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The relations between the metallicity and orbital parameters of Galactic disk stars

Science China(Physics,Mechanics & Astronomy) 2010年第7期53卷 1348-1356页

作者： LI Ji sun jianan FU RuiJuan College of Physics Science&Information Engineering Hebei Normal UniversityShijiazhuang 050016China

The iron abundance gradient in the Galactic disk provides fundamental constraints on the chemical evolution of this important component of the ***,there are still some arguments about the shape of the iron abundance gradient across the Galactic stellar *** provide quantitative constraints on these trends,we constructed an unbiased stellar sample from the common objects of two large *** sample consists of 4004 disk stars in the solar neighborhood with the range of metallicity [Fe/H] from ^-1.0 to ~0.5,including 3855 thin disk stars and 146 thick disk *** range of their mean Galactocentric distances R m is from ~4 to ~11 kpc,and the range of their maximum vertical distance from the Galactic Plane Z max is from 0 to ~3.5 *** relations of the stellar metallicity [Fe/H] with the orbital eccentricity e,and [Fe/H] with R m,as well as [Fe/H] with Z max are investigated in *** results showed that:(1) The orbital eccentricity of disk stars decreases with increasing metallicity,while this trend becomes flat for stars with [Fe/H]>-0.2.(2) For thin disk,there is a clear radial iron gradient of-0.051±0.005 dex/***,this metallicity gradient is discontinuous once we split the sample into the inner(Rm<8kpc) and outer disk(Rm≥8 kpc):a slope of about 0 dex/kpc is present in the inner disk and a slope of-0.12 dex/kpc is in the outer *** the thick disk,there is no trend of [Fe/H] with R m.(3) The vertical gradient of iron abundance is-0.146 dex/kpc and-0.251 dex/kpc for the thick and thin disks,***,the vertical abundance gradient shows a steeper trend with the evolution of the *** results confirm the collapse mechanism associated with the formation and evolution of the Galactic disk.

关键词： Galaxy Galactic disk star orbital eccentricity abundance gradient

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Biodegradation of Crystalline Chitin:A Review of Recent Advancement,Challenges,and Future Study Directions

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Journal of Ocean University of China 2024年第5期23卷 1319-1328页

作者： SONG Jianlin SU Haipeng sun jianan MAO Xiangzhao Sanya Institute of Oceanography Ocean University of ChinaSanya 572000China State Key Laboratory of Marine Food Processing and Safety Control College of Food Science and EngineeringOcean University of ChinaQingdao 266404China Qingdao Key Laboratory of Food Biotechnology Qingdao 266404China Key Laboratory of Biological Processing of Aquatic Products China National Light IndustryQingdao 266404China Laboratory for Marine Drugs and Bioproducts of Laoshan Laboratory Qingdao 266237China

Chitin is the second most abundant renewable polysaccharide on *** degradation of chitin into soluble and bioactive N-acetyl chitooligosaccharides(NCOSs)and N-acetyl-D-glucosamine(GlcNAc)has emerged as a pivotal step in the efficient and sustainable utilization of chitin ***,because of its dense structure,high crystallinity,and poor solubility,chitin typically needs pretreatment via chemical,physical,and other methods before enzymatic conversion to enhance the accessibility between substrates and enzyme ***,there has been considerable interest in exploring the direct biological degradation of crystalline chitin as a cost-effective and environment-friendly *** review endeavors to present several biological methods for the direct degradation of *** primarily focused on the importance of chitinase containing chitin-binding domain(CBD).Additionally,various modification strategies for increasing the degradation efficiency of crystalline chitin were ***,the review systematically elucidated critical components of multi-enzyme catalytic systems,highlighting their potential for chitin ***,the application of microorganisms in the degradation of crystalline chitin was also *** insights in this review contribute to the explorations and investigations of enzymatic and microbial approaches for the direct degradation of crystalline chitin,thereby fostering advancements in biomass conversion.

关键词： crystalline chitin chitinase biological degradation engineering microorganisms

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Recovery of Chitin and Protein from Shrimp Head Waste by Endogenous Enzyme Autolysis and Fermentation

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Journal of Ocean University of China 2019年第3期18卷 719-726页

作者： GUO Na sun jianan ZHANG Zhaohui MAO Xiangzhao College of Food Science and Engineering Ocean University of China Qingdao 266003 China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and Technology Qingdao 266237 China

The industrial processing of shrimp produces massive quantities of solid waste that is a notable source of animal protein, chitin, carotenoids, and other bioactive compounds that are not appropriately utilized. In the present study, chitin and protein extraction from shrimp head with autolysis and fermentation using Bacillus licheniformis were investigated. The results showed that when shrimp heads were autolyzed with a natural pH at 50℃ for 4 h, the total amino acid nitrogen in the supernatant was 5.01 mg mL^-1. Then, when a 50%(v/m) inoculum of the hydrolysate was incubated at 60℃ for 10 h, a deproteinization rate of 88.3% could be obtained. The fermented supernatant was processed into a dry protein powder, while the residues were demineralized by 10% citric acid for chitin. The recovered protein powder contained 5.5% moisture, 11.5% ash, and 66.7% protein, while the chitin contained 3.5% moisture, 2.1% ash, and 3.1% protein. In addition, amino acids, minerals, heavy metals, the degree of acetylation, microstructure, and Fourier-transform infrared(FT-IR) spectroscopy results were analyzed. Furthermore, the statistics of the large scale trial after treatment with 20 kg of shrimp heads were analyzed. Thus, this work made the shrimp waste utilization environmentally sound and valuable.

关键词： shrimp head waste endogenous enzyme autolysis fermentation chitin

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Biotransformation of Shrimp Wastes by Bacillus subtilis OKF04 and Evaluation of Growth Promoting Effect in Crop Planting

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Journal of Ocean University of China 2023年第5期22卷 1383-1392页

作者： HU Zelin PAN Zhaoyang ZHAO Tianyu WANG Yongzhen sun jianan MAO Xiangzhao College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

In this study,we proposed a reliable and sustainable technique for the clean utilization of shrimp wastes,which can yield a solid inoculant of Bacillus subtilis OKF04 containing micronutrients at low cost without the risk of *** of the culture conditions revealed that the head of shrimp Litopenaus vannamei and the wheat bran acted as suitable substrates for the growth of *** *** 60%initial moisture content,30℃culture temperature,and 5%inoculation amount,followed by 48 hours of fermentation and 0.5%soluble starch added during the drying process(50℃for 6h),a solid *** OKF04 inoculant with a spore amount of 2.4×10^(10)CFU g^(-1)and a high amino acid content was *** solid *** OKF04 inoculant was applied to cultivate pakchoi under pot *** the result,of adding to,the size of stems and leaves,nutritional composition,and physiological activity of pakchoi were significantly(P<0.05)enhanced by solid *** OKF04 *** OKF04 also significantly(P<0.05)increased the soil’s nutrient content and improved its microbial ***,pakchoi cultivated with a low dose of solid *** OKF04 inoculant(0.05 g kg^(-1)soil)resulted in the best *** study provides a new method for the preparation of microbial inoculants with solid waste shrimp heads.

关键词： shrimp wastes Bacillus subtilis OKF04 inoculant solid state fermentation crop growth promotion

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A comprehensive review of natural products with anti-hypoxic activity

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Chinese Journal of Natural Medicines 2023年第7期21卷 499-515页

作者： LIU Juncai GE Zhen JIANG Xiao ZHANG Jingjing sun jianan MAO Xiangzhao College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Medical College Qingdao Binhai UniversityQingdao 266555China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Natural products exhibit substantial impacts in the field of anti-hypoxic *** can cause altitude sickness and other negative effect on the ***,coma,exhaustion,vomiting and,in severe cases,death are some of the clinical ***,hypoxia is no longer just a concern in plateau regions;it is also one of the issues that can not be ignored by urban *** review covered polysaccharides,alkaloids,saponins,flavonoids,peptides and traditional Chinese compound prescriptions as natural products to protect against *** active ingredients,effectiveness and mechanisms were *** related anti-hypoxic mechanisms involve increasing the hemoglobin(HB)content,glycogen content and adenosine triphosphate(ATP)content,removing excessive reactive oxygen species(ROS),reducing lipid peroxidation,regulating the levels of related enzymes in cells,protecting the structural and functional integrity of the mitochondria and regulating the expression of apoptosis-related *** comprehensive summaries are beneficial to anti-hypoxic research and provide useful information for the development of anti-hypoxic products.

关键词： Natural products Anti-hypoxia Mechanism Activity

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Characterization of Turbot(Scophthalmus maximus) Skin and the Extracted Acid-Soluble Collagen

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Journal of Ocean University of China 2019年第3期18卷 687-692页

作者： sun jianan ZHANG Jingjing ZHAO Dandan XUE Changhu LIU Zhen MAO Xiangzhao College of Food Science and Engineering Ocean University of China Qingdao 266003 China Medical College Qingdao Binhai University Qingdao 266555 China

The biochemical composition of the turbot skin was investigated. The moisture level of the skin was found to be 51.4%. Based on dry matter content, there were relatively high protein(82.1%) and lipid(13.1%) concentrations in the turbot skin. Mineral element analysis revealed that the turbot skin had high Ca content(2069.0 mg kg^-1), and the concentrations of toxic heavy metals Hg and Pb were less than 0.005 mg kg^-1, which indicates that the turbot skin is a safe resource for collagen production. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE) analysis showed that acid-soluble collagen(ASC) extracted from the turbot skin was type-I collagen. The imino acid content of the ASC was 241.6 per 1000 amino acids, which suggests a relatively high denaturation temperature. The Fourier transform infrared(FTIR) spectra of ASC reflected a highly stable structure, and the measured denaturation temperature of ASC was 29.5℃, which is higher than those from many temperate fishes. ASC was the most soluble at pH 4.0, and when the pH values were below or above 4.0, the solubility decreased rapidly. The ASC exhibited a relatively high solubility when NaCl concentration was lower than 2%. These results show that turbo skin can be employed as a source for producing collagen with high quality.

关键词： turbot skin acid-soluble collagen byproduct extraction collagen

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Applying Both Chemical Liquefaction and Enzymatic Catalysis Can Increase Production of Agaro-Oligosaccharides from Agarose

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Journal of Ocean University of China 2020年第6期19卷 1371-1377页

作者： JIANG Chengcheng LIU Zhen LIU Jie sun jianan XU Jiachao LI Laihao MAO Xiangzhao College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology Qingdao 266237China Key Laboratory of Aquatic Product Processing Ministry of AgricultureSouth China Sea Fisheries Research InstituteChinese Academy of Fishery ScienceGuangzhou 510300China

Red algae represents an important marine *** high-value utilization of red algae is the production of agarooligosaccharides which have many positive biological ***,the lack of an efficient production route seriously limits the application of *** this study,we established a green route that combines chemical liquefaction and enzymatic catalysis for the efficient production of agaro-oligosaccharides,and we used the production of neoagarotetraose(NA4)as an ***(150 g L^−1)liquefaction by citric acid was controlled with respect to two targets:a 100%liquefaction rate and a high average degree of polymerization(>4)of the liquesced agaro-oligosaccharides,which were then catalyzed byβ-agarase into an oligosaccharides mixture with a high concentration of NA4(30.8 g L^−1)in a 1-L reaction *** purification,we obtained 25.5 g of NA4 with a purity of 92%.This work establishes an easy route for the efficient production of pure agaro-oligosaccharides from agarose.

关键词： neoagarotetraose agarose agarase marine polysaccharide expression

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Copper(Ⅱ)-catalyzed enantioselective conjugate addition of nitro esters to 2-enoyl-pyridine N-oxides

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Chinese Chemical Letters 2019年第3期30卷 569-572页

作者： jianan sun Yanan Li Yang Gui Youguo Xu Zhenggen Zha Zhiyong Wang Hefei National Laboratory for Physical Sciences at Microscale CAS Key Laboratory of Soft Matter Chemistry & Center for Excellence in Molecular Synthesis of Chinese Academy of Sciences Collaborative Innovation Center of Suzhou Nano Science and Technology & School of Chemistry and Materials ScienceUniversity of Science and Technology of China

A highly enantioselective Michael addition of nitro esters to 2-enoyl-pyridine N-oxides was developed by using chiral copper catalysts. The Michael addition products can be obtained in high yields and with up to 96% ee. Moreover, asymmetric Michael addition product of nitromethane to 2-enoyl-pyridine N-oxides can be obtained in one step. An analogue of nicotine, dihydro-2H-pyrrol 4b was synthesized with this developed method.

关键词： Michael reaction Asymmetric catalysis Copper catalysis Nitro esters 2-Enoyl-pyridine N-oxides

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New possible silver lining for pancreatic cancer therapy:Hydrogen sulfide and its donors

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Acta Pharmaceutica Sinica B 2021年第5期11卷 1148-1157页

作者： Xu Hua Yan Xiao jianan sun Bao Ji Shanshan Luo Bo Wu Chao Zheng Peng Wang Fanxing Xu Keguang Cheng Huiming Hua Dahong Li Key Laboratory of Structure-Based Drug Design&Discovery Ministry of EducationSchool of Traditional Chinese Materia MedicaShenyang Pharmaceutical UniversityShenyang 110016China School of Pharmaceutical Engineering Shenyang Pharmaceutical UniversityShenyang 110016China Key Laboratory of Cardiovascular and Cerebrovascular Medicine Nanjing Medical UniversityNanjing 211166China Molecular Imaging Laboratory MGH/MIT/HMS Athinoula A.Martinos Center for Biomedical ImagingDepartment of RadiologyMassachusetts General Hospital/Harvard Medical SchoolCharlestownMA 02129USA PET Center Department of Radiology and Biomedical ImagingYale University School of MedicineNew HavenCT 06520USA Department of Biomedical Engineering School of EngineeringChina Pharmaceutical UniversityNanjing 210009China Wuya College of Innovation Shenyang Pharmaceutical UniversityShenyang 110016China State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources School of Chemistry and PharmacyGuangxi Normal UniversityGuilin 541004China

As one of the most lethal diseases,pancreatic cancer shows a dismal overall prognosis and high resistance to most treatment ***,pancreatic cancer escapes early detection during the curable period because early symptoms rarely emerge and specific markers for this disease have not been *** combinations of new drugs,multimodal therapies,and adjuvants prolong survival,most patients still relapse after surgery and eventually ***,the search for more effective treatments for pancreatic cancer is highly relevant and *** a newly re-discovered mediator of gasotransmission,hydrogen sulfide(H2S)undertakes essential functions,encompassing various signaling complexes that occupy key processes in human *** evidence indicates that H2S exhibits bimodal modulation of cancer ***,endogenous or low levels of exogenous H2S are thought to promote cancer,whereas high doses of exogenous H2S suppress tumor ***,inhibition of endogenous H2S production also suppresses tumor ***,H2S biosynthesis inhibitors and H2S supplementation(H2S donors)are two distinct strategies for the treatment of ***,modulation of endogenous H2S on pancreatic cancer has not been studied so ***,H2S donors and their derivatives have been extensively studied as potential therapeutic agents for pancreatic cancer therapy by inhibiting cell proliferation,inducing apoptosis,arresting cell cycle,and suppressing invasion and migration through exploiting multiple signaling *** far as we know,there is no review of the effects of H2S donors on pancreatic *** on these concerns,the therapeutic effects of some H2S donors and NO-H2S dual donors on pancreatic cancer were summarized in this *** H2S donors may be promising compounds for pancreatic cancer treatment.

关键词： Pancreatic cancer Hydrogen sulfide donor Sulfur-containing compound Cell proliferation Antitumor effect Signaling pathway

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Triggering the biocontrol of Botrytis cinerea by Trichoderma harzianum through inhibition of pathogenicity and virulence related proteins

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Frontiers of Agricultural Science and Engineering 2018年第2期5卷 271-279页

作者： Kandasamy SARAVANAKUMAR Zhixiang LU Hai XIA Meng WANG jianan sun Shaoqing WANG Qiang-qiang WANG Yaqian LI Jie CHEN School of Agriculture and Biology/State Key Laboratory of Microbial Metabolism/Key Laboratory of Urban Agriculture (South) of the Ministry of Agriculture Shanghai Jiao Tong University

This study reports a strain of Trichoderma harzianum CCTCC-SBW0162 with potential to enhance biocontrol activity against gray mold pathogen, Botrytis cinerea, and with a pivotal role in tomato(Solanum esculentum) plant growth enhancement. A total of 254 Trichoderma isolates were screened by in vitro antagonistic assay. Of these, 10 were selected for greenhouse experiments based on their greater inhibition of B. *** in vitro antagonistic assay and greenhouse experiments indicated that T. harzianum CCTCC-SBW0162 gave the highest inhibition rate(90.6%) and disease reduction(80.7%). Also, to study the possible mechanism associated with antifungal activity of CCTCC-SBW0162 against B. cinerea, molecular docking was used to assess the interactions between CCTCC-SBW0162-derived metabolites, and pathogencity and virulence related proteins of B. cinerea. The molecular docking results indicated that the combination of harzianopyridone,harzianolide and anthraquinone C derived from CCTCCSBW0162 could synergistically improve antifungal activity against B. cinerea through the inhibition/modification of pathogenicity and virulence related ***, this computerized modeling work emphasized the need for further study in the laboratory to confirm the effect T. harzianum-derived metabolites against the proteins of B. cinerea and their interactions.

关键词： anthraquinone Botrytis cinerea harzianolide harzianopyridone molecular docking Trichoderma harzianum

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