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Gender difference in the neuroprotective effect of rat bone marrow mesenchymal cells against hypoxiainduced apoptosis of retinal ganglion cells

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Neural Regeneration Research 2016年第5期11卷 846-853页

作者： Jing yuan jian-xiong yu Eye Center Renmin Hospital of Wuhan University Wuhan Hubei Province China Department of Gastrointestinal Surgery Renmin Hospital of Wuhan University Wuhan Hubei Province China

Bone marrow mesenchymal stem cells can reduce retinal ganglion cell death and effectively prevent vision loss. Previously, we found that during differentiation, female rhesus monkey bone marrow mesenchymal stem cells acquire a higher neurogenic potential compared with male rhesus monkey bone marrow mesenchymal stem cells. This suggests that female bone marrow mesenchymal stem cells have a stronger neuroprotective effect than male bone marrow mesenchymal stem cells. Here, we first isolated and cultured bone marrow mesenchymal stem cells from female and male rats by density gradient centrifugation. Retinal tissue from newborn rats was prepared by enzymatic digestion to obtain primary retinal ganglion cells. Using the transwell system, retinal ganglion cells were co-cultured with bone marrow mesenchymal stem cells under hypoxia. Cell apoptosis was detected by flow cytometry and caspase-3 activity assay. We found a marked increase in apoptotic rate and caspase-3 activity of retinal ganglion cells after 24 hours of hypoxia compared with normoxia. Moreover, apoptotic rate and caspase-3 activity of retinal ganglion cells significantly decreased with both female and male bone marrow mesenchymal stem cell co-culture under hypoxia compared with culture alone, with more significant effects from female bone marrow mesenchymal stem cells. Our results indicate that bone marrow mesenchymal stem cells exert a neuroprotective effect against hypoxia-induced apoptosis of retinal ganglion cells, and also that female cells have greater neuroprotective ability compared with male cells.

关键词： nerve regeneration optic nerve injury bone marrow mesenchymal stem cells retinal ganglion cells neuroprotection hypoxic injury gender difference transwell system co-culture cell apoptosis flow cytometry caspase-3 neural regeneration

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Mechanisms of secondary damage to the thalamic nucleus and substantia nigra in an adult hypertensive rat model following middle cerebral artery occlusion

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Neural Regeneration Research 2010年第11期5卷 853-858页

作者： Li xiong jian yu Songjie Liao Shihui Xing Zhan Zhao Jinsheng Zeng Stroke Center and Department of Neurology First Affiliated Hospital Sun Yat-Sen University Guangzhou 510080 Guangdong ProvinceChina

BACKGROUND： Following ischemia, apoptosis is observed at the ipsilateral ventropostenor thalamic nucleus and substantia nigra, which are distant from, but connected to, the ischemic cerebral cortex, in animals with normotension. However, secondary brain damage in hypertension has not been clearly investigated. OBJECTIVE： The present study determined whether neuronal apoptosis is associated with neuronal loss in the ipsilateral ventroposterior thalamic nucleus and substantia nigra following cortical ischemia in adult hypertensive rats. Results should provide options for determining a time window for anti-apoptotic therapy. DESIGN, TIME AND SETTING： All experimental procedures in this randomized, controlled trial were conducted at the Neurological Laboratory of the First Affiliated Hospital of Sun Yat-sen University of China between October 2006 and July 2008. MATERIALS： Monoclonal primary antibodies specific to mouse anti-rat microtubule-associated protein 2 and glial fibrillary acidic protein were respectively purchased from Sigma Chemical, USA and BD Pharmingen, USA. Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-biotin nick end-labeling （TUNEL） detection kits were purchased from Roche Applied Science, Switzerland and Upstate, USA, respectively. METHODS： A total of 64 male, Sprague Dawiey rats, aged 60-90 days, were equally and randomly divided into middle cerebral artery occlusion and sham surgery groups. Renovascular hypertension was established in both groups by renal artery occlusion. Right distal middle cerebral artery occlusion was performed by electrocoagulation in the middle cerebral artery occlusion group. MAIN OUTCOME MEASURES： Microtubule-associated protein 2 and glial fibrillary acidic protein were detected by immunohistochemistry, and apoptotic cells were observed by TUNEL assay. The stainings were separately detected in the ipsilateral ventroposterior thalamic nucleus and substantia nigra. RESULTS： During the 4 weeks followin

关键词： ventroposterior thalamic nucleus substantia nigra apoptosis rats cerebral infarction neural regeneration

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Postoperative Disc Wedging in Adolescent Idiopathic Thoracolumbar/Lumbar Scoliosis:a Comparison of Anterior and Posterior Approaches

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Chinese Medical Sciences Journal 2010年第3期25卷 156-161页

作者： Bin yu Yi-peng Wang Gui-xing Qiu jian-guo Zhang jian-xiong Shen yu Zhao Shu-gang Li Qi-yi Li Department of Orthopedics Peking Union Medical College HospitalChinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To evaluate the different influences of anterior and posterior correction and fusion approaches upon disc wedging in adolescent idiopathic thoracolumbar/lumbar *** The retrospective study was conducted with the medical records and radiographs of adolescent idiopathic thoracolumbar/lumbar scoliosis patients that underwent anterior(group A) or posterior(group B) correction and fusion surgery from December 1998 to May *** correction of the main curve and changes of the disc wedging were *** Fifty-three patients were included,26 in group A and 27 in group *** mean coronal Cobb angles of the main curve in group A and group B were significantly corrected after surgery(P0.05).The difference between disc wedging at final follow-up and that after surgery was significant in group A(P0.05).Between the two groups,group A had larger disc angles after operation and at final follow-up(P<0.05),and a greater loss of disc angle(P<0.05).Conclusion For adolescent idiopathic thoracolumbar/lumbar scoliosis,posterior approach using all pedicle screws might produce a better result in terms of disc wedging compared with anterior

关键词： adolescent idiopathic scoliosis thoracolumbar/lumbar curve anterior fusion posterior fusion disc wedging

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Changes of Oxidative Stress and Soluble Sugar in Anthers Involve in Rice Pollen Abortion Under Drought Stress

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Agricultural Sciences in China 2011年第7期10卷 1016-1025页

作者： FU Guan-fu SONG jian xiong Jie LI yu-rong CHEN Hui-zhe LE Ming-kai TAO Long-xing State Key Laboratory of Rice Biology China National Rice Research Institute Hangzhou 310006 P.R.China

Two rice maintaining lines with different drought tolerances, viz., Jin 23B （tolerant） and Zhenshan 97B （sensitive）, were used to study the oxidative stress and soluble sugar in rice anthers and pollen viability under drought stress during flowering stage. Higher antioxidant enzyme activities and lower malonaldehyde （MDA） content in rice anthers were observed in Jin 23B than in Zhenshan 97B under drought stress. Further, a great increase in the content of soluble sugar in rice anthers of Jin 23B was observed across the whole drought exposure, while Zhenshan 97B showed significant decrease in soluble sugar during 9-12 d after drought stress （DADS）. Accordingly, a marked decline of pollen fertility and activity, pollen numbers in an anther and pollen numbers on a stigma was observed in Zhenshan 97B, whereas little difference was found in Jin 23B. Thus, we suggest that pollen abortion caused by drought stress may be related with the reciprocity between oxidative stress and soluble sugar content in rice anthers.

关键词： drought stress oxidative stress soluble sugar pollen abortion rice anthers flowering stage

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Efficacy and Safety of Low Molecular Weight Heparin Prophylaxis for Venous Thromboembolism Following Lumbar Decompression Surgery

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Chinese Medical Sciences Journal 2011年第4期26卷 221-226页

作者： Zhi-jian Sun yu Zhao Giu-xing Qiu Yi-peng Wang Xi-sheng Weng Hong Zhao jian-xiong Shen yu jiang Ye Li Xiang Li Department of Orthopedics Peking Union Medical College Hospital Chinese Academy of Medical Sciences ＆ Peking Union Medical College Beijing 100730 China

Objective To evaluate the efficacy and safety of low molecular weight heparin (LMWH) prophylaxis for venous thromboembolism (VTE) after lumbar decompression surgery. Methods Patients at high or the highest risk of VTE who underwent lumbar spine surgery in Peking Union Medical College Hospital from January 2004 to April 2011 were included in the present study. All the patients received a half dose of LMWH 6 hours after surgery followed by a full dose LMWH once per day until discharge. We recorded incidences of deep venous thrombosis (DVT), pulmonary embolism (PE), bleeding complications, and medication side effects. Results Seventy-eight consecutive patients were eligible and enrolled in this study. The mean hospital stat was 8.5±4.5 days. No symptomatic DVT, PE, or major bleeding events were observed. One patient developed wound ecchymosis, another developed wound bleeding, four had mild hepatic aminotransferase level elevation, and one developed a suspicious allergic reaction. Conclusion LMWH may be applied as an effective and safe prophylaxis for VTE in high-risk patients undergoing lumbar decompression surgery.

关键词： venous thromboembolism prophylaxis low molecular weight heparin lumbar decompression surgery

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Primary effect of dual growing rod technique for the treatment of severe scoliosis in young children

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Chinese Medical Journal 2010年第2期123卷 151-155页

作者： LI Qi-yi ZHANG jian-guo QIU Gui-xing WANG Yi-peng SHEN jian-xiong ZHAO yu LI Shu-gang yu Bin WANG Xi WENG Xi-sheng Department of Orthopedic Surgery Peking Union Medical College Hospital Peking Union Medical College Beijing 100730 China

Background Treatment of rapidly progressing scoliosis in young children is a challenge for spine surgeons. Some surgeons had begun to use dual growing rod technique for treatment of rapidly progressing scoliosis in young children and had achieved acceptable results. The aim of this study was to determine the primary results and complications of this new technique in China. Methods Eleven children suffering from rapidly progressing scoliosis were treated with dual growing rod technique between November 2004 and March 2009 at Peking Union Medical College Hospital （PUMCH）. There were 10 females and 1 male in the group with Risser sign of 0 grade. The mean age at initial surgery was 6.1 years （range, 2.1-10.9 years）. Ten patients were diagnosed as congenital scoliosis and 1 patient neuromuscular scoliosis. All the patients had 1-4 lengthening procedures （mean, 1.8 procedures） after the initial surgeries. The radiographic results of all the patients were investigated. Results The coronal Cobb angle of scoliosis improved from （67.64±11.43）% to （34.64±8.26）% after initial surgery with the correction rate observed at （47.15±16.48）%. The coronal trunk shift improved from （2.00±1.73） cm to （1.49±1.31） cm after initial surgery. The T1-S1 height increased from （25.47±6.16） cm to （28.84±5.69） cm after initial surgery. The coronal Cobb angle of scoliosis was （36.82±11.76）% and the coronal trunk shift was （1.11±1.29） cm after the most recent lengthening procedure with the most recent correction rate observed at （44.73±19.43）%. The T1-S1 height was （31.29±4.50） cm after the most recent lengthening procedure with an average T1-S1 length increase of 1.6 （range, 1.0-2.7） cm per year during the lengthening period. The sagittal balance was maintained. Five of the total patients （45.5%） had complications including： hook displacement, pedical screw loosening, and broken rod. We performed revision surgeries with simultaneous lengthening procedures in all 5 cases with satisfactory results.

关键词： scoliosis growing rods operation

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Follow-up study of gallbladder stones in 44 children

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World Journal of Clinical Cases 2024年第18期12卷 3403-3409页

作者： jian-xiong yu Zhi-Zhong jiang yuan-Hao Cai Lin Zhou Bin Cai Department of Pediatric The Firs Affiliated Hospital of Naval Medical UniversityShanghai 20000China

BACKGROUND Cholesterol stones affect a certain subpopulation of *** have been raised on the impact of gallbladder surgery on the growth of children and *** To study the population characteristics,clinical features,treatment,and prognosis of gallstones in *** The clinical data of 44 children with gallstones admitted to The First Affiliated Hospital of Naval Medical University from August 2009 to August 2021 were collected,the children were followed up by telephone to monitor their *** follow-up ended in August *** shortest follow-up time was 2 years and 6 months,whereas the longest was 13 years and 11 *** population characteristics,general clinical characteristics,and treatments were retrospectively *** children were divided according to whether they underwent surgical gallbladder removal into an operation group(n=28)and a non-operation group(n=16),The effects of surgical gallbladder resection on the growth and development of children were *** The male–female ratio in the population was 6:5 and 84.09%of the children had onset in ***,29.55%of the children were overweight or *** study identified 26 cases with metabolic abnormalities,9 with hemolytic anemia,and 4 with choledochal *** the population,68.18%had recurrent symptomatic *** treatment accounted for 63.64%,with laparoscopic cholecystectomy accounting for 71.43%of surgical *** significant differences were observed in symptoms and complications between the surgery and non-surgery ***,no significant differences were found between the two groups in the attainment of genetic height target and the rightward shift of height curve during *** The sex characteristics of gallstones in children were not *** gallstones occurred in adolescents and rarely in young children.A considerable proportion of children have inborn causes,which are of

关键词： Gallstones in children Clinical characteristics Growth and development Operation Clinical features

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Influence of introducing Zr,Ti,Nb and Ce elements on externally solidified crystals and mechanical properties of high-pressure die-casting Al–Si alloy

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International Journal of Minerals,Metallurgy and Materials 2025年第1期32卷 147-153页

作者： Junjie Li Wenbo yu Zhenyu Sun Weichen Zheng Liangwei Zhang Yanling Xue Wenning Liu Shoumei xiong Center of Materials Science and Engineering School of Mechanical and Electronic Control EngineeringBeijing Jiaotong UniversityBeijing 100044China Traffic&Transportation Engineering Central South UniversityChangsha 410075China Shanghai Synchrotron Radiation Facility Shanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201204China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China

High pressure die casting(HPDC)AlSi10Mn Mg alloy castings are widely used in the automobile *** can optimize the mechanical properties of castings through heat treatment,while the release of thermal stress arouses the deformation of large integrated ***,the development of non-heat treatment Al alloys is becoming the hot *** addition,HPDC contains externally solidified crystals(ESCs),which are detrimental to the mechanical properties of *** achieve high strength and toughness of non-heat treatment die-casting Al-Si alloy,we used AlSi9Mn alloy as matrix with the introduction of Zr,Ti,Nb,and *** influences on ESCs and mechanical properties were systematically investigated through three-dimensional reconstruction and thermodynamic *** results reveal that the addition of Ti increased ESCs'size and porosity,while the introduction of Nb refined ESCs and decreased ***,large-sized Al_3(Zr,Ti)phases formed and degraded the mechanical *** introduction of Ce resulted in the poisoning effect and reduced mechanical properties.

关键词： aluminium alloy high-pressure die-casting externally solidified crystals non-heat treatment

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金融科技应用专业课程思政教学探索与实践

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教育教学论坛 2024年第42期 119-122页

作者：熊建宇浙江金融职业学院信息技术学院浙江杭州310018

专业课程开展课程思政教学,有利于将思想教育、政治引领和价值塑造培养融为一体,形成全员、全程、全方位育人协同效应,从而实现立德树人根本任务。通过对“互联网金融营销”课程面向的行业岗位能力素养分析,梳理定位课程教学的知识、能... 详细信息

专业课程开展课程思政教学,有利于将思想教育、政治引领和价值塑造培养融为一体,形成全员、全程、全方位育人协同效应,从而实现立德树人根本任务。通过对“互联网金融营销”课程面向的行业岗位能力素养分析,梳理定位课程教学的知识、能力和思政目标,将爱国主义情怀、互联网思维、文化自信、诚实守信、创新意识、工匠精神等思政元素融入课程教学中,采取多样化教学手段,开展课程思政教学,取得了良好的教学和育人效果,为高职院校金融类专业开展课程思政教育提供借鉴意义。

关键词：金融科技课程思政教学改革

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论银行计算机网络系统的安全防范

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计算机安全 2010年第12期 77-79页

作者：熊建宇浙江金融职业学院浙江杭州310018

随着网络信息技术的发展和电子商务的普及,给银行业的发展带来了巨大的商机,同时也带来了一系列的网络安全问题。从银行业面临的新风险出发,分析了银行计算机网络系统的安全防范措施和手段。

关键词：网上银行网络金融风险防范

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