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Maternal Thermal Effects on Female Reproduction and Hatchling Phenotype in the Chinese Skink(Plestiodon chinensis)

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Asian Herpetological Research 2018年第4期9卷 250-257页

作者： Li MA Zhihua LIN jianfang GAO Hong LI Xiang JI Hongliang lu Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life Sciences Nanjing Normal University School of Ecology Lishui University Hangzhou Key Laboratory for Animal Adaptation and Evolution School of Life and Environmental Sciences Hangzhou Normal University

We maintained gravid Chinese skinks(Plestiodon chinensis) at three constant temperatures(25, 28 and 31 °C) during gestation, and randomly assigned eggs from each female to one of the same three temperatures for incubation to determine maternal thermal effects on female reproduction and hatchling phenotype. Maternal temperature affected egg-laying date, hatching success and hatchling linear size(snout-vent length, SVL) but not clutch size, egg size, egg component, and embryonic stage at laying. More specifically, females at higher temperatures laid eggs earlier than did those at low temperatures, eggs laid at 31 °C were less likely to hatch than those laid at 25 °C or 28 °C, and hatchlings from eggs laid at 31 °C were smaller in SVL. Our finding that maternal temperature(pre-ovipositional thermal condition) rather than incubation temperature(post-ovipositional thermal condition) affected hatching success indicated that embryos at early stages were more vulnerable to temperature than those at late stages. Our data provide an inference that moderate maternal temperatures enhance reproductive fitness in P. chinensis.

关键词： Developmental plasticity egg incubation female reproduction hatchling phenotype maternal thermal effect Plestiodon chinensis

Oceanic environmental changes of subarctic Bering Sea in recent 100 years: Evidence from molecular fossils

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Science China Earth Sciences 2005年第4期48卷 555-564页

作者： lu Bing CHEN Ronghua ZHOU Huaiyang WANG Zipan CHEN jianfang ZHU Chun Key Laboratory of State Oceanic Administration The Second Institute of Oceanography Hangzhou 310012 China Key Laboratory of State Oceanic Administration The Second Institute of Oceanography Hangzhou 310012 China Key Laboratory of State Oceanic Administration The Second Institute of Oceanography Hangzhou 310012 China Key Laboratory of State Oceanic Administration The Second Institute of Oceanography Hangzhou 310012 China Key Laboratory of State Oceanic Administration The Second Institute of Oceanography Hangzhou 310012 China Key Laboratory of State Oceanic Administration The Second Institute of Oceanography Hangzhou 310012 China

The core sample B2-9 from the seafloor of the subarctic Bering Sea was dated with 210 Pb to obtain a consecutive sequence of oceanic sedimentary environments at an interval of a decade during 1890—1999. A variety of molecular fossils were detected, including n-alkanes, isoprenoids, fatty acids, sterols, etc. By the characteristics of these fine molecules (C27, C28, and C29 sterols) and their molecular indices (Pr/Ph, ∑C+22/∑C?21, CPI and C18∶2/C18∶0) and in con- sideration of the variation of organic carbon content, the 100-year evolution history of subarctic sea paleoenvironment was reestablished. It is indicated that during the past 100 years in the Arctic, there were two events of strong climate warming (1920—1950 and 1980—1999), which resulted in an oxidated sediment environment owing to decreasing terrigenous organic matters and increasing marine-derived organic matters, and two events of transitory climate cooling (1910 and 1970—1980), which resulted in a slightly reduced sediment environment owing to increasing terrigenous organic matters and decreasing marine-derived organic matters. It is re- vealed that the processes of warming/cooling alternated climate are directly related to the Arctic and global climate variations.

关键词： subarctic, molecular fossil, paleoenvironment, record of stratigraphy.

Genetic and antigenic characterization of recombinant nucleocapsid proteins derived from canine coronavirus and canine respiratory coronavirus in China

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Science China(Life Sciences) 2016年第6期59卷 615-621页

作者： Shuai lu Yingzhu Chen Kun Qin jianfang Zhou Yongliang Lou Wenjie Tan Institute of Medical Virology Wenzhou Medical University National Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and Prevention

To characterize the antigenicity of nucleocapsid proteins(NP) derived from canine coronavirus(CCo V) and canine respiratory coronavirus(CRCo V) in China, the N genes of CCo V(CCo V-BJ70) and CRCo V(CRCo V-BJ202) were cloned from swabs obtained from diseased pet dogs in Beijing and then sequenced. The recombinant NPs(r NPs) were expressed in Escherichia coli and purified by nickel-affinity column and size exclusion chromatography. Sequencing data indicated that the N genes of CCo V-BJ70 and CRCo V-BJ202 belonging to two distinctly different groups were relatively conserved within each subgroup. Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) results showed that r NPs of CCo V and CRCo V were expressed efficiently and isolated with a final purity of over 95%. Western blot analysis revealed the r NP from CRCo V could cross-react with mice antisera against human coronavirus(HCo V-229 E, NL63, OC43, HKU1), while r NP of CCo V had cross-reactivity with only anti-sera against viruses belonging to the same group(HCo V-229 E and NL63). In summary, CCo V and CRCo V r NPs were successfully expressed in E. coli and showed antigenic cross-reactivity with antisera raised against human coronaviruses. These findings indicate that further serologic studies on coronavirus infections at the animal-human interface are needed.

关键词： canine coronavirus canine respiratory coronavirus nucleocapsid protein expression cross-reactivity

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肝移植高级护理实践模式的构建与实施效果

中国护理管理 2023年第5期23卷 650-653页

作者：冯志仙沈鸣雁鲁剑芳张园园陶丽燕路杨万鹏夏金亚仙黄则勇陈翔董迪树兰(杭州)医院护理部浙江树人学院树兰国际医学院杭州市310004 树兰(杭州)医院肝移植中心浙江树人学院树兰国际医学院杭州市310004 树兰(杭州)医院肝移植监护室浙江树人学院树兰国际医学院杭州市310004 树兰(杭州)医院手术室和麻醉复苏室浙江树人学院树兰国际医学院杭州市310004

目的:构建肝移植高级护理实践模式并总结实践经验与挑战。方法:分析杭州市某三级甲等医院肝移植护理实践需求和护理模式,设置肝移植高级实践护理岗位,明确工作模式、职责和人员分工,观察并评价实施效果。结果:选拔6名护士组成院内肝移... 详细信息

目的:构建肝移植高级护理实践模式并总结实践经验与挑战。方法:分析杭州市某三级甲等医院肝移植护理实践需求和护理模式,设置肝移植高级实践护理岗位,明确工作模式、职责和人员分工,观察并评价实施效果。结果:选拔6名护士组成院内肝移植高级实践护理小组,该小组于2020年—2022年共参加多学科诊疗讨论596例、参与疑难危重症肝移植患者照护249例、护理会诊162次;完成肝移植患者随访工作,失访率为0,随访档案完整率为100.00%;主持9项质量改善项目;肝移植护理质量指标较实施前提升。结论:本研究构建的肝移植高级护理实践模式,有助于深入开展高质量临床照护,提高护理水平,促进学科持续发展。

关键词：肝移植护理模式高级实践护理

Edge-face chromatic number of Halin-graphs

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Chinese Science Bulletin 1999年第2期44卷 189-190页

作者： Zhongfu Zhang Xinzhong lu Linzhong Liu jianfang Wang Tongxin Gu Lanzhou Railway Inst Inst Appl Math Lanzhou 730070 Peoples R China Chinese Acad Sci Inst Appl Math Beijing 100080 Peoples R China Chinese Acad Sci Res Ctr Comp Sci Hefei Branch Hefei 230031 Peoples R China

Definition 1. Assume that G（V, E, F）is a 3-connected plane graph. Remove all edges on the boundary of a face f0 whose degree of all vertices of $ V（f-0）$ is 3 such that G becomes a tree T whose degree of all verti... 详细信息

关键词： Edge-face chromatic number of Halin-graphs

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剖宫产术后硬膜外自控镇痛的疗效

中国临床药学杂志 2008年第5期17卷 293-294页

作者：郑桂琴卢健芳巫世娟李佳倪金爱红丘海燕南方医科大学附属深圳第二人民医院妇产科深圳518035 南方医科大学附属深圳第二人民医院麻醉科深圳518035

目的观察剖宫产术后硬膜外自控镇痛的效果及对子宫收缩的影响。方法60例患者剖宫产术后随机分为观察组30例(剖宫产手术结束后留置硬膜外导管施行术后自控镇痛)和对照组30例(手术结束后即拔除硬膜外导管不施行术后自控镇痛,必要时肌内注... 详细信息

目的观察剖宫产术后硬膜外自控镇痛的效果及对子宫收缩的影响。方法60例患者剖宫产术后随机分为观察组30例(剖宫产手术结束后留置硬膜外导管施行术后自控镇痛)和对照组30例(手术结束后即拔除硬膜外导管不施行术后自控镇痛,必要时肌内注射哌替啶)。观察术后镇痛效果、对子宫收缩、产后阴道出血量及对哺乳的影响。结果剖宫产术后施行术后自控镇痛其镇痛效果显著,产妇伤口疼痛明显减轻,促进机体的恢复;对子宫收缩无不良影响,不增加产后阴道出血量;并能做到早期哺乳,促进母乳喂养。结论硬膜外自控镇痛效果良好,对子宫收缩无影响,不增加术后阴道出血量。

关键词：硬膜外腔镇痛剖宫产术

Asymmetric Distribution of Convection in Tropical Cyclones over the Western North Pacific Ocean

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Advances in Atmospheric Sciences 2016年第11期33卷 1306-1321页

作者： lu YANG jianfang FEI Xiaogang HUANG Xiaoping CHENG Xiangrong YANG Juli DING Wenli SHI Institute of Meteorology and Oceanography PLA University of Science and Technology

Forecasts of the intensity and quantitative precipitation of tropical cyclones（TCs） are generally inaccurate, because the strength and structure of a TC show a complicated spatiotemporal pattern and are affected by various factors. Among these, asymmetric convection plays an important role. This study investigates the asymmetric distribution of convection in TCs over the western North Pacific during the period 2005–2012, based on data obtained from the Feng Yun 2（FY2）geostationary satellite. The asymmetric distributions of the incidence, intensity and morphology of convections are *** show that the PDFs of the convection occurrence curve to the azimuth are sinusoidal. The rear-left quadrant relative to TC motion shows the highest occurrence rate of convection, while the front-right quadrant has the lowest. In terms of intensity, weak convections are favored in the front-left of a TC at large distances, whereas strong convections are more likely to appear to the rear-right of a TC within a 300 km range. More than 70% of all MCSs examined here are elongated systems, and meso-β enlongated convective systems（MβECSs） are the most dominant type observed in the outer region of a TC. Smaller MCSs tend to be more concentrated near the center of a TC. While semi-circular MCSs [MβCCSs, MCCs（mesoscale convective complexes）] show a high incidence rate to the rear of a TC, elongated MCSs [MβECSs, PECSs（persistent elongated convective systems）] are more likely to appear in the rear-right quadrant of a TC within a range of 400 km.

关键词： convection Tropical convective asymmetric tropical mesoscale elongated typhoon likely storm

Identifying Intraspecific Variation in Venom Yield of Chinese Cobra(Naja atra) from Ten Populations in China's Mainland

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Asian Herpetological Research 2019年第1期10卷 32-40页

作者： jianfang GAO Yin YIN Yanfu QU Jin WANG Longhui LIN Hongliang lu Xiang JI Hangzhou Key Laboratory for Animal Adaptation and Evolution College of Life and Environmental SciencesHangzhou Normal University Jiangsu Key Laboratory for Biodiversity and Biotechnology College of Life Sciences Nanjing Normal University

Detailed information on venom yield is helpful in preparing antivenoms and treating snakebites, but such information is lacking for many species of venomous snakes. The Chinese cobra(Naja atra) is a large sized, venomous snake commonly found in southeastern China, where it causes a heavy burden of snakebites. To examine the effects of various factors(morphology, sex, age, season, and geographical origin) on the venom yield in this snake, we collected venom samples of 446 individuals(426 adults and 20 neonates) from 10 populations of N. atra over an eightyear period. We used two variables, lyophilized venom mass(venom yield) and solid content of venom(% solids), to quantify the venom yield. We used linear regression analysis to check if venom yield was related to morphological factors, one-way ANOVA and one-way ANCOVA to detect the sexual, ontogenetic, and geographic variation in venom yield, and repeated-measures ANOVA to examine seasonal shifts in venom yield. Our results indicate that venom yield of N. atra is positively related to the morphological traits examined, with male snakes expelling more venom than females. Venom yield in N. atra was age-related, with elder snakes always expelling more venom than younger ones. Geographic variation in venom yield was also observed, while seasonal variation was not. The solid content of venom was lower in males than in females, but this was not related to morphology, season, age, or geography. Our findings suggest that venom yield in N. atra is influenced by multiple factors, as well as by the interactions among these factors.

关键词： Naja atra venom yield intraspecific variation snakebite

场景自适应的红外焦平面成像动态范围调整技术

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强激光与粒子束 2014年第3期26卷 15-19页

作者：贺明王亚弟王新赛张成斌张天一路建方孙雨宸信息工程大学电子技术学院郑州450052 防空兵学院郑州450052 上海蓝剑科技发展有限公司上海201821

针对红外焦平面对辐射强度较大的目标输出动态范围不足的问题,提出了一种场景自适应的红外焦平面成像动态范围调整技术。包括3个方面的内容:从图像中提取目标灰度特征,获取动态范围自适应依据;结合最小均方自适应滤波算法,对调整依据进... 详细信息

针对红外焦平面对辐射强度较大的目标输出动态范围不足的问题,提出了一种场景自适应的红外焦平面成像动态范围调整技术。包括3个方面的内容:从图像中提取目标灰度特征,获取动态范围自适应依据;结合最小均方自适应滤波算法,对调整依据进行滤波预测后给出调节值;把灰度调节值转换成电平值,利用电平值设置红外焦平面偏置电压完成焦平面成像动态范围自适应。最后,对整体方案进行了实验验证。通过在红外焦平面成像系统中实验证明了基于场景成像动态范围自适应方法的可行性,并获得了很好的效果,成像质量有明显提高。

关键词：场景自适应动态范围红外焦平面自适应滤波器