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Reversible and Irreversible Drought-Induced Changes in the Anther Proteome of Rice （Oryza sativa L.） Genotypes IR64 and Moroberekan

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Molecular Plant 2011年第1期4卷 59-69页

作者： jian-xiang liu John Bennett Plant Breeding Genetics and Biochemistry Division International Rice Research Institute Metro Manila Philippines State Key Laboratory of Genetic Engineering Institute of Plant Biology School of Life Sciences Fudan University Shanghai 200433 China

Crop yield is most sensitive to water deficit during the reproductive stage. For rice, the most sensitive yield component is spikelet fertility and the most sensitive stage is immediately before heading. Here, we examined the effect of drought on the anther proteome of two rice genotypes： Moroberekan and IR64. Water was withheld for 3 d before head- ing （3DBH） in well watered controls for 5 d until ＂the flag leaf relative water content （RWC） had declined to 45-50%. Plants were then re-watered and heading occurred 2-3 d later, representing a delay of 4-5 d relative to controls. The anther proteins were separated at 3 DBH, at the end of the stress period, and at heading in stressed/re-watered plants and con- trols by two-dimensional （2-D） gel electrophoresis, and 93 protein spots were affected reproducibly in abundance by drought during the experiment across two rice genotypes. After drought stress, upon re-watering, expressions of 24 pro- tein spots were irreversible in both genotypes, 60 protein spots were irreversible in IR64 but reversible in Moroberekan, only nine protein spots were irreversible in Moroberekan while reversible in IR64. Among them, there were 14 newly drought-induced protein spots in IR64; none of them was reversible on re-watering. However, there were 13 newly drought-induced protein spots in Moroberekan, 10 of them were reversible on re-watering, including six drought-induced protein spots that were not reversed in IR64. Taken together, our proteomics data reveal that drought-tolerant genotype Moroberekan possessed better recovery capability following drought and re-watering at the anther proteome level than the drought-sensitive genotype IR64. The disruptions of drought to rice anther development and pollen cell functions are also discussed in the paper.

关键词： Rice (Oriza sativa L.) anther development drought proteomics

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Chromatin remodeling factors regulate environmental stress responses in plants

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Journal of Integrative Plant Biology 2021年第3期63卷 438-450页

作者： Ze-Ting Song jian-xiang liu Jia-Jia Han Yunnan Key Laboratory of Plant Reproductive Adaptation and Evolutionary Ecology Yunnan UniversityKunming 650500China State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China Laboratory of Ecology and Evolutionary Biology State Key Laboratory for Conservation and Utilization of Bio‐Resources in YunnanYunnan UniversityKunming 650500China

Environmental stress from climate change and agricultural activity threatens global plant biodiversity as well as crop yield and *** sessile organisms,plants must maintain the integrity of their genomes and adjust gene expression to adapt to various environmental *** eukaryotes,nucleosomes are the basic unit of chromatin around which genomic DNA is packaged by *** enable dynamic access to packaged DNA,eukaryotes have evolved Snf2(sucrose nonfermenting 2)family proteins as chromatin remodeling factors(CHRs)that modulate the position of nucleosomes on *** plant stress responses,CHRs are recruited to specific genomic loci,where they regulate the distribution or composition of nucleosomes,which in turn alters the accessibility of these loci to general transcription or DNA damage repair ***,CHRs interplay with other epigenetic mechanisms,including DNA methylation,histone modifications,and deposition of histone *** are also involved in RNA processing at the posttranscriptional *** this review,we discuss major advances in our understanding of the mechanisms by which CHRs function during plants’response to environmental stress.

关键词： chromatin remodeling environmental stress epigenetic regulation replication stress stress responses

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Mutation of DELAYED GREENING1 impairs chloroplast RNA editing at elevated ambient temperature in Arabidopsis

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Journal of Genetics and Genomics 2020年第4期47卷 201-212页

作者： Jingliang Sun Yingying Tian Qichao Lian jian-xiang liu State Key Laboratory of Genetic Engineering School of Life SciencesFudan UniversityShanghai200433China State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou310027China

Chloroplasts are important for plant growth and *** editing in chloroplast converts cytidines(Cs)to uridine s(Us)at specific transcript positions and provides a correction mechanism to restore conserved codons or creates start or stop ***,the underlined molecular mechanism is not yet fully unders *** the present study,we identi fied a thermo-sensi tive mutantin leaf color 1(tst1)and found that TSL1 is allelic to DELAYED GREENING 1(DG1).The mis sense mutation of DG1 in tsl1 mutant confers a high temperature sensitivity and impaired chloroplast development at an elevated ambient temperature in *** analysis showed that chloroplast RNA editing at seve ral sites including accD-2568,ndhD-2,and petL-5 is impaired in tsl1 mutant plants grown at an elevated ***1 interacts with MORF2 and other proteins such as DYW1 and DYW2 involved in chloroplast RNA *** vitro RNA electrophoretic mobility shift assay demonstrated that DG1 binds to RNA targets such as accD,ndhD,and ***,our results revealed that DG1 is important for maintaining chloroplast mRNA editing in Arabidopsis.

关键词： Chloroplast development mRNA editing Warm temperature

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NFXL1 functions as a transcriptional activator required for thermotolerance at reproductive stage in Arabidopsis

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Journal of Integrative Plant Biology 2024年第1期66卷 54-65页

作者： Qiao‐Yun Zhu Lin‐Lin Zhang jian‐xiang liu State Key Laboratory of Plant Environmental Resilience College of Life SciencesZhejiang UniversityHangzhou 310027China

Plants are highly susceptible to abiotic stresses,particularly heat stress during the reproductive ***,the specific molecular mechanisms underlying this sensitivity remain largely *** the current study,we demonstrate that the Nuclear Transcription Factor,X-box Binding Protein 1-Like 1(NFXL1),directly regulates the expression of DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 2A(DREB2A),which is crucial for reproductive thermotolerance in ***1 is upregulated by heat stress,and its mutation leads to a reduction in silique length(seed number)under heat stress ***-Seq analysis reveals that NFXL1 has a global impact on the expression of heat stress responsive genes,including DREB2A,Heat Shock Factor A3(HSFA3)and Heat Shock Protein 17.6(HSP17.6)in flower ***,NFXL1 is enriched in the promoter region of DREB2A,but not of either HSFA3 or *** experiments using electrophoretic mobility shift assay have confirmed that NFXL1 directly binds to the DNA fragment derived from the DREB2A ***,effector-reporter assays have shown that NFXL1 activates the DREB2A *** DREB2A mutants are also heat stress sensitive at the reproductive stage,and DEREB2A is epistatic to NFXL1 in regulating thermotolerance in flower *** is known that HSFA3,a direct target of DREB2A,regulates the expression of heat shock proteins genes under heat stress ***,our findings establish NFXL1 as a critical upstream regulator of DREB2A in the transcriptional cassette responsible for heat stress responses required for reproductive thermotolerance in Arabidopsis.

关键词： Arabidopsis DREB2A heat stress NFXL1 reproductive thermotolerance

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CCaP1/CCaP2/CCaP3 interact with plasma membrane H^(+)-ATPases and promote thermoresponsive growth by regulating cell wall modification in Arabidopsis

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Plant Communications 2024年第7期5卷 90-105页

作者： Jing-Jing Wang Juan Gao Wei Li jian-xiang liu State Key Laboratory of Plant Environmental Resilience College of Life SciencesZhejiang UniversityHangzhou 310027China College of Environmental and Resource Sciences Zhejiang UniversityHangzhou 310027China

Arabidopsis plants adapt to warm temperatures by promoting hypocotyl growth primarily through the basic helix-loop-helix transcription factor PIF4 and its downstream genes involved in auxin responses,which enhance cell *** the current study,we discovered that cell wall-related calcium-binding protein 2(CCaP2)and its paralogs CCaP1 and CCaP3 function as positive regulators of thermo-responsive hypocotyl growth by promoting cell elongation in ***,mutations in CCaP1/CCaP2/CCaP3 do not affect the expression of PIF4-regulated classic downstream ***,they do noticeably reduce the expression of xyloglucan endotransglucosylase/hydrolase genes,which are involved in cell wall *** also found that CCaP1/CCaP2/CCaP3 are predominantly localized to the plasma membrane,where they interact with the plasma membrane H^(+)-ATPases AHA1/***,we observed that vanadate-sensitive H^(+)-ATPase activity and cell wall pectin and hemicellulose contents are significantly increased in wild-type plants grown at warm temperatures compared with those grown at normal growth temperatures,but these changes are not evident in the ccap1-1 ccap2-1 ccap3-1 triple ***,our findings demonstrate that CCaP1/CCaP2/CCaP3 play an important role in controlling thermo-responsive hypocotyl growth and provide new insights into the alternative pathway regulating hypocotyl growth at warm temperatures through cell wall modification mediated by CCaP1/CCaP2/CCaP3.

关键词： AHA1/AHA2 CCaP1/CCaP2/CCaP3 cell wall modification hypocotyl growth thermomorphogenesis warm temperature

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Rescuing the Golgi from heat damages by ATG8:restoration rather than clean-up

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Stress Biology 2023年第1期3卷 219-221页

作者： Anni Luo jian-xiang liu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China

High temperature stress poses significant adverse effects on crop yield and *** the molecular mechanisms underlying heat stress tolerance in plants/crops,especially regarding the organellar remodeling and homeostasis,are largely *** a recent study,Zhou et *** that autophagy-related 8(ATG8),a famous regulator involved in autophagy,plays a new role in Golgi restoration upon heat *** apparatus is vacuolated following short-term acute heat stress,and ATG8 is translocated to the dilated Golgi membrane and interacts with CLATHRIN LIGHT CHAIN 2(CLC2)to facilitate Golgi restoration,which is dependent on the ATG conjugation system,but not of the upstream autophagic *** exciting findings broaden the fundamental role of ATG8,and elucidate the organelle-level restoration mechanism of Golgi upon heat stress in plants.

关键词： ATG8 Autophagy CLC2 ER Golgi restoration Heat stress

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Real-world evidence on the efficacy and safety of vonoprazanamoxicillin dual therapy for Helicobacter pylori treatment in elderly patients

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World Journal of Gastroenterology 2025年第1期31卷 68-78页

作者： Wen Gao Jing-Wen Li Hui Ye Xue-Zhi Zhang jian-xiang liu Hong Cheng Department of Gastroenterology Peking University First HospitalBeijing 100034China School of Medicine Tsinghua MedicineTsinghua UniversityBeijing 100034China Department of Traditional Chinese Medicine and Integrative Medicine Peking University First HospitalBeijing 100034China

BACKGROUND A dual therapy regimen containing amoxicillin is a common treatment option for the eradication of Helicobacter pylori(***).While substantial research supports the efficacy and safety of vonoprazan and amoxicillin(VA)dual therapy in the general population,there is still a lack of studies specifically focusing on its safety in elderly *** To evaluate efficacy and safety of VA dual therapy as first-line or rescue treatment for *** in elderly *** As a real-world retrospective study,data were collected from elderly patients aged 60 years and above who accepted VA dual therapy(vonoprazan 20 mg twice daily+amoxicillin 1000 mg thrice daily for 14 days)for *** eradication in the Department of Gastroenterology at Peking University First Hospital between June 2020 and January *** status was evaluated by^(13)C-urease breath test 6 weeks after *** adverse events(AEs)during treatment were *** In total,401 cases were ***-one cases were excluded due to loss to follow-up,lack of re-examination,or unwillingness to take *** total of 380 included cases comprised 250 who received VA dual therapy as first-line treatment and 130 who received VA dual therapy as rescue *** was successfully eradicated in 239 cases(95.6%)in the first-line treatment group and 116 cases(89.2%)in the rescue treatment *** overall incidence of AEs was 9.5%for both ***,9.2%of patients experienced an AE in the first-line treatment group and 10.0%in the rescue treatment *** patients discontinued treatment due to AE,with a discontinuation rate of 1.3%.No serious AE *** The VA dual therapy regimen as a first-line treatment and a rescue therapy was effective and safe for elderly patients aged 60 and older.

关键词： Helicobacter pylori Vonoprazan Amoxicillin Dual therapy Elderly

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TT3.1:a journey to protect chloroplasts upon heat stress

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Stress Biology 2022年第1期2卷 358-360页

作者： Jin‑Yu Li jian‑xiang liu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China

Rice(Oryza sativa L.)is a staple crop that feeds over half the world’s *** temperature stress is a great threaten to sustainable agriculture and leads to yield loss and impaired grain quality in major *** is sensitive to heat stress at almost all the growth stages and the molecular mechanisms underlying responses to heat stress in rice is *** quantitative trait locus(QTL)mapping,a recent study conducted by Zhang et *** that one genetic locus Thermo-tolerance 3(TT3)contains two genes that are required for thermotolerance in *** TT3.1–TT3.2 genetic module in rice links the plasma membrane to chloroplasts to protect chloroplasts from heat stress damage and increases grain yield under heat stress *** breakthrough provides a promis-ing strategy for future breeding of high temperature resilient crops.

关键词： Chloroplast ER Heat stress NTL3 Plasma membrane TT3.1 TT3.2

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bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis

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aBIOTECH 2022年第1期3卷 1-11页

作者： Juan Gao Mei-ing Wang Jing-Jing Wang Hai-Ping Lu jian-xiang liu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China

High temperature elicits a well-conserved response called the unfol ded protein response(UPR)to bring protein homeostasis in the endoplasmic reticulum(ER).Two key UPR regulators bZIP28 and bZIP60 have been shown to be essential for maintaining fertility under heat stress conditions in Arabidopsis,however,the function of transcriptional activator bZIP17,a paralog of bZIP28,in heat stress response at reproductive stage is not *** we found that bzip17 mutant plants were sensitive to heat stress in terms of silique length and fertility comparing to that of wildtype(WT)Arabidopsis plants,and transcri ptomic analysis showed that 1380 genes were specifically up-regulated and 493 genes were specifically down-regulated by heat stress in the flowers of WT plants comparing to that in bzip17 mutant *** bZIP17-dependent up-regulated genes were enriched in responses to a biotic stresses such as water deprivation and salt *** chromatin immuno-preci pitation coupled with high-throughput sequencing(ChIP-Seq)uncovered 1645 genes that were direct targets of bZIP17 in MYC-bZIP17 expressing seedlings subjected to heat *** these 1645 genes,ERSE-II cis-element was enriched in the binding peaks of their promoters,and the up-regulation of 113 genes by heat stress in flowers was dependent on *** results revealed direct targets of bZIP17 in flowers during heat stress responses and demonstrated the important role of bZIP17 in maintai ning fertility upon heat stress in plants.

关键词： Arabidopsis bZIP17 Flower Fertility Heat Stress UPR

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The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis

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Journal of Integrative Plant Biology 2021年第6期63卷 1097-1103页

作者： Lin-Lin Zhang Wei Li Ying-Ying Tian Seth Jon Davis jian-xiang liu State Key Laboratory of Plant Physiology and Biochemistry College of Life SciencesZhejiang UniversityHangzhou 310027China Department of Biology University of YorkHeslingtonYork YO105DDUK State Key Laboratory of Crop Stress Biology School of Life SciencesHenan UniversityKaifeng 475004China

Plants are capable of coordination of their growth and development with ambient *** FLOWERING3(ELF3), an essential component of the plant circadian clock, is also involved in ambient temperature sensing, as well as in inhibiting the expression and protein activity of the thermoresponsive regulator phytochrome interacting factor4(PIF4). The ELF3 activity is subjected to attenuation in response to warm temperature;however,how the protein level of ELF3 is regulated at warm temperature remains less understood. Here, we report that the E3 ligase XB3 ORTHOLOG 5 IN ARABIDOPSIS THALIANA, XBAT35, mediates ELF3 degradation. XBAT35 interacts with ELF3 and ubiquitinates ELF3. Loss-of-function mutation of XBAT35 increases the protein level of ELF3 and confers a short-hypocotyl phenotype under warm temperature conditions. Thus, our findings establish that XBAT35 mediates ELF3 degradation to lift the inhibition of ELF3 on PIF4 for promoting thermoresponsive hypocotyl growth in plants.

关键词： The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis ELF

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