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Chinese Chemical Letters 2006年第6期17卷 757-760页

作者： jian wen jiang Ming Zhong CAI Department of Chemistry Jiangxi Normal University Nanchang 330027

（E）-α-Iodovinyl sulfides 1 underwent the Sonogashira coupling reactions with terminal alkynes 2 in piperidine at room temperature in the presence of 5 mol % of Pd（PPh3）4 and 10 mol % of CuI to afford the correspo... 详细信息

关键词： (E)-α-Iodovinyl sulfide 1 3-enynylsulfide Sonogashira coupling terminal alkyne.

A facile stereospecific synthesis of (Z)-2-sulfonyl-substituted 1,3-enynes via Sonogashira coupling of (E)-α-iodovinyl sulfones with 1-alkynes

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Chinese Chemical Letters 2007年第7期18卷 773-776页

作者： wen Yan Hao jian wen jiang Ming Zhong Cai Department of Chemistry Jiangxi Normal University Nanchang 330022 China

（E）-α-Iodovinyl sulfones 1 underwent the Sonogashira coupling reactions with terminal alkynes 2 in piperidine at room temperature in the presence of 5 mol% of Pd（PPh3）4 and 10 mol% of CuI to stereospecifically af... 详细信息

关键词： (E)-α-Iodovinyl sulfone 1,3-Enynylsulfone Sonogashira coupling Terminal alkyne

Gut microbial dysbiosis associates hepatocellular carcinoma via the gut-liver axis

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Hepatobiliary & Pancreatic Diseases International 2019年第1期18卷 19-27页

作者： jian-wen jiang Xin-Hua Chen Zhigang Ren Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health First Affiliated Hospital Zhejiang University School of Medicine Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University Health Management Center First Affiliated Hospital Zhejiang University School of Medicine Department of Infectious Disease Precision Medicine Center the First Affiliated Hospital of Zhengzhou University

Background: Hepatocellular carcinoma(HCC) is one of the most common malignancies in the world. Gut microbiota has been demonstrated to play a critical role in liver inflammation, chronic fibrosis, liver cirrhosis, and HCC development through the gut-liver axis. Data sources: Recently there have been several innovative studies investigating gut microbial dysbiosismediated enhancement of HCC through the gut-liver axis. Literatures from January 1998 to January 2018 were searched in the Pub Med database using the keywords "gut microbiota" and "hepatocellular carcinoma" or "liver cancer", and the results of experimental and clinical studies were analyzed. Results: Gut microbial dysbiosis accompanies the progression of alcoholic liver disease, non-alcoholic fatty liver disease and liver cirrhosis, and promotes HCC progression in an experimental mouse model. The immune system and key factors such as Toll-like receptor 4 are involved in the process. There is evidence for gut microbial dysbiosis in hepatitis virus-related HCC patients. Conclusions: Gut microbial dysbiosis is closely associated with hepatic inflammation disease and HCC through the gut-liver axis. With the enhanced understanding of the interactions between gut microbiota and liver through the gut-liver axis, new treatment strategies for HCC are being developed.

关键词： Gut-liver axis Gut microbiota Hepatocellular carcinoma Lipopolysaccharide Toll-like receptor 4

Effect of chip rate on the ranging accuracy in a regenerative pseudo-noise ranging system

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Journal of Zhejiang University-Science C(Computers and Electronics) 2011年第2期12卷 132-139页

作者： jian-wen jiang Wei-jun YANG Chao-jie ZHANG Xiao-jun JIN Zhong-he JIN Department of Information Science and Electronic Engineering Zhejiang University Hangzhou 310027 China

The ranging accuracy of a pseudo-noise ranging system is mainly decided by range jitter and time delay discrimination. Many factors can affect the ranging accuracy, one of which is the chip rate. In digital signal processing, the time delay discrimination and autocorrelation function of sampled ranging sequences of different chip rates are very different. An approximation simulation model is established according to an in-phase quadrature (I/Q) correlator which is used to evaluate the time delay. Simulation results of the range jitter and time delay discrimination show that the chip rate which provides a non-integer sample-to-chip rate ratio can achieve a higher ranging accuracy, and some test results validate the simulation model. In some design missions, the simulation results may help to select an optimum sample-to-chip rate ratio to satisfy the design requirement on the ranging accuracy.

关键词： Ranging accuracy Range jitter Time delay discrimination Sample-to-chip rate ratio Integration time Autocorrelation function Fractional chip period delay

Application of metagenomics in the human gut microbiome

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World Journal of Gastroenterology 2015年第3期21卷 803-814页

作者： Wei-Lin Wang Shao-Yan Xu Zhi-Gang Ren Liang Tao jian-wen jiang Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery First Affiliated HospitalZhejiang University School of Medicine

There are more than 1000 microbial species living in the complex human *** gut microbial community plays an important role in protecting the host against pathogenic microbes,modulating immunity,regulating metabolic processes,and is even regarded as an endocrine ***,traditional culture methods are very limited for identifying *** the application of molecular biologic technology in the field of the intestinal microbiome,especially metagenomic sequencing of the next-generation sequencing technology,progress has been made in the study of the human intestinal *** can be used to study intestinal microbiome diversity and dysbiosis,as well as its relationship to health and ***,functional metagenomics can identify novel functional genes,microbial pathways,antibiotic resistance genes,functional dysbiosis of the intestinal microbiome,and determine interactions and co-evolution between microbiota and host,though there are still some ***,metaproteomics and metabolomics represent enormous complements to the understanding of the human gut *** review aims to demonstrate that metagenomics can be a powerful tool in studying the human gut microbiome with encouraging *** limitations of metagenomics to be overcome are also ***,metaproteomics and metabolomics in relation to the study of the human gut microbiome are also briefly discussed.

关键词： Human gut microbiome Metabolomics Metagenomics Met

Ginsenoside Rg_3 inhibit hepatocellular carcinoma growth via intrinsic apoptotic pathway

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World Journal of Gastroenterology 2011年第31期17卷 3605-3613页

作者： jian-wen jiang Xin-Mei Chen Xin-Hua Chen Shu-Sen Zheng The Key Lab of Combined Multi-organ Transplantation Ministry of Public HealthThe Department of Hepatobiliary and Pancreatic SurgeryFirst Affiliated HospitalSchool of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvinceChina The Department of Pharmacy Shandong Traditional Chinese Medicine UniversityJinan 250355Shandong ProvinceChina

AIM:To investigate the anti-tumor function of ginsenoside Rg3 on hepatocellular carcinoma(HCC) in vitro and in vivo,and its ***:Hep1-6 and HepG2 cells were treated by Rg3 in different concentrations(0,50,100 and 200 μg/mL) in *** incubation for 0,6,12,24 and 48 h,cell viability was measured by 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyltetrazolium bromide *** was identified by terminal deoxynucleotidyl transferasemediated dUTP-biotin nick end ***-3 activity was measured by chromophore p-nitroanilide and flow ***-2 family proteins were ascertained by *** membrane potentialwas detected by 5,5',6' 6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine *** liver tumor-bearing C57Bl6 mice were divided randomly into 4 groups for intra-tumor injection of saline,ginsenoside Rg3,cyclophosphamide(CTX) and ginsenoside Rg3 + CTX ***:The survival time was followed up to 102 *** mice in the Rg3 + CTX group showed significant increased survival time compared with those in the control group(P < 0.05).Rg3 could inhibit HCC cell proliferation and induce cell apoptosis in vitro in the concentration and time dependent *** also induced mitochondria membrane potential to ***-3 activation can be blocked by the inhibitor *** was up-regulated while Bcl-2 and Bcl-XL were down-regulated after Rg3 ***:Our data suggested that Rg3 alone or combined with CTX inhibited tumor growth in vivo and prolonged mouse survival time by inducing HCC cell apoptosis via intrinsic pathway by expression alterations of Bcl-2 family proteins.

关键词： Ginsenoside Rg3 Apoptosis Hepatocellular Carcinoma Bcl-2 family proteins Cyclophosphamide

Optimal immunosuppressor induces stable gut microbiota after liver transplantation

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World Journal of Gastroenterology 2018年第34期24卷 3871-3883页

作者： jian-wen jiang Zhi-Gang Ren Hai-Feng Lu Hua Zhang Ang Li Guang-Ying Cui Jun-Jun Jia Hai-Yang Xie Xin-Hua Chen Yong He Li jiang Lan-Juan Li Department of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital School of Medicine Zhejiang University Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases Zhejiang University Health Management Center First Affiliated Hospital School of Medicine Zhejiang University Department of Infectious Diseases Precision Medicine Center the First Affiliated Hospital of Zhengzhou University State Key Laboratory for Diagnosis and Treatment of Infectious Disease First Affiliated Hospital School of Medicine Zhejiang University

AIM To study the influence of different doses of tacrolimus(FK506)on gut microbiota after liver transplantation(LT)in *** Specific pathogen-free Brown Norway(BN)rats and Lewis rats were separated into five groups:(1)Tolerance group(BN-BN LT,n=8);(2)rejection group(Lewis-BN LT,n=8);(3)high dosage FK506(FK506-H)group(Lewis-BN LT,n=8);(4)middle dosage FK506(FK506-M)group(Lewis-BN LT,n=8);and(5)low dosage FK506(FK506-L)group(LewisBN LT,n=8).FK506 was administered to recipients at a dose of 1.0 mg/kg,0.5 mg/kg,and 0.1 mg/kg body weight for 29 d after LT to the FK506-H,FK506-M,and FK506-L groups,*** the 30^(th) day after LT,all rats were sampled and *** samples were harvested for liver function and plasma endotoxin *** graft and ileocecal tissues were collected for histopathology *** contents were used for DNA extraction,Real-time quantitative polymerase chain reaction(RT-PCR)and digital processing of denaturing gradient gel electrophoresis(DGGE)profiles and *** Compared to the FK506-H and FK506-L groups,FK506-M was optimal for maintaining immunosuppression and inducing normal graft function;the FK506-M maintained gut barrier integrity and low plasma endotoxin levels;furthermore,DGGE results showed that FK506-M induced stable gut *** analysis indicated that FK506-M increased species richness and rare species abundance,and cluster analysis confirmed the stable gut microbiota induced by *** tree analysis identified crucial bacteria associated with FK506-M;seven of the nine bacteria that were decreased corresponded to Bacteroidetes,while increased bacteria were of the Bifidobacterium ***506-M increased Faecalibacterium prausnitzii and Bifidobacterium *** decreased Bacteroides-Prevotella and Enterobacteriaceae,as assessed by RT-PCR,which confirmed the crucial bacterial alterations identified through *** Compared to the low or high dosage of FK5

关键词： Liver transplantation Graft function Gut microbiota Immunosuppressor Tacrolimus Rejection Denaturing gradient gel electrophoresis

Relation of overexpression of S phase kinase-associated protein 2 with reduced expression of p27 and PTEN in human gastric carcinoma

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World Journal of Gastroenterology 2005年第42期11卷 6716-6721页

作者： Xiu-Mei Ma Ying Liu jian-wen Guo jiang-Hui Liu Lian-Fu Zuo The Fourth Affiliated Hospital of Hebei Medical University Shijiazhuang 050011 Hebei Province China

AIM： TO investigate the significance of S phase kinase- associated protein 2 （Skp2） expression in human gastric carcinoma and the relation between expressions of Skp2, p27 and PTEN. METHODS： Immunohistochemical analysis was performed on 138 gastric carcinoma specimens, their paired adjacent mucosa specimens, 102 paired lymphatic metastatic carcinoma tissue specimens, 30 dysplasia specimens, 30 intestinal metaplasia specimens, 10 chronic superficial gastritis specimens and 5 normal gastric mucosa specimens for Skp2 expression and on 138 gastric carcinoma specimens for p27 and PTEN expression. RESULTS： Skp2 labeling frequency was significantly higher in intestinal rnetaplasia （12.68±0.86） and adjacent rnucosa （19.324±1.22） than in normal gastric rnucosa （0.53±0.13） and chronic superficial gastritis （0.47±0.19）（P = 0.000）, in dysplasia （16.74±0.82）than in intestinal metaplasia （P = 0.000）; in gastric primary carcinoma （31.34±2.17） than in dysplasia and adjacent rnucosa （P = 0.000）, in metastasis gastric carcinoma in lymph nodes （39.76±2.00） than in primary gastric carcinoma （P = 0.037）, respectively. Skp2 labeling frequency was positively associated with differentiation degree （rho = 0.315, P = 0.000）, vessel invasion （rho = 0.303, P = 0.000） and lymph node metastasis （rho = 0.254, P = 0.000） of gastric cancer. Expression of Skp2 was negatively associated with p27 （rho = -0.451, P = 0.000） and PTEN （rho = -0.480, P = 0.000） expression in gastric carcinoma, p27 expression was positively associated with PTEN expression in gastric carcinoma （rho = 0.642, P = 0.000）.CONCLUSION： Skp2 overexpression may be involvedin carcinogenesis and progression of human gastric carcinoma in vivo, possibly via p27 proteolysis. PTEN may regulate the expression of p27 by negatively regulating Skp2 expression.

关键词： Gastric carcinoma Skp2 p27 PTEN

Chronic bile duct hyperplasia is a chronic graft dysfunction following liver transplantation

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World Journal of Gastroenterology 2012年第10期18卷 1038-1047页

作者： jian-wen jiang Zhi-Gang Ren Guang-Ying Cui Zhao Zhang Hai-Yang Xie Lin Zhou The Key Lab of Combined Multi-organ Transplantation Ministry of Public Health The Department of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 Zhejiang Province China State Key Laboratory for Diagnosis and Treatmerit of Infectious Disease First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 Zhejiang Province China

AIM: To investigate pathological types and influential factors of chronic graft dysfunction (CGD) following liver transplantation (LT) in rats. METHODS: The whole experiment was divided into three groups: (1) Normal group (n = 12): normal BN rats without any drug or operation; (2) SGT group (syngeneic transplant of BN-BN, n = 12): both donors and recipients were BN rats; and (3) AGT group (allogeneic transplant of LEW-BN, n = 12): Donors were Lewis and recipients were BN rats. In the AGT group, all recipients were subcutaneously injected by Cyclosporin A after LT. Survival time was observed for 1 year. All the dying rats were sampled, biliary tract tissues were performed bacterial culture and liver tissues for histological study. Twenty-one d after LT, 8 rats were selected randomly in each group for sampling. Blood samples from caudal veins were collected for measurements of plasma endotoxin, cytokines and metabonomic analysis, and faeces were analyzed for intestinal microflora. RESULTS: During the surgery of LT, no complications of blood vessels or bile duct happened, and all rats in each group were still alive in the next 2 wk. The long term observation revealed that a total of 8 rats in the SGT and AGT groups died of hepatic graft diseases, 5 rats in which died of chronic bile duct hyperplasia. Compared to the SGT and normal groups, survival ratio of rats significantly decreased in the AGT group (aP < 0.01, bP < 0.001, respectively). Moreover, liver necrosis, liver infection, and severe chronic bile duct hyperplasia were observed in the AGT group by H and E stain. On 21 d after LT, compared with the normal group (25.38 ± 7.09 ng/L) and SGT group (33.12 ± 10.26 ng/L), plasma endotoxin in the AGT group was remarkably increased (142.86 ± 30.85 ng/L) (both P < 0.01). Plasma tumor necrosis factor-α and interleukin-6 were also significantly elevated in the AGT group (593.6 ± 171.67 pg/mL, 323.8 ± 68.30 pg/mL) vs the normal (225.5 ± 72.07 pg/mL, 114.6 ± 36.67 pg/mL) and

关键词： Liver transplantation Chronic graft dysfunction Chronic bile duct hyperplasia Metabonomics Intestinal barrier function

Enteral supplementation with glycyl-glutamine improves intestinal barrier function after liver transplantation in rats

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Hepatobiliary & Pancreatic Diseases International 2011年第4期10卷 380-385页

作者： jian-wen jiang,Zhi-Gang Ren,Lu-Yan Chen,Li jiang,Hai-Yang Xie,Lin Zhou and Shu-Sen Zheng Key Laboratory of Combined Multi-organ Transplantation,Ministry of Public Health Department of Hepatobiliary and Pancreatic Surgery,First Affiliated Hospital,Zhejiang University School of Medicine,Hangzhou 310003,China Key Laboratory of Combined Multi-organ Transplantation Ministry of Public Health Department of Hepatobiliary and Pancreatic Surgery First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China

BACKGROUND:Most patients after liver transplantation (LT) suffer from intestinal barrier ***-glutamine (Gly-Gln) by parenteral supplementation is hydrolyzed to release glutamine,which improves intestinal barrier function in intestinal *** study aimed to investigate the effect of GlyGln by enteral supplementation on intestinal barrier function in rats after allogenetic LT under immunosuppressive ***:Twelve inbred Lewis rats were selected randomly as donors,and 24 inbred Brown Norway (BN) rats as recipients of allogenetic *** recipients were divided into a control group (Ala,n=12) and an experimental group (Gly-Gln,n=12).In each group,6 normal BN rats were sampled for normal parameters on preoperative day *** 6 recipients in the control group received alanine (Ala) daily by gastric perfusion for 3 preoperative days and 7 postoperative days,and the 6 recipients in the experimental group were given Gly-Gln in the same *** 12 BN recipients underwent orthotopic LT under sterile conditions after a 3-day fast and were given immunosuppressive therapy for 7 *** were harvested for sampling on postoperative day *** following parameters were assessed:intestinal mucosal protein content,mucosal ultrastructure,ileocecal sIgA content,portal plasma levels of endotoxin and TNF-α,and bacterial ***:All recipients were alive after *** preoperative day 3,all parameters were similar in the two *** postoperative day 8,all parameters in the two groups were remarkably changed from those on preoperative day ***,compared to the Ala group,supplementation withGly-Gln increased the levels of intestinal mucosal protein and ileocecal sIgA,improved mucosal microvilli,and decreased portal plasma levels of endotoxin and TNF-α as well as bacterial ***:Enteral supplementation with Gly-Gln improved intestinal barrier function after allogenetic LT in rats.

关键词： liver transplantation glycyl-glutamine bacterial translocation intestinal barrier function