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The Crop Journal 2021年第2期9卷 456-464页

作者： Shuo Zhou Cuimian Zhang He Zhao Mengyu Lyu Fushuang Dong Yongwei Liu Fan Yang Haibo Wang jianfang chai Hebei Province Plant Genetic Engineering Center Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry SciencesShijiazhuang 050051HebeiChina

Wheat-rye T1BL·1RS translocation lines are widely used,especially in China,but their processing quality is generally *** interfering expression vector targeting theω-secalin genes was constructed with the 1Bx7 seed-specific ***-mediated genetic transformation of the wheat cultivar KN199 carrying the T1BL·1RS translocation generated 10 transgenic *** representative transgenic lines,8-2 and 13-7,were selected for *** with the control,the two transformants showed an up to 4.5-fold decrease in totalω-secalins and various levels of decrease inω-gliadins,γ-gliadins,and low-molecular-weight glutenins.A decrease in high molecular weight(HMW)glutenin 1Bx7 was detected only in 8-2,owing possibly to promoter *** levels ofα-gliadins were observed in both transformants,but increased levels of HMW glutenins were observed only in *** 13-7 showed increases in gluten index,Zeleny sedimentation value,stabilization time,and maximum *** bread volume was 849.6 mL,an 11.9%increase over that of the *** 8-2 showed decreases in these parameters,but its total cake-making quality score was 88,an 17.3%increase over that of the *** study demonstrates that the same RNAi construct may produce different effects on wheat processing quality and highlights the influence of the vector promoter in RNA interference.

关键词： T1BL·1RS Quality improvement RNA interference Secalin Triticum aestivum

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Synthesis, Characterization and Performance Evaluation of an Advanced Solid Electrolyte and Air Cathode for Rechargeable Lithium-Air Batteries

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Journal of Materials Science and Chemical Engineering 2016年第1期4卷 74-89页

作者： Susanta K. Das jianfang chai Salma Rahman Abhijit Sarkar Department of Mechanical Engineering Kettering University Flint Michigan USA Michigan Molecular Institute Midland Michigan USA

Synthesis and characterization of a tri-layered solid electrolyte and oxygen permeable solid air cathode for lithium-air battery cells were carried out in this investigation. Detailed fabrication procedures for solid electrolyte, air cathode and real-world lithium-air battery cell are described. Materials characterizations were performed through FTIR and TGA measurement. Based on the experimental four-probe conductivity measurement, it was found that the tri-layered solid electrolyte has a very high conductivity at room temperature, 23。C, and it can be reached up to 6 times higher at 100。C. Fabrication of real-world lithium-air button cells was performed using the synthesized tri-layered solid electrolyte, an oxygen permeable air cathode, and a metallic lithium anode. The lithium-air button cells were tested under dry air with 0.1 mA - 0.2 mA discharge/ charge current at elevated temperatures. Experimental results showed that the lithium-air cell performance is very sensitive to the oxygen concentration in the air cathode. The experimental results also revealed that the cell resistance was very large at room temperature but decreased rapidly with increasing temperatures. It was found that the cell resistance was the prime cause to show any significant discharge capacity at room temperature. Experimental results suggested that the lack of robust interfacial contact among solid electrolyte, air cathode and lithium metal anode were the primary factors for the cell’s high internal resistances. It was also found that once the cell internal resistance issues were resolved, the discharge curve of the battery cell was much smoother and the cell was able to discharge at above 2.0 V for up to 40 hours. It indicated that in order to have better performing lithium-air battery cell, interfacial contact resistances issue must have to be resolved very efficiently.

关键词： Lithium-Air Solid Electrolyte Air Cathode Button Cell Metallic Lithium

小麦1B/1R易位系中有活性的ω黑麦碱基因的启动子的克隆与功能验证

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农业生物技术学报 2011年第3期19卷 477-482页

作者：柴建芳河北省农林科学院遗传生理研究所/河北省植物转基因中心

小麦1B/1R易位系由于具有高产和适应性广等优点,在我国的小麦生产中得到了广泛的应用,但这类品种有一个共同的缺点,就是加工品质比较差,ω-黑麦碱被认为是影响其加工品质的一个重要因素。通过RNA干扰途径沉默ω-黑麦碱基因的表达,是改... 详细信息

小麦1B/1R易位系由于具有高产和适应性广等优点,在我国的小麦生产中得到了广泛的应用,但这类品种有一个共同的缺点,就是加工品质比较差,ω-黑麦碱被认为是影响其加工品质的一个重要因素。通过RNA干扰途径沉默ω-黑麦碱基因的表达,是改良小麦1B/1R易位系加工品质的一个重要策略。使用由ω-黑麦碱基因自身启动子驱动的RNA干扰表达载体,可使转入的基因在需要的部位和需要的时间表达,提高分子育种的效果。为获得有活性的ω黑麦碱基因的启动子,本研究设计了覆盖启动子区和部分编码区的一对特异引物,以小麦(Triticumaestivum)1B/1R易位系兰考906为试材,通过PCR克隆得到了与3个有转录活性的ω-黑麦碱基因有对应关系的5个启动子A9-1、H2-1、H7-1、C11和F11-1,选择与其中2个有转录活性的ω-黑麦碱基因分别有对应关系的启动子A9-1和F11-1构建以GUS为标记基因的表达载体,并用基因枪对小麦幼嫩种子进行了轰击,通过对GUS基因的瞬时表达分析,证实其中一个启动子F11-1具有活性。研究结果为构建由ω黑麦碱基因自身启动子驱动的RNA干扰表达载体提供了基础资料。

关键词：小麦 1B/1R易位系 ω-黑麦碱启动子克隆功能验证

二茂铁吩开环聚合合成茂金属有机高分子研究进展

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有机化学 2001年第2期21卷 126-133页

作者：贾文利唐良富柴建方王积涛南开大学化学系

综述了近年来二茂铁吩化合物的合成及其开环反应的研究进展,同时总结了聚茂金属有机高分子的性质及其应用.

关键词：二茂铁吩开环聚合反应茂金属有机高分子合成

Effect of Low Temperature Treatment on Agrobacterium-mediated Transformation of Maize Seed Buds

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Agricultural Biotechnology 2018年第3期7卷 75-76页

作者： Mengyu LYU Fushuang DONG Junmin ZHANG He ZHAO jianfang chai Shuo ZHOU Fan YANG Yongwei LIU Haibo WANG Plant Genetic Engineering Center of Hebei Province Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry Sciences

[Objectives]This study was conducted to improve the genetic transformation rate of maize. [Methods]The seeds of maize ＂ Zheng 58＂ as the experimental material were germinated and treated under freezing temperature of-18 ℃ for 30 and 35 min. The EGFP gene was transformed into the growth points of these seed buds by Agrobacterium tumefaciens EHA105. The transformation effect was determined by fluorescence protein detection on transformed buds. [Results]After a certain period of treatment at-18 ℃ following germination and transformation of maize bud growth points with A. tumefaciens,although the survival rate of maize buds was reduced,the percentage of transformed cells significantly increased. [Conclusions]Low temperature treatment can improve the transformation efficiency of A. tumefaciens to the growth point of maize bud.

关键词： Maize buds Low temperature treatment Agrobacterium Transgenic

Study on Differentiation of Growing Points in Corn Sprouts into Multiple Stems

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Agricultural Biotechnology 2020年第1期9卷 43-44页

作者： Mengyu LYU Fushuang DONG jianfang chai Shuo ZHOU He ZHAO Yongwei LIU Fan YANG Xiaoyu LI Junmin ZHANG Haibo WANG Institute of Genetics and Physiology Hebei Academy of Agriculture and Forestry Sciences/Plant Genetic Engineering Center of Hebei ProvinceShijiazhuang 050051China

[Objectives] This study was conducted to improve the efficiency of genetic transformation using growing points of corn sprouts. [Methods] A mixed liquid of plant hormones 6-BA and KT at a suitable concentration and ratio was added dropwise to the growing points of corn sprouts, followed by culture under appropriate conditions. [Results] Corn sprouts could be induced to form multiple plants, thereby improving the transformation efficiency of growing points in corn sprouts. [Conclusions] This study provides a breeding method using growing points of corn sprouts as transformation receptors for direct plantlet formation without tissue culture by the Agrobacterium transgenic technology.

关键词： Corn Sprout growing point Differentiation Plant

RNA沉默及其在植物基因功能研究和作物改良中的应用

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河北农业科学 2003年第B9期7卷 33-39页

作者：柴建芳河北省农林科学院遗传生理研究所石家庄050051

RNA沉默是指导入或细胞内转录合成的双链RNA会特异性地降解具有同源序列的mRNA,从而导致内源的、外源的和病毒基因的沉默,它是真核生物特有的一种阻止转座子转座和抵御外源DNA入侵的防御机制。利用RNA沉默技术,人们正对大量基因进行功... 详细信息

RNA沉默是指导入或细胞内转录合成的双链RNA会特异性地降解具有同源序列的mRNA,从而导致内源的、外源的和病毒基因的沉默,它是真核生物特有的一种阻止转座子转座和抵御外源DNA入侵的防御机制。利用RNA沉默技术,人们正对大量基因进行功能研究,并在作物性状改良方面取得了一些可喜成果。

关键词： RNA沉默植物基因功能作物改良应用同源序列病毒基因真棱生物作物性状

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创造性思维系统特征初探——兼谈与美育的密切关系

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山西高等学校社会科学学报 2007年第1期19卷 13-19页

作者：柴建芳山西大学工程学院山西太原030013

人的思维创造活动是作为开放系统而存在的,人是思维的主体,必须远离常规思维的原来平衡态。只有在这种自由创造状态中,才有可能出现新的思维涨落,这种涨落会由于系统内部非线性作用的交互相干效应而迅速放大,形成巨涨落,演化成为新的思... 详细信息

人的思维创造活动是作为开放系统而存在的,人是思维的主体,必须远离常规思维的原来平衡态。只有在这种自由创造状态中,才有可能出现新的思维涨落,这种涨落会由于系统内部非线性作用的交互相干效应而迅速放大,形成巨涨落,演化成为新的思维创造活动的有序结构。本文从系统理论角度分析创造性思维系统的运作及形成条件。在这种系统中,非逻辑思维占有重要位置,直觉、灵感、想象则成为创造过程的精华。而且,创造性思维的培养是与美育密切相关的。

关键词：创造性思维系统特征非线性美育

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Effects of Acetylacetone Solution Soaking on Agrobacterium - transformed Maize Seed Buds

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Agricultural Biotechnology 2021年第3期10卷 11-14页

作者： Mengyu LYU Fushuang DONG Shuo ZHOU jianfang chai He ZHAO Yongwei LIU Fan YANG Bo JIAO Xiaoyu LI Junmin ZHANG Haibo WANG Plant Genetic Engineering Center of Hebei Province Institute of Genetics and PhysiologyHebei Academy of Agriculture and Forestry Sciences(Agricultural Product Quality and Safety Research Center of Hebei Academy of Agricultural and Forestry Sciences)Shijiazhuang 050051China

[Objectives]The effect of acetosyringone seed soaking on the transformation of maize seed buds was analyzed,so as to improve the genetic transformation efficiency of maize and to provide technical support for transgenic breeding of maize.[Methods]The seeds of the"Zheng 58"maize inbred line were used as experimental *** the seeds were germinated,AS was added to the water at concentrations of 70,140,210,and 280μmol/L,respectively,and the seeds germinated without the addition of AS served as the *** Agrobacterium-mediated method was used to transform bud growth points of maize seeds,and green fluorescent protein detection was performed on the young shoots transformed with EGFP(enhanced green fluorescent protein)*** effect of soaking seeds with acetosyringone solution on the transformation of maize bud growth points by Agrobacterium was studied according to the detection results.[Results]Soaking seeds in acetosyringone solutions for germination had the effect of inhibiting the germination of maize seeds and inhibiting sprout elongation,and the higher the concentration of acetosyringone,the stronger the *** the concentration of acetosyringone solution was 280μmol/L,the germination rate of seeds was only 36.2%of the CK,while soaking seeds with 70-140μmol/L acetosyringone solution for germination could not only ensure a higher germination rate of maize seeds,but also significantly increased the transformation efficiency of maize bud growth *** the seeds were soaked with 70μmol/L acetosyringone solution for germination,the positive rate of transformed maize buds was the highest,reaching 32.1%.[Conclusions]When maize bud growth points were used as the receptor of Agrobacterium transformation,soaking seeds with 70-140μmol/L acetosyringone for germination basically did not affect the germination of seeds,and was beneficial to the activation of Agrobacterium,thereby promoting the transformation.

关键词： Maize Acetosyringone Seed soaking Transgenic efficiency

An antiquitin promoter from wheat and its application in enhancing plant nitrogen utilization efficiency

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An antiquitin promoter from wheat and its application in enh...