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A REF6-dependent H3K27me3-depleted state facilitates gene activation during germination in Arabidopsis

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Journal of Genetics and Genomics 2023年第3期50卷 178-191页

作者： Jie Pan Huairen Zhang Zhenping Zhan Ting Zhao danhua jiang State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Seed germination is a critical developmental switch from a quiescent state to active growth,which involves extensive changes in metabolism,gene expression,and cellular ***,our understanding of epigenetic and transcriptional reprogramming during this process is *** histone H3 lysine 27 trimethylation(H3K27me3)plays a key role in regulating gene repression and cell fate ***,we profile H3K27me3 dynamics and dissect the function of H3K27 demethylation during germination in *** temporal genome-wide profiling of H3K27me3 and transcription reveals delayed H3K27me3reprogramming compared with transcriptomic changes during germination,with H3K27me3 changes mainly occurring when the embryo is entering into vegetative *** OF EARLY FLOWERING 6(REF6)-mediated H3K27 demethylation is necessary for robust germination but does not significantly contribute to H3K27me3 dynamics during germination,but rather stably establishes an H3K27me3-depleted state that facilitates the activation of hormone-related and expansin-coding genes important for *** also show that the REF6 chromatin occupancy is gradually established during germination to counteract increased Polycomb repressive complex 2(PRC2).Our study provides key insights into the H3K27me3 dynamics during germination and suggests the function of H3K27me3 in facilitating cell fate ***,we reveal the importance of H3K27 demethylation-established transcriptional competence in gene activation during germination and likely other developmental processes.

关键词： H3K27me3 Epigenetic reprogramming H3K27 demethylation REF6 Germination Arabidopsis thaliana

The INO80 chromatin remodeling complex promotes thermo-morphogenesis by connecting H2A.Z eviction and active transcription in Arabidopsis.

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Molecular Plant 2021年第11期14卷 1799-1813页

作者： Xue Mande Zhang Huairen Zhao Fengyue Zhao Ting Li Hui jiang danhua State Key Laboratory of Plant Genomics Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina

Global warming imposes a major threat to plant growth and crop production. In some plants including Arabidopsis thaliana, elevated temperatures induce a series of morphological and developmental adjustments, termed thermomorphogenesis to facilitate plant cooling under high-temperature conditions. Plant thermal response is suppressed by histone variant H2A.Z. At warm temperatures, H2A.Z is evicted from nucleosomes at thermo-responsive genes, resulting in their expression changes. However, the mechanisms that regulate H2A.Z eviction and subsequent transcription changes are largely unknown. Here, we show that the INO80 chromatin remodeling complex (INO80-C) promotes thermomorphogenesis and activates the expression of thermo-responsive and auxin-related genes. INO80-C associates with PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), a potent regulator in thermomorphogenesis, and mediates temperature-induced H2A.Z eviction at PIF4 targets. Moreover, INO80-C directly interacts with COMPASS-like and transcription elongation factors to promote active histone modification Histone H3 lysine 4 trimethylation (H3K4me3) and RNA Polymerase II (RNA Pol II) elongation, leading to the thermal induction of transcription. Notably, transcription elongation factors SPT4 and SPT5 are required for the H2A.Z eviction at PIF4 targets, suggesting the cooperation of INO80-C and transcription elongation in H2A.Z removal. Our results demonstrate that the (PIF4)-(INO80-C)-(COMPASS-like)-(transcription elongator) module controls plant thermal response, and establish a link between H2A.Z eviction and active transcription.

关键词： Arabidopsis H2A.Z INO80 PIF4 thermomorphogenesis transcription elongation

Spatial dynamics of a nonlocal delayed unstirred chemostat model with periodic input

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International Journal of Biomathematics 2019年第6期12卷 55-72页

作者： Wu-Jun Pu danhua jiang Ya Wang Zhanbing Bai Department of Mathematics Longnan Teachers College LongnanGansu 742500P.R.China School of Mathematics and Statistics Lanzhou University LanzhouGansu 730000P.R.China College of Mathematics and System Science Shandong University of Science and Technology Qingdao Shandong 266590P.R.China

In this paper,a nonlocal delayed chernostat model of a single species feeding on a periodically varying input nutrient is *** the theory of semigroup,the existence and uniqueness of solution of the system are ***,we i... 详细信息

关键词： Chernostat nonlocal delay uniform persistence periodic solution

A warm temperature-released negative feedback loop fine-tunes PIF4-mediated thermomorphogenesis in Arabidopsis

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Plant Communications 2024年第5期5卷 208-224页

作者： Hui Li Mande Xue Huairen Zhang Fengyue Zhao Xiaoyi Li Shuancang Yu danhua jiang Key Laboratory of Seed Innovation Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina University of Chinese Academy of Sciences BeijingChina Beijing Vegetable Research Center Beijing Academy of Agriculture and Forestry Sciences(BAAFS)BeijingChina National Center for Soybean Improvement State Key Laboratory of Crop Genetics and Germplasm EnhancementCollege of AgricultureNanjing Agricultural UniversityNanjingChina

Plants can sense temperature changes and adjust their growth *** Arabidopsis,high ambient temperatures stimulate stem elongation by activating a key thermoresponsive regulator,PHYTOCHROME INTERACTING FACTOR 4(PIF4).Here,we show that warmth promotes the nighttime transcription of GI,which is necessary for the high temperature-induced transcription of *** analyses suggest that GI prevents excessive thermoresponsive growth by inhibiting PIF4,with this regulatory mechanism be-ing partially reliant on *** transcription is repressed by ELF3 and HY5,which concurrently inhibit PIF4 expression and *** elevation causes the deactivation or degradation of ELF3 and HY5,leading to PIF4 activation and relief of GI transcriptional repression at high *** allows PIF4 to further activate GI transcription in response to elevated ***,in turn,inhibits PIF4,es-tablishing a negative feedback loop thatfine-tunes PIF4 *** addition,we demonstrate that ELF3,HY5,and PIF4 regulate GI transcription by modulating the enrichment of histone variant H2A.Z at the GI ***,ourfindings suggest that thermal release of a negative feedback loopfinely adjusts plant thermomorphogenesis.

关键词： temperature thermomorphogenesis PIF4 GI TOC1 feedback loop

LHP1 Interacts with ATRX through Plant-Specific Domains at Specific Loci Targeted by PRC2

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Molecular Plant 2018年第8期11卷 1038-1052页

作者： Haifeng Wang danhua jiang Elin Axelsson Zdravko J. Lorkovic Sean Montgomery Sarah Holec Bas J.G.E. Pieters Abbas H.K. AI Temimi Jasmin Mecinovic Frederic Berger Gregor Mendel Institute (GMI) Austrian Academy of Sciences Vienna Biccenter (VBC) Dr. Bohr-Gasse 3 1030 Vienna Austria Temasek Lifesciences Laboratory 1 Research Link Nationat University of Singapore 117604 Singapore Singapore Department of Biological Sciences National University of Singapore 14 Science Drive 4 117543 Singapore Singapore Institute for Molecules and Materials Radboud University Heyendaalseweg 135 6525 AJ Nijmegen the Netherlands

Heterochromatin Protein 1 （HP1） is a major regulator of chromatin structure and function. In animals, the network of proteins interacting with HP1 is mainly associated with constitutive heterochromatin marked by H3K9me3. HP1 physically interacts with the putative ortholog of the SNF2 chromatin remodeler ATRX, which controls deposition of histone variant H3.3 in mammals. In this study, we show that the Arabidopsis thaliana ortholog of ATRX participates in H3.3 deposition and possesses specific conserved domains in plants. We found that plant Like HP1 （LHP1） protein interacts with ATRX through domains that evolved specifically in land plant ancestors. Loss of ATRX function in Arabidopsis affects the expression of a limited subset of genes controlled by PRC2 （POLYCOMB REPRESSIVE COMPLEX 2）, including the flowering time regulator FLC. The function of ATRX in regulation of flowering time requires novel LHPl-interacting domain and ATPase activity of the ATRX SNF2 helicase domain. Taken together, these results suggest that distinct evolutionary pathways led to the interaction between ATRX and HP1 in mammals and its counterpart LHP1 in plants, resulting in distinct modes of transcriptional regulation.

关键词： ATRX LHPI1 H3 variants Chromatin Histone Evolution

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大豆蛋白/PTT纤维混纺纱性能研究

上海纺织科技 2016年第8期44卷 18-20,37页

作者：须峰姜丹华上海怀英贸易有限公司上海200000 东华大学上海201620

大豆蛋白纤维与PTT纤维在性能上各有优缺点,采用环锭纺和赛络纺两种纺纱方法,将大豆纤维、PTT纤维进行混纺,可以利用两种纤维各自的特点,达到优势互补的效果。对大豆蛋白/PTT纤维混纺纱线的毛羽、强伸度和条干等指标进行了对比分析。结... 详细信息

大豆蛋白纤维与PTT纤维在性能上各有优缺点,采用环锭纺和赛络纺两种纺纱方法,将大豆纤维、PTT纤维进行混纺,可以利用两种纤维各自的特点,达到优势互补的效果。对大豆蛋白/PTT纤维混纺纱线的毛羽、强伸度和条干等指标进行了对比分析。结果表明:随着PTT纤维含量的增加,纱线强度有所降低,毛羽显著改善,条干均匀度有所下降;赛络纺纱线在强力和毛羽上均优于环锭纺纱线。

关键词：大豆蛋白纤维 PTT 混纺比环锭纺赛络纺

C反应蛋白、PCT联合中性粒细胞淋巴细胞比值在肺结核患者中的辅助诊断效能及与血培养结果的关系

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中国医药指南 2023年第23期21卷 49-52页

作者：姜丹花漳平市医院检验科福建漳平364400

目的探讨C反应蛋白(CRP)、降钙素原(PCT)联合中性粒细胞淋巴细胞比值(NLR)在肺结核患者中的辅助诊断效能及与血培养结果的关系。方法选取2020年6月至2021年6月我院收治的肺结核患者68例为研究组(活动组39例,非活动组29例),另选取来我... 详细信息

目的探讨C反应蛋白(CRP)、降钙素原(PCT)联合中性粒细胞淋巴细胞比值(NLR)在肺结核患者中的辅助诊断效能及与血培养结果的关系。方法选取2020年6月至2021年6月我院收治的肺结核患者68例为研究组(活动组39例,非活动组29例),另选取来我院体检的同期健康对照组68名,对两组纳入研究对象的CRP、PCT、NLR水平进行检测和分析,同时对比肺结核不同病情间的CRP、PCT、NLR水平,分析与血培养结果间的关系。结果研究组CRP、PCT、NLR水平高于对照组(P <0.05);在研究组中,活动组患者CRP、PCT、NLR水平高于非活动组(P <0.05);CRP、PCT、NLR水平与血培养结果有着密切的关系;CRP、PCT、NLR联合检测诊断效能优于各项指标单一检测(P <0.05);CRP、PCT、NLR水平联合检测的满意度高于单一指标检测(P <0.05)。结论 C反应蛋白、PCT联合NLR在肺结核患者中有着较好的诊断效果,可反映肺结核患者的病情程度。

关键词：肺结核 C反应蛋白降钙素原中性粒细胞淋巴细胞比值诊断效能血培养

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高频肠道超声评估新生儿坏死性小肠结肠炎

中国医学影像技术 2023年第5期39卷 696-699页

作者：蒋丹华欧作强刘萍萍黎新艳广西壮族自治区妇幼保健院超声医学科广西南宁530003

目的探讨高频肠道超声评估新生儿坏死性小肠结肠炎(NEC)的价值。方法纳入304例新生儿NEC,根据临床转归将其分为转归不良组(n=64,接受急性期手术治疗)及转归良好组(n=240,接受急性期保守治疗)。比较组间高频肠道超声表现的差异,应用log... 详细信息

目的探讨高频肠道超声评估新生儿坏死性小肠结肠炎(NEC)的价值。方法纳入304例新生儿NEC,根据临床转归将其分为转归不良组(n=64,接受急性期手术治疗)及转归良好组(n=240,接受急性期保守治疗)。比较组间高频肠道超声表现的差异,应用logistic回归分析筛选预测新生儿NEC转归不良的独立因素;绘制受试者工作特征曲线,评估肠道超声征象预测新生儿NEC转归不良的价值。结果转归不良组64例术中均见肠管坏死或穿孔;转归良好组经内科保守治疗后好转。转归不良组患儿腹腔游离气体、腹腔积液、门静脉积气、肠壁积气、肠壁增厚、肠管扩张及肠梗阻超声检出率均明显高于转归良好组(P均18.1 mm是新生儿NEC临床转归不良的独立预测因素(P均<0.05),其联合预测新生儿NEC转归不良的曲线下面积为0.75,敏感度为93.66%,特异度为82.32%。结论高频肠道超声可用于评估新生儿NEC。

关键词：婴儿,新生儿小肠结肠炎,坏死性肠穿孔超声检查

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植物基因组与表观遗传学研究进展

植物生理学报 2023年第9期59卷 1665-1693页

作者：肖军鲁非邓娴宋显伟贺飞蒋才富刘倩赵玉胜姜丹华傅向东中国科学院遗传与发育生物学研究所植物细胞与染色体工程国家重点实验室北京100101 中国科学院遗传与发育生物学研究所植物基因组学国家重点实验室北京100101 中国农业大学生物学院植物抗逆高效全国重点实验室北京100193

DNA序列信息是基因功能研究的基础,近10年来随着测序技术的升级迭代,植物基因组学高速发展。不同倍性植物高质量参考基因组的解析,重要作物泛基因组的构建,以及大规模、群体水平的深度重测序等极大推动了作物起源、驯化、农艺性状形成... 详细信息

DNA序列信息是基因功能研究的基础,近10年来随着测序技术的升级迭代,植物基因组学高速发展。不同倍性植物高质量参考基因组的解析,重要作物泛基因组的构建,以及大规模、群体水平的深度重测序等极大推动了作物起源、驯化、农艺性状形成的解析以及育种改良的进程。表观遗传修饰参与植物生长发育的全过程,调控了植物对环境信号如光、温度、养分、盐碱等的应答和适应性,介导了同代和代际之间的“胁迫记忆”。除了DNA甲基化、小分子RNA调控、组蛋白修饰,近几年RNA结构与修饰以及染色质的三维结构也得到越来越多的关注,逐渐成为表观遗传研究的热点。本文主要概述了近10年来植物基因组和表观遗传学研究的前沿进展,比较国内外研究的侧重点,并探讨后续的发展趋势,提出未来5~10年的研究目标和项目建议。

关键词：参考基因组泛基因组起源与驯化 DNA甲基化组蛋白修饰染色质三维结构 RNA修饰小分子RNA