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GSDMB promotes non-canonical pyroptosis by enhancing caspase-4 activity

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Journal of Molecular Cell Biology 2019年第6期11卷 496-508页

作者： Qin Chen Peiliang Shi Yufang Wang Dayuan Zou Xiuwen Wu Dingyu Wang Qiongyuan Hu Yujie Zou Zan Huang jianan Ren Zhaoyu Lin Xiang Gao State Key Laboratory of Pharmaceutical Biotechnology and Nanjing Drum Tower Hospital Model Animal Research Center Nanjing University Nanjing 210061 China Department of Surgery Jinling Hospital Medical School of Nanjing University Nanjing 210061 China Jiangsu Province Key Laboratory of Gastrointestinal Nutrition and Animal Health Nanjing Agriculture University Nanjing 210095 China

Gasdermin B (GSDMB) has been reported to be associated with immune diseases in humans, but the detailed molecular mechanisms remain unsolved. The N-terminus of GSDMB by itself, unlike other gasdermin family proteins, does not induce cell death. Here, we show that GSDMB is highly expressed in the leukocytes of septic shock patients, which is associated with increased release of the gasdermin D (GSDMD) N-terminus. GSDMB expression and the accumulation of the N-terminal fragment of GSDMD are induced by the activation of the non-canonical pyroptosis pathway in a human monocyte cell line. The downregulation of GSDMB alleviates the cleavage of GSDMD and cell death. Consistently, the overexpression of GSDMB promotes GSDMD cleavage, accompanied by increased LDH release. We further found that GSDMB promotes caspase-4 activity, which is required for the cleavage of GSDMD in non-canonical pyroptosis, by directly binding to the CARD domain of caspase-4. Our study reveals a GSDMB-mediated novel regulatory mechanism for non-canonical pyroptosis and suggests a potential new strategy for the treatment of inflammatory diseases.

关键词： GSDMB GSDMD pyroptosis sepsis

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黑白河流域湿地水文蓄调功能量化及对比研究

水利水电技术（中英文） 2024年第6期55卷 44-57页

作者：王逸飞李常斌杨致远吕佳南谢旭红高祖桥申娜张旭阳李美静刘源文兰州大学资源环境学院甘肃兰州730000 兰州大学西部环境教育部重点实验室甘肃兰州730000 甘肃省城乡规划设计研究院甘肃兰州730000

【目的】位于若尔盖高原的黑河和白河同属黄河上游一级支流,在高寒湿地水文对比研究方面颇具代表性。【方法】按水量平衡原理,采用SWAT模型对黑河和白河流域山地系统出流进行模拟,以此作为平原区湿地的横向补给量;结合降水和蒸散发等垂... 详细信息

【目的】位于若尔盖高原的黑河和白河同属黄河上游一级支流,在高寒湿地水文对比研究方面颇具代表性。【方法】按水量平衡原理,采用SWAT模型对黑河和白河流域山地系统出流进行模拟,以此作为平原区湿地的横向补给量;结合降水和蒸散发等垂向水文动态,就两河流域湿地水文过程进行分析;再根据湿地土壤水文物理属性估算其静储量;上述动、静储量相结合,就两河流域湿地水文蓄调的年际变化及其功能进行综合研究。【结果】结果显示:2001—2015年间,黑河流域湿地蓄水累计增持19.48亿m^(3);白河流域湿地蓄水累计减持23.08亿m^(3);黑白河流域湿地静储量分别为20.06亿m^(3)和4.59亿m^(3),泥炭沼泽土静储量最大,永冻土静储量最小;统计期间,湿地蓄调功能指数在黑、白河流域均呈降低趋势,黑河湿地的指数降幅高于白河。【结论】结果表明:SWAT模型在黑、白河流域的适用性良好;研究时段内,黑河流域湿地蓄水总体呈增加状态,白河流域湿地蓄水总体呈减少状态;湿地土壤类型和面积大小是影响静储量的关键因素;黑、白河流域湿地蓄持功能总体发生蜕减。研究可为高寒湿地水文蓄调能力提升暨湿地保护提供方法和数据参考。

关键词：湿地水文蓄调水量平衡 SWAT模型黑白河流域时空变化径流地下水

Multi-UAV Route Re-Generation Method Based on Trajectory Data

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Journal of Shanghai Jiaotong university(Science) 2022年第6期27卷 806-816页

作者： YUAN DONGDONG WANG HANKAI bai JIAQI School of Aerospace Engineering Beijing Institute of TechnologyBeijing 100081China

A large quantity of unmanned aerial vehicle(UAV)trajectory data related to air traffic information has important value in engineering ***,the cost of data and trajectory processing limits the applications,and as the number of UAVs increases rapidly,future UAVs'path data will be very ***,this paper designs a multi-UAV route re-generation method based on trajectory data,which can realize the UAVs'path data compression,de-aggregation,and regeneration *** on the trajectory data,the three-dimensional Douglas-Peucker algorithm is used to compress the trajectory data to reduce the storage *** improved B-spline path smoothing algorithm based on the reversing control point is used to depolymerize and smooth the *** experiments show that the above multi-UAV route re-generation algorithm can obtain a more optimized path while maintaining the important characteristics of the original path.

关键词： unmanned aerial vehicle(UAV) trajectory data route re-generation three-dimensional Douglas-Peucker algorithm improved B-spline path smoothing algorithm reversing control point

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健康成人下颈椎在体三维瞬时运动特点研究

中国修复重建外科杂志 2015年第12期29卷 1494-1499页

作者：李宏达夏群白剑强苗军刘佳男魏冬天津医科大学研究生院天津300070 天津市天津医院脊柱外科天津中医药大学研究生院

目的观察并测定健康成人生理载荷下下颈椎在体三维瞬时运动特点。方法纳入17名无颈椎疾患的健康志愿者男8名,女9名;年龄23~41岁平均26岁。通过CT扫描联合Rhinoceros 4.0造模软件获得下颈椎三维模型,将该模型匹配到双X线透视影像系统（... 详细信息

目的观察并测定健康成人生理载荷下下颈椎在体三维瞬时运动特点。方法纳入17名无颈椎疾患的健康志愿者男8名,女9名;年龄23~41岁平均26岁。通过CT扫描联合Rhinoceros 4.0造模软件获得下颈椎三维模型,将该模型匹配到双X线透视影像系统（dual fluoroscopic imaging system,DFIS）捕获的前屈-后伸、左-右侧弯、左-右旋转体位时颈椎双斜位X线图像上,重现生理载荷下不同活动体位时颈椎三维瞬时运动状态通过在椎体几何中心建立三维坐标系定义X轴位于冠状面并指向左侧,Y轴位于矢状面并指向后侧,Z轴垂直于X-Y平面指向头侧。沿X、Y、Z轴的旋转角度分别定义为屈-伸旋转角度（α）、侧弯旋转角度（β）和左-右旋转角度（γ）,沿X,Y,Z轴的位移分别定义为左-右位移（x）、前-后位移（Y）和上-下位移（z）从而获得健康成人下颈椎在体三维运动6自由度数据。结果屈伸活动：除C_（6、7）节段前-后位移显著小于其余各节段（P〈0.05）外,其余各节段间前-后、左-右、上-下位移比较差异均无统计学意义（P〉0.05）;屈-伸旋转角度在C_（4、5）和C_（5、6）节段最大（P〈0.05）、C_（6、7）节段最小（P〈0.05）左-右旋转角度C_（4,5）节段明显大于C_（5、6）和C_（6、7）节段（P〈0.05）;各节段间侧弯旋转角度比较差异均无统计学意义（P〉0.05）。侧弯活动：除C_（3、4）节段前-后位移显著大于C_（4,5）节段,C_（6、7）节段上-下位移显著小于C_（3、4）和C_（4、5）节段（P〈0.05）外,其余各节段间各项位移间比较差异均无统计学意义（P〉0.05）;除C_（3、4）节段左-右旋转角度显著大于C_（5、6）及C_（6、7）节段（P〈0.05）外其余各节段间各项旋转角度比较差异均无统计学意义（P〉0.05）。旋转活动：除C_（3、4）节段左-右旋转角度显著大于C_（4、5）和C_（6、7）节段（P〈0.05）外,其余各节段间各项位移及旋转角度比较差异均无统计学意义（P〉0.05）。结论健康成人下颈椎在体三维瞬时运动具有不同特点,为进一步了解颈椎的生理活动功能提供了参考。

关键词：下颈椎在体运动双X线透视影像系统三维

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包头市空气污染与气象因素相关性研究

环境与发展 2011年第1期23卷 158-160,171页

作者：黄哲王建安白岚包头市环境科学研究院内蒙古014010

本文以包头市市区2007年和2008年的空气环境质量逐时监测数据和相应的地面逐时气象数据为基础,结合MM5中尺度气象模型拟合的高空气象数据,采用Pearson相关分析法,对空气环境中主要污染物二氧化硫、可吸入颗粒物的浓度与风速、近地面气... 详细信息

本文以包头市市区2007年和2008年的空气环境质量逐时监测数据和相应的地面逐时气象数据为基础,结合MM5中尺度气象模型拟合的高空气象数据,采用Pearson相关分析法,对空气环境中主要污染物二氧化硫、可吸入颗粒物的浓度与风速、近地面气温、降水、气压、相对湿度等气象因素的相关性进行了分析,探讨了逆温、静风等主要气象条件对空气环境中污染物浓度变化的影响,为改善城市空气环境质量提供技术支撑。

关键词：空气污染气象因素相关性

大黄素对大鼠骨髓间充质干细胞增殖及造血因子表达的影响

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南方医科大学学报 2014年第5期34卷 736-739,746页

作者：刘铭超危建安白俊其郭兰欧阳玲玲丘小惠广州中医药大学第二临床医学院广东广州510120

目的探究何首乌中主要成分之一的大黄素对骨髓间充质干细胞(BMSCs)的增殖、细胞周期、细胞凋亡的影响以及对造血相关因子表达的影响。方法采用MTT法检测大黄素对BMSCs增殖作用,流式细胞仪检测大黄素对BMSCs细胞周期与细胞凋亡的影响,并... 详细信息

目的探究何首乌中主要成分之一的大黄素对骨髓间充质干细胞(BMSCs)的增殖、细胞周期、细胞凋亡的影响以及对造血相关因子表达的影响。方法采用MTT法检测大黄素对BMSCs增殖作用,流式细胞仪检测大黄素对BMSCs细胞周期与细胞凋亡的影响,并用RT-PCR方法探究大黄素对造血因子表达的影响。结果大黄素0.1μg/ml、1μg/ml在作用BMSCs 48 h与72 h后增殖率明显增高(P<0.05);大黄素作用48 h后,0.1μg/ml能促使细胞周期的G2/M期增多(P<0.05),1μg/ml使S期增多(P<0.01),G1/G0期降低(P<0.05);大黄素在1μg/ml作用48 h后能有效抑制细胞的凋亡(P<0.01);大黄素0.1μg/ml、1μg/ml在作用BMSCs 48 h后对造血因子TPO的表达有显著提高作用(P<0.05)。结论大黄素具有促进BMSCs增殖作用,并且可能通过对细胞周期与细胞凋亡造成影响达到增殖作用,并能促使BMSCs中造血因子TPO表达的升高。

关键词：大黄素骨髓间充质干细胞增殖周期凋亡造血因子

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中国动画电影产业发展的“彩条屋模式”

艺术评论 2019年第11期 81-87页

作者：屈立丰白宇恒王佳楠西华大学美术与设计学院

近五年来,国产动画电影发展出现了具有浓厚"互联网+"色彩的"彩条屋模式"。"彩条屋模式"取得初步成功首先在于其采取类似于"小米生态链"的平台发展战略,打造以产出优质动画电影产品为核心的动画产业发展模式。其次在于通过高水平动画导... 详细信息

近五年来,国产动画电影发展出现了具有浓厚"互联网+"色彩的"彩条屋模式"。"彩条屋模式"取得初步成功首先在于其采取类似于"小米生态链"的平台发展战略,打造以产出优质动画电影产品为核心的动画产业发展模式。其次在于通过高水平动画导演人才等动画产业生产要素的聚集,促进了国产动画电影产品原创力的提升。另外,"彩条屋模式"的成功还在于其"IP动画电影"的制片路线既能低成本、无障碍地推广大众耳熟能详的IP形象,又能讲述具有强烈现实指涉的故事内容;既保留导演个体的艺术个性,又关心观众的观影体验、心灵需求;既追求艺术价值的品质,又追求商业价值的充分显现。这种模式是光线传媒在"互联网+"时代所探索和寻找到的颇具中国特色的动画产业链打造路径。

关键词：国产动画电影动画电影产业彩条屋模式

Multiplexed imaging of tumor immune microenvironmental markers in locally advanced or metastatic non-small-cell lung cancer characterizes the features of response to PD-1 blockade plus chemotherapy

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Cancer Communications 2022年第12期42卷 1331-1346页

作者： Fengying Wu Tao Jiang Gongyan Chen Yunchao Huang Jianying Zhou Lizhu Lin Jifeng Feng Zhehai Wang Yongqian Shu Jianhua Shi Yi Hu Qiming Wang Ying Cheng Jianhua Chen Xiaoyan Lin Yongsheng Wang jianan Huang Jiuwei Cui Lejie Cao Yunpeng Liu Yiping Zhang Yueyin Pan Jun Zhao LiPing Wang Jianhua Chang Qun Chen Xiubao Ren Wei Zhang Yun Fan Zhiyong He Jian Fang Kangsheng Gu Xiaorong Dong Tao Zhang Wei Shi Jianjun Zou Xuejuan bai Shengxiang Ren Caicun Zhou Department of Thoracic Medical Oncology Shanghai Pulmonary HospitalTongji University School of MedicineShanghai 200433P.R.China Department of Respiratory Medicine Harbin Medical University Cancer HospitalHarbinHeilongjiang 150000P.R.China Department of Thoracic Surgery Oncology Yunnan Cancer Hospital&The Third Affiliated Hospital of Kunming Medical University&Yunnan Cancer CenterKunmingYunnan 650118P.R.China Department of Respiratory Medicine The First Affiliated Hospital of Zhejiang UniversityHangzhouZhejiang 310009P.R.China Department of Oncology Center The First Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdong 510405P.R.China Department of Thoracic Medical Oncology Jiangsu Cancer Hospital&Jiangsu Institute of Cancer Research&The Affiliated Cancer Hospital of Nanjing Medical UniversityNanjingJiangsu 210009P.R.China Department of Respiratory Shandong Cancer Hospital&InstituteJinanShandong 250117P.R.China Department of Oncology Jiangsu Province HospitalNanjingJiangsu 210000P.R.China Department of Medical Oncology Linyi Cancer HospitalLinyiShandong 276000P.R.China Department of Oncology Chinese PLA General HospitalBeijing 100853P.R.China Department of Respiratory Medicine Henan Cancer HospitalZhengzhouHenan 450000P.R.China Department of Thoracic Oncology Jilin Cancer HospitalChangchunJilin 130012P.R.China Department of Medical Oncology-Chest Hunan Cancer HospitalChangshaHunan 410006P.R.China Department of Oncology Fujian Medical University Union HospitalFuzhouFujian 350001P.R.China Department of Thoracic Medical Oncology West China HospitalSichuan UniversityChengduSichuan 610000P.R.China Department of Respiration First Affiliated Hospital of Suzhou UniversitySuzhouJiangsu 215002P.R.China Department of Medical Oncology The First Bethune Hospital of Jilin UniversityChangchunJilin 130021P.R.China Pulmonary and Critical Care Medicine The First Affiliated Hospital University of Science Technology of ChinaHefeiAnhui 230036P.R.China De

Background:Although programmed cell death 1(PD-1)blockade plus chemotherapy can significantly prolong the progression-free survival(PFS)and overall survival(OS)in first-line settings in patients with driver-negative advanced non-small-cell lung cancer(NSCLC),the predictive biomarkers remain ***,we investigated the predictive value of tumor immune microenvironmental marker expression to characterize the response features to PD-1 blockade plus ***:Tumor tissue samples at baseline were prospectively collected from 144 locally advanced or metastatic NSCLC patients without driver gene alterations who received camrelizumab plus chemotherapy or chemotherapy *** immune microenvironmental markers,including PD-1 ligand(PDL1),CD8,CD68,CD4 and forkhead box P3,were assessed using multiplex immunofluorescence(mIF)***-Meier curveswere used to determine treatment outcome differences according to their expression *** profiles were compared between tumors with distinct expression levels of these markers and their ***:Responders had significantly higher CD8/PD-L1(P=0.015)or CD68/PD-L1 co-expression levels(P=0.021)than non-responders in the camrelizumab plus chemotherapy group,while no difference was observed in the chemotherapy *** with high CD8/PD-L1 or CD68/PD-L1 co-expression level was associated with significantly longer PFS(P=0.002,P=0.024;respectively)and OS(P=0.006,P=0.026;respectively)than those with low co-expression in camrelizumab plus chemotherapy *** comparing survival in the camrelizumab plus chemotherapy with chemotherapy by CD8/PD-L1 co-expression stratification,significantly better PFS(P=0.003)and OS(P=0.032)were observed in high co-expression *** predictive value of CD8/PD-L1 and CD68/PD-L1 co-expression remained statistically significant for PFS and OS when adjusting clinicopathological *** the prevalence of TP53 or KRAS mutations was similar bet

关键词： Non-small-cell lung cancer PD-1 CD8 CD68 tumor immune microenvironment

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大豆GmNAC23基因的克隆及特征分析

华北农学报 2019年第1期34卷 46-53页

作者：王萍于月华白玉翠郭坤鹏康嘉楠倪志勇新疆农业大学农学院新疆乌鲁木齐830052

NAC是一类植物特异性转录因子,其在调节非生物和生物胁迫的发育和响应中起重要作用。为了探索大豆相关基因的功能,利用PCR的方法从大豆中克隆了1个NAC基因GmNAC23。该基因c DNA全长1 077 bp,开放阅读框长1 053 bp,编码350个氨基酸,蛋白... 详细信息

NAC是一类植物特异性转录因子,其在调节非生物和生物胁迫的发育和响应中起重要作用。为了探索大豆相关基因的功能,利用PCR的方法从大豆中克隆了1个NAC基因GmNAC23。该基因c DNA全长1 077 bp,开放阅读框长1 053 bp,编码350个氨基酸,蛋白质分子量为39. 483 ku,等电点为7. 08。序列分析表明,GmNAC23基因组包含3个外显子和2个内含子。多序列比对结果表明,GmNAC23蛋白含有1个高度保守的NAM结构域。进化树分析表明,该蛋白与绿豆、木豆、鹰嘴豆的NAC蛋白具有同源关系。转录活性分析结果表明,GmNAC23在SD (Trp-/His-/Ade-)营养型缺陷培养基上生长,说明该基因在酵母体内具有转录激活功能。组织特异性表达模式分析表明,在检测的所有组织中GmNAC23都有表达,在根中表达量最高,在茎顶端分生组织中表达量最低。结果表明,大豆GmNAC23基因具有转录激活功能,并且其在根中大量表达。为进一步研究GmNAC23的生物学功能奠定了基础。

关键词：大豆 GmNAC23 克隆转录激活