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Analysis of Five Differentially Expressed Gene Families in Fast Elongating Cotton Fiber

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Acta Biochimica et Biophysica Sinica 2004年第1期36卷 51-56页

作者： jian-xun feng, Sheng-jian JI, Yong-Hui SHI, Yu XU, Gang WEI, and Yu-Xian ZHU( National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, Beijing 100871, China ) National Laboratory of Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 China

Using the suppression subtractive hybridization method, we isolated five gene families, including proline-rich proteins (PRPs), arabinogalactan proteins (AGPs), expansins, tubulins and lipid transfer proteins (LTPs), from fast elongating cotton fiber cells. Expression profile analysis using cDNA array technology showed that most of these gene families were highly expressed during early cotton fiber developmental stages (0-20 days post anthesis, DPA). Many transcripts accumulated over 50-fold in 10 DPA fiber cells than in 0 DPA samples. The entire gene family -AGP, together with 20 individual members in other 4 gene families, are reported in cotton for the first time. Accumulation of cell wall proteins, wall loosening enzymes, microtubules and lipid transfer proteins may contribute directly to the elongation and development of fiber cells.

关键词： cotton fiber cDNA array cell elongation arabinogalactan protein lipid transfer protein

Uncovering the oxidation mechanism of sphalerite(ZnS)in the absence and presence of water:A first-principles investigation

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International Journal of Mining Science and Technology 2025年第1期35卷 149-157页

作者： Yuanjia Luo Wei Sun Haisheng Han jian Peng feng jiang School of Minerals Processing and Bioengineering Central South UniversityChangsha 410083China

Herein,a first-principles investigation was innovatively conducted to research the surface oxidation of ZnS-like sphalerite in the absence and presence of H_(2)O .The findings showed that single O_(2) was preferred to be dissociated adsorption on sphalerite surface by generating SAO and Zn AO bonds,and the S atom on the surface was the most energy-supported site for O_(2) adsorption,on which a≡Zn-O-S-O-Zn≡structure will be ***,dissociated adsorption of single H_(2)O will not *** was preferred to be adsorbed on the top Zn atom on sphalerite surface in molecular form through Zn-O ***,sphalerite oxidation can occur as if O_(2) was present regardless of the presence of H_(2)O ,and when H_(2)O and O_(2) coexisted,the formation of sulfur oxide(SO_(2) )needed a lower energy barrier and it was easier to form on sphalerite surface than that only O_(2) *** the absence of H_(2)O ,when SO_(2) was generated,further oxidation of which would form neutral zinc *** the presence of H_(2)O ,the formation of SO_(2) on sphalerite surface was easier and the rate of further oxidation to form sulfate was also ***,the occurrence of sphalerite oxidation was accelerated.

关键词： First-principles Oxidation Sphalerite H_(2)O Lower energy barrier

A nuclide identification method ofγspectrum and model building based on the transformer

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Nuclear Science and Techniques 2025年第1期36卷 80-93页

作者： Fei Li Chu-Yang Luo Ying-Zi Wen Sheng Lv feng Cheng Guo-Qiang Zeng jian-feng jiang Bing-Hai Li College of Nuclear Technology and Automation Engineering Chengdu University of TechnologyChengdu 610000China Applied Nuclear Technology in Geosciences Key Laboratory of Sichuan Province Chengdu 610000China Department of Physics Universitat Politècnica de CatalunyaBarcelona 08028Spain Airborne Survey and Remote Sensing Center of Nuclear Industry Shijiazhuang 050000China Hebei Key Laboratory of Airborne Detection and Remote Sensing Technology Shijiazhuang 050000China

In current neural network algorithms for nuclide identification in high-background,poor-resolution detectors,traditional network paradigms including back-propagation networks,convolutional neural networks,recurrent neural networks,etc.,have been limited in research on γ spectrum analysis because of their inherent mathematical *** is difficult to make progress in terms of training data requirements and prediction *** contrast to traditional network paradigms,network models based on the transformer structure have the characteristics of parallel computing,position encoding,and deep stacking,which have enabled good performance in natural language processing tasks in recent ***,in this paper,a transformer-based neural network (TBNN) model is proposed to achieve nuclide identification for the first ***,the Geant4 program was used to generate the basic single-nuclide energy spectrum through Monte Carlo simulations.A multi-nuclide energy spectrum database was established for neural network training using random matrices of γ-ray energy,activity,and *** on the encoder–decoder structure,a network topology based on the transformer was built,transforming the 1024-channel energy spectrum data into a 32×32 energy spectrum sequence as the model *** experiments and adjustments of model parameters,including the learning rate of the TBNN model,number of attention heads,and number of network stacking layers,the overall recognition rate reached 98.7%.Additionally,this database was used for training AI models such as back-propagation networks,convolutional neural networks,residual networks,and long shortterm memory neural networks,with overall recognition rates of 92.8%,95.3%,96.3%,and 96.6%,*** indicates that the TBNN model exhibited better nuclide identification among these AI models,providing an important reference and theoretical basis for the practical application of transformers in the qualitative and quantitativ

关键词： Nuclide identification Neural network Transformer

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High Efficiency Mixed Amine Adsorbents for Directly Capturing Carbon Dioxide from Air

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Chemical Research in Chinese Universities 2025年第1期41卷 138-145页

作者： WU Yilu ZHENG Haolong XIA Zihan FU Chengmin XU feng YAN jian School of Environment and Chemical Engineering Foshan UniversityFoshan528000P.R.China Guangzhou Zhixin High School Guangzhou510080P.R.China

The development of effective adsorbents with high amine efficiency and CO_(2)adsorption almost unaffected by humidity is extremely *** this study,we introduce an innovative solid amine adsorbent,TETA/DEA@FS,composed of triethylenetetramine(TETA)and diethanolamine(DEA)functionalized fumed silica(FS),which exhibits exceptional capability in selectively capturing trace CO_(2)from N_(2).TETA/DEA@FS shows an exceptionally high capacity of CO_(2)adsorption of 1.13 mmol/g at the temperature of 298 K and the pressure of 0.0004 bar(1 bar=100 kPa),and achieves an unprecedented CO_(2)/N_(2)IAST selectivity of 1.70×10^(12).TETA/DEA@FS exhibits high amine efficiency,with breakthrough experiments demonstrating that CO_(2)adsorption remains nearly unaffected by ***,TETA/DEA@FS demonstrates rapid CO_(2)adsorption kinetics and outstanding cyclic stability.

关键词： Direct air capture(DAC) CO_(2) N_(2) Triethylenetetramine Diethanolamine

Hollow cerium nanoparticles synthesized by one-step method for multienzyme activity to reduce colitis in mice

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World Journal of Gastroenterology 2025年第5期31卷 94-104页

作者： Lin Mi Kai Zhang jian-Xia Ma jian-feng Yao Yi-Li Tong Zhi-Jun Bao Department of General Medicine Huadong HospitalFudan UniversityShanghai 200040China Department of Gastroenterology Huadong HospitalFudan UniversityShanghai 200040China

BACKGROUND Inflammatory bowel disease(IBD)is a common chronic intestinal inflammatory *** oxidative stress is a treatment target for *** oxide(CeO2)nanomaterials as nanozymes with antioxidant activity are potential drugs for the treatment of *** To synthesize hollow cerium(H-CeO2)nanoparticles by one-step method and to validate the therapeutic efficacy of H-CeO2 in *** H-CeO2 was synthesized by one-step method and examined its characterization and nanoenzymatic ***,we constructed dextran sulfate so-dium(DSS)-induced colitis in mice to observe the effects of H-CeO2 on colonic *** effects of H-CeO2 on colon inflammation and reactive oxygen species(ROS)levels in IBD mice were detected by hematoxylin and eosin staining and dichlorofluorescein diacetate staining,***,the biological sa-fety of H-CeO2 on mice was evaluated by hematoxylin and eosin staining,blood routine,and blood *** H-CeO2 nanoparticles prepared by the one-step method were uniform,monodi-sperse and hollow.H-CeO2 had a good ability to scavenge ROS,∙OH and∙OOH.H-CeO2 reduced DSS-induced decreases in body weight and colon length,colonic epithelial damage,inflammatory infiltration,and ROS accumulation.H-CeO2 administration reduced the disease activity index of DSS-induced animals from about 8 to 5.H-CeO2 had no significant effect on body weight,total platelet count,hemoglobin,white blood cell,and red blood cell counts in healthy *** significant damage to major organs was observed in healthy mice following H-CeO2 *** The one-step synthesis of H-CeO2 nanomaterials had good antioxidant activity,biosafety,and inhibited deve-lopment of DSS-induced IBD in mice by scavenging ROS.

关键词： Inflammatory bowel disease Nanozymes Hollow cerium nanoparticles One-step method Cerium oxide biosafety

Liver transplantation using an otherwise-wasted partial liver resection graft

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Hepatobiliary & Pancreatic Diseases International 2025年第1期24卷 29-34页

作者： Yong-Sheng Xiao Yi-feng He Xiao-Wu Huang Zhao-You Tang Jia Fan jian Zhou Department of Liver Surgery&Transplantation Zhongshan HospitalFudan UniversityShanghai 200032China

Liver transplantation represents a complex surgical procedure and serves as a curative treatment for patients presenting an acute or chronic end-stage liver disease, or carefully selected liver malignancy. A significant gap still exists between the number of available donor organs and potential recipients. The use of an otherwise-wasted resected liver lobe from patients with benign liver tumors is a new, albeit small, option to alleviate the allograft shortage. This review provides evidence that resected liver lobes may be used successfully in liver transplantation.

关键词： Otherwise-wasted resected liver lobe Partial liver resection graft Liver transplantation Alternative liver transplantation technique Liver resection Benign hepatic tumor Hemangioma Focal nodular hyperplasia

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In situ measurement of environmental γ radiation dose rates of key nuclides for large radioactive surface sources

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Nuclear Science and Techniques 2025年第1期36卷 69-79页

作者： Ze-Qian Wu jian Sun Wei-Qi Huang Bai-Rong Wang Jin-Xing Cheng jiang-feng Wu Yong-Hong Wang Biao Yuan Sheng Qi Kun Shan Institute of NBC Defence Beijing 102205China Beijing New-High Technology Academy Beijing 100094China

To monitor nuclear and radiation emergencies,it is crucial to obtain accurate in situ measurements of the environmentalγ radiation dose rate from key radionuclides,particularly for large radioactive surface *** methods currently used for measuring dose rates are inadequate for obtaining the dose rates of key radionuclides and have large angular response errors when monitoring surface *** address this practical problem,this study proposes three methods for measuring the dose rate:the weighted peak total ratio,mean value regression,and numerical integration *** methods are based on energy-spectrum measurement data,and they were theoretically derived and numerically ***,a 1-m-long hexagonal radioactive surface source was integrated into a larger surface *** situ measurement experiments were conducted on a large radioactive surface source using a dose-rate meter and a portable HPGespectrometer to analyze the errors of the three aforementioned methods and verify their validity.

关键词： Environmentalγradiation dose rate HPGespectrometer Key radionuclides Radioactive surface source

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A novel active-passive protection strategy endows carbonyl iron with excellent intelligent anti-corrosion and microwave absorption dual functional characteristics

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Nano Research 2025年第1期18卷 487-500页

作者： Wei Tian Quanyuan feng Linbo Zhang Yinfan Liu Xian jian Longjiang Deng Institute of Microelectronics School of Information Science and TechnologySouthwest Jiaotong UniversityChengdu 611756China National Engineering Researching Centre of Electromagnetic Radiation Control Materials Key Laboratory of Multi-Spectral Absorbing Materials and Structures of Ministry of EducationState Key Laboratory of Electronic Thin Films and Integrated DevicesSchool of Electronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu 611731China School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China The Yangtze Delta Region Institute(Huzhou)&School of Electronic Science and Engineering University of Electronic Science and Technology of ChinaHuzhou 313001China

Carbonyl iron absorbers(CI)face significant challenges in practical applications,such as corrosion,interface bonding failure,detachment,and high maintenance ***,we have developed intelligent self-healing technology based on proactive/passive mechanisms via in situ synthesis of self-healing factors(polydopamine/benzotriazole(PDA/BTA))and physical barrier layers(SiO_(2)/1,1,1,3,3,3-hexamethyl disilazane,SiO_(2)/HMDS)to enhance corrosion resistance,while also being compatible with efficient microwave absorption *** unique multiscale structure gives full play to the utilization of the roles of each functional layer,including the intelligent self-healing features of PDA/BTA,physical shielding and spatial confinement characteristics of SiO_(2)/HMDS,and magnetic-dielectric synergistic mechanism resulted from the good impedance matching characteristics,the conduction loss,the interfacial polarization loss and natural *** asfabricated composites achieved an exceptional minimum reflection loss value of-55.4 dB at 10.7 GHz and the effective absorption band of 7.6 ***,it still exhibits obvious self-healing and corrosion resistance characteristics after 360 h corrosion treatment,ascribed to the self-healing mechanism of PDA/BTA and the blocking intervention effect of SiO_(2)/*** work is considered to pave the way for the synthesis of high-performance magnetic absorbers,especially in enhancing their intelligent self-healing ability in corrosive environments.

关键词： microwave absorption intelligent self-healing technology carbonyl iron multiscale structure magneticdielectric synergistic mechanism

SD大鼠羊水间充质干细胞向神经样细胞体外诱导分化

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世界华人消化杂志 2010年第3期18卷 250-255页

作者：冯建勋腊晓林马艳毕晓娟温浩新疆医科大学第一附属医院医学研究中心干细胞研究所新疆维吾尔自治区乌鲁木齐市830000 新疆医科大学第一附属医院博士后科研流动站新疆乌鲁木齐市友谊医院肾内科新疆维吾尔自治区乌鲁木齐市830000 新疆医科大学第一附属医院妇产科新疆维吾尔自治区乌鲁木齐市830000 新疆医科大学第一附属医院肝胆外科新疆维吾尔自治区乌鲁木齐市830000

目的:探讨体外诱导羊水间充质干细胞(AF-MSCs)分化成神经干细胞.方法:10只SD怀孕大鼠,180-240g,胎龄16d,过量腹腔麻醉致死,开腹全部切除子宫,在无菌的条件下抽取全羊水,采用贴壁法分离AF-MSCs,体外培养扩增,观察细胞生长特性,流式细胞... 详细信息

目的:探讨体外诱导羊水间充质干细胞(AF-MSCs)分化成神经干细胞.方法:10只SD怀孕大鼠,180-240g,胎龄16d,过量腹腔麻醉致死,开腹全部切除子宫,在无菌的条件下抽取全羊水,采用贴壁法分离AF-MSCs,体外培养扩增,观察细胞生长特性,流式细胞仪检测AF-MSCs表面抗原表达及细胞周期.取第3代AF-MSCs,加入预诱导液(DMEM/F12、100mL/LFBS、1mmol/Lβ-MEM)诱导24h,然后加入诱导液(DMEM/F12、100mL/LFBS、5mmol/Lβ-MEM)诱导24h,最后在分化维持培养液(DMEM/F12、20μg/LEGF、20μg/L bFGF)中培养,行免疫荧光染色,检测NSE和GFAP的表达.结果:成功分离、培养和传代SD大鼠AF-MSCs,流式细胞术检测提示大鼠AF-MSCs的G0/G1期细胞比例约为87.5%、S+G2+M期比例为12.5%,表达CD44为98.3%、CD29为70%、和CD105为33.2%、不表达CD45为0.5%,CD34为1%,DLA-DR(MHCclassⅡ)为0.1%.诱导后,AF-MSCs能表达神经细胞标示物NSE和GFAP,且可在体外继续存活2wk左右.结论:羊水中也存在MSCs,与其他来源的MSCs特点相符;羊水可以作为一种新的胎儿MSCs来源,而且能在体外诱导分化为神经细胞.

关键词：羊水细胞间充质干细胞神经样细胞大鼠流式细胞术免疫细胞化学碱性成纤维细胞生长因子重组表皮生长因子