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Expression of iNOS in early injury in a rat model of small-for-size liver transplantation

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Hepatobiliary & Pancreatic Diseases International 2009年第2期8卷 146-151页

作者： jiang, wei-wei Kong, Lian-Bao Li, Guo-Qiang Wang, Xue-Hao Nanjing Med Univ Affiliated Hosp 1 Liver Transplantat Ctr Natl Inst Living Donor Liver Transplantat Nanjing 210029 Peoples R China

BACKGROUND: Living donor liver transplantation has been widely accepted as the treatment of choice for end-stage liver disease. Large amounts of nitric oxide generated by inducible nitric oxide synthase (iNOS) have been shown to play an important role in many inflammatory and immune reactions, but expression of iNOS in small-for-size liver transplantation is unknown. The aims of this study were to determine the time course of iNOS mRNA and protein as well as the redox state of liver biopsies in a rat model of small-for-size liver transplantation. METHODS: Male Sprague-Dawley rats were divided into a control group, a warm ischemia-reperfusion (IR) group, and a small-for-size liver graft group. Real-time RT-PCR and Western blotting were used to characterize the time course of the expression of iNOS mRNA and protein, respectively. Malondialdehyde (MDA) and superoxide dismutase (SOD) were used as markers to characterize the redox state of liver tissues, and the time courses of MDA and SOD levels were also measured. RESULTS: The expression of iNOS mRNA and protein levels in the warm IR and small-for-size graft groups both significantly increased after reperfusion, and peaked at 3 hours. Moreover, the increase in MDA was accompanied by increased iNOS in the period of 1-24 hours after reperfusion. The MDA levels in the warm IR and small-for-size graft groups significantly increased after reperfusion, peaked at 3 hours, and decreased thereafter. The direction of change in SOD was opposite that of the change in MDA. CONCLUSIONS: The expression of iNOS mRNA and protein is activated after reperfusion both in hepatic warm IR injury and small-for-size liver graft. Furthermore, the results of this study suggest that iNOS contributes to the damage in warm IR injury and small-for-size grafts via free oxygen radicals.

关键词： inducible nitric oxide synthase small-for-size graft ischemia-reperfusion injury liver transplantation

Multi-Beam Interference Transmission Spectrum Observed from an Eccentric Core Single-Mode Fiber

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Chinese Physics Letters 2011年第2期28卷 122-125页

作者： FAN Lin-Yong jiang wei-wei ZHAO Rui-Feng PEI Li JIAN Shui-Sheng Institute of Lightwave Technology Beijing Jiaotong UniversityBeijing 100044 Key Lab of All Optical Network and Advanced Telecommunication Network of EMC Beijing Jiaotong UniversityBeijing 100044

An eccentric core single-mode fiber,whose core is away from the axis of the fiber,is fabricated by using the fiber preform goniometric-groove method and the stack-and-draw *** eccentric core single-mode fiber without coating is spliced between two single-mode *** mode and cladding modes are excited at one splicing point between the eccentric core single-mode fiber and the single-mode fiber,and propagate in the eccentric core single-mode fiber,then interfere with each other at the other splicing ***-beam interference transmission spectrum is *** in-line fiber interferometric strain sensor based on the eccentric core single-mode fiber is realized.

关键词： fiber mode cladding

Studies on the Fluorescence Enhancement Effect of Eu-La-DBM-DPG-Acetone System

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Chemical Research in Chinese Universities 1992年第1期8卷 41-44页

作者： Zhu Guiyun, Si Zhikun, jiang wei and Li wei (Department of Applied Chemistry, Shandong University, Jinan)

The study of fluorescence enhancement has attracted attention recently. For example, the sensitivity and selectivity of Eu-thenoyltrifluoroacetone （TTA）phenan-throline and Eu-diphacinone-ammonia systems in Triton X-... 详细信息

关键词： Europium Lanthanum Fluorescence enhancement

Elastic fiber regeneration in vitro and in vivo for treatment of experimental abdominal aortic aneurysm

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Chinese Medical Journal 2013年第3期126卷 437-441页

作者： XIONG jiang GUO wei wei Ren ZUO Shang-wei LIU Xiao-ping ZHANG Tao Department of Vascular and Endovascular Surgery Vascular Surgery Center of People's Liberation Army Chinese People's Liberation Army General Hospital Beijing 100853 China

Background The pathological characteristics of abdominal aortic aneurysm （AAA） involved the regression of extracellular matrix （ECM） in aortic walls, especially elastic structure in medial layer. As the major structural protein of aorta, elastin contributes to the extensibility and elastic recoil of the vessels. We hypothesized that overexpression of elastin in vessel walls might regenerate the elastic structure of ECM, restore the elastic structure of the aneurysmal wall, and eventually lead to a reduction of aortic diameters （ADs） in an experimental model of AAA. Methods Tropoelastin （TE） of Sprague Dawley （SD） rat was synthesized by reverse transcription polymerase chain reaction and used to construct adneviral vectors containing elastin precursor protein （AdTE-GFP）. Cultured vascular smooth muscle cells （VSMCs） from aortas of male SD rats were transfected with AdTE-GFP, AdGFP, adenoviral vector （AdNull）, and phosphate buffered saline （PBS）. Immunofluorescence staining was performed to determine the expression of elastin in transfected cells. The expression of elastic fibers in ECM of VSMCs transfected with AdTE-GFP were detected by fluorescence microscopy and transmission electron microscopy （TEM） at 1, 3, and 5 days following gene transfer. The AAA vessel walls were infused with AdTE-GFP or an empty AdNull, or PBS directly into the aneurysmal lumen. ADs of the aneurysms were compared in infused aortas. Formation of new elastic fibers in vivo was assessed by hematoxylin and eosin, and elastic von-Giesson staining. Recombinant elastin-GFP in vivo was identified by immunohistochemical staining. Results Elastic fibers were increased both in ECM of VSMC and in vessel walls after gene transfer. Histological studies revealed that the AdTE-GFP-transduced aortas had elastic fiber regeneration in the aneurysmal walls. The AdTE-GFP-transduced aortas showed a decreased AD （23.04%±14.49%, P 〈0.01） in AAA vessel walls. Conclusions Elastic fibers have been successfully overex

关键词： abdominal aortic aneurysm elastic fiber adenovirus animal model vascular smooth muscle cell ultr astructure

Biomechanical comparison of cervical transfacet pedicle screws versus pedicle screws

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Chinese Medical Journal 2008年第15期121卷 1390-1393页

作者： LIU Guan-yi XU Rong-ming MA wei-hu SUN Shao-hua HUANG Lei YING jiang-wei jiang wei-yu Department of Orthopaedics Ningbo Sixth People's Hospital Ningbo Zhejiang 315040 China

Background Transfacet pedicle screws provide another alternative for standard pedicle screw placement for plate fixation in the lumbar spine. However, few studies looking at transfacet pedicle screw fixation in the cervical spine are available. Therefore, cervical transfacet pedicle screw fixation and standard pedicle screw fixation techniques were biomechanically compared in this study. Methods Ten fresh human cadaveric cervical spines were harvested. On one side, transfacet pedicle screws were placed at the C3-4, C5-6, and C7-T1 levels. On the other side, pedicle screws were placed at the C3, C5, and C7 levels. The screw insertion technique at each level was randomized for right or left. The starting point for the transfacet pedicle screw insertion was located at the midpoint of the inferolateral quadrant of the lateral mass and the direction of the screw was about 50° caudally in the sagittal plane and about 45° toward the midline in the axial plane. Screws were placed from the inferior articular process, across the facet complex and the pedicle into the body of the caudal vertebra. The entry point for the pedicle screw was located at the midpoint of the superolateral quadrant of the lateral mass, and the direction of the screw was about 45° toward the midline in the axial plane and toward the upper third of the vertebral body in the sagittal plane. After screw placement we performed axial pullout testing. Results All the cervical transfacet pedicle screws and the pedicle screws were inserted successfully. The mean pullout strength for the transfacet pedicle screws was 694 N, while for the pedicle screws 670 N （P=0.013）. In all but six instances （10%）, the pedicle screw pullout values exceeded the values for the transfacet pedicle screws; this occurred three times at the C3/C4 level, twice at the C5/C6 level and once at the C7/T1 level. The greatest pullout strength difference at a single level was observed at the C5/C6 level, with a mean difference of 38 N （t=-1

关键词： cervical spine fixation transfacet pedicle screws pedicle screws

Coronal Magnetic Flux Rope Equilibria and Magnetic Helicity

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Chinese Journal of Astronomy and Astrophysics 2001年第1期1卷 77-84页

作者： You－QinHu Yan－weijiang 等 DepartmentofEarthandSpaceScience UniversityofScienceandTechnologyofChinaHefei230026 DepartmentofEart

Using a 2.5-dimensional (2.5-D) ideal MHD model, this paper analyzes the equilibrium properties of coronal magnetic flux ropes in a bipolar ambient magnetic field. It is found that the geometrical features of the magnetic flux rope, including the height of the rope axis, the half-width of the ropes and the length of the vertical current sheet below the ropes are determined by a single magnetic parameter, the magnetic helicity, which is the sum of the self-helicity of the rope and the mutual helicity between the rope field and the ambient magnetic field. All the geometrical parameters increase monotonically with increasing magnetic helicity. The implication of this result in solar active phenomena is briefly discussed.

关键词： Sun: magnetic fields - Sun: magnetic flux rope - Sun: magnetic helicity

Role and mechanism of matrine alone and combined with acitretin for HaCaT cells and psoriasis-like murine models

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Chinese Medical Journal 2019年第17期132卷 2079-2088页

作者： wei-wei jiang Yi-Meng Wang Xiao-Yu Wang Qian Zhang Si-Man Zhu Chun-Lei Zhang Department of Dermatology Peking University Third Hospital Beijing 100191 China

Background: Acitretin and matrine have been used in the treatment of psoriasis in China. This study was designed to investigate the role and related mechanisms of matrine alone and in combination with acitretin in the treatment of psoriasis in vitro and in vivo. Methods: HaCaT cells were treated with matrine at different concentrations of 0 (blank control), 0.2, 0.4, 0.8, and 1.6 mg/mL for 24, 48, 72 h, respectively. 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium cell viability assay was used to assess the growth and proliferation of HaCaT cells. Cell cycle and apoptosis were detected by flow cytometry. Expression of protein was detected by Western blotting. Autophagy was observed by transmission electron microscopy. Then HaCaT cells were assigned to normal saline (NS) control group, matrine (0.4 mg/mL) group, acitretin (10 μmol/L) group, and matrine plus acitretin group, and the above methods were repeated. In animal experiments, the cumulative score (erythema, scaling, thickening) as a measure of the severity of inflammation was used to measure the skin performance of mice after treated with matrine 50 mg/kg, acitretin 4.5 mg/kg or combination of the two drugs on the psoriasis-like mouse models, respectively. Pathological findings of the lesions were observed, and the protein expressions in the lesions were detected by immunohistochemistry. Results: Cell proliferation inhibition was seen in HaCaT cells with treatment of matrine in a dose- and time-dependent manner (P < 0.01, respectively). Cell cycle G0/G1 phase arrest was observed in a dose-dependent way (P < 0.01). The expression of p21 (P < 0.05), LC3II/I (P < 0.01), and Beclin 1 (P < 0.01) increased and the expression of cyclin D1 (P < 0.05) decreased with increasing doses of matrine. Compared with the blank control, more autophagosomes were seen in HaCaT cells treated with matrine at 0.4 mg/mL by transmission electron microscopy (2.667 ± 1.202 vs. 21.33 ± 1.453, t = 9

关键词： Psoriasis Autophagy Phosphoinositide 3-kinase Protein kinase B Mammalian target of rapamycin

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自由曲面镜片波前像差扩束-缩束拼接技术

光子学报 2019年第8期48卷 154-163页

作者：禹静蒋威炜沈小燕中国计量大学计量测试工程学院

为解决波前传感器检测自由曲面镜片时测量口径不匹配,在拼接检测方法中测量过程及数据处理较为繁琐等问题,提出了一种测量自由曲面镜片的方法.该方法在扩大单次测量光束直径后对自由曲面镜片波前像差进行检测,再由波前传感器接收缩小后... 详细信息

为解决波前传感器检测自由曲面镜片时测量口径不匹配,在拼接检测方法中测量过程及数据处理较为繁琐等问题,提出了一种测量自由曲面镜片的方法.该方法在扩大单次测量光束直径后对自由曲面镜片波前像差进行检测,再由波前传感器接收缩小后的带有扩束测量区域波前信息的光束.设计光路测量得到4个直径扩大10倍后的子孔径波前像差,完成扩束子孔径的波前重构及拼接.为验证实验的可靠性,对测得的扩束子孔径进行波前重构后与哈特曼传感器生成的子孔径像差峰谷值进行比较,并观察扩束子孔径拼接波面的平整性.实验结果表明:该方法能有效增大小口径波前传感器的测量范围,提高检测效率,可以用于大口径镜片的波前像差检测上.

关键词：自由曲面镜片扩束缩束拼接法波前像差波前重构像差峰谷值

Catheter ablation of atrial fibrillation in a patient with dextrocardia： what is the challenge？

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Chinese Medical Journal 2010年第17期123卷 2478-2479页

作者： WANG Xin-hua SHI Hai-feng HAN Bing TAN Hong-wei jiang wei-feng LIU Xu Department of Cardiology Shanghai Chest Hospital Affiliated to Shanghai Jiao Tong University Shanghai 200030 China

Catheter ablation has been an established strategy for treating paroxysmal atrial fibrillation （AF）.Pulmonaryvein isolation is the predominant approach of catheter ablation. This procedure is characterized as transseptal catheterization and point-by-point ablation around the ipsilateral pulmonary veins （PVs）. Although catheter ablation can be safely performed in a heart with normal structures, it may be challenging to be performed in a dextrocardia.

关键词：导管消融术肺静脉心房颤动心脏血气分析胰岛素右位心铍