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Glucocorticoids Significantly Influence the Transcriptome of Bone Microvascular Endothelial Cells of Human Femoral Head

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Chinese Medical Journal 2015年第14期128卷 1956-1963页

作者： Qing-sheng Yu Wan-Shou Guo Li-Ming Cheng Yu-Feng Lu jian-ying shen Ping Li Department of Orthopedics China-Japan Friendship Hospital Beijing 100029 China Beijing Key Laboratory for Immune-Mediated Inflammatory Diseases Beijing 100029 China Artemisinin Research Center Institute of Chinese Materia Medica China Academy of Chinese Medical Sciences Beijing 100700 China Department of Pharmacology Institute of Clinical Medical Sciences China-Japan Friendship Hospital Beijing 100029 China

Background： Appropriate expression and regulation of the transcriptome, which mainly comprise ofmRNAs and lncRNAs, are important for all biological and cellular processes including the physiological activities of bone microvascular endothelial cells （BMECs）. Through an intricate intraeellular signaling systems, the transcriptome regulates the pharmacological response of the cells. Although studies have elucidated the impact of glucocorticoids （GCs） cell-specific gene expression signatures, it remains necessary to comprehensively characterize the impact of lncRNAs to transcriptional changes. Methods： BMECs were divided into two groups. One was treated with GCs and the other left untreated as a paired control. Differential expression was analyzed with GeneSpring software V12.0 （Agilent, Santa Clara, CA, USA） and hierarchical clustering was conducted using Cluster 3,0 software. The Gene Ontology （GO） analysis was performed with Molecular Annotation System provided by CapitalBio Corporation. Results： Our results highlight the involvement of genes implicated in development, differentiation and apoptosis following GC stimulation. Elucidation of differential gene expression emphasizes the importance of regulatory gene networks induced by GCs. We identified 73 up-regulated and 166 down-regulated long noncoding RNAs, the expression of 107 of which significantly correlated with 172 mRNAs induced by hydrocortisone. Conclusions： Transcriptome analysis of BMECs from human samples was performed to identify specific gene networks induced by GCs. Our results identified complex RNA crosstalk underlying the pathogenesis of steroid-induced necrosis of femoral head.

关键词： Co-expression Network Intracellular Signaling Pathway Microvascular Endothelial Ceils Noncoding RNAs Osteonecrosis

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Overexpression of Tau Rescues Nogo-66-Induced Neurite Outgrowth Inhibition In Vitro

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Neuroscience Bulletin 2016年第6期32卷 577-584页

作者： Yu-Chao Zuo Hong-Lian Li Nan-Xiang Xiong jian-ying shen Yi-Zhi Huang Peng Fu Hong-Yang Zhao Department of Neurosurgery Union Hospital.Tongji Medical CollegeHuazhong University of Science and Technology Department of Neurosurgery The First Affiliated Hospital of Zhengzhou University Department of Anatomy Tongji Medical CollegeHuazhong University of Science and Technology

Abstract Nogo-66 plays a central role in the myelin- mediated inhibition of neurite outgrowth. Tau is a micro- tubule-associated protein involved in microtubule assembly and stabilization. It remains unverified whether tau inter- acts directly with growth factor receptors, or engages in cross-talk with regeneration inhibitors like Nogo-66. Here, we report that plasmid overexpression of tau significantly elevated the protein levels of total tau, phosphorylated tau, and microtubule-affinity regulating kinase （MARK）. Nogo- 66 transiently elevated the total tau protein level and per- sistently reduced the level of p-s262 tau （tau phosphory- lated at serine 262）, whereas it had little influence on the level of p-T205 tau （tau phosphorylated at threonine 205）. Nogo-66 significantly decreased the protein level of MARK. Hymenialdisine, an inhibitor of MARK, signifi- cantly reduced the level of p-S262 tau. Overexpression of tau rescued the Nogo-66-induced inhibition of neurite outgrowth in neuroblastoma cortical neurons. However, 2a （N2a） cells and primary concomitant inhibition ofMARK abolished the rescue of neurite outgrowth by tan in N2a cells. We conclude that dephosphorylation of tau at S262 is able to regulate Nogo-66 signaling, and that overexpression of tau can rescue the Nogo-66-induced inhibition of neurite outgrowth in vitro.

关键词： Neurite outgrowth Myelin inhibitor Microtubule-associated protein Phosphorylation

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新型CDK2-cyclinA2小分子抑制剂的设计、合成及生物学活性研究

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上海交通大学学报（医学版） 2017年第3期37卷 330-336页

作者：卫迎庆张璐胡雨彤张健沈瑛上海交通大学基础医学院上海200025 大连医科大学药学院大连116044

目的·设计、合成新型CDK2-cyclin A2小分子抑制剂并探讨此类抑制剂的构效关系。方法·通过计算机辅助药物设计方法,以CDK2-cyclin A2晶体ATP结合区域为口袋,筛选出先导化合物。根据CDK2-cyclin A2的ATP结合区域特点,设计合成一系列苯... 详细信息

目的·设计、合成新型CDK2-cyclin A2小分子抑制剂并探讨此类抑制剂的构效关系。方法·通过计算机辅助药物设计方法,以CDK2-cyclin A2晶体ATP结合区域为口袋,筛选出先导化合物。根据CDK2-cyclin A2的ATP结合区域特点,设计合成一系列苯磺酰胺类化合物;根据已构建好的体外激酶活性检测体系,对化合物体外活性进行研究。结果·合成了29个新型苯磺酰胺类CDK2-cyclin A2抑制剂,化合物对CDK2-cyclin A2抑制检测结果显示,WZ-026对CDK2-cyclin A2抑制作用的IC_(50)值为3.81μmol/L。结论·综合虚拟筛选、化学合成、生物检测等方法,得到对CDK2-cyclin A2有明显抑制活性的苯环酰胺类化合物WZ-026;初步得到此类小分子化合物与CDK2-cyclin A2的作用模式。

关键词： CDK2-cyclin A2 细胞周期抑制剂构效关系

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Multiple liver metastases of unknown origin:A case report

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World Journal of Gastrointestinal Oncology 2025年第1期17卷 233-238页

作者： ying-Jin Wang Ze-Chuan Liu jian Wang Yin-Mo Yang Department of Hepatobiliary Pancreatic Surgery Peking University First HospitalBeijing 100034China Department of Interventional and Vascular Surgery Peking University First HospitalBeijing 100034China

BACKGROUND The liver is the most common site of digestive system tumor metastasis,but not all liver metastases can be traced back to the primary *** it is unusual,syphilis can impact the liver,manifesting as syphilitic hepatitis with inflammatory nodules,which might be misdiagnosed as *** SUMMARY This case report involves a 46-year-old female who developed right upper abdominal pain and intermittent low fever that persisted for more than three *** definitive diagnosis of a tumor had been made in the past decades,but signs of multiple liver metastases were recognized after a computed tomo-graphy scan without evidence of primary *** positive serological tests for syphilis and a biopsy of the liver nodules,a diagnosis of hepatic syphilis was made and confirmed with follow-up nodule reduction after anti-syphilis *** Clinicians must be aware of the possibility that syphilis can cause hepatic inflam-matory masses,especially when liver metastasis is suspected without evidence of primary lesions.A definitive diagnosis should be established in conjunction with a review of the patient’s medical history for accurate therapeutic intervention.

关键词： Syphilitic hepatitis Liver nodules Liver metastasis Treponema pallidum Case report

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FUTURE TRENDS AND PROSPECTS ON ALLELOPATHY——A NATURAL ALTERNATIVE FOR WEED CONTROL

FUTURE TRENDS AND PROSPECTS ON ALLELOPATHY——A NATURAL ALTE...

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中国第三届植物化感作用学术研讨会、第八届全国杂草科学大会、联合国粮农组织——中国“水稻化感作用论坛”

作者： jian-ying shen Dept.of Environmental Science and Resource,College of Agriculture and Life Science, Shanghai Jiaotong University,Shanghai,200420,China

According to the definition given by the International Allelophathy Society(IAS), allelophathy "studies any process involving secondary metabolites produced by plants,algae, bacteria and fungi that influence the growth and development of agricultural and biological systems".This definition depicts the allelopathic phenomenon as a general defence mechanism

关键词： A NATURAL ALTERNATIVE FOR WEED CONTROL FUTURE TRENDS AND PROSPECTS ON ALLELOPATHY

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Humanin attenuates Alzheimer-like cognitive deficits and pathological changes induced by amyloid β-peptide in rats

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Neuroscience Bulletin 2014年第6期30卷 923-935页

作者： Gao-Shang Chai Dong-Xiao Duan Rong-Hong Ma jian-ying shen Hong-Lian Li Zhi-Wei Ma Yu Luo Lu Wang Xin-Hua Qi Qun Wang jian-Zhi Wang Zelan Wei Darrell D.Mousseau Li Wang Gongping Liu Department of Pathophysiology Key Laboratory of Neurological Diseases of Chinese Ministry of EducationTongji Medical CollegeHuazhong University of Science and Technology Department of Basic Medical Wuxi Medical SchoolJiangnan University Department of Physiology Basic Medical CollegeZhengzhou University Department of Laboratory Medicine Union HospitalHuazhong University of Science and Technology Department of Histology and Embryology Tongji Medical CollegeHuazhong University of Science and Technology Affiliated Hospital of Hebei University of Engineering Department of Psychiatry College of MedicineUniversity of SaskatchewanSaskatoonSaskatchewanS7N 5E5Canada Department of Pathophysiology Henan Medical College

Amyloid β-peptide（Aβ） has been implicated as a key molecule in the neurodegenerative cascades of Alzheimer ’s disease（AD）. Humanin（HN） is a secretory peptide that inhibits the neurotoxicity of Aβ. However, the mechanism（s） by which HN exerts its neuroprotection against Aβ-induced ADlike pathological changes and memory deficits are yet to be completely defined. In the present study,we provided evidence that treatment of rats with HN increases the number of dendritic branches and the density of dendritic spines, and upregulates pre- and post-synaptic protein levels; these effects lead to enhanced long-term potentiation and amelioration of the memory deficits induced by Aβ1-42. HN also attenuated Aβ1-42-induced tau hyperphosphorylation,apparently by inhibiting the phosphorylation of Tyr307 on the inhibitory protein phosphatase-2A（PP2A）catalytic subunit and thereby activating PP2 A. HN also inhibited apoptosis and reduced the oxidativestress induced by Aβ1-42. These findings provide novel mechanisms of action for the ability of HN to protect against Aβ1-42-induced AD-like pathological changes and memory deficits.

关键词： Humanin amyloid-beta Alzheimer’s disease tau apoptosis

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Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats

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World Journal of Gastroenterology 2025年第1期31卷 93-109页

作者： Ping Zhang ying Zhu Pu Chen Tong Zhou Zhe-Yi Han Jun Xiao jian-Feng Ma Wen Ma Peng Zang ying Chen Graduate School Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Key Laboratory of Aerospace Nutrition and Food Engineering China Astronaut Research and Training CenterBeijing 100094China Department of Gastroenterology The Air Force Medical CenterBeijing 100142China Department of Blood Transfusion Air Force Medical CenterBeijing 100142China

BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility *** pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal ***,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal *** cells are the pacemakers of gastrointestinal *** To investigate the effects of Bifidobacterium lactis(***)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit *** The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 *** tail group was given *** BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/***&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and *** fecal flora and mucosal flora of rats in each group were detected by 16S *** Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic *** microgravity reduced the expressions of SCF and c-kit,and increased the express

关键词： Simulated microgravity Rat Intestinal flora Gastrointestinal motility Stem cell factor/c-kit signaling pathway

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低空经济的内涵、特征和运行模式

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新疆师范大学学报（哲学社会科学版） 2025年第1期46卷 108-117,F0002页

作者：沈映春北京航空航天大学公共管理学院北京100083

低空经济作为低空飞行活动与产业融合的新型综合性经济形态,具有空间立体性、区域依赖性、数字生态性、产业融合性以及辐射带动性等特征。党和政府高度重视发展低空经济,将其确定为国家战略性新兴产业,各地加紧布局,发展势头良好。但具... 详细信息

低空经济作为低空飞行活动与产业融合的新型综合性经济形态,具有空间立体性、区域依赖性、数字生态性、产业融合性以及辐射带动性等特征。党和政府高度重视发展低空经济,将其确定为国家战略性新兴产业,各地加紧布局,发展势头良好。但具体实践中仍存在发展低空经济的体制机制不畅、技术创新支持不足、新型基础设施不全、产业领域需不断拓展等问题。针对上述问题,文本依据螺旋理论,提出“政产学研金服用”的低空经济开放式创新生态模式。

关键词：低空经济新质生产力战略性新兴产业开放式创新生态运行模式

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The cGAS-STING-interferon regulatory factor 7 pathway regulates neuroinflammation in Parkinson's disease

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Neural Regeneration Research 2025年第8期20卷 2361-2372页

作者： shengyang Zhou Ting Li Wei Zhang jian Wu Hui Hong Wei Quan Xinyu Qiao Chun Cui Chenmeng Qiao Weijiang Zhao Yanqin shen Laboratory of Neurodegenerative and Neuroinjury Diseases Wuxi Medicine SchoolJiangnan UniversityWuxiJiangsu ProvinceChina

Interferon regulatory factor 7 plays a crucial role in the innate immune ***,whether interferon regulatory factor 7-mediated signaling contributes to Parkinson's disease remains *** we report that interferon regulatory factor 7 is markedly up-regulated in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced mouse model of Parkinson's disease and co-localizes with microglial *** the selective cyclic guanosine monophosphate adenosine monophosphate synthase inhibitor RU.521 and the stimulator of interferon genes inhibitor H151 effectively suppressed interferon regulatory factor 7 activation in BV2 microglia exposed to 1-methyl-4-phenylpyridinium and inhibited transformation of mouse BV2 microglia into the neurotoxic M1 *** addition,si RNA-mediated knockdown of interferon regulatory factor 7 expression in BV2 microglia reduced the expression of inducible nitric oxide synthase,tumor necrosis factorα,CD16,CD32,and CD86 and increased the expression of the anti-inflammatory markers ARG1 and *** together,our findings indicate that the cyclic guanosine monophosphate adenosine monophosphate synthase-stimulator of interferon genes-interferon regulatory factor 7 pathway plays a crucial role in the pathogenesis of Parkinson's disease.

关键词： cyclic guanosine monophosphate adenosine monophosphate synthase H151 interferon regulatory factor 7 M1 phenotype neurodegenerative disease neuroinflammation Parkinson’s disease RU521 STING type I interferon

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纳米晶永磁Pr_2Fe_(14)B微磁学有限元法的模拟计算研究

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物理学报 2003年第3期52卷 718-721页

作者：张宏伟荣传兵张健张绍英沈保根中国科学院物理研究所与凝聚态物理中心磁学国家重点实验室北京100080

根据实验数据 ,构造了接近实际纳米晶永磁Pr2 Fe1 4B的样品 ,用微磁学有限元法进行了模拟计算 .计算结果表明 ,晶界处各向异性的下降会导致矫顽力减小、剩磁值增大 ,而晶界处交换作用常数的减小则会使剩磁值减小、矫顽力增大 .通过对实... 详细信息

根据实验数据 ,构造了接近实际纳米晶永磁Pr2 Fe1 4B的样品 ,用微磁学有限元法进行了模拟计算 .计算结果表明 ,晶界处各向异性的下降会导致矫顽力减小、剩磁值增大 ,而晶界处交换作用常数的减小则会使剩磁值减小、矫顽力增大 .通过对实验样品的模拟研究发现 ,晶界处各向异性和交换作用常数的共同减小能够同时拟合出真实的矫顽力和剩磁值 .模拟计算与实验在退磁曲线形状上的差距则说明模拟还存在不足 .

关键词： Pr2Fe14B 微磁学有限元法模拟计算纳米晶永磁材料磁滞回线矫顽力剩磁

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