检索结果-南通市图书馆

GSDMB N-terminal assembles in plasma membrane to execute pyroptotic cell death

在线全文

学校读者我要写书评

暂无评论

Genes & Diseases 2022年第6期9卷 1405-1407页

作者： Wenbin Gong Peizhao Liu Juanhan Liu Yangguang Li Tao Zheng Xiuwen Wu Yun Zhao jianan ren School of Medicine Southeast UniversityResearch Institute of General SurgeryJinling HospitalNanjingJiangsu 210002PR China Medical School of Nanjing University NanjingJiangsu 210093PR China BenQ Medical Center The Affiliated BenQ Hospital of Nanjing Medical UniversityNanjingJiangsu 210019PR China

Human genome encodes six paralogous gasdermin genes:GSDMA,GSDMB,GSDMC,GSDMD,GSDME and DFNB59.1 Proteolytic cleavage of these gasdermin proteins liberates an N-terminal(NT)fragment from autoinhibition,which assembles in membrane to form pores and execute pyroptotic cell death in general.1 In contrast to other gasdermins,gasdermin B(GSDMB)is the only gasdermin gene that has not been identified in rodents.Zhou et al first shed light on the molecular mechanism by which cytotoxic lymphocyte-derived granzyme A(GZMA)cleaves GSDMB to execute pyroptosis in GSDMB-positive cells,especially in cancer cells.2 In this issue of Cell,Hansen et al reported a dynamic host pathogen S.flexneri prevents GSDMB-mediated lysis by secreting IpaH7.8,which targets and ubiquitinates GSDMB for 26S proteasome destruction.3 They showed that GSDMB implements bacteriocidic ability by recognition of the phospholipids on Gram-negative bacterial membranes rather than lysing host cells.Although their experimental design and data are clearly presented and straightforward,there are still some doubts to be clarified.

关键词： death execute GSD

Microfluidic intestinal organoid-on-a-chip uncovers therapeutic targets by recapitulating oxygen dynamics of intestinal IR injury

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第12期30卷 1-14页

作者： Jinjian Huang Ziyan Xu Jiao Jiao Zongan Li Sicheng Li Ye Liu Ze Li Guiwen Qu Jie Wu Yun Zhao Kang Chen Jieshou Li Yichang Pan Xiuwen Wu jianan ren Research Institute of General Surgery Jinling HospitalSchool of MedicineSoutheast UniversityNanjing210009China School of Medicine Nanjing UniversityNanjing210093China Department of Rehabilitation The First Affiliated Hospital of Nanjing Medical UniversityNanjing210029China Jiangsu Key Laboratory of 3D Printing Equipment and Manufacturing NARI School of Electrical and Automation EngineeringNanjing Normal UniversityNanjing210042China General Clinical Research Center Nanjing Benq HospitalNanjing Medical UniversityNanjing210019China State Key Laboratory of Materials-Oriented Chemical Engineering College of Chemical EngineeringNanjing Tech UniversityNanjing211816China

Increasing evidence demonstrates that mammals have different reactions to hypoxia with varied oxygen dynamic patterns.It takes~24 h for tri-gas incubator to achieve steady cell hypoxia,which fails to recapitulate ultrafast oxygen dynamics of intestinal ischemia/reperfusion(IR)injury.Inspired from the structure of native intestinal villi,we engineered an intestinal organoid chip embedded with engineered artificial microvessels based on coaxial microfluidic technology by using pH-responsive ZIF-8/sodium alginate scaffold.The chip was featured on:(i)eight times the oxygen exchange efficiency compared with the conventional device,tri-gas incubator,(ii)implantation of intestinal organoid reproducing all types of intestinal epithelial cells,and(iii)bio-responsiveness to hypoxia and reoxygenation(HR)by presenting metabolism disorder,inflammatory reaction,and cell apoptosis.Strikingly,it was found for the first time that Olfactomedin 4(Olfm4)was the most significantly downregulated gene under a rapid HR condition by sequencing the RNA from the organoids.Mechanistically,OLFM4 played protective functions on HR-induced cell inflammation and tissue damage by inhibiting the NF-kappa B signaling activation,thus it could be used as a therapeutic target.Altogether,this study overcomes the issue of mismatched oxygen dynamics between in vitro and in vivo,and sets an example of next-generation multisysteminteractive organoid chip for finding precise therapeutic targets of IR injury.

关键词： Ionic barrier of ZIF-8 Microfluidics Organoid-on-a-chip Oxygen dynamics Olfm4

Microfluidic generation of egg-derived protein microcarriers for 3D cell culture and drug delivery

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2017年第18期62卷 1283-1290页

作者： Yuxiao Liu Qian Huang Jie Wang Fanfan Fu jianan ren Yuanjin Zhao State Key Laboratory of Bioelectronics School of Biological Science and Medical Engineering Southeast University Department of General Surgery Jinling Hospital Medical School of Nanjing University

Microcarriers have a demonstrated value for biomedical applications,in particular for drug delivery and three-dimensional cell culture.Attempts to develop this technique tend to focus on the fabrication of functional microparticles by using convenient methods with innovative but accessible materials.Inspired by the process of boiling eggs in everyday life,which causes the solidification of egg proteins,we present a new microfluidic‘‘cooking"approach for the generation of egg-derived microcarriers for cell culture and drug delivery.As the egg emulsion droplets are formed with exquisite precision during the microfluidic emulsification,the resultant egg microcarriers present highly monodisperse and uniform morphologies at the size range of hundred microns to one millimeter.Benefiting from the excellent biocompatibility of the egg protein components,the obtained microcarriers showed good performances of cell adherence and growth.In addition,after a freezing treatment,the egg microcarriers were shown to have interconnected porous structures throughout their whole sphere,could absorb and load different kinds of drugs or other active molecules,and work as microcarrier-based delivery systems.These features point to the potential value of the microfluidic egg microcarriers in biomedicine.

关键词：体细胞培养药物输送微载体微流控芯片三维卵蛋白生物医学应用潜在价值

Biomimetic intestinal barrier based on microfluidic encapsulated sucralfate microcapsules

在线全文

学校读者我要写书评

暂无评论

Science Bulletin 2019年第19期64卷 1418-1425页

作者： Cheng Zhao Yunru Yu Xiaoxuan Zhang Xiuwen Wu jianan ren Yuanjin Zhao Department of General Surgery Jinling HospitalMedical School of Nanjing UniversityNanjing210002China State Key Laboratory of Bioelectronics School of Biological Science and Medical EngineeringSoutheast UniversityNanjing210096China

Intestinal barriers play an important role in preventing intestinally derived diseases,and maintaining their function is a promising approach to prevent and treat those diseases.Here,inspired by the protection effect of intestinal barriers in live organisms and the mucosa adhesive property of sucralfate,we present a biomimetic intestinal barrier based on microfluidic encapsulated sucralfate microcapsules.Benefiting from the flexible selectivity and precise control of microfluidic electrospray flows,the generated microcapsules were imparted with stomach-tolerant dietary-fibre shells and controllable released sucralfate cores,both of which could contribute to forming a continuous biomimetic intestinal barrier on the intestine.Through in vitro adhesive study,in vivo computed tomography(CT)imaging and in vivo imaging system(IVIS)methods,we have demonstrated that the microcapsule-derived biomimetic intestinal barrier can effectively block food fermentation in the gut,reduce generation of fat,decrease disease risk indexes,and prevent obesity.These features make the microfluidic encapsulated sucralfate microcapsules and their resultant biomimetic intestinal barrier an approach for treating obesity and other intestinal diseases.

关键词： Biomimetic Microfluidics Microcapsule Intestinal barrier Gastrointestinal disease

Applications of four-dimensional printing in emerging directions:Review and prospects

在线全文

学校读者我要写书评

暂无评论

Journal of Materials Science & Technology 2021年第32期91卷 105-120页

作者： Jinjian Huang Shaojun Xia Zongan Li Xiuwen Wu jianan ren D Printing Group PLA Key Laboratory of Trauma and Surgical InfectionsJinling HospitalSchool of MedicineSoutheast UniversityNanjing 210009China Jiangsu Key Laboratory of 3D Printing Equipment and Manufacturing NARI School of Electrical and Automation EngineeringNanjing Normal UniversityNanjing 210042China

Four-dimensional(4 D) printing technology is an extension of three-dimensional(3 D) printing technology that enables a 3 D-printed static structure to dynamically change its shape with time. Therefore, the resulting structure can undergo self-folding/unfolding assisted by some stimuli. This technology has made much initial progress in many industrial fields. Aiming to investigate the in-depth application value of4 D printing, this study reviews the recent research and application breakthroughs of 4 D printing in several emerging directions, including the simulation of plant and animal behaviors, smart tissue scaffolds and biomedical devices, food printing, digitalization of industrial art design, renewable energy, intelligent communication, soft electronics and robots, vehicle optimization, textile customization, and flexible machinery and mechanical structure. Based on the analyses of specific cases and processes, we present the current obstacles to large-scale applications and the future prospects.

关键词： 4D printing Smart materials Product design Emerging directions

基于GaN的开关线性复合高速随动脉冲负载直流变换器

在线全文

学校读者我要写书评

暂无评论

电工技术学报 2024年第6期39卷 1818-1829页

作者：樊靖轩施佳楠徐子梁任小永陈乾宏南京航空航天大学江苏省新能源发电与电能变换重点实验室南京210016

有源相控阵雷达发射机收发组件工作在高频脉冲负载模式,这对其供电电源的负载动态性能提出了很高要求。该文针对高速负载切换的二次DC-DC电源,提出开关线性复合并联结构的脉冲负载变换器及其相应的控制策略。基于器件级环路建模的方法... 详细信息

有源相控阵雷达发射机收发组件工作在高频脉冲负载模式,这对其供电电源的负载动态性能提出了很高要求。该文针对高速负载切换的二次DC-DC电源,提出开关线性复合并联结构的脉冲负载变换器及其相应的控制策略。基于器件级环路建模的方法有效提升了线性稳压电路的环路带宽,使其进行快速功率跟踪,并设计基于GaN的低阻抗交错并联Buck变换器,使其以较快的速度高效地提供主要脉冲功率。针对重复频率50 kHz、峰值功率120 W的高频模拟雷达脉冲负载,该文搭建脉冲电流上升下降时间50 ns以内的脉冲电源原理样机,输出电压跌落小于5%。实验结果表明所提方法能够有效改善脉冲电源的动态性能。

关键词：脉冲电源相控阵雷达氮化镓动态响应

Marine-inspired molecular mimicry generates a drug-free, but immunogenic hydrogel adhesive protecting surgical anastomosis

在线全文

学校读者我要写书评

暂无评论

Bioactive Materials 2021年第3期6卷 770-782页

作者： Jinjian Huang Yungang Jiang Ye Liu Yanhan ren Ziyan Xu Zongan Li Yun Zhao Xiuwen Wu jianan ren PLA Key Laboratory of Trauma and Surgical Infections Research Institute of General SurgeryJinling HospitalSchool of MedicineSoutheast UniversityNanjing210009China Chicago Medical School Rosalind Franklin University of Medicine and ScienceNorth ChicagoIL 60064USA School of Medicine Nanjing UniversityNanjing210093China Jiangsu Key Laboratory of 3D Printing Equipment and Manufacturing NARI School of Electrical and Automation EngineeringNanjing Normal UniversityNanjing210042China Gastrointestinal Unit and Center for the Study of Inflammatory Bowel Disease Massachusetts General HospitalHarvard Medical SchoolBostonMA02114USA

Herein,we report the synthesis of a biomimic hydrogel adhesive that addresses the poor healing of surgical anastomosis.Dopamine-conjugated xanthan gum(Da-g-Xan)is fabricated using deep insights into the molecular similarity between mussels'adhesive and dopamine as well as the structural similarity between barnacle cement proteins and xanthan gum.The hydrogel mimics marine animals’adherence to wet tissue surfaces.Upon applying this adhesive to colonic anastomosis in a rat model,protective effects were shown by significantly improving the bursting pressure.Mechanistically,the architecture of Da-g-Xan hydrogel is maintained by dynamic intermolecular hydrogen bonds that allow the quick release of Da-g-Xan.The free Da-g-Xan can regulate the inflammatory status and induce type 2 macrophage polarization(M2)by specifically interacting with mannose receptors(CD206)revealed by RNA-sequencing and molecular binding assays.Consequently,an appropriate microenvironment for tissue healing is created by the secretion of chemokines and growth factors from M2 macrophages,strengthening the fibroblast migration and proliferation,collagen synthesis and epithelial vascularization.Overall,this study demonstrates an unprecedented strategy for generating an adhesive by synergistic mimicry inspired by two marine animals,and the results show that the Da-g-Xan adhesive augments native tissue regenerative responses,thus enabling enhanced recovery following surgical anastomosis.

关键词： Marine animals Non-covalent hydrogel Xanthan gum Mannose receptors Surgical anastomosis

Damps’role in inflammatory bowel disease:a paradoxical player of mtDNA-STING signaling pathway in gut homeostasis

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science Bulletin 2019年第19期64卷 1396-1398页

作者： Qiongyuan Hu Yanhan ren Dominic ASlade Quan Zhou Xiuwen Wu Jinjian Huang Guosheng Gu Gefei Wang jianan ren Jieshou Li Research Institute of General Surgery Jinling HospitalSchool of MedicineNanjing UniversityNanjing210093China Chicago Medical School Rosalind Franklin UniversityNorth ChicagoIL60064-3095USA Department of Surgery Salford Royal NHS Foundation TrustStott LaneSalfordUK School of Medicine Nanjing UniversityNanjing210029China

The inflammatory bowel disease(IBD),including Crohn’s disease(CD)and ulcerative colitis,are chronic,relapsing immune mediated disorders of the gastrointestinal homeostasis and intestinal inflammation[1].Failure to resolve mucosal inflammation and maintain gut barrier are notable shared clinical challenges in IBD,in particular when they activate immune cells within the gut lamia propria.Clinical trials and animal model studies aiming towards DAMPs have demonstrated that they can be effective therapeutic targets in mucosal inflammation of IBD.

关键词： pro IBD Damps mtDNA

在线全文

学校读者我要写书评

暂无评论

测耙数量对进气道稳态畸变特性测量的影响

空气动力学学报 2024年第1期42卷 26-32,I0001页

作者：王霄沈天荣潘英郭佳男任智博沈阳飞机设计研究所沈阳110035 中国飞行试验研究院西安710089 沈阳发动机研究所沈阳110015

在现代战斗机进气道相关试验中,受低速辅助进气门打开和隐身大S弯管道、大迎角侧滑角飞行姿态等复杂因素影响,进气道流场畸变较大,不同测耙数量的测试结果相差较大。国内外对进气道出口流场稳态畸变的测量及计算有较多研究,但鲜有测耙... 详细信息

在现代战斗机进气道相关试验中,受低速辅助进气门打开和隐身大S弯管道、大迎角侧滑角飞行姿态等复杂因素影响,进气道流场畸变较大,不同测耙数量的测试结果相差较大。国内外对进气道出口流场稳态畸变的测量及计算有较多研究,但鲜有测耙数量对进气道稳态畸变测量影响的全面对比研究。本文采用测耙测量进气道大压力梯度区和低压区并计算其稳态畸变,针对进气道风洞试验、全尺寸进发联合台架试验和进发匹配试飞,对常用的6支耙和8支耙形式测量结果进行对比研究。结果表明,对于形状复杂的战斗机进气道而言,进气道出口流场的测试需要尽可能采用8支测耙的测量方式,才能取得满意的结果。特别是舰载战斗机在舰面环境下,受舰首来流及偏流板反射等因素影响,进气道流场更加复杂,且发动机工作在特殊加力状态,对进发匹配稳定性要求也更加苛刻,更有必要采用8支测耙的测量方式。

关键词：进气道进发匹配试飞测试风洞试验畸变测耙进发相容性