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OGG1 inhibition suppresses African swine fever virus replication

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Virologica Sinica 2023年第1期38卷 96-107页

作者： Jie Fan Xinqian Lv Saixia Yang Shuxian Geng Jifei Yang Yaru Zhao Zhonghui Zhang Zhijie Liu Guiquan Guan jianxun luo Qiaoying Zeng Hong Yin Qingli Niu College of Veterinary Medicine Gansu Agricultural UniversityLanzhou730070China African Swine Fever Regional Laboratory of China(Lanzhou) State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou730046China Jiangsu Co-Innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonosis Yangzhou UniversityYangzhou225009China

African swine fever virus(ASFV)is an important pathogen that causes a highly contagious and lethal disease in swine,for which neither a vaccine nor treatment is *** DNA repair enzyme 8-oxoguanine DNA glycosylase 1(OGG1),which excises the oxidative base lesion 8-oxo-7,8-dihydroguanine(8-oxoG),has been linked to the pathogenesis of different diseases associated with viral ***,the role of OGG1-base excision repair(BER)in ASFV infection has been poorly *** study aimed to characterize the alteration of host reactive oxygen species(ROS)and OGG1 and to analyse the role of OGG1 in ASFV *** found that ASFV infection induced high levels and dynamic changes in ROS and 8-oxoG and consistently increased the expression of *** yield,transcription level,and protein synthesis were reduced in ASFV-infected primary alveolar macrophages(PAMs)treated by TH5487 or SU0268 inhibiting *** expression of BER pathway associated proteins of ASFV was also suppressed in OGG1-inhibited ***,OGG1 was found to negatively regulate interferonβ(IFN-β)production during ASFV infection and IFN-βcould be activated by OGG1 inhibition with TH5487 and SU0268,which blocked OGG1 binding to ***,the interaction of OGG1 with viral MGF360-14-L protein could disturb IFN-βproduction to further affect ASFV *** results suggest that OGG1 plays the crucial role in successful viral infection and OGG1 inhibitors SU0268 or TH5487 could be used as antiviral agents for ASFV infection.

关键词： African swine fever virus(ASFV) 8-oxoguanine DNA glycosylase 1(OGG1) 8-oxo-7,8-dihydroguanine(8-oxoG) Inhibitors Antiviral effect

Evolution and molecular epidemiology of foot-and-mouth disease virus in China

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Chinese Science Bulletin 2011年第21期56卷 2191-2201页

作者： BAI XingWen LI PingHua BAO HuiFang LIU ZaiXin LI Dong LU ZengJun CAO YiMei SHANG YouJun SHAO JunJun CHANG HuiYun luo jianxun LIU XiangTao State Key Laboratory of Veterinary Etiological Biology National Foot-and-Mouth Disease Reference LaboratoryEngineering Research Center of Biological Detection of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730046China

Foot-and-mouth disease (FMD),caused by foot-and-mouth disease virus (FMDV),is considered one of the most important viral diseases of cloven-hoofed animals,causing severe economic losses in affected regions of the *** serotypes (A,O,and Asia 1) of FMDV have been identified in China since *** addition,the occurrence of novel subtypes within these serotypes has made the epidemiology of FMDV more complicated over the last few *** this review,we summarize the history and the current epidemiological situation in China,genetic diversity (e.g.,quasispecies dynamics,antigenic heterogeneity,and functional constraints),intertypic recombination,and the evidence for positive selection of different FMDV *** also assess these genetic data to understand the origin,evolution,and transmission of FMDV,the findings of which may be useful in developing control measures for future epidemics.

关键词：分子流行病学病毒性传染病中国口蹄疫病毒演变 FMDV 偶蹄类动物遗传多样性

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磺甲基化蛋白填料的合成及性能研究

中国皮革 2005年第19期34卷 19-21页

作者：罗建勋四川大学皮革化学与工程教育部重点实验室成都610065

以工业明胶为原料,采用磺甲基化技术合成蛋白填料,并对其物化性能如溶解性、pH值等,和磺甲基化蛋白填料的用法进行了研究。试验表明:通过该方法合成的蛋白填料在水中有很好的溶解性能,而且在复鞣填充时使用具有优良的填充性能。填充后... 详细信息

以工业明胶为原料,采用磺甲基化技术合成蛋白填料,并对其物化性能如溶解性、pH值等,和磺甲基化蛋白填料的用法进行了研究。试验表明:通过该方法合成的蛋白填料在水中有很好的溶解性能,而且在复鞣填充时使用具有优良的填充性能。填充后的革手感丰满、柔软且粒面细致,尤其可以显著地改善皮革的松面情况,减小皮革的部位差,改善革的手感,提高成品革的质量与档次。

关键词：明胶磺甲基化蛋白填料合成应用

Molecular Survey and Genetic Identification of Anaplasma phagocytophilum of horses in Zhaosu County of Xinjiang Province, China

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Molecular Survey and Genetic Identification of Anaplasma pha...

中国动物学会寄生虫学专业委员会第十届全国寄生虫学青年工作者学术研讨会

作者： Zhenguo Wang Qingli Niu Jifei Yang jianxun luo Hong Yin Zhijie Liu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu Province Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Science

Human granulocytic anaplasmosis(HGA) is a tick transmitted disease caused by the bacterium Anaplasma phagocytophilium. The presence of the pathogen in various of natural hosts including sheep, goats, cattle as well as ticks has been detected by nucleotide acid-based molecular methods, but not reported in horse in China so far. In this study, we detected for Anaplasma phagocytophilum in 100 horse blood DNA samples from Zhaosu County of Xinjiang Province. A 16 S rRNA based species-specific PCR method was used and a target of 641 bp fragment was amplified. The results showed that the positive rate was 6%.Sequence analysis of the 16 S rRNA gene revealed that only one genotype of A. phagocytophilum presents in the horses. These findings should have raised the public health concerns in the investigated region.

关键词： Anaplasma phagocytophilum 16S rRNA gene Horse Tick-borne disease

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西加云杉优树离体复壮与无性繁殖

四川林业科技 1993年第4期14卷 9-15页

作者：罗建勋四川省林业科学研究院

本文报导西加云杉优树离体复壮与无性繁殖。低温预处理(2℃,1～30天)促进休眠顶芽破裂和自由生长芽形成。C型外植体为最佳接种类型。连续继代培养可改进复壮效应、腋芽和不定芽再生率。目前还未找到有效试管内生根法。组培嫩梢扦插生... 详细信息

本文报导西加云杉优树离体复壮与无性繁殖。低温预处理(2℃,1～30天)促进休眠顶芽破裂和自由生长芽形成。C型外植体为最佳接种类型。连续继代培养可改进复壮效应、腋芽和不定芽再生率。目前还未找到有效试管内生根法。组培嫩梢扦插生(?)率可达75％以上。大量部分复壮组培苗已成功移入温室,经园艺生理和外源植物生长调节物质处(?),可为生产提供大量扦条,实现快速大量繁殖。

关键词：西加云杉离体复壮营养繁殖

In vitro infection of bovine and ovine red blood cells with Theileria annulata schizonts

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In vitro infection of bovine and ovine red blood cells with ...

中国奶业协会第26次繁殖学术年会暨国家肉牛牦牛/奶牛产...

作者： Youquan Li Guiquan Guan Zhijie Liu Jifei Yang Qingli Niu jianxun luo Hong Yin State Key Laboratory of Veterinary Etiological Biology Lanzhou Veterinary Research Institute Chinese Academy of Agricultural Science

Theileria annulata,the causative agents of tropical theileriosis,is protozoan parasites that cause lymphoproliferative diseases of *** vivo,parasitized cells undergo clonal expansion and in?Itrate both lymphoid and non-lymphoid tissues of the infected *** this study,in vitro infection of bovine and ovine red blood cells with Theileria annulata schizonts is firstly carried *** experiments showed that Theileria annulata schizonts can infect bovine red blood cells from cattle 2 and cattle 30 in vitro,and also infect ovine red blood cells from sheep 2 and sheep 82 in ***,*** schizonts can infect both bovine red blood cells and ovine red blood cells in *** proliferation of Theileria annulata schizonts-infected lymphocytes is obviously suppressed by bovine red blood cells and ovine red blood *** schizonts could provide an intriguing model for the study of parasite-host interactions in vitro in the future.

关键词： In vitro infection bovine red blood cells ovine red blood cells Theileria annulata schizonts

Simultaneous detection of piroplasms infections in field ticks by Reverse Line Blotting(RLB)

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Simultaneous detection of piroplasms infections in field tic...

中国畜牧兽医学会兽医寄生虫学分会第十三次学术研讨会

作者： Mirza Omar Abdallah Qingli Niu Guiquan Guan Jifei Yang Ze Chen jianxun luo Hong Yin State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Science Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses

Piroplasmosis is caused by tick-borne haemoprotozoan parasites in the genera of Theileria and *** disease leads to clinical infections in domestic and wild animals causing economic *** of those intracellular apicomplexan parasites are highly pathogenic for sheep/goat,cattle and horse with high mortality and *** reports show the growing risks associated with ticks and tick-borne diseases worldwide due to expanding tick populations,global warming,and increased contacts between human,animals,and *** majority of tick-borne pathogen species have been reported in the vertebrate hosts they infect but less in the *** addition,several tick-borne pathogens with known tick vectors display a broader host range than formerly *** of pathogens in ticks allowed us to distinguish pathogens that infected the ticks before they attached to the hosts from the pathogens sucked by ticks during their blood *** this study,the RLB assay is used to investigate the presence of piroplasms in ticks collected from three provinces in ***-Reverse line blot(RLB) analysis couples a universal PCR amplification with the specific hybridization with multiple probes,thus increasing sensitivity and allowing for the simultaneous detection of multiple *** specific probes of 16 piroplasms were designed on the basis of hypervariable region 4(V4 region) of 18 S rRNA *** PCR was first carried out to amplify the V4 region of 18 S gene using a pair of universal primers specific for all members in the genera of Theileria and Babesia;the 18 S rRNA gene fragment was then cloned and sequenced;the positive plasmids were identified by PCR and purified and then used for sensitive *** Babesia and seven Theileria can be detected simultaneously on this *** sensitivity of RLB for each parasite detection is 10%(Babesia sp Hebei),10%(Babesia sp Ningxian),10%(Babesia sp Lintan) 10%(Babesia sp Tianzhu),10%(Babesia sp Xinjiang),10%

关键词： Piroplasms Ticks Reverse line blot(RLB) Detection

Comparative analysis of microRNA profiles between wild and cultured Haemaphysalis longicornis(Acari,Ixodidae) ticks

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Comparative analysis of microRNA profiles between wild and c...

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Jin luo Qiao-yun Ren Wenge Liu Zegong Wu Yangchun Tan Xiaofei Qiu Xiaofeng Xu Hong Yin jianxun luo Guangyuan Liu State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Science

Background:The Haemaphysalis longicornis(***) are of great public health and economic ***(miRNAs) are known with functions of gene regulations at post-transcriptional ***:We herein compared the miRNA profiles of *** wild strain(HLWS) and *** cultured strain(HLCS) by Solexa deep sequencing combined with bioinformatics analysis and stem-loop real-time PCR.A total 15.63 and 15.48 million raw reads were yielded in HLWS and HLCS,*** the data 1517 and 1327 known conserved miRNA were found and 342 miRNAs performed differentially expressed between the two *** prediction showed 36 candidates novel miRNAs were *** exposition the function of novel miRNAs,The Gene Ontology was performed that the target and function prediction revealed a significant set of targets which are related to salivary gland development,pathogen-host interaction and regulation of defense response to pathogen of the wild *** *** cellular component biogenesis,immune system process and response to stimulus etc have high percent in the two tick *** analysis revealed that the percentages of most predicted functions of the miRNA targets were similar,with some taxon specific or taxon enhanced functions,such as different target numbers,specific functions(auxiliary transport protein and electron carrier functions),***:This study provides novel findings showing that miRNA regulation affects the expression of immune genes,indicating a considerable influence of environment-induced stressful stimulation on immune *** in the living environments of ticks can lead to differences in miRNAs between ticks and provide a basis and a convenient means to screen for genes encoding immune factors in ticks.

关键词： MicroRNA(miRNA) Haemaphysalis longicornis Comparative analysis wild culture

MicroRNA-1 Promotes the Development of and Prolongs Engorgement Time in Hyalomma anatolicum anatolicum(Acari:Ixodidae) Ticks

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MicroRNA-1 Promotes the Development of and Prolongs Engorgem...

2021中国畜牧兽医学会兽医寄生虫学分会第16次学术研讨会

作者： Jin luo Qiaoyun Ren Wenge Liu Xiaofei Qiu Gaofeng Zhang Yangchun Tan Runlai Cao Hong Yin jianxun luo Xiangrui Li Guangyuan Liu

MicroRNAs act as mRNA posttranscriptional regulators,playing important roles in cell differentiation,transcriptional regulation,growth and *** this study,microRNA expression profiles of Hyalomma anatolicum anatolicum ticks at different developmental stages were detected by high-throughput sequencing and functionally *** total,2,585,169,1,252,678,1,558,217 and 1,155,283 unique reads were obtained from eggs,larvae,nymphs and adults,respectively,with 42,46,45 and 41 conserved microRNAs in these stages,*** eggs as a control,48,43 and 39 microRNAs were upregulated and 3,10 and 9 downregulated in larvae,nymphs and adults,***-1(miR-1) was expressed in high abundance throughout *** development,with an average of nearly one million transcripts,and it is highly conserved among tick *** real-time PCR(qPCR) showed that miR-1 expression gradually increased with tick development,reaching the highest level at *** tissue expression was detected,with significantly higher levels in the salivary glands and epidermis than in the *** assays showed no significant change in body weight or spawning time or amount between experimental and control groups,but there was a significant difference(p<0.01) in engorgement *** miR-1 inhibition,ticks displayed obvious deformities during later *** more fully explain the microRNA mechanism of action,the miR-1 family was analyzed regarding target gene;members acting on Hsp60 include miR-5,miR-994,miR-969,and miR-1011,which jointly play a ***,microRNAs are critical for normal tick development,and the primary structure of the mature sequence of miR-1 is highly ***,different developmental stages and tissues show different expression patterns,with a certain role in prolonging ***-1,together with other family members,regulates mRNA function and may be used as a molecular marker for species ori

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