检索结果-南通市图书馆

Preliminary Study on a Potential Panel for Quality Assurance of ELISPOT

Virologica Sinica 2008年第5期23卷 330-338页

作者： Chun-tao ZHANG Yu WU Chen-yan ZHAO Kun-xue HONG Chun-yu LIU Ying WANG Ping ZHONG jian-hui nie Xue-lin WU You-chun WANG Department of Cell Biology National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China Division of Virology and Immunology National Center for AIDS Control and Prevention Beijing 100050 China Shanghai Center for Disease Control and Prevention Shanghai 200336 China

The ELISPOT assay is increasingly used for assessing cellular immune responses in clinical trials of HIV-1 or cancer vaccines. However, to date, data from clinical trials do not consistently show that immune responses are correlated with clinical endpoints. This is due in part to the lack of assay standardization and validation across laboratories and therefore, a quality control panel is required to establish competency and comparability amongst different laboratories. In this study peripheral blood mononuclear cells (PBMCs) from healthy individuals were screened and frozen in liquid nitrogen. The recovery and viability of the PBMCs and the frequencies of interferon (IFN)-γ-secreting cells after CEF peptide pool stimulation were detected after various intervals in seven different laboratories. The recovery and viability did not differ significantly after different intervals. Although the frequencies of IFN (interferon)-γ-secreting cells among thawed PBMCs (peripheral blood mononuclear cells) fluctuated after CEF peptide pool stimulation at different intervals, they were not significantly decreased compared with those among fresh PBMCs. However, the viabilities, recoveries and frequencies of IFN-γ-secreting cells differed significantly among the seven laboratories. Our results indicate that cryopreserved PBMCs could be used as a quality control panel for ELISPOT. However, the procedures for ELISPOT need to be standardized amongst different laboratories.

关键词： ELISPOT Peripheral blood mononuclear cells (PBMCs) Cryopreservation Interferon-γ CEFpeptide pools

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Ideal Bi‑Based Hybrid Anode Material for Ultrafast Charging of Sodium‑Ion Batteries at Extremely Low Temperatures

引用

Nano-Micro Letters 2025年第3期17卷 152-167页

作者： Jie Bai jian hui Jia Yu Wang Chun Cheng Yang Qing jiang Key Laboratory of Automobile Materials(Jilin University) Ministry of EducationSchool of Materials Science and EngineeringJilin UniversityChangchun 130022People’s Republic of China

Sodium-ion batteries have emerged as competitive substitutes for low-temperature applications due to severe capacity loss and safety concerns of lithium-ion batteries at−20°C or ***,the key capability of ultrafast charging at ultralow temperature for SIBs is rarely ***,a hybrid of Bi nanoparticles embedded in carbon nanorods is demonstrated as an ideal material to address this issue,which is synthesized via a high temperature shock *** a hybrid shows an unprecedented rate performance(237.9 mAh g^(−1) at 2 A g^(−1))at−60℃,outperforming all reported SIB anode *** with a Na_(3)V_(2)(PO_(4))_(3)cathode,the energy density of the full cell can reach to 181.9 Wh kg^(−1) at−40°*** on this work,a novel strategy of high-rate activation is proposed to enhance performances of Bi-based materials in cryogenic conditions by creating new active sites for interfacial reaction under large current.

关键词： Bi nanoparticles High temperature shock High-rate activation Ultrafast charging Low-temperature sodium-ion batteries

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Comparison of the Immunogenicities of HIV-1 Mutants Based on Structural Modification of env

引用

Virologica Sinica 2008年第4期23卷 233-246页

作者： jian-hui nie Chun-tao ZHANG hui-hui CHONG Xue-ling WU Chun-yu LIU Yu WU Chen-yan ZHAO Lin-qi ZHANG You-chun WANG Department of Cell Biology National Institute for the Control of Pharmaceutical and Biological Products No 2 Tiantanxili Beijing 100050 China College of Life Science Jilin University Changchun 130012 China Aaron Diamond AIDS Research Center The Rockefeller University 455 First Avenue New York NY 10016 USA

Eleven env mutants were designed and generated by site-directed mutagenesis of the regions around NAb epitopes and deletions of variable regions in env. The immunogenicities of the generated mutants were evaluated using single-cycle infection neutralization assays with two pseudoviruses and IFN-γ ELISPOT. Overall, five mutants (dWt, M2, M5-2, M5-1 and dM7) induced higher neutralization activities for both pseudoviruses than plasmid Wt, while only two of the mutants (dWt and M5-2) showed significant differences (P<0.05). Two mutants (M2 and dM2) induced more Env-specific T cells than plasmid Wt. Statistically however, significance was only reached for mutant M2. Thus, properly modified HIV-1 Env may have the potential to induce potent cellular and humoral immune responses.

关键词： HIV-1 Env Modification Neutralization assay ELISPOT

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Positive effect of Mo on mechanical properties of a Ni-42W-10Co-xMo medium heavy alloy

引用

China Foundry 2025年第1期22卷 108-116页

作者： Guo-xin Cao jian-xin Dong Yong-sheng He He jiang Fang Hao Zhi-hua nie Teng-fei Ma Bao-quan Fu School of Materials Science and Engineering University of Science and Technology BeijingBeijing 100083China Xi'an Superalloy Technology Co. Ltd.Xi’an 710299China School of Materials Science and Engineering Beijing Institute of TechnologyBeijing 100081China Key Laboratory of Air-driven Equipment Technology of Zhejiang Province Quzhou UniversityQuzhou 324000ZhejiangChina

In this study,a novel Ni-W-Co-Mo medium heavy alloy(MHA)was designed to improve its mechanical strength via Mo *** the Ni-42W-10Co-x Mo alloy series,where x represents the weight percent of Mo and varies between 0,1,2,5,and 10,the microstructure transitions from a dendritic structure to a hypo-eutectic structure as the Mo content increases from 0 to 5wt.%.Moreover,as the Mo content increases from 0 to 10wt.%,the distribution of theμ-phase shifts from being individually dispersed to forming aggregates,and its volume fraction rises from 0.5%to 7.9%.Notably,theμ-phase evolves into an eutectic microstructure,which helps in minimizing the segregation of *** change is accompanied by a substantial enhancement in mechanical properties;specifically,the compressive yield strength at room temperature increases from 350 MPa to 646 MPa,indicating a significant 85%***,the microhardness increases from 230 HV to 304 *** dynamics simulations further reveal that the strengthening mechanism of Ni-42W-10Co-x Mo alloys is Mo-induced solid solution strengthening and precipitation strengthening.

关键词： medium-heavy alloys Ni-based alloy mechanical properties solid solution strengthening precipitation strengthening

来源：

维普期刊数据库

同方期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effect of lanthanum on microstructure of a nickel-based single crystal superalloy

引用

China Foundry 2025年第1期22卷 55-64页

作者： Hong Gao Kai Guan Ren-jie Cui jian-chao Qin Zi-han Zhao Zhao-hui Huang Science and Technology on Advanced High Temperature Structural Materials Laboratory Beijing Institute of Aeronautical MaterialsBeijing 100095China

To enhance the high-temperature oxidation resistance and mechanical properties of a secondgeneration nickel-based superalloy,various concentrations of lanthanum(La)ranging from 5.0×10^(-5)wt.%to 3.4×10^(-4)wt.%are added to the *** microstructure of the nickel-based single crystal superalloy with trace of La was examined by means of SEM,EDS and *** show the addition of La decreases the segregation of elements and increases the amount ofγ/γ′eutectics of the as-cast alloy,and in the interdendritic region,the growth of eutectics is accompanied by the growth of strip clusters composed of Ni_(5)La and Ni_(3)*** the La content in the alloy increases,the proportion of Ni_(5)La in the cluster *** heat treatment,incipient melting occurs in the cluster regions,leading to an increase in microporosity compared to the original as-cast ***,the heat treatment alters the shape of the clusters from a strip morphology to an elliptical one,and it changes their composition from Ni_(5)La and Ni_(3)Ta to a combination of Ni_(5)La,Ni_(3)Ta,and MC carbides.

关键词： single crystal superalloy lanthanum microstructure heat-treatment precipitate phase

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The interaction between KIF21A and KANK1 regulates dendritic morphology and synapse plasticity in neurons

引用

Neural Regeneration Research 2025年第1期20卷 209-223页

作者： Shi-Yan Sun Lingyun nie Jing Zhang Xue Fang Hongmei Luo Chuanhai Fu Zhiyi Wei Ai-hui Tang Hefei National Research Center for Physical Sciences at the Microscale CAS Key Laboratory of Brain Function and DiseaseMinistry of Education Key Laboratory for Membrane-less Organelles and Cellular DynamicsDivision of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Institute of Artificial Intelligence Hefei Comprehensive National Science CenterHefeiAnhui ProvinceChina CAS Center for Excellence in Molecular Cell Sciences Division of Life Sciences and MedicineUniversity of Science and Technology of ChinaHefeiAnhui ProvinceChina Department of Neurobiology School of Life SciencesSouthern University of Science and TechnologyShenzhenGuangdong ProvinceChina Brain Research Center Southern University of Science and TechnologyShenzhenGuangdong ProvinceChina

Morphological alterations in dendritic spines have been linked to changes in functional communication between neurons that affect learning and ***-4 KIF21A helps organize the microtubule-actin network at the cell cortex by interacting with KANK1;however,whether KIF21A modulates dendritic structure and function in neurons remains *** this study,we found that KIF21A was distributed in a subset of dendritic spines,and that these KIF21A-positive spines were larger and more structurally plastic than KIF21A-negative ***,the interaction between KIF21A and KANK1 was found to be critical for dendritic spine morphogenesis and synaptic *** of either KIF21A or KANK1 inhibited dendritic spine morphogenesis and dendritic branching,and these deficits were fully rescued by coexpressing full-length KIF21A or KANK1,but not by proteins with mutations disrupting direct binding between KIF21A and KANK1 or binding between KANK1 and *** down KIF21A in the hippocampus of rats inhibited the amplitudes of long-term potentiation induced by high-frequency stimulation and negatively impacted the animals’cognitive *** together,our findings demonstrate the function of KIF21A in modulating spine morphology and provide insight into its role in synaptic function.

关键词： actin cytoskeleton dendrite KANK1 KIF21A microtubule spine morphology spine synaptic plasticity talin1

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Landscape Stability Assessment and Simulation Analysis Under Urban Expansion:A Case Study of Hangzhou, China

引用

Chinese Geographical Science 2025年第02期 311-325页

作者： PEI hui ZHANG Lin ZHOU Minli nie Wenbin ZHOU Shihao SHI Yan PAN jianyun College of Landscape Architecture Zhejiang Agriculture and Forestry University Huzhou Spatial Planning Compilation and Research Center Anji County Huzhou City Township Planning and Management Station Institute of Ecological Civilization & Institute of Carbon Neutrality Zhejiang A&F University

In recent years, rapid urbanization has had a profound impact on landscape stability. As a typical example of China's rapid urbanization, Hangzhou has also experienced significant landscape changes, which have profoundly affected its ecological *** Hangzhou as an example, this study integrates land use change data from 1980 to 2020, combines dynamic simulation and ecological modeling techniques, and carries out a comprehensive analysis of historical trends and future predictions, to provide valuable insights into the complex interactions between urban expansion and landscape stability. The results indicate that: 1) between 1980 and2020, Hangzhou experienced a significant increase in construction land at the expense of arable land, leading to a gradual decline in landscape stability, though the downward trend has slowed in recent years. 2) The spatial distribution of landscape stability shows clear aggregation patterns, with lower stability concentrated in economically active flatlands and higher stability in the mountainous western regions. 3) By 2040, further urban expansion is predicted to occur alongside increased landscape integration, reflecting the positive effects of ecological protection strategies. This study highlights the universal challenges of balancing economic growth with ecological stability in rapidly urbanizing regions. The combination of advanced simulation models and spatiotemporal analysis demonstrates a replicable framework for assessing urban expansion's ecological impacts. These findings underscore the importance of tailoring urban planning and ecological policies to address regional disparities, providing valuable insights for sustainable urban development and landscape management globally.

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

A novel method for simultaneous measurement of^(222)Rn and^(220)Rn progeny concentrations measured by an alpha spectrometer

引用

Nuclear Science and Techniques 2025年第1期36卷 127-139页

作者： Zhong-Kai Fan Jia-Le Sun Hao-Xuan Li Xiang-Ming Cai hui Yang Shou-Kang Qiu Yan-Liang Tan jian Shan School of Nuclear Science and Technology University of South ChinaHengyang 421001China College of Physics and Electronic Engineering Hengyang Normal UniversityHengyang 421008China

The accumulation of^(222)Rn and^(220)Rn progeny in poorly ventilated environments poses the risk of natural radiation exposure to the public.A previous study indicated that satisfactory results in determining the^(222)Rn and^(220)Rn progeny concentrations by measuring the total alpha counts at five time intervals within 560 min should be expected only in the case of high progeny concentrations in *** complete the measurement within a relatively short period and adapt it for simultaneous measurements at comparatively lower^(222)Rn and^(220)Rn progeny concentrations,a novel mathematical model was proposed based on the radioactive decay *** model employs a nonlinear fitting method to distinguish nuclides with overlapping spectra by utilizing the alpha particle counts of non-overlapping spectra within consecutive measurement cycles to obtain the concentrations of^(222)Rn and^(220)Rn progeny in *** verification experiments were conducted using an alpha *** experimental results demonstrate that the concentrations of^(222)Rn and^(220)Rn progeny calculated by the new method align more closely with the actual circumstances than those calculated by the total count method,and their relative uncertainties are all within±16%.Furthermore,the measurement time was reduced to 90 min,representing an acceleration of 84%.The improved capability of the new method in distinguishing alpha particles with similar energies emitted from ^(218)Po and^(212)Bi,both approximately 6 MeV,contributed to realizing more accurate *** proposed method has the potential advantage of measuring relatively low concentrations of^(222)Rn and^(220)Rn progeny in air more quickly via air filtration.

关键词： ^(222)Rn ^(220)Rn Progeny concentration Nonlinear fitting method Alpha spectrometer

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Accelerating homogenization kinetics and enhancing hot-workability of as-cast nickel-based superalloy via prior hot-deformation

引用

Journal of Iron and Steel Research International 2025年第1期32卷 215-226页

作者： Jia-jun Chen Yang Zhou Hao-ran Han Xian-guang Zhang Goro Miyamoto Ping-mei Tang Dong-ping Xiao jian-hui Fu Peng Shi Yi-wu Pei jian Zhang State Key Laboratory of Advanced Metallurgy University of Science and Technology BeijingBeijing 100083China School of Metallurgical and Ecological Engineering University of Science and Technology BeijingBeijing 100083China Chengdu Advanced Metal Materials Industrial Technology Institute Co. Ltd.Chengdu 610300SichuanChina Institute for Materials Research Tohoku UniversitySendai 980-8577Aoba-KuJapan

Enhancing homogenization efficiency and hot-workability is the key issue for wrought superalloys in the industry.A novel approach for simultaneous accelerating the homogenization kinetics and improving hot-workability via a simple way of prior hot-deformation was proposed,which was not widely accepted for wrought *** homogenization efficiency is increased by 40%-70%via performing 10%-20%prior *** theoretical and experimental analyses revealed that the increment in homogenization efficiency is mainly attributed to the decrease in interdendritic-segregation spacing,and thus the necessary diffusion distance,rather than that of *** addition,dynamic and static recrystallizations occurred during the prior hot-deformation and diffusion-annealing processes,and the grains were significantly refined even after the ***,the size of the precipitates was refined as *** enhanced the hot-workability of the homogenized ingot for the subsequent cogging process.

关键词： Nickel-based superalloy Homogenization kinetics Prior hot-deformation Grain size Hot workability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Prediction of microstructure evolution of ZK61 alloy during hot spinning by internal state variable model

引用

中国有色金属学报 2025年第1期35卷 126-142页

作者： Jin-qi PAN Wen-cong ZHANG jian-lei YANG Song-hui WANG Yong WU Huan LI School of Materials Science and Engineering Harbin Institute of Technology WeihaiWeihai 264209China College of Mechanical and Electrical Engineering Nanjing University of Aeronautics and AstronauticsNanjing 210016China

An internal state variable(ISV)model was established according to the experimental results of hot plane strain compression(PSC)to predict the microstructure evolution during hot spinning of ZK61 *** effects of the internal variables were considered in this ISV model,and the parameters were optimized by genetic *** validation,the ISV model was used to simulate the evolution of grain size(GS)and dynamic recrystallization(DRX)fraction during hot spinning via Abaqus and its subroutine *** comparing the simulated results with the experimental results,the application of the ISV model was proven to be ***,the strength of the thin-walled spun ZK61 tube increased from 303 to 334 MPa due to grain refinement by DRX and texture ***,some ultrafine grains(0.5μm)that played an important role in mechanical properties were formed due to the proliferation,movement,and entanglement of dislocations during the spinning process.

关键词： internal state variable model hot spinning ZK61 alloy finite element simulation texture evolution

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：