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Chinese Annals of Mathematics,Series B 2024年第5期45卷 805-822页

作者： jian-xin liu Zhi-qiang GAO School of Mathematical Sciences Beijing Normal UniversityBeijing 100875China Laboratory of Mathematics and Complex Systems(Ministry of Education) School of Mathematical SciencesBeijing Normal UniversityBeijing 100875China

Consider a branching random walk with a random environment in time in the d-dimensional integer *** branching mechanism is governed by a supercritical branching process,and the particles perform a lazy random walk with an independent,non-identical increment *** A■Z^(d),let Z_(n)(A)be the number of offsprings of generation n located in *** exact convergence rate of the local limit theorem for the counting measure Z_(n)(·)is *** partially extends the previous results for a simple branching random walk derived by Gao(2017,*** Appl.).

关键词： Branching random walk Random environment Local limit theorems Exact convergence rate

Feedomics provides bidirectional omics strategies between genetics and nutrition for improved production in cattle

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Animal Nutrition 2022年第2期9卷 314-319页

作者： Xiaohan liu Yifan Tang Jiajin Wu jian-xin liu Hui-Zeng Sun Institute of Dairy Science MoE Key Laboratory of Molecular Animal NutritionCollege of Animal SciencesZhejiang UniversityHangzhouChina

Increasing the efficiency and sustainability of cattle production is an effective way to produce valuable animal proteins for a growing human *** and nutrition are the 2 major research topics in selecting cattle with beneficial phenotypes and developing genetic potentials for improved *** is an inextricable link between genetics and nutrition,which urgently requires researchers to uncover the underlying molecular mechanisms to optimize cattle *** integrates a range of omic techniques to reveal the mechanisms at different molecular levels related to animal production and health,which can provide novel insights into the relationships of genes and nutrition/*** this review,we summarized the applications of feedomics techniques to reveal the effect of genetic elements on the response to nutrition and investigate how nutrients affect the functional genome of cattle from the perspective of both nutrigenetics and *** highlighted the roles of rumen microbiome in the interactions between host genes and ***,we discuss the importance of feedomics in cattle nutrition research,with a view to ensure that cattle exhibit the best production traits for human consumption from both genetic and nutritional aspects.

关键词： Cattle Feedomics Nutrigenetics Nutrigenomics Rumen microbiome Production

Assessment of the hepatic microvascular changes in liver cirrhosis by perfusion computed tomography

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World Journal of Gastroenterology 2009年第28期15卷 3532-3537页

作者： Mai-Lin Chen Qing-Yu Zeng jian-Wei Huo Xiao-Ming Yin Bao-Ping Li jian-xin liu Department of Radiology ChinaMeitan General Hospital No. 29 Xibahe Nanli ChaoyangDistrict Beijing 100028 China

AIM： TO assess the hepatic microvascular parameters in patients with liver cirrhosis by perfusion computed tomography （CT）. METHODS： Perfusion CT was performed in 29 patients without liver disease （control subjects） and 39 patients with liver cirrhosis, including 22 patients with compensated cirrhosis and 17 patients with decompensated cirrhosis, proved by clinical and laboratory parameters. CT cine-scans were obtained over 50 s beginning with the injection of 50 mL of contrast agent. Hepatic microvascular parameters, mean transit time （MTT） and permeability surface area product （PS） were obtained with the Perfusion 3 software （General Electric, ADW 4.2）. RESULTS： The overall differences of MTT and PS between control subjects, patients with compensated cirrhosis and those with decompensated cirrhosis were statistically significant （P = 0.010 and P = 0.002, respectively）. MTT values were 15.613 ± 4.1746 s, 12.592 ± 4.7518 s, and 11.721 ± 4.5681 s for the three groups, respectively, while PS were 18.945 ± 7.2347 mL/min per 100 mL, 22.767 ± 8.3936 mL/min per 100 mL, and 28.735 ± 13.0654 mL/min per 100 mL. MTT in decompensated cirrhotic patients were significantly decreased compared to controls （P = 0.017）, whereas PS values were remarkably increased （P = 0.001）.CONCLUSION： The hepatic microvascular changes in patients with liver cirrhosis can be quantitatively assessed by perfusion CT. Hepatic microvascular parameters （MTr and PS）, as measured by perfusion CT, were significantly altered in decompensated cirrhosis.

关键词： Tomography X-ray computed Hepaticmicrocirculation Cirrhosis

Effect of ischemic postconditioning on cerebral edema and the AQP4 expression following hypoxic-eschemic brain damage in neonatal rats

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World Journal of Pediatrics 2015年第2期11卷 165-170页

作者： Ling Yu Shu-Juan Fan Li liu Mi Xiao Xiao-Jie Lin Yong liu Hai-Xia Lv xin-Lin Chen jian-xin liu Department of Neonatology First Affiliated HospitalXi'an Jiaotong University School of MedicineXi'anChina Department of Nephrology Children's HospitalZhejiang University School of MedicineChina Institute of Neurobiology of Xi'an Jiaotong University School of Medicine Xi'anChina

Background:A rat model for neonatal hypoxic-ischemic brain damage(HIBD)was established to observe the effect of ischemic postconditioning(IPostC)on cerebral edema and the AQP4 expression following HIBD and to verily the neuroprotection of IPostC and the relationship between changes of AQP4 expression and cerebral ***:Water content was measured with dry-wet method,and AQP4 transcription and the protein expression of the lesions were detected with real-time PCR and immunohistochemistry staining,***:Within 6-48 hours,the degree of ipsilateral cerebral edema was significantly lower in IPostC-15 s/15 s group than in HIBD *** to the HIBD group,the AQP4 transcription and expression in the IPostC group showed a downward and then upward *** the expression was still more evident in the HIBD group than in the IPostC-15 s/15 s *** 24 to 48 hours,IPostC-15 s/15 s decreased the slowing down expression of ***:IPostC has neuroprotective effect on neonatal rats with HIBD and it may relieve cerebral edema by regulating the expression of AQP4.

关键词： AQP4 cerebral edema hypoxic-ischemic brain injury ischemic postconditioning neonate

Cloning and functional analysis of a novel ascorbate peroxidase(APX) gene from Anthurium andraeanum

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1110-1120页

作者： Hui-chun liu Dan-qing TIAN jian-xin liu Guang-ying MA Qing-cheng ZOU Zhu-jun ZHU College of Agriculture & Biotechnology Zhejiang University Research & Development Center of Flower Zhejiang Academy of Agricultural Sciences School of Agricultural and Food Science Zhejiang A&F University

An 888-bp ful-length ascorbate peroxidase （APX） complementary DNA （cDNA） gene was cloned from Anthurium andraeanum, and designated as AnAPX. It contains a 110-bp 5′-noncoding region, a 28-bp 3′-noncoding region, and a 750-bp open reading frame （ORF）. This protein is hydrophilic with an aliphatic index of 81.64 and its structure consisting ofα-helixes,β-turns, and random coils. The AnAPX protein showed 93%, 87%, 87%, 87%, and 86% similarities to the APX homologs from Zantedeschia aethiopica, Vitis pseudoreticulata, Gossypium hirsutum, Elaeis guineensis, and Zea mays, respectively. AnAPX gene transcript was measured non-significantly in roots, stems, leaves, spathes, and spadices by real-time polymerase chain reaction （RT-PCR） analysis. Interestingly, this gene expression was remarkably up-regulated in response to a cold stress under 6 °C, implying that AnAPX might play an important role in A. andraeanum tolerance to cold stress. To confirm this function we overexpressed AnAPX in tobacco plants by transformation with an AnAPX expression construct driven by CaMV 35S promoter. The transformed tobacco seedlings under 4 °C showed less electrolyte leakage （EL） and malondialdehyde （MDA） content than the control. The content of MDA was correlated with chilling tolerance in these transgenic plants. These results show that AnAPX can prevent the chilling challenged plant from cellmembrane damage and ultimately enhance the plant cold tolerance.

关键词： AnAPX Gene expression Cold stress Anthurium andraeanum

Age-and Microbiota-Dependent Cell Stemness Plasticity Revealed by Cattle Cell Landscape

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Research 2023年第3期6卷 345-360页

作者： Jia-Jin Wu Senlin Zhu Yi-Fan Tang Fengfei Gu Teresa GValencak jian-xin liu Hui-Zeng Sun Institute of Dairy Science College of Animal SciencesZhejiang UniversityHangzhou 310058China Ministry of Education Key laboratory of Molecular Animal Nutrition Zhejiang UniversityHangzhou 310058China Ministry of Education Innovation Team of Development and Function of Animal Digestive System Zhejiang UniversityHangzhou 310058China

Newborn ruminants are considered functionally monogastric *** poor understanding of cellular differences between newborn and mature ruminants prevents the improvement of health and performance of domestic ***,we performed the single-cell RNA sequencing on the rumen,reticulum,omasum,abomasum,duodenum,jejunum,ileum,cecum,colon,rectum,liver,salivary gland,and mammary gland from newborn and adult cattle.A comprehensive single-cell transcriptomic atlas covering 235,941 high-quality single cells and 78 cell types was deciphered.A Cattle Cell Landscape database(http://***)was established to elaborately display the data and facilitate effective annotation of cattle cell types and subtypes for the broad research *** measuring stemness states of epithelial cells in each tissue type,we revealed that the epithelial cells from newborn forestomach(rumen,reticulum,and omasum)were more transcriptionally indistinct and stochastic compared with the adult stage,which was in contrast to those of abomasum and intestinal *** rapid forestomach development during the early life of calves was driven by epithelial progenitor-like cells with high DNA repair activities and ***,in the forestomach tissues of newborn calves,the Megasphaera genus was involved in regulating the transcriptional plasticity of the epithelial progenitor-like cells by DNA methylation regulation.A novel cell type,the STOML3+cell,was found to be *** apparently plays a crucial role in stemness maintenance of its own and cholangiocytes in the hepatic *** results reveal that the age-and microbiota-dependent cell stemness plasticity drives the postnatal functional maturity of ruminants.

关键词： stomach maturity cattle

Carbon quantum dots/Bi_(4)O_(5)Br_(2) photocatalyst with enhanced photodynamic therapy:killing of lung cancer(A549)cells in vitro

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Rare Metals 2022年第1期41卷 132-143页

作者： Bing He Hai-Yan Jin Ya-Wen Wang Cai-Mei Fan Yun-Fang Wang Xiao-Chao Zhang jian-xin liu Rui Li Jue-Wen liu College of Chemistry and Chemical Engineering Taiyuan University of TechnologyTaiyuan 030024China Department of Chemistry University of WaterlooWaterlooON N2L 3G1Canada

Inorganic photocatalysts have been regarded as a promising candidate in the domain of tumor photodynamic therapy(PDT)due to their inspirational photocatalytic *** this study,a Bi_(4)O_(5)Br_(2)photocatalyst was synthesized and it exhibited effective photo-killing activity of A549 cells(a human lung carcinoma epithelial cell line)in *** this basis,we modified Bi_(4)O_(5)Br_(2)with carbon quantum dots(CQDs)via a hydrolysis method at room temperature,which resulted in an improved photo-killing effect of Bi_(4)O_(5)Br_(2)to A549 *** samples and the interaction between samples and cells were fully *** has been found that the loading of CQDs on Bi_(4)O_(5)Br_(2)can reduce the hydration ratio,increase the cellular uptake and improve the photogenerated reactive oxygen species(ROS)as compared with pristine Bi_(4)O_(5)Br_(2).Electron spin resonance(ESR)analysis and radical-trapping experiments manifested that the ROS contributed to PDT may be·O^(2-)and ·*** study may provide a useful strategy to ameliorate the penetrability,cell compatibility and PDT effect upon cancer cells of other inorganic photocatalysts.

关键词： CQDs/Bi_(4)O_(5)Br_(2) Photocatalyst PDT A549 cell Reactive oxygen species

Stereoselective　Syntheses　of　Some　β-D-1-Thioglycopyranosides　and　Bis(β-D-glycopyranosyl)　Sulfides　by　Phase　Transition　Catalysis

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Chemical Research in Chinese Universities 1997年第1期13卷 23-28页

作者： YU jian xin, liu Fang min, SUN Wan fu and liu Yu ting (Department of Chemistry, xinjiang University, Ulumuqi, 830046) xinjianG UNIV DEPT CHEMULUMUQI 830046PEOPLES R CHINA

The present paper covers the stereoselective syntheses of four 1 thiogalactopyranosides 1—4 and three thio disaccharides 5—7 through the PTC methods and their structures confirmed by means of elementary analysis, ... 详细信息

关键词： Synthesis Phase transition catalysis Thiogalactopyranoside Thio disaccharide Stereospecificity

Qingjie Fuzheng granules inhibit colorectal cancer cell growth by the PI3K/AKT and ERK pathways

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World Journal of Gastrointestinal Oncology 2019年第5期11卷 377-392页

作者： Hong Yang jian-xin liu Hai-Xia Shang Shan Lin Jin-Yan Zhao Jiu-Mao Lin Academy of Integrative Medicine Fujian University of Traditional Chinese Medicine Fujian Key Laboratory of Integrative Medicine on Geriatrics Fujian University of Traditional Chinese Medicine

BACKGROUND Qingjie Fuzheng granules(QFGs) are part of a traditional Chinese medicine formula, which has been widely used and found to be clinically effective with few side effects in various cancer treatments, including colorectal cancer(CRC).However, the precise mechanisms and molecular signaling pathways involved in the activity of QFGs' anticancer effect have not been reported in the literature. In this study, we hypothesized that QFGs can inhibit the growth of colorectal cancer cells, and that its mechanism is closely related to one or more intracellular signal transduction *** To better evaluate the mechanism underlying the anti-cancer effect of QFGs on the CRC cell lines HCT-116 and *** First, we measured cell viability and cytotoxicity by performing MTT and lactate dehydrogenase(LDH) assays. We evaluated the role of QFGs in cell proliferation and apoptosis by assessing colony formation and analyzing Hoechst 33258 staining. Second, cell cycle and apoptosis rates were measured by fluorescence activated cell sorting, and the expression levels of survivin, cyclin D1, CDK4, p21,Bax, Bcl-2, Fas, FasL, and cleaved-caspase-3/-8/-9 were measured by performing western blots and caspase activity assays. Furthermore, inhibitors of caspase-3/-8/-9 were used to elucidate the specific apoptosis pathway induced by QFGs in cancer cells. Finally, activation of the PI3 K/AKT and ERK signaling pathwayswas examined using the western blot assay to investigate the possible *** MTT and LDH assays revealed that after 0.5-2.0 mg/mL of QFGs treatment, cell viability was reduced by(6.90% ± 1.03%)–(59.70% ± 1.51%)(HCT-116; P < 0.05)and(5.56% ± 4.52%)–(49.44% ± 2.47%)(HCT-8; P < 0.05), and cytotoxicity was increased from 0.52 ± 0.023 to 0.77 ± 0.002(HCT-116; P < 0.01) and from 0.56 ±0.054 to 0.81 ± 0.044(HCT-8; P < 0.01) compared with the non-QFGs treatment groups. Additionally, colony formation and Hoechst 33258 staining assays showed that QFGs inhibited p

关键词： Qingjie Fuzheng granules Colorectal cancer Proliferation Apoptosis PI3K/AKT ERK

Comparative studies of two methods for miRNA isolation from milk whey

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2015年第6期16卷 533-540页

作者： Xiao-lu JIN Zi-hai WEI Lan liu Hong-yun liu jian-xin liu Institute of Dairy Science MoEKey Laboratory of Molecular Animal NutritionCollege of Animal SciencesZhejiang University

Micro RNAs（mi RNAs） from milk whey have been considered for their potential as noninvasive biomarkers for milk quality control and disease diagnosis. However, standard protocols for mi RNA isolation and quantification from milk whey are not well established. The objective of this study was to compare two methods for the isolation of mi RNAs from milk whey. These two methods were modified phenol-based technique（Trizol LS followed by phenol precipitation, the TP method） and combined phenol and column-based approach（Trizol LS followed by cleanup using the mi RNeasy kit, the TM method）. Yield and quality of RNA were rigorously measured using a Nano Drop ND-1000 spectrophotometer and then the distribution of RNA was precisely detected in a Bioanalyzer 2100 instrument by microchip gel electrophoresis. Several endogenous mi RNAs（bta-mi R-141, bta-mi R-146 a, bta-mi R-148 a, bta-mi R-200 c, bta-mi R-362, and bta-mi R-375） and an exogenous spike-in synthetic control mi RNA（cel-mi R-39） were quantified by real-time polymerase chain reaction（PCR） to examine the apparent recovery efficiency of milk whey mi RNAs. Both methods could successfully isolate sufficient small RNA（200 nt） from milk whey, and their yields were quite similar. However, the quantification results show that the total mi RNA recovery efficiency by the TM method is superior to that by the TP method. The TM method performed better than the TP for recovery of milk whey mi RNA due to its consistency and good repeatability in endogenous and spike-in mi RNA recovery. Additionally, quantitative recovery analysis of a spike-in mi RNA may be more accurate to reflect the milk whey mi RNA recovery efficiency than using traditional RNA quality analysis instruments（Nano Drop or Bioanalyzer 2100）.

关键词： Method Milk whey mi RNA Spike-in miRNA