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Research on the Construction and Function of a Chlorophyll Degradation Recombinant Engineering Strain

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Agricultural Science & Technology 2017年第10期18卷 1788-1794页

作者： Zongcan YANG Zhan ZHANG jianbin ye Yufu PENG Xiangzhen LIU Hongxia WANG Technology Center China Tobacco Henan Industrial Co. Ltd. School of Food and Biological Engineering Zhengzhou University of Light Industry

In order to effectively reduce the chlorophyll content in flue-cured tobacco, improve the overall quality of tobacco leaves, chlorophyllase gene was cloned from Arabidopsis thaliana. After the expression of the expression vector in E. coil, the recombinant engineering strain was obtained. Afterwards, IPTG （isopropy-β-D-thiogalactopyranoside）was used to induce the goal protein, and the chlorophyllase activity of the recombinant engineering strain was measured, so as to investigate its degradation effect on the chlorophyll in the extracts of tobacco leaves. The results were as follows：（1） the amplified chlorophyllase gene At- CLH1 constructed the expression vector pET28a-AtCLH1 successfully, obtaining the recombinant engineering strain; （2） induced under 30 ℃ for 22 h, the strain could well express the recombinant protein AtCLH1 with 0.5 mmol/L IPTG, and the molecular weight was about 35 kDa; （3） the strain showed good chlorophyllase producing capability, and the activity of the produced chlorophyllase could reach up to 24.9 U/mL, which could degrade the chlorophyll in tobacco extract and had a good application prospect in improving the quality of low quality tobacco; （4） based on the results of orthogonal test, the enzyme extract from the strain was added to the tobacco leaf surface, which could make the degradation rate of chlorophyll in the tobacco leaf reach 17.06% under the temperature of 37 ℃ at the humidity of 75% for 48 h; （5） after treated by the enzyme liquid, the test tobacco showed increase in the content of aromatic substances, enhancement of tobacco fragrance quality and amount, significant decrease of offensive odor and irritation, significant improvement of agreeable aftertaste, making the overall sensory quality of the tobacco leaf significantly improved.

关键词： Tobacco leaf Degradation of chlorophyll Recombinant Engineering strain Protein expression Activity of chlorophyllase Orthogonal test Aroma substances Sensory quality

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风电场群远程集中SCADA系统设计

电力系统自动化 2010年第23期34卷 97-101页

作者：叶剑斌左剑飞黄小鉥国网电力科学研究院/南京南瑞集团公司江苏省南京市210061 西安供电局长安分局陕西省西安市710100

随着国家清洁能源战略的实施,风电场的数量和规模都快速、大幅度增加。同时,由于各种新型技术的进步,风电场群远程集中数据采集与监控(SCADA)系统的建立具备了必要的技术条件。以传统电力调配一体化系统为基础,参照公共信息模型(CIM)和I... 详细信息

随着国家清洁能源战略的实施,风电场的数量和规模都快速、大幅度增加。同时,由于各种新型技术的进步,风电场群远程集中数据采集与监控(SCADA)系统的建立具备了必要的技术条件。以传统电力调配一体化系统为基础,参照公共信息模型(CIM)和IEC61400-25标准,通过对其模型、数据和功能方面的改进和扩展,实现了风电场群远程集中SCADA系统,并在长江新能源开发有限公司上海控制中心得到成功应用。

关键词：风电场群数据采集与监控 IEC61400-25 公共信息模型滑动平均清洁能源

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基于图像识别的电能表智能建档系统研究

电测与仪表 2020年第24期57卷 139-144页

作者：赵兴旺叶剑斌南瑞集团有限公司国网电力科学研究院南京210003 国电南瑞南京控制系统有限公司南京210003

目前,省级计量检定中心正在向智能化、无人化、高可靠方向发展,但在电能计量设备到货建档环节仍采用人工维护方式。鉴于此,依据电能表主要建档参数均印刻在电能表表面、位置相对固定的特点,开展了基于图像识别的电能表智能建档技术研究... 详细信息

目前,省级计量检定中心正在向智能化、无人化、高可靠方向发展,但在电能计量设备到货建档环节仍采用人工维护方式。鉴于此,依据电能表主要建档参数均印刻在电能表表面、位置相对固定的特点,开展了基于图像识别的电能表智能建档技术研究,提出了基于SIFT特征区匹配方法、基于大津法的改进垂直投影直方图方法以及基于Bi-gram语言模型的电能表参数串识别后处理方法。应用上述技术研制了电能表智能建档系统,实现了单相电能表参数、三相电能表参数的自动识别和建档等功能。通过实验,验证了电能表智能建档系统可准确定位、切分和识别建档参数,为计量设备智能建档的推广应用提供理论和实践指导。

关键词：电能计量生产电能表智能建档图像识别识别后处理 Bi-gram

DLL3 in stomach cancer enhances cancer cell proliferation and regulates the immunity of tumor associated macrophages

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DLL3 in stomach cancer enhances cancer cell proliferation an...

第十三届全国免疫学学术大会

作者： jianbin ye Danlin Wu Junjie Wen Bingxin Hu Meiqun Luo Yan Li Yunshan Ning Southern Medical University

Background: Stomach cancer is the fifth common cancer and the third leading cause of cancer death worldwide and the second incidence and mortality of cancer in China. Tumor-associated macrophages(TAMs), as part of the adaptive immune response, constitute a major portion of the leukocyte infiltrate in cancer. Studies have demonstrated the connection between stomach cancer and macrophages. Notch signal pathway is important in the development of both tumor associated macrophages(TAMs) and stomach cancer, but how Notch in stomach cancer affects TAMs is barely understood. Methods: THP-1 was induced into macrophages by PMA and co-cultured with stomach cancer cell line MKN45. Notch signaling proteins were detected by western blot,and IL-1β, IL-12 and IL-10 were detected by ELISA in the supernatant from co-culture. The transcriptome sequencing was performed to detect the genes regulated by DLL3 overexpression in ***: We found that co-culture of macrophages and stomach cancer cell MKN45 could enhance MKN45 cell proliferation accompanied with activation of Notch1/Notch2 signaling and up-regulation of Notch ligand DLL3. Elevated DLL3 in MKN45 could promote cell proliferation, enhance activation of Notch1/Notch2 signaling, induce IL-33 expression, and lead to galectin-3-binding protein and heat shock cognate 71 kDa protein degradation in MKN45, which resulted in elevated IL-1β, IL-12 and IL-10 secretion by macrophages. Conclusions: This is the first report unveiling how Notch signal pathway especially DLL3 in stomach cancer regulates macrophages. And this will offer new possible targets for stomach cancer therapy.

关键词： Notch stomach cancer macrophages DLL3 IL-33

Th1-type cellular immune response in neonates induced by Group B streptococcus

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Th1-type cellular immune response in neonates induced by Gro...

第十三届全国免疫学学术大会

作者： Yanqing Lin Bingxin Hu Meiqun Luo jianbin ye Danlin Wu Junjie Wen Yan Li Yunshan Ning Southern Medical University

Background: Group B streptococcus(GBS) is one of the main pathogens of perinatal *** neonates infected with GBS in utero can suffer from sepsis, pneumonia, and *** innate immunity and adaptive immunity mediated by CD4T cells are activated in GBS-infected neonates, but the immune characteristics and the differentiation mechanism of CD4T cell are rarely *** the mechanism of GBS-induced differentiation of na?ve CD4T cells is crucial to elucidate the immune defense mechanism of neonates after ***: The Bio-Plex assay for detecting the cytokines and chemokines of 182 cases of neonatal umbilical cord blood, including 80 cases of GBS negative control group and 102 cases of GBS colonized group(43 symptomatic cases and 59 asymptomatic cases).Meanwhile, the na?ve CD4 T cells from neonatal umbilical cord blood were co-cultured with autologous monocytes stimulated by GBS, and master transcription factors were analyzed by ***: Compared to the control group detected by Bio-Plex, IFN-γ and CXCL10 in GBS-colonized group increased significantly, and IL-6 and IL-1β increased significantly only in symptomatic *** co-culture of na?ve CD4T cells, GBS and monocytes, Th1-related transcription factor T-bet was up-regulated, while Th2, Th17, Treg-related transcription factor GATA, RORγt and FoxP3 were ***: The INF-γ-producing Th1 cells is primary cellular immune response in neonates induced GBS.

关键词： Group B Streptococcus Th1-type cell differentiation INF-γ

Immunodominant CD4+T cell responses to Helicobacter pylori Lpp20

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Immunodominant CD4+T cell responses to Helicobacter pylori L...

第十二届全国免疫学学术大会

作者： Li Yan ye jianbin Wu Danlin Wen Junjie Zeng Shuang Wen Yuemei Jiang Yin Xi Yue Ning Yunshan Southern Medical University

Background:Antigen-specific CD4 T cells play an essential role in the immunopathogenesis of Helicobacter pylori(***) ***20,a conserved lipoprotein of H pylori,has been considered as one of major protective *** previous studies identified two Lpp20 H-2 d restricted CD4 T cell epitopes,which induced protective immunological response in mice against H pylori *** is an important feature of antibacterial cellular immune ***,few are known to immunodominant Lpp20-specific CD4 T cell response and Lpp20 CD4 T cell epitopes has not yet *** present study,we try to investigate the immunodominant CD4 T cells response to Lpp20 in ***-infected ***:Lpp20-specific CD4 T cells were expanded with autologous patient peripheral blood mononuclear cells that had been incubated with recombinant Lpp20 and characterized using 27 overlapping 18 mer synthetic peptides spanning the sequence of ***,the most potent core sequence of 18 mer peptides recognized by Lpp20-specific CD4 T cells was further identified using a set of 13 mer N-and C-terminus extended or truncated overlapping peptides covering the initially detected 18 mer ***:The immunodominant CD4 T cells responses specific to L and L were observed in most H pylori-infected *** patient H1,the immunodominant CD4 T cells responding to the L18-mer peptides recognized two 13-mer peptides(L and L).For patient H6,the immunodominant CD4 T cells responding to the L18-mer peptides recognized two 13-mer peptides(L and L).Conclusions:The characteristics of HLA class Ⅱ restriction to these immunodominant Lpp20 CD4 T cell epitptops and the correlation with various gastritis are under study,which will provide insight into mechanisms of protective immunity and vaccine design.

关键词： Helicobacter pylori Lpp20 CD4+ T cell immunodominant epitope

The role of Notch signaling in the macrophage during Helicobacter pylori infection

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The role of Notch signaling in the macrophage during Helicob...

第十三届全国免疫学学术大会

作者： Junjie Wen jianbin ye Danlin Wu JInling Xie Bingxin Hu Meiqun Luo Yanqing Lin Yunshan Ning Yan Li Southern Medical University

Background: Recent data have indicated that the Notch signal pathway is an important bridge between antigen-presenting cells(APCs) and T cell communication circuits and plays a central role in driving the immune system to overcome ***, the role of Notch signaling in the macrophages during Helicobacter pylori(***) infection remains ***: To test whether *** infection regulates the expression of Notch molecules in ***: quantitative real-time PCR was performed and mRNA expression levels of Notch receptors and ligands were evaluated after macrophages were challenged with ***(MOI 10:1 and 50:1).Notch signaling inhibitor GSI, was used to block Notch signaling in the co-culture of macrophage and ***: The expression of Notch receptor Notch3 and Notch ligands(DLL1, DLL4 and Jagged1) were up-regulated significantly following *** challenge macrophages, while the expression of other Notch receptors(Notch1, notch2 and notch4) and ligand(Jagged2) were not observed ***, the Notch3 and the Notch downstream target gene Hes1 were significantly *** up-regulation of ***-induced Notch receptors and ligands was decreased in macrophage after GSI blocked Notch ***: Notch signaling is pivotal in the activation of macrophage during *** infection and further studies should be investigated to develop new therapeutic targets based on Notch signaling.

关键词： Helicobacter pylori Notch signaling macrophage

普速线ZPW-2000A轨道电路调整电压偏高问题的探讨

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铁路通信信号工程技术 2019年第3期16卷 56-60页

作者：叶建斌中国铁路广州局集团有限公司电务部广州510088

国内某些既有普速线ZPW-2000A轨道电路存在调整电压、分路残压过高的现象,严重时会导致占用丢失,影响行车安全。选取几个典型的占用丢失区段,用m a t l a b进行建模仿真计算,分析不同道砟电阻值对轨道电路调整的影响,并提出降低调整电... 详细信息

国内某些既有普速线ZPW-2000A轨道电路存在调整电压、分路残压过高的现象,严重时会导致占用丢失,影响行车安全。选取几个典型的占用丢失区段,用m a t l a b进行建模仿真计算,分析不同道砟电阻值对轨道电路调整的影响,并提出降低调整电压的方法,为后续解决彻底该类问题提供思路。

关键词：普速线ZPW-2000A 调整电压过高道砟电阻

CTCS2+ATO系统列车门与站台门联动原理和优化方案

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铁路通信信号工程技术 2019年第8期16卷 5-8,14页

作者：叶建斌中国铁路广州局集团有限公司电务部

详细介绍CTCS2+ATO系统与站台门接口实现原理和功能,深度分析列车门和站台门联动原理,探讨并分析联动失败的主要成因和优化方案。同时,介绍站台门系统主要故障类型,为后续加强相关子系统的管理和维护介绍相关的经验。

关键词：城际铁路 CTCS-2 ATO 站台门联动失效分析