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High temperature corrosion behavior of arc-sprayed aluminum coatings on steel

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中国有色金属学会会刊：英文版 2004年第Z1期14卷 451-454页

作者： SUN jian-bo wang yong DONG Li-xian College of Mechanical and Electronic Engineering University of Petroleum Dongying 257061 China College of Mechanical and Electronic Engineering University of Petroleum Dongying 257061 China College of Mechanical and Electronic Engineering University of Petroleum Dongying 257061 China

Aluminum coatings were sprayed on the substrate of steel Q235 by arc spraying. The test samples of Q235, Al coatings and Al coatings with seal coat were placed in box type electric resistance furnace at 400, 500,600, 650 ℃ when their oxidation behaviors were studied. And their oxidation kinetics curves were protracted. Microstructure, microhardness, bond strength and distribution of section elements were investigated by optical microscope(OM), Vickers microhardness instrument, electric tensile test machine and EPMA. Al coatings and Al coatings with seal coat can enhance the oxidation-resistance of substrate under 500 ℃. The latter has the best corrosion resistance. The coatings can't protect the substrate against oxidation above 600 ℃. After a long time corrosion there is enriched oxygen element at interface.

关键词： arc spraying Al coating Al coating with seal coat high temperature corrosion oxidation kineticscurve

The Critical Group of Km × Cn

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Acta Mathematica Sinica,English Series 2011年第1期27卷 169-184页

作者： jian wang yong Liang PAN Jun Ming XU Department of Mathematics University of Science and Technology of China Hefei 230026 P. R. China

In this paper, the structure of the critical group of the graph Km × Cn is determined, where m, n ≥3.

关键词： Graph Laplacian matrix critical group invariant factor Smith normal form tree number

Calcineurin signalling mechanisms in myocardial hypertrophy

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Journal of Geriatric Cardiology 2010年第3期7卷 189-192页

作者： jian-Chun wang yong Zhao jian-Hua Shao Department of Geriatric cardiology Shandong Provincial Hospital Jinan 250021 China

Calcineurin dephosphorylates multiple serine residues near the N terminus of NFAT proteins enabling them to translocate from cytoplasm to nucleus, where they activate a subset of hypertrophic response genes. Transgenic mice over-expressing a constitu- tively active form of calcineurin or NFAT3, developed obviously hypertrophy and heart failure or sudden death proving its pathogenic role. Here we used literatures on MEDLINE （2000-2011）, systematically reviewed the new development of calcineurin signaling pathway in myocardial hypertrophy

关键词： Calcineurin signal transduction hypertrophy

Epigallocatechin-3-gallate attenuates lipopolysaccharideinduced inflammation in human retinal endothelial cells

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International Journal of Ophthalmology(English edition) 2014年第3期7卷 408-412页

作者： Hui-Yan Zhang jian-yong wang Hang-Ping Yao Hangzhou Vocational & Technical College Department of Ophthalmology the First Affiliated HospitalSchool of Medicine Zhejiang University State Key Laboratory for Diagnosis and Treatment of Infectious Diseases the First Affiliated Hospital School of Medicine Zhejiang University

AIM:To investigate the mechanism underlying the anti-inflammatory effects of epigallocatechin-3-gallate(EGCG)in lipopolysaccharide(LPS)-stimulated human retinal endothelial cells(HRECs).METHODS:HRECspre-treatedwithEGCG(0-100μmol/L)were stimulated with LPS(250 ng/mL).Levels of tumor necrosis factor alpha(TNF-α),vascular endothelial growth factor(VEGF),monocyte chemotactic protein-1(MCP-1)and nitric oxide(NO)in the supernatants were determined by enzyme-linked immunosorbent assay(ELISA)and Griess *** protein expression of phosphorylated extracellular signal-regulated kinase(ERK)1/2 and p38 mitogen-activated protein kinases(p38)were determined by Western blot ***:EGCG pre-treatment significantly inhibited the secretion of TNF-α,VEGF,MCP-1 and NO in LPSstimulated ***,EGCG effectively attenuated LPS-induced activation and phosphorylation of ERK1/2 and p38 in HRECs in a dose-dependent ***:EGCG exhibited inhibitory effects on LPS-induced pro-inflammatory cytokines production by modulating ERK1/2 and p38 pathways in HRECs,suggesting EGCG as a potential candidate for antiinflammatory intervention.

关键词： epigallocatechin-3-gallate human retinal endothelial cells inflammatory factors

MGB probe assay for rapid detection of mtDNA11778 mutation in the Chinese LHON patients by real-time PCR

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2008年第8期9卷 610-615页

作者： jian-yong wang Yang-shun GU Jing wang Yi TONG Ying wang Jun-bing SHAO Ming QI Department of Ophthalmology the First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 310003 China Department of Ophthalmology the First Affiliated Hospital Fujian Medical University Fuzhou 325000 China Department of Ophthalmology Eye Hospital Academy of Chinese Medical Science Beijing 100040 China Institute of Biotechnology Shanghai Zhijiang Biotech Incorporation Shanghai 210038 China Institute of Genome Sciences University of Rochester Medical Center Rochester NY 14642 USA

Objective： Leber＇s hereditary optic neuropathy （LHON） is a maternally inherited degeneration of the optic nerve caused by point mutations of mitochondrial DNA （mtDNA）. Many unsolved questions regarding the penetrance and pathophysiological mechanism of LHON demand efficient and reliable mutation testing. This study aims to develop a minor groove binder （MGB） probe assay for rapid detection of mtDNA11778 mutation and heteroplasmy in Chinese LHON patients by real-time polymerase chain reaction （PCR）. Methods： Forty-eight patients suspected of having LHON and their maternal relatives underwent a molecular genetic evaluation, with 20 normal individuals as a control group at the same time. A real-time PCR involving two MGB probes was used to detect the mtDNA 1 1778 mutation and heteroplasmy. A linear standard curve was obtained by pUCmLHONG and pUCmLHONA clones. Results： All 48 LHON patients and their maternal relatives were positive for rntDNA11778 mutation in our assay, 27 heteroplasmic and 21 homoplasmic. Eighteen cases did not show an occurrence of the disease, while 9 developed the disease among the 27 heteroplasmic mutation cases. Eleven did not show an occurrence of the disease, while 10 cases developed the disease among 21 homoplasmic mutation cases. There was a significant difference in the incidence between the heteroplasmic and the homoplasmic mutation types. The time needed for running a real-time PCR assay was only 80 min. Conclusion： This real-time PCR assay is a rapid, reliable method for mtDNA mutation detection as well as heteroplasmy quantification. Detecting this ratio is very important for predicting phenotypic expression of unaffected carriers.

关键词： Leber's hereditary optic neuropathy (L HON) Mitochondrial DNA (mtDNA) MtDNA 11778 mutation Minor groove binder (MGB) orobe. Real-time oolvmerase chain reaction (PCR)

Immobilized chitosan as a selective absorbent for the nickel removal in water sample

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Journal of Environmental Sciences 2003年第5期15卷 633-638页

作者： WU jian min, wang yong yao (Department of Chemistry, College of Science, Zhejiang University, Hangzhou 310027, China. ) Department of Chemistry College of Science Zhejiang University Hangzhou 310029

Method for preparation of chitosan immobilized on silica gel(CTS silica) was described. The CTS silica was used as absorbent for the absorption of nickel in water. The results showed that this absorbent had relatively high selectivity and strong affinity to nickel. The maximum absorption capacity for nickel can reach 667 mg/g of chitosan. Factors that affect the absorption capacity, such as pH, ion strength and the presence of calcium, EDTA and the mechanism of absorption were discussed in detail. The absorbent can be regenerated with acid and reused for several times. The recovery rate for nickel can reach 99 99%. This absorbent filled in a column can be used in nickel removal from wastewater and drinking water.

关键词： immobilized chitosan nickel wastewater treatment

Solidification Properties of CaO-SiO_2-TiO_2 Based Mold Fluxes

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Journal of Iron and Steel Research International 2011年第7期18卷 15-19,52页

作者： HE Sheng-ping HUANG Qing-yun ZHANG Guo-xing LU yong-jian wang Qian Chongqing Key Laboratory of Metallurgical Engineering College of Materials Science and EngineeringChongqing University Chongqing 400044 China

In continuous casting of peritectic steel grades sensitive to longitudinal cracking, the solidification properties of mold fluxes play an important role in keeping smooth running of continuous casting process and achieving high surface quality of casting strands. To reduce fluorine pollution in molten slag, types of CaO-SiO2-TiO2 （CTS） based mold fluxes were investigated. The solidification and crystallization properties, including viscosity η at 1 573 K, break temperature Tbr, crystallization ratio Rc and solidification mineragraphy were measured, which were compared with those of CaO-SiO2-CaF2 based mold fluxes. The experiments show that there are unstable viscosity-high tem- perature properties and high Tbr in part of CTS slag system, which are bad for lubrication between liquid flux film and strand. And when temperature is below Tbr, the viscosities change slowly during cooling in some part of this slag system, which imply that liquid mold fluxes solidify slowly and it is easy to cause surface longitudinal cracks on strand. Major mineragraphy of the CaO-SiO2-TiO2 based mold fluxes are CaO · TiO2 or CaO · TiO2 and CaO · SiO2 · TiO2. TiN and Ti（C,N） can be formed in mold fluxes bearing high TiO2 during the continuous casting.

关键词： mold flux solidification property peritectic steel mineragraphy

Serum lipids and other risk factors for diabetic retinopathy in Chinese type 2 diabetic patients

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第5期14卷 392-399页

作者： Hui-yan ZHANG jian-yong wang Gui-shuang YING Li-ping SHEN Zhe ZHANG Hangzhou Vocational & Technical College Department of Ophthalmology the First Affiliated HospitalSchool of MedicineZhejiang University Center for Preventive Ophthalmology and Biostatistics Department of OphthalmologyPerelman School of MedicineUniversity of Pennsylvania Department of Endocrinology the First Affiliated HospitalSchool of MedicineZhejiang University

Objective: To investigate the association of serum lipids and other risk factors with diabetic retinopathy (DR) in Chinese type 2 diabetic patients. Methods: Five hundred and twenty-three type 2 diabetic patients underwent ophthalmic examination by experienced retinal specialists to assess their DR. Serum lipids, including triglycerides, total cholesterol, high density lipoprotein cholesterol (HDLC), and low density lipoprotein cholesterol (LDLC), were measured using Roche automated clinical chemistry analyzers. The concentration of very low density lipoprotein cholesterol (VLDLC) was calculated based on total cholesterol, HDLC and LDLC. Hyperlipidemia was defined as a total cholesterol concentration of 6.2 mmol/L or higher or the use of lipid-lowering medications. The association of risk factors with any DR or proliferative diabetic retinopathy (PDR) was assessed using the odds ratio (OR) and its 95% confidence interval (CI), calculated from logistic regression models. Results: In multivariate logistic regression models, hyperlipidemia (OR=2.39, 95% CI: 1.02-5.66), higher VLDLC (OR=1.59, 95% CI: 1.14-2.23), and higher triglyceride (OR=1.18, 95% CI: 1.03-1.37) were associated with increased risk of DR. A longer diabetic duration was associated with increased risk of DR (P<0.0001) and PDR (P=0.002) in a dose-response manner. Higher systolic blood pressure (P=0.02) and higher serum creatinine (P=0.01) were independently associated with increased risk of DR, and female gender was associated with increased risk of PDR (P=0.03). Conclusions: Among Chinese type 2 diabetic patients, hyperlipidemia, higher VLDLC, and higher triglyceride were independently associated with increased risk of DR, suggesting control of serum lipids may decrease the risk of DR.

关键词： Diabetic retinopathy Serum lipids Risk factors

Ramet Population Structure of Fargesia nitida （Mitford） Keng f. et Yi in Different Successional Stands of the Subalpine Coniferous Forest in Wolong Nature Reserve

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Journal of Integrative Plant Biology 2006年第10期48卷 1147-1153页

作者： Xiao-Hong Yu jian-Ping Tao Yuan Li yong-jian wang Yi Xi Wei-Yin Zhang Run-Guo Zang Key Laboratory of Eco-environments of Three Gorges Reservoir Region Ministry of Education School of Life Sciences Southwest University Chongqing 400715 China Institute of Forest Ecology Environment and Protection Chinese Academy of Forestry Beijing 100091 China

Forest structure and succession in Wolong Nature Reserve is influenced by the understory dwarf bamboo population. However, less is known about how the forest succession affects the dwarf bamboo population. To examine the bamboo ramet population growth of Fargesla nitida （Mltford） Keng f. et Yi and to determine how ramet population structure varies along the succession of coniferous forest, we sampled ramet populations of F. nitida from the following three successional stages：（i） a deciduous broad-leaved （BL） stand; （ii） a mixed broad-leaved coniferous （MI） stand; and （ill） a coniferous （CF） stand. We investigated the population structure, biomass allocation, and morphological characteristics of the bamboo ramet among the three stand types. Clonal ramets, constituting the bamboo population, tended to become short and small with succession. The ramet changed towards having a greater mass investment in leaves, branches and underground roots and rhizomes rather than in the culm. With respect to leaf traits, individual leaf mass and area in the BL stand were markedly bigger than those In both the MI and CF stands, except for no significant difference in specific leaf area. The age distribution showed that the bamboo population approached an older age with succession. The results demonstrate that the ramet population structure of F. nitida is unstable and its growth performance is inhibited by succession.

关键词： coniferous forest Fargesia nitida ramet population structure succession Wolong Nature Reserve