检索结果-南通市图书馆

mi R-1181 inhibits invasion and proliferation via STAT3 in pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第9期23卷 1594-1601页

作者： Jie Wang xing-Jun Guo You-Ming Ding jian-xin jiang Department of Hepatic-Biliary-Pancreatic Surgery The Affiliated Hospital of Guizhou Medical University Department of Biliary-Pancreatic Surgery Affiliated Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Department of Hepatic-Biliary-Pancreatic Surgery Renmin Hospital of Wuhan University

AIM To examine the role of microRNA 1181 (miR-1181) in invasion and proliferation in pancreatic cancer. METHODS We analyzed the expression of miR-1181 in several pancreatic cancer cell lines and generated stable MIA- PaCa- 2 and PANC-1 cell lines with up-regulated miR-1181 expression using an adenovirus delivery system. We then investigated miR-1181 ' s effect on invasion and proliferation of pancreatic cancer cells by transwell assay, wound healing assay, cell counting kit-8 assay and colony-forming assay, and explored any underlying mechanisms by western bolt. Beyond that, we observed the change of the PANC-1 cell ' s cytoskeleton by immunofluorescence staining. RESULTS Our data showed that miR-1181 was relatively downregulated in pancreatic cancer cell lines compared with normal pancreatic ductal epithelial cells. And miR-1181 inhibited the migration, invasion and proliferation activities of MIA-PaCa-2 and PANC-1 cells. Notably,after over-expressing of miR-1181 in PANC-1 cells, F-actin depolymerized. Immunofluorescence staining shows decreased F-actin and beta-tubulin expression in PANC-1 cells over-expressing miR- 1181 compared with the control cells. Furthermore, we found that over-expressing miR- 1181 inhibited the expression of signal transducer and activator of transcription 3(STAT3) while knocking- down miR-1181 up-regulated the expression of STAT3. Knocking-down miR-1181 promoted the invasion and proliferation of pancreatic cancer cells. And inhibition of STAT3 blocked the promotion effects of knocking- down miR-1181 on proliferation and invasion in pancreatic cancer. CONCLUSION Together our findings suggest that miR-1181 may be involved in pancreatic cancer cell invasion and proliferation by targeting STAT3 and indicate that miR-1181 may be a potential therapeutic agent for pancreatic cancer.

关键词： Pancreatic cancer miR-1181 Proliferation Invasion STAT3

Incidence, casualties and risk characteristics of civilian explosion blast injury in China: 2000-2017 data from the State Administration of Work Safety

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Military Medical Research 2021年第1期8卷 1-8页

作者： Xu Wang Juan Du Zhuo Zhuang Zheng-Guo Wang jian-xin jiang Ce Yang State Key Laboratory of Trauma Burn and Combined InjuryResearch Institute of SurgeryDaping HospitalArmy Medical UniversityChongqing 400042China Department of Emergency the Second Affiliated Hospital of Medical College in Zhejiang UniversityHangzhou 310009China Applied Mechanics Laboratory School of AerospaceTsinghua UniversityBeijing 100084China

Background: Civilian explosion blast injury is more frequent in developing countries, including China. However, the incidence, casualties, and characteristics of such incidents in China are ***: This is a retrospective analysis of the State Administration of Work Safety database. Incidents during a period from January 1, 2000 to April 30, 2017 were included in the analysis. The explosions were classified based on the number of deaths into extraordinarily major, major, serious and ordinary type. Descriptive statistics was used to analyze the incidence and characteristics of the explosions. Correlation analysis was performed to examine the potential correlations among various ***: Data base search identified a total of 2098 explosions from 2000 to 2017, with 29,579 casualties: 15,788 deaths(53.4%), 12,637 injured(42.7%) and 1154 missing(3.9%). Majority of the explosions were serious type(65.4%). The number of deaths(39.5%) was also highest with the serious type(P=0.006). The highest incidence was observed in the fourth quarter of the year(October to December), and at 9:00–11:00 am and 4:00–6:00 pm of the day. The explosions were most frequent in coal-producing provinces(Guizhou and Shanxi Province). Coal mine gas explosions resulted majority of the deaths(9620, 60.9%). The number of explosion accidents closely correlated with economic output(regional economy and national GDP growth rate)(r=–0.372, P=0.040;r=0.629, P=0.028).Conclusions: The incidence and civilian casualties due to explosions remain unacceptable in developing China. Measures that mitigate the risk factors are of urgently required.

关键词： Explosion Blast exposure Blast mitigation Overpressure Trauma and injury

The Effects of Solid Phase Additives on Sintering Properties of Alumina Bioceramic

在线全文

学校读者我要写书评

暂无评论

Journal of Wuhan University of Technology(Materials Science) 2003年第2期18卷 23-24页

作者：王欣宇 Wuhan University of Technology

In order to reduce the sintering temperature and improve the preparing conditions of alumina bioceramics,the Mg-Zr-Y composite solid phase additives were added into high purity Al 2O 3 micro-powder by chemical coprecipitation *** powder was shaped under 200MPa cold isostatic pressure,and then the biscuits were sintered at 1600℃ under normal *** sintered alumina materials were tested and the sintering mechanism was *** results show that physical properties of the material were improved *** Mg-Zr-Y composite solid additives could promote the sintering of alumina bioceramics and the mechanism is solid phase sintering.

关键词： alumina sintering additives property

Transesterified Chinese Spicehush (Lindera communis) seed oil as a biodiesel fuel

在线全文

学校读者我要写书评

暂无评论

Forestry Studies in China 2007年第2期9卷 132-136页

作者： Wang Wei-gang Ma Lei jiang jian-xin jiang Li Zhang Zhi-xiang College of Materials Science and Technology Beijing Forestry University Beijing 100083 P. R. China

Studies were carried out on the transesterification of the Chinese Spicehush （Lindera communis） seed （LCS） oil with methanol for the production of biodiesel. The methyl esters of LCS oil were compared with soybean esters to determine biodiesel fuel performance and properties. The fatty acids content in the seed kernel oil （LKO） and coat oil （LCO） were quantified by gas chromatography and mass spectrometry method （C-C-MS）. LKO contains 95.1% saturated acids （capric, lauric, myristic and palmitic） and 2.3% unsaturated acids （oleic）. LCO contains 24.6% saturated acids （palmitic and stearic） and 73.6% unsaturated acids （oleic, linoleic and palmitoleic）. The kinematic viscosity, cold filter plugging point, cloud point, flash point and cetane index were determined. The methyl esters of LKO had a higher kinematic viscosity and a lower cold filter plugging point value, so it has better performance in cold weather. The biodiesel of LCS oil has fuel properties within the limits prescribed by American （ASTM D 6751-02） standards, except for a slightly lower flash point of LKO biodiesel than that prescribed by these standards （130℃）. Thus, LKO and LCO biodiesel have great potential to be used on a large scale as fuel for diesel engines.

关键词： biodiesel Lindera communis oil transesterification property

Induction of acute hepatic injury by endotoxin in mice

在线全文

学校读者我要写书评

暂无评论

Hepatobiliary & Pancreatic Diseases International 2002年第4期1卷 558-564页

作者： Guo-Qi Xie jian-xin jiang Yong-Hua Chen Da-Wei Liu Pei-Fang Zhu Zheng-Guo Wang Emergency Department 152nd Hospital PLA Pingdingshan 467000 China.

Objective: To investigate the changes of scavenger re-ceptor （SR） and CD14 in Kupffer cells in endotox-emia in order to uncover the mechanism of the liverto turn a defense organ into effector one in ***: Mouse models of endotoxemia of differentseverity were reproduced by injection of different do-ses of lipopolysaccharide （LPS） via the tail *** expression of SR and CD14 in the liver was as-sayed by immunohistochemistry and was subsequent-ly analyzed with an image analysis system. The levelsof TNF-α and IL-6 in liver tissue were determinedwith ***: The expression of SR in the liver in the high-dose group was markedly decreased one hour afterinjection of LPS, and also in the low-dose group at 3hours. The expression of SR in the liver in the twogroups was shown to be progressively decreased withthe time prolonged. There was significant differencein average optical density （OD） values of SR be-tween the two groups. The expression of CD14 in thetwo groups was shown to be significantly increasedone hour after injection of LPS, and more signifi-cantly with the time prolonged. But there was no sig-nificant difference in OD values of CD14 between thetwo groups. The contents of intrahepatic proinflam-matory mediators TNF-α, IL-6, ALT and TBILwere significantly increased after injection of *** analysis revealed that the changes ofTNF-α, IL-6, ALT, and TBIL were negatively cor-related with the expression of SR, and positively withthe expression of CD14.Conclusion: The up-regnlation of CD14 expressionand down-regulation of SR expression on Kupffercells might be one of the important mechanisms forthe conversion of Kupffer cells from immune defen-sive to inflammatory response cells in acute hepaticinjury.

关键词： hepatic injury endotoxemia scavenger receptor CD14 tumor necrosis factor-α interleukin

Nanomaterials: Applications in the diagnosis and treatment of pancreatic cancer

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Pharmacology and Therapeutics 2020年第1期11卷 1-7页

作者： Jie Wang Zhi-Wei He jian-xin jiang Department of Hepatic-Biliary Surgery Renmin Hospital of Wuhan UniversityWuhan 430060Hubei ProvinceChina

Pancreatic cancer(PC)remains one of the leading causes of cancer-related death in human sowing to missed early and effective *** inability to translate research into clinical trials and to target chemotherapy drugs to tumors is a major obstacle in PC *** with traditional cancer detection methods,the method combining existing clinical diagnosis and detection systems with nanoscale components using novel nanomaterials shows higher sensitivity and *** can interact with biological systems to efficiently and accurately detect and monitor biological events during diagnosis and *** the advance of experimental and engineering technology,more nanomaterials will begin the transition to clinical trials for their *** paper describes a number of nanomaterials used in the diagnosis and treatment of PC.

关键词： Nanomaterials Pancreatic cancer Diagnosis Treatment Nanoparticles Quantum dots

Continuous purification and culture of rat type 1 and type 2 alveolar epithelial cells by magnetic cell sorting

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Traumatology 2022年第3期25卷 138-144页

作者： Di Liu jian-Hui Sun Hua-Cai Zhang jian-xin jiang Ling Zeng State Key Laboratory of Trauma Bums and Combined InjuriesDoping HospitalArmy Medical UniversityChongqing400042China Medical Center of Trauma and War Injury Daping HospitalArmy Medical UniversityChongqing400042China

Purpose:The incidence of acute lung injury(ALI)in severe trauma patients is 48%and the mortality rate following acute respiratory distress syndrome evolved from ALI is up to 68.5%.Alveolar epithelial type 1 cells(AEC1 s)and type 2 cells(AEC2s)are the key cells in the repair of injured lungs as well as fetal lung ***,the purification and culture of AECls and AEC2s play an important role in the research of repair and regeneration of lung ***:Sprague-Dawley rats(3-4 weeks,120-150 g)were purchased for *** and DNase I were jointly used to digest lung tissue to obtain a single-cell suspension of whole lung cells,and then magnetic bead cell sorting was performed to isolate Tla positive cells as AECls from the single-cell suspension by using polyclonal rabbit anti-Tla(a specific AECls membrane protein)antibodies combined with anti-rabbit IgG ***,alveolar epithelial cell membrane marker protein EpCAM was designed as a key label to sort AEC2s from the remaining Tla-neg cells by another positive immunomagnetic selection using monoclonal mouse anti-EpCAM antibodies and anti-mouse IgG *** purity was identified by immunofluorescence staining and flow ***沾••The purity of AECls and AEC2s was 88.3%±3.8%and 92.6%±2.7%,*** cell growth was observed as follows:AECls stretched within the 12-16 h,but the cells proliferated slowly;while AEC2s began to stretch after 24 h and proliferated rapidly from the 2nd day and began to differentiate after 3 ***:AECls and AEC2s sorted by this method have high purity and good ***,our method provides a new approach for the isolation and culture of AECls and AEC2s as well as a new strategy for the research of lung repair and regeneration.

关键词： Alveolar epithelial cells type 1 Alveolar epithelial cells type 2 Three-dimensional culture Magnetic activated cell sorting

Sepsis-induced immunosuppression:mechanisms,diagnosis and current treatment options

在线全文

学校读者我要写书评

暂无评论

Military Medical Research 2023年第2期10卷 242-261页

作者： Di Liu Si-Yuan Huang jian-Hui Sun Hua-Cai Zhang Qing-Li Cai Chu Gao Li Li Ju Cao Fang Xu Yong Zhou Cha-Xiang Guan Sheng-Wei Jin Jin Deng Xiang-Ming Fang jian-xin jiang Ling Zeng Department of Trauma Medical Center Daping HospitalState Key Laboratory of TraumaBurns and Combined InjuryArmy Medical UniversityChongqing 400042China Department of Respiratory Disease Daping HospitalArmy Medical UniversityChongqing 400042China Department of Laboratory Medicine the First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Department of Critical Care Medicine the First Affiliated Hospital of Chongqing Medical University400016 ChongqingChina Department of Physiology School of Basic Medicine ScienceCentral South UniversityChangsha 410078China Department of Anesthesia and Critical Care the Second Affiliated Hospital and Yuying Children’s Hospital of Wenzhou Medical UniversityZhejiang 325027 WenzhouChina Department of Emergency the Affiliated Hospital of Guizhou Medical UniversityGuizhou Medical University550001 GuiyangChina Department of Anesthesiology the First Affiliated HospitalZhejiang University School of MedicineHangzhou 310052China.

Sepsis is a common complication of combat injuries and trauma,and is defined as a life-threatening organ dysfunction caused by a dysregulated host response to *** is also one of the significant causes of death and increased health care costs in modern intensive care *** use of antibiotics,fluid resuscitation,and organ support therapy have limited prognostic impact in patients with *** its pathophysiology remains elusive,immunosuppression is now recognized as one of the major causes of septic ***-induced immunosuppression is resulted from disruption of immune *** is characterized by the release of antiinflammatory cytokines,abnormal death of immune effector cells,hyperproliferation of immune suppressor cells,and expression of immune *** targeting immunosuppression,especially with immune checkpoint inhibitors,preclinical studies have demonstrated the reversal of immunocyte dysfunctions and established host ***,we comprehensively discuss recent findings on the mechanisms,regulation and biomarkers of sepsis-induced immunosuppression and highlight their implications for developing effective strategies to treat patients with septic shock.

关键词： Sepsis Immunosuppression Immune monitoring Immunomodulatory therapy

Residual Levels and New Inputs of Chlorinated POPs in Agricultural Soils from Taihu Lake Region

在线全文

学校读者我要写书评

暂无评论

Pedosphere 2005年第3期15卷 301-309页

作者： GAOHong-jian jiangxin WANGFang BIANYong-Rong WANGDai-Zhang DENDjian-Cai YANDong-Yun InstituteofSoilScience ChineseAcademyofSciencesNanjing210008（China）

Selected persistent organochlorine pesticides (OCPs), including 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) and its principal metabolites 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene (DDE) and 1,1-dichloro-2,2-bis(p-chlorophenyl)e- thane (DDD), hexachlorocyclohexane (HCH) and its isomers (α-,β-, γ-, and δ-HCH), hexachlorobenzene (HCB), endo- sulfan, dieldrin, and endrin were quantified to determine current levels of organochlorine pesticides, to assess the eco- toxicological potential, and to distin…

关键词： agricultural soils chlorinated persistent organic pollutants (POPs) new input residual levels Taihu Lake region