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Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain

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The Crop Journal 2024年第1期12卷 262-270页

作者： Yi Jiang Wenli Tao Weiyang Zhang Zhiqin Wang jianchang Yang Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou UniversityYangzhou 225009JiangsuChina

Lysine content is a criterion of the nutritional quality of *** the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional ***,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature *** content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling *** increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to *** of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or *** and elevated BR levels during grain filling increased lysine biosynthesis in *** in lysine biosynthesis in rice should focus on IG.

关键词： Brassinosteroids Inferior grain Lysine biosynthesis Rice Wetting alternating with partial drying

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计算机试卷处理系统

南京邮电学院学报 1989年第4期 84-91页

作者：杜建昌南京邮电学院基础课部

本系统将全部考试后处理工作溶于一体,对考试作出全面质量分析,评估教学考试质量,并以10种方式评分.系统结构采用程序设计语言与数据库管理系统相结合的方式,计算速度快,占用内存少,实用性强.

关键词：试卷考试计算机辅助教学

TARE1,a mutated copia-like LTR retrotransposon followed by recent massive amplification in tomato

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TARE1,a mutated copia-like LTR retrotransposon followed by r...

Multiparameter collaborative optimization of the vibrating screen based on the behavior of oil sunflower seed penetrating screen holes

第十四届全国植物基因组学大会

作者： Hao Yin Jing Liu Yingxiu Xu Xing Liu jianchang du Bioinformatics Group Institute of Industrial CropsJiangsu Academy of Agricultural Sciences

Long terminal repeat retrotransposons(LTR-RTs) are the major DNA components in flowering *** LTR-RTs contain dinucieotides’TG’and’CA’at the ends of the two *** we report the structure,evolution,and propensity of a... 详细信息

关键词： TARE1 retrotransposon mutation divergence domestication genome evolution tomato

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International Journal of Agricultural and Biological Engineering 2024年第1期17卷 49-58页

作者： Wenbo Wei jianchang Li Jianjun Hao Maohua Xiao Huiju Zhang College of Mechanical and Electrical Engineering Hebei Agricultural UniversityBaoding 071001HebeiChina College of Engineering Nanjing Agricultural UniversityNanjing 210032China The Higher Educational Key Laboratory for Flexible Manufacturing Equipment Integration of Fujian Province Xiamen Institute of TechnologyXiamen 361000FujianChina

The behavior of oil sunflower seeds penetrating screen holes is an important factor that affects the screening performance of oil sunflower *** this study,a double-deck reverse-motion vibrating screening device for oil sunflower seed screening was *** force condition and motion law of the oil sunflower seeds on the screen surface were *** study compared the effect of particle filling amount of discrete element model of oil sunflower seeds on the simulation *** screening process was numerically simulated using the coupled Discrete Element Method and Multibody Dynamics(DEM-MBD)technique with the screening percentage of oil sunflower seeds as the *** influence of the operating parameters of the vibrating screen on the screening effect was analyzed using a multiparameter collaborative optimization *** results of this study can provide a reference for the numerical simulation of crop screening behavior and the development of screening devices.

关键词： oil sunflower seeds screening analysis DEM-MBD discrete element method response surface analysis

马铃薯(Solanum tuberosum L.)全基因组水平上LTR-逆转座子的鉴定与进化分析

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基因组学与应用生物学 2013年第6期32卷 734-742页

作者：许莹修杜建厂江苏省农业科学院经济作物研究所生物信息项目组农业部长江下游棉花与油菜重点实验室南京210014

LTR-逆转座子是构成基因组特别是植物基因组的重要组分。它们在寄主基因组的进化过程中起到重要作用。马铃薯是重要的经济作物和粮食作物,其全基因组序列的公布为进一步研究其遗传组成和演化提供了基础。本文以马铃薯全基因组序列为材料... 详细信息

LTR-逆转座子是构成基因组特别是植物基因组的重要组分。它们在寄主基因组的进化过程中起到重要作用。马铃薯是重要的经济作物和粮食作物,其全基因组序列的公布为进一步研究其遗传组成和演化提供了基础。本文以马铃薯全基因组序列为材料,用结构分析和同源比对的方法分离得到9 318个完整的LTR-逆转座子,7 281个非完整(truncated)LTR-逆转座子元件和3 657个solo LTR元件。进一步研究表明,gypsy类转座子在距今两百万年(million years ago,MYA)时转座活性被抑制,而copia类元件活跃至今。马铃薯和番茄比较基因组学的研究表明,LTR-逆转座子序列变异率为18.73%,远高于基因序列的7.37%和CDS序列的5.01%。

关键词： LTR-逆转座子基因组序列插入时间马铃薯

大豆HGm1 helitron转座元件的鉴定与生物信息学分析

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南京农业大学学报 2016年第4期39卷 527-533页

作者：彭珍刘静郭月李英杜建厂南京农业大学园艺学院/作物遗传与种质创新国家重点实验室江苏南京210095 江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室江苏南京210014

[目的]helitron转座元件是近年来发现的一种结构新颖和转座方式独特的DNA转座子。我们从全基因组水平上对大豆基因组中拷贝数最多的helitron HGm1家族进行深入分析,旨在为后续进一步明确helitron转座元件在大豆及其近缘种基因和基因组... 详细信息

[目的]helitron转座元件是近年来发现的一种结构新颖和转座方式独特的DNA转座子。我们从全基因组水平上对大豆基因组中拷贝数最多的helitron HGm1家族进行深入分析,旨在为后续进一步明确helitron转座元件在大豆及其近缘种基因和基因组演化中所起的作用提供帮助。[方法]利用基因组学和生物信息学的研究手段,我们对大豆HGm1家族的数量、结构特征、染色体分布、插入基因情况,基因片段的捕获及表达模式等进行了详细的研究。[结果]从公开发表的大豆基因组序列中共鉴定出254个HGm1转座元件。这些元件的3'末端十分保守,主要由GC含量较高的碱基组成。HGm1元件主要分布在AT丰富的区域,并在重组受到显著抑制的异染色质区域富集。研究还发现,68个元件被83个外源的转座元件插入;64个元件插入到编码蛋白的基因附近(<2 kb);115个元件携带154个完整的外源基因;52个元件有潜在的转录活性,在根、茎、叶、花和种子中,以及干旱胁迫和组织培养条件都有所表达。[结论]与玉米中有关helitron的研究结果相似,HGm1家族在大豆基因组中经常以巢式的结构存在,具有频繁插入到基因附近和捕获基因的现象,并且部分成员具有潜在的转录活性。而与玉米helitron不同的是,大豆HGm1 helitron家族主要分布在基因贫乏的异染色质区域。因此,HGm1家族在改变大豆基因结构和调节基因表达以及影响大豆基因组的演化过程中具有较为重要的作用。

关键词：大豆 helitron转座元件分布插入捕获转录

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18阶S波段高温超导滤波器的研制

低温物理学报 2015年第2期37卷 149-152页

作者：杜健昌刘永霞季来运天津理工大学电子信息工程学院天津海泰超导电子有限公司

本文设计制作了一款18阶高温超导滤波器,滤波器工作频率在S波段,中心频率为3128MHz,相对带宽约为19%,滤波器结构采用谐振器终端加载插指电容的方法,有效解决了滤波器二倍频的寄生通带问题,二倍频谐波得到了很好的抑制.本工作进行了滤波... 详细信息

本文设计制作了一款18阶高温超导滤波器,滤波器工作频率在S波段,中心频率为3128MHz,相对带宽约为19%,滤波器结构采用谐振器终端加载插指电容的方法,有效解决了滤波器二倍频的寄生通带问题,二倍频谐波得到了很好的抑制.本工作进行了滤波器设计理论分析,并采用Sonnet软件对微带电路进行全波电磁场仿真,滤波器最终由制备于0.5mm厚的2英寸氧化镁(MgO)衬底上的镝钡铜氧(DyBCO)双面超导薄膜制作而成,取得较为满意的结果.

关键词：高温超导滤波器二倍频抑制插指电容

Quantifying source-sink relationships in leaf-color modified rice genotypes during grain filling

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Journal of Integrative Agriculture 2024年第9期23卷 2923-2940页

作者： Zhenxiang Zhou Paul CStruik Junfei Gu Peter E.L.van der Putten Zhiqin Wang jianchang Yang Xinyou Yin Centre for Crop Systems Analysis Department of Plant SciencesWageningen University&ResearchWageningen 6700 AKThe Netherlands College of Agriculture Yangzhou UniversityYangzhou 225009China

Leaf-color modification can affect canopy photosynthesis,with potential effects on rice yield and yield *** source-sink relationships through crop management is often used to improve crop *** study investigated whether and how modifying leaf color alters source-sink relationships and whether current crop cultivation practices remain applicable for leaf-color modified *** collected data of total biomass and nitrogen(N)accumulation in rice genotypes of four genetic backgrounds and their leaf-color modified variants(greener or yellower)were analyzed,using a recently established modelling method to quantify the source-sink(im)balance during grain *** all leaf-color variants,only one yellower-leaf variant showed a higher source capacity than its normal *** was associated with greater post-flowering N-uptake that prolonged the functional leaf-N duration,and this greater post-flowering N-uptake was possible because of reduced pre-flowering N-uptake.A density experiment showed that current management practices(insufficient planting density accompanied by abundant N application)are unsuitable for the yellower-leaf genotype,ultimately limiting its yield ***-color modification affects source-sink relationships by regulating the N trade-off between pre-and post-flowering uptake,as well as N translocation between source and sink *** best exploit leaf-color modification for improving crop productivity,adjustments of crop management practices are required.

关键词： source-sink relationship biomass nitrogen Oryza sativa leaf-color modification

Hyperglycemia activates FGFR1 via TLR4/c-Src pathway to induce inflammatory cardiomyopathy in diabetes

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Acta Pharmaceutica Sinica B 2024年第4期14卷 1693-1710页

作者： Xiong Chen Jinfu Qian Shiqi Liang jianchang Qian Wu Luo Yujuan Shi Hong Zhu Xiang Hu Gaojun Wu Xiaokun Li Guang Liang Department of Endocrinology the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China Chemical Biology Research Center School of Pharmaceutical SciencesWenzhou Medical UniversityWenzhou 325035China Department of Wound Repair the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China Department of Cardiology the First Affiliated HospitalWenzhou Medical UniversityWenzhou 325035China School of Pharmaceutical Sciences Hangzhou Medical CollegeHangzhou 311399China

Protein tyrosine kinases (RTKs) modulate a wide range of pathophysiological events in several non-malignant disorders, including diabetic complications. To find new targets driving the development of diabetic cardiomyopathy (DCM), we profiled an RTKs phosphorylation array in diabetic mouse hearts and identified increased phosphorylated fibroblast growth factor receptor 1 (p-FGFR1) levels in cardiomyocytes, indicating that FGFR1 may contribute to the pathogenesis of DCM. Using primary cardiomyocytes and H9C2 cell lines, we discovered that high-concentration glucose (HG) transactivates FGFR1 kinase domain through toll-like receptor 4 (TLR4) and c-Src, independent of FGF ligands. Knocking down the levels of either TLR4 or c-Src prevents HG-activated FGFR1 in cardiomyocytes. RNA-sequencing analysis indicates that the elevated FGFR1 activity induces pro-inflammatory responses via MAPKs–NFκB signaling pathway in HG-challenged cardiomyocytes, which further results in fibrosis and hypertrophy. We then generated cardiomyocyte-specific FGFR1 knockout mice and showed that a lack of FGFR1 in cardiomyocytes prevents diabetes-induced cardiac inflammation and preserves cardiac function in mice. Pharmacological inhibition of FGFR1 by a selective inhibitor, AZD4547, also prevents cardiac inflammation, fibrosis, and dysfunction in both type 1 and type 2 diabetic mice. These studies have identified FGFR1 as a new player in driving DCM and support further testing of FGFR1 inhibitors for possible cardioprotective benefits.

关键词： Diabetic cardiomyopathy Protein tyrosine kinases FGFR1 Cardiomyocytes Inflammatory responses Toll-like receptor4 c-Src NFkB