbon *** the Rayleigh-enhanced NFWM spectrum a relaxation time of as short as 220fs was deduced.

关键词： Rayleigh relaxation disulfide

Characterization of Binding Sites of Eukaryotic Transcription Factors

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Genomics, Proteomics & Bioinformatics 2006年第2期4卷 67-79页

作者： jiang qian Jimmy Lin Donald J. Zack The Wilmer Institute Department of Molecular Biology and Genetics Department of Neuroscience McKusick-Nathans Institute of Genetic Medicine Johns Hopkins University School of Medicine Baltimore MD 21287 USA.

To explore the nature of eukaryotic transcription factor （TF） binding sites and determine how they differ from surrounding DNA sequences, we examined four features associated with DNA binding sites： G＋C content, pattern complexity, palindromic structure, and Markov sequence ordering. Our analysis of the regulatory motifs obtained from the TRANSFAC databases using yeast intergenic sequences as background, revealed that these four features show variable enrichment in motif sequences. For example, motif sequences were more likely to have palindromic structure than were background sequences. In addition, these features were tightly localized to the regulatory motifs, indicating that they are a property of the motif sequences themselves and are not shared by the general promoter ＂environment＂ in which the regulatory motifs reside. By breaking down the motif sequences according to the TF classes to which they bind, more specific associations were identified. Finally, we found that some correlations, such as G＋C content enrichment, were species-specific, while others, such as complexity enrichment, were universal across the species examined. The quantitative analysis provided here should increase our understanding of protein-DNA interactions and also help facilitate the discovery of regulatory motifs through bioinformatics.

关键词： transcription factor promoter gene regulation bioinformatics