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Biological impacts of "hot-spot" mutations of hepatitis B virus X proteins are genotype B and C differentiated

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World Journal of Gastroenterology 2005年第30期11卷 4703-4708页

作者： Xu lin Xiao Xu Qing-ling Huang Yu-Qing Liu Da-Li Zheng Wan-Nan Chen jian-yin lin Research Center of Molecular Medicine Fujian Medical University Fuzhou 350004 Fujian Province China Centre for the Study of Liver Disease and Department of Surgery The University of Hong Kong Pokfulam Hong Kong China

AIM: To investigate the biological impacts of 'hot-spot'mutations on genotype B and C HBV X proteins (HBx).METHODS: Five types of'hot-spot' mutations of genotype B or C HBV X genes, which sequentially lead to the amino acid substitutions of HBx as I127T, F132Y, K130M+V131I,I127T+K130M+V131I, or K130M+V131I+F132Y, respectively,were generated by means of site-directed *** evaluate the anti-proliferative effects, HBx or related mutants' expression vectors were transfected separately to the Chang cells by lipofectamine, and the cells were cultured in hygromycin selective medium for 14 d, drug-resistant colonies were fixed with cold methanol, stained with Giemsa dyes and scored (increase of the colonies indicated the reduction of the anti-proliferation activity,and vice versa). Different types of HBx expression vectors were co-transfected separately with the reporter plasmid pCMVβ to Chang cells, which were lysed 48 h post-transfection and the intra-cellular β-galactosidase activities were monitored (increase of the β-galactosidase activities indicated the reduction of the transactivation activity, and vice versa). All data obtained were calculated by paired-samples ***: As compared to standard genotype B HBx,mutants of I127T and I127T+K130M+V131I showed higher transactivation and anti-proliferative activities, while the mutants of F132Y, K130M+V131I, and K130M+V131I+F132Y showed lower activities. As compared to standard genotype C HBx, I127T mutant showed higher transactivation activity, while the other four types of mutants showed no differences. With regard to anti-proliferative activity,compared to standard genotype C HBx, F132Y and K130M+V131I mutants showed lower activities, and K130M+V131I +F132Y mutant, on the other hand, showed higher activity,while the mutants of I127T and I127T+K130M+V131I showed no ***: 'Hot-spot' mutations affect the antiproliferation and transactivation activities of genotype B and/or C HBx, and the

关键词： Hepatitis B virus Genotype X gene Mutation

Influence of enterococci on human sperm membrane in vitro

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Asian Journal of Andrology 2007年第1期9卷 77-81页

作者： Hua Qiang Ming-Sen jiang jian-yin lin Wei-Min He Department of Pathogenic Biology Etiology School of Medicine Wuhan University Wuhan 430071 China Department of Etiology 3Department of Anatomy Histology and Embryology Fujian Medical University Fuzhou 350004 China 不详　

Aim： To study the influence of enterococci on human sperm membrane in vitro. Methods： Ejaculated human sperm were artificially infected with β-hemolytic or non-β-hemolytic enterococci at the bacteria： sperm ratio of 50：1 at 37℃. Sperm membrane integrity was examined after incubation for 1, 3 and 5 h by hypoosmotic swelling （HOS） test and electron microscopy. Results： Sperm infected with β-hemolytic enterococci had lower HOS scores compared with non-β-hemolytic strains or uninfected control （P 〈 0.01）. The HOS test scores of sperm infected with β-hemolytic enterococci increased in the presence of phosphatidylcholine, an inhibitor of hemolysin. Non-β-hemolytic strains showed no significant difference in swelling rate, compared with the control group （P 〉 0.05）. It was shown by electron microscopy that β-hemolytic enterococci caused significant rupture of human sperm membrane. Conclusion： β-hemolytic enterococci caused human sperm membrane injury, and might be mediated by the hemolysin of enterococci.

关键词： enterococci sperm sperm membrane hypoosmotic swelling test ultrastructure

Prussian Blue Analogue‑Templated Nanocomposites for Alkali‑Ion Batteries:Progress and Perspective

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Nano-Micro Letters 2025年第1期17卷 216-261页

作者： jian‑En Zhou Yilin Li Xiaoming lin Jiaye Ye Key Laboratory of Theoretical Chemistry of Environment Ministry of EducationSchool of ChemistrySouth China Normal UniversityGuangzhou 510006People’s Republic of China School of Chemistry and Physics Faculty of ScienceQueensland University of Technology2 George StreetBrisbaneQLD 4000Australia Centre for Materials Science Queensland University of Technology2 George StreetBrisbaneQLD 4000Australia

Lithium-ion batteries(LIBs)have dominated the portable electronic and electrochemical energy markets since their commercialisation,whose high cost and lithium scarcity have prompted the development of other alkali-ion batteries(AIBs)including sodium-ion batteries(SIBs)and potassium-ion batteries(PIBs).Owing to larger ion sizes of Na^(+)and K^(+)compared with Li^(+),nanocomposites with excellent crystallinity orientation and well-developed porosity show unprecedented potential for advanced lithium/sodium/potassium *** enticing open rigid framework structures,Prussian blue analogues(PBAs)remain promising self-sacrificial templates for the preparation of various nanocomposites,whose appeal originates from the well-retained porous structures and exceptional electrochemical activities after thermal *** review focuses on the recent progress of PBA-derived nanocomposites from their fabrication,lithium/sodium/potassium storage mechanism,and applications in AIBs(LIBs,SIBs,and PIBs).To distinguish various PBA derivatives,the working mechanism and applications of PBA-templated metal oxides,metal chalcogenides,metal phosphides,and other nanocomposites are systematically evaluated,facilitating the establishment of a structure–activity correlation for these *** on the fruitful achievements of PBA-derived nanocomposites,perspectives for their future development are envisioned,aiming to narrow down the gap between laboratory study and industrial reality.

关键词： Prussian blue analogues Self-sacrificial template Lithium-ion batteries Sodium-ion batteries Potassium-ion batteries

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高精度干涉式光纤陀螺前置放大器的设计与实现

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中国惯性技术学报 2013年第5期21卷 668-671页

作者：鲁军殷建玲刘军李刚军械工程学院电子与光学工程系石家庄050003

针对高精度干涉式光纤陀螺方案的技术特点,为了提高陀螺精度,提出了基于直流对消与时域梳状滤波的前置放大器设计方法。首先在对光纤陀螺前放输入信号特征进行分析的基础上,针对信号微弱、信噪比低、包含有周期性的尖峰脉冲等特点,理论... 详细信息

针对高精度干涉式光纤陀螺方案的技术特点,为了提高陀螺精度,提出了基于直流对消与时域梳状滤波的前置放大器设计方法。首先在对光纤陀螺前放输入信号特征进行分析的基础上,针对信号微弱、信噪比低、包含有周期性的尖峰脉冲等特点,理论分析了前放信号的带宽、增益及低噪声等参数,提出了相应调理措施。其次依据调理措施,设计了采用直流对消、时域梳状滤波和两级放大滤波的宽带前放电路,并计算了相关参数。陀螺实验表明,该前放电路能将零漂在0.05(°)/h以上的陀螺降低为0.047(°)/h,证明设计的前放电路对于提高陀螺的精度指标是有效的。

关键词：光纤陀螺高精度前置放大直流对消时域梳状滤波

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Virulence and potential pathogenicity of coccoid Helicobacter pylori induced by antibiotics

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World Journal of Gastroenterology 2001年第2期7卷 254-258页

作者： Fei Fei She1 Dong Hui Su1 jian yin lin2 lin ying Zhou3 1Department of Microbiology, Fujian Medical University. Fuzhou 350004, Fujian Province, China2Department of Molecular Medicine, Fujian Medical University, Fuzhou 350004, Fujian Province, China 3Laboratory of Electron Microscope, Fujian Medical University, Fuzhou 350004. Fujian Province. ChinaFei Fei She. graduated from Fujian Medical University as a postgraduate in 1991, now associate professor of microbiology and immunology, specialized in molecular biology of pathogen, having 15 papers published. Department of Microbiology Fujian Medical University Fuzhou 350004 Fujian Province China.

AIM: To explore the virulence and the potential pathogenicity of coccoid Helicobacter pylori (H. pylori) transformed from spiral form by exposure to antibiotic. METHODS: Three strains of H. pylori, isolated from gastric biopsy specimens of confirmed peptic ulcer, were converted from spiral into coccoid from by exposure to metronidazole. Both spiral and coccoid form of H. pylori were tested for the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells, and the differences of the protein were analysed by SDS-PAGE and Western blot. The mutation of the genes including ureA, ureB,hpaA, vacA and cagA, related with virulence, was detected by means of PCR and PCR-SSCP. RESULTS: In the coccoid H. pylori,the urease activity, the adherence to Hep-2 cells and the vacuolating cytotoxicity to Hela cells all decreased. In strain F44, the rate and index of adherence reduced from 70.0% +/- 5.3% to 33% +/- 5.1% and from 2.6 +/- 0.4 to 0.96 +/- 0.3 (P < 0.01), respectively. The invasion of coccoid H. pylori into Hep-2 cell could be seen under electronmicroscope. SDS-PAGE showed that the content of the protein with the molecular weight over Mr 74000 decreased, and the hybriditional signal in band M(r) 125000 weakened, while the band M(r)110000 and M(r)63000 strengthened in coccoid *** as shown in Western blot. The results of PCR were all positive, and PCR-SSCP indicated that there may exist the point mutation in gene hpaA or vacA. CONCLUSION: The virulence and the proteins with molecular weight over M(r)74000 in coccoid *** decrease, but no deletion exists in amplification fragments from ureA, ureB, hpaA, vacA and cagA genes, suggesting that coccoid *** may have potential pathogenicity.

关键词： Antigens, Bacterial Adhesins, Bacterial Anti-Bacterial Agents Bacterial Proteins Blotting, Western Cell line Electrophoresis, Polyacrylamide Gel Helicobacter pylori Hemagglutinins Humans Metronidazole Mutation Polymerase Chain Reaction Polymorphism, Single-Stranded Conformational Research Support, Non-U.S. Gov't Urease Virulence

发酵莲子乳对小鼠胃肠道运动、吸收功能的调节作用

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世界华人消化杂志 2005年第21期13卷 2535-2539页

作者：吴小南陈洁汪家梨林建银福建医科大学公共卫生学院福建省福州市350004 福建医科大学分子医学研究中心福建省福州市350004

目的:探讨发酵莲子乳(LMFP)对胃肠道运动、吸收的调节作用.方法:小鼠按体质量区组随机分组并以蒸馏水、不同药物及不同浓度的LMFP进行相应干预.左旋精氨酸腹腔注射建模,测定胃及小肠酚红残留率及胃组织一氧化氮(NO)含量;复方地芬诺酯灌... 详细信息

目的:探讨发酵莲子乳(LMFP)对胃肠道运动、吸收的调节作用.方法:小鼠按体质量区组随机分组并以蒸馏水、不同药物及不同浓度的LMFP进行相应干预.左旋精氨酸腹腔注射建模,测定胃及小肠酚红残留率及胃组织一氧化氮(NO)含量;复方地芬诺酯灌胃制造便秘模型,观测炭末排出时间、黑便排出量、质量及含水率;皮下注射小剂量利血平建模,计算小肠推进率、尿木糖排出量.结果:胃组织NO含量与胃排空延缓正相关(r=0.475,P=0.010);较之左旋精氨酸模型组,250,500mL/LLMFP组、吗叮啉组胃酚红滞留率降低(22.78±6.95%,27.12±5.73%,22.82±5.63%vs34.76±9.15%,P<0.05),250mL/LLMFP组、吗叮啉组胃NO含量降低(26.13±4.95μmol/gpro,26.33±4.06μmol/gprovs38.33±9.82μmol/gpro,P<0.05),500,750mL/LLMFP组和吗叮啉组远端1/2小肠、750mL/LLMFP组和吗叮啉组远端2/3小肠酚红含量均增高(P<0.05).与复方地芬诺酯模型组相较,各浓度LMFP组炭末排出时间缩短(133.63±18.28,113.25±26.25min,141.75±25.95minvs175.50±24.04min,P<0.05)、黑便含水率升高(39.72±3.06%,42.48±3.07%,40.58±4.29%vs19.67±2.53%,P<0.05);250,500mL/LLMFP组黑便质量增加(0.68±0.16g,0.82±0.23gvs0.37±0.13g,P<0.01);1000mL/LLMFP组小肠推进率低于利血平模型组(79.93±14.52%vs97.64±5.68%,P=0.002),500,1000mL/LLMFP组木糖排出量较利血平模型组增高(10.54±2.48,12.24±2.15mgvs7.86±1.71mg,P<0.05).结论:LMFP对胃肠运动具有双相调节作用,可缓解便秘症状,促进小肠吸收功能.

关键词： LMFP对胃肠运动具有双相调节作用可缓解便秘症状促进小肠吸收功能

Dielectric-ion-conductive ZnNb2O6 layer enabling rapid desolvation and diffusion for dendrite-free Zn metal batteries

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Journal of Energy Chemistry 2025年第1期100卷 693-701页

作者： Haifeng Yang jian Wang Panpan Zhang Xiaomin Cheng Qinghua Guan Jing Dong Bixian Chen Lujie Jia Jing Zhang Yongzheng Zhang Yunjian Liu Hongzhen lin School of Materials Science and Engineering Jiangsu University i-Lab & CAS Key Laboratory of Nanophotonic Materials and Devices Suzhou Institute of Nano-Tech and Nano-Bionics Chinese Academy of Sciences Helmholtz Institute Ulm (HIU) Karlsruhe Institute of Technology (KIT) School of Materials Science and Engineering Xi'an University of Technology State Key Laboratory of Chemical Engineering East China University of Science and Technology

Rechargeable aqueous zinc-metal batteries （AZMBs） are promising candidates for large-scale energy storage systems due to their low cost and high ***,their performance and sustainability are significantly hindered by the sluggish desolvation kinetics at the electrode/electrolyte interface and the corresponding hydrogen evolution reaction where active water molecules tightly participate in the Zn（H2O）62+solvation ***,learnt from self-generated solid electrolyte interphase （SEI） in anodes,the dielectric but ion-conductive zinc niobate nanoparticles artificial layer is constructed on metallic Zn surface （ZNB@Zn）,acting as a rapid desolvation *** zincophilic and dielectric-conductive properties of ZNB layer accelerate interfacial desolvation/diffusion and suppress surface corrosion or dendrite formation,achieving uniform Zn plating/stripping behavior,as confirmed by electronic/optical microscopies and interface spectroscopical measurements together with theoretical ***,the as-prepared ZNB@Zn electrode exhibits excellent cycling stability of over 2000 h and robust reversibility （99.54%） even under high current density and depth of discharge ***,the assembled ZNB@Zn-based full cell displays high capacity-retention rate of 80.21%after 3000 cycles at 5 A g-1and outstanding rate performance up to 10 A *** large-areal pouch cell is stabilized for hundreds of cycles,highlighting the bright prospects of the dielectric but ion-conductive layer in further application of AZMBs.

关键词： Zn metal battery Dielectric artificial layer Rapid ion diffusion Zincophilic diffusion Active water inhibition

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基层医院聘用护士离职意愿及影响因素研究

中华护理杂志 2012年第6期47卷 534-536页

作者：李玲任忠玲段小芳殷健林江苏省溧水县中医院护理部 211200 溧水县卫生局医政科溧水县人民医院护理部

目的探讨基层医院聘用护士离职意愿及其影响因素。方法对某县一、二级医院271名聘用护士进行问卷调查。结果基层医院聘用护士离职意愿得分处于较高水平,且二级医院高于一级医院;基层医院聘用护士离职意愿影响因素包括组织承诺及工作环... 详细信息

目的探讨基层医院聘用护士离职意愿及其影响因素。方法对某县一、二级医院271名聘用护士进行问卷调查。结果基层医院聘用护士离职意愿得分处于较高水平,且二级医院高于一级医院;基层医院聘用护士离职意愿影响因素包括组织承诺及工作环境和工作性质。结论提高护士的组织承诺感,增加护理人力资源配置,改善工作环境,调整工作结构,可以降低聘用护士的离职意愿水平。

关键词：医院县护士离职意愿因素分析统计学

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Identification of a dual functional betulinic acid analog for the treatment of osteoarthritis by phenotypic screening

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Science China Life Sciences 2025年第1期68卷 300-302页

作者： Ze-Min lin Mai Xiang Wen-Hui Wei Shu-Hui Fan Li Chen Jie Wang Xiao-Qian Yang Chun-Hao Yang Shi-Jun He jian-Ping Zuo Laboratory of Immunopharmacology State Key Laboratory of Drug ResearchShanghai Institute of Materia MedicaChinese Academy of Sciences School of Pharmacy University of Chinese Academy of Sciences School of Pharmacy Nanjing University of Chinese Medicine State Key Laboratory of Drug Research Department of Medicinal ChemistryShanghai Institute of Materia MedicaChinese Academy of Sciences Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Experiment Center for Science and Technology Shanghai University of Traditional Chinese Medicine

Dear Editor, Phenotypic drug discovery, which observes compound effects on physiology,has given way to targeting specific molecules, but has revived by using modern methods to find drugs based on therapeutic outcomes in disease models(Vincent et al., 2022). Osteoarthritis(OA) is a prevalent chronic joint disorder, primarily affecting knee joints, with osteoclast activation due to low-grade inflammation resulting in increased bone resorption and structural changes in the joint(Katz et al.,2021).

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