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甲硫氨酸限制对骨肉瘤细胞增殖、凋亡及铁死亡的影响

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现代生物医学进展 2024年第9期24卷 1608-1613,1638页

作者：张馨丹赵正刚张文轩李芳红周素瑾赵子建广东工业大学生物医药学院广东广州510006

目的:探讨甲硫氨酸限制对骨肉瘤细胞增殖,凋亡和铁死亡的影响。方法:使用细胞计数检测剥夺甲硫氨酸对三株不同的骨肉瘤细胞系增殖的影响;高通量测序(RNA-seq)分析甲硫氨酸限制后骨肉瘤细胞的转录组学变化;流式细胞术检测细胞周期,凋亡以... 详细信息

目的:探讨甲硫氨酸限制对骨肉瘤细胞增殖,凋亡和铁死亡的影响。方法:使用细胞计数检测剥夺甲硫氨酸对三株不同的骨肉瘤细胞系增殖的影响;高通量测序(RNA-seq)分析甲硫氨酸限制后骨肉瘤细胞的转录组学变化;流式细胞术检测细胞周期,凋亡以及ROS,脂质ROS水平;Western blot检测铁死亡关键蛋白谷胱甘肽过氧化物GPX4以及铁死亡标志蛋白前列腺素内过氧化物合成Cox-2的表达。结果:甲硫氨酸限制显著抑制骨肉瘤细胞的增殖(P<0.001);RNA-seq分析筛选出1719个差异表达基因,基因富集分析发现甲硫氨酸限制可显著激活铁死亡通路并显著抑制细胞周期通路;细胞实验证实甲硫氨酸限制将骨肉瘤细胞阻滞在G2M期,显著诱导凋亡细胞比例增加;同时,细胞内Cox-2的表达增加,GPX4的活性降低,活性氧和脂质活性氧积累,最终导致细胞死亡,且这种作用可以被铁死亡抑制剂Fer-1部分挽救。结论:本研究的结果为甲硫氨酸限制治疗骨肉瘤提供了一定的科学依据。

关键词：甲硫氨酸限制骨肉瘤增殖铁死亡

Loading single lanthanide ion into aluminum molecular rings:water-stable sodalite cage for removal of nuclear-industry anions

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Science China Chemistry 2023年第5期66卷 1384-1393页

作者： Yi-Fan Sun Ya-Jie Liu San-Tai Wang Xiao-Yu Liu Chao Ma Wei-Hui Fang jian zhang State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of MatterChinese Academy of SciencesFuzhou 350002China

Based on our discovery of neutral aluminum molecular rings,we herein introduce rare earth ions into the center of the ring and reveal their structural adaptability.The resulting yoyo-like cationic macrocycles are highly adaptive to guests,creating unusual supramolecular assemblies.The first category is stacked with singly oriented rhombohedral channels,which exhibit fast and reversible solvent-triggered molecular motions and structural rearrangements.The second type of assembly is that yoyo macrocycles and guest molecules form a nested host-guest sodalite cage supramolecular structure.It is uncommon for guest molecules to be well-defined within cages although caged structures have been well-documented.Their host-guest interaction discussion reveals the self-adaptation and flexibility of the yoyo macrocycles.Considering their good water stability,we investigated their anion exchange properties.The results show that they have significant exchange capacity for KI/I_(2),ReO_(4)^(-),and MnO_(4)^(-),revealing their potential application in water purification and nuclear waste treatment.

关键词： aluminum molecular ring lanthanide structural adaptability sodalite cage iodine capture

Photodegradation of perfluorooctanoic acid by 185 nm vacuum ultraviolet light

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Journal of Environmental Sciences 2007年第4期19卷 387-390页

作者： CHEN Jing zhang Peng-yi LIU jian Department of Environmental Science and Engineering Tsinghua University Beijing 100084 China.

The photodegradation of persistent and bioaccumulative perftuorooctanoic acid （PFOA） in water by 185 nm vacuum ultraviolet （VUV） light was examined to develop an effective technology to deal with PFOA pollution. PFOA degraded very slowly under irradiation of 254 nm UV light. However, 61.7% of initial PFOA was degraded by 185 nm VUV light within 2 h, and defluorination ratio reached 17.1%. Pseudo first-order-kinetics well simulated its degradation and defluorination. Besides, fluoride ion formed in water, 4 shorter-chain perfluorinated carboxylic acids （PFCAs）, that is, perfluoroheptanoic acid, perfluorohexanoic acid, perfluoropentanoic acid, and perfluorobutanoic acid. These were identified as intermediates by LC-MS measurement. These PFCAs consecutively formed and further degraded with irradiation time. According to the mass balance calculation, no other byproducts were formed. It was proposed that PFCAs initially are decarboxylated by 185 nm light, and the radical thus formed reacts with water to form shorter-chain PFCA with one less CF2 unit.

关键词： photodegradafion perfluorooctanoic acid perfluorinated carboxylic acid vacuum ultraviolet

Development of a cELISA for effective detection of the antibody against H7 subtype of avian influenza virus

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Journal of Integrative Agriculture 2022年第1期21卷 199-207页

作者： WANG Cong-cong WANG Si-wen zhang Ying SHI jian-zhong YIN Xin LI Cheng-jun WANG Xiu-rong State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069P.R.China shenyang Institute of Technology Shenyang 110870P.R.China

H7 avian influenza viruses(AIVs) normally circulated among birds before. From 1996 to 2012, human infections with H7 AIVs(H7 N2, H7 N3, and H7 N7) were reported in Canada, Italy, Mexico, the Netherlands, the United Kingdom and the USA. Until March 2013, human infections with H7 N9 AIVs were reported in China. Since then, H7 N9 AIVs have continued to circulate in both humans and birds. Therefore, the detection of antibodies against the H7 subtype of AIVs has become an important topic. In this study, a competitive enzyme-linked immunosorbent assay(cELISA)method for the detection of antibody against H7 AIVs was established. The optimal concentration of antigen coating was 5 μg mL^(-1), serum dilution was 1/10, and enzyme-labeled antibody was 1/3 000. To determine the cut-off value of cELISA, percent inhibition(PI) was determined by using receiver operating characteristic(ROC) curve analysis in 178 AIVs negative samples and 368 AIVs positive serum samples(n=546). When PI was set at 40%, the specificity and sensitivity of cELISA were 99.4 and 98.9%, respectively. This method could detect the antibodies against H7 Nx(N1–N4, N7–N9) AIVs, and showed no reaction with AIVs of H1–H6 and H8–H15 subtypes or common avian viruses such as Newcastle disease virus(NDV), Infectious bronchitis virus(IBV) and Infectious bursal disease virus(IBDV), exhibiting good specificity. This method showed a coincidence rate of 98.56% with hemagglutinin inhibition(HI) test. And the repeatability experiment revealed that the coefficients of variation(CV) of intra-and inter-batch repetition were all less than 12%. The data indicated that the cELISA antibody-detection method established in this study provided a simple and accurate technical support for the detection of a large number of antibody samples of H7-AIV.

关键词： H7 subtype influenza monoclonal antibody cELISA

振幅整合脑电图评分联合ox-LDL/HDL比值、hs-CRP/Alb比值、FAR对急性脑梗死患者预后的预测价值

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现代生物医学进展 2024年第12期24卷 2391-2395+2382页

作者：张艳阳蔡建叶邱玉坤杨娟唐玉彬中国人民解放军联勤保障部队第九四〇医院急诊医学科

目的：探讨振幅整合脑电图（aEEG）评分联合氧化低密度脂蛋白/高密度脂蛋白（ox-LDL/HDL）比值、高敏C反应蛋白/白蛋白（hs-CRP/Alb）比值、纤维蛋白原/白蛋白比值（FAR）对急性脑梗死（ACI）患者预后的预测价值。方法：选择2021年1月至... 详细信息

目的：探讨振幅整合脑电图（aEEG）评分联合氧化低密度脂蛋白/高密度脂蛋白（ox-LDL/HDL）比值、高敏C反应蛋白/白蛋白（hs-CRP/Alb）比值、纤维蛋白原/白蛋白比值（FAR）对急性脑梗死（ACI）患者预后的预测价值。方法：选择2021年1月至2022年l2月我院收治的242例ACI患者，均行aEEG检查获得aEEG评分，检测血清ox-LDL/HDL比值、hs-CRP/Alb比值、FAR。出院3个月后采用改良Rankin量表（m RS）评估患者预后。收集ACI患者的临床资料，多因素Logistic回归分析ACI患者预后不良的影响因素。受试者工作用特征（ROC）曲线分析aEEG评分、血清ox-LDL/HDL比值、hs-CRP/Alb比值、FAR预测ACI患者预后的效能。结果：预后不良组aEEG评分、血清ox-LDL/HDL比值、hs-CRP/Alb比值、FAR均高于预后良好组（P<0.05）。多发脑梗死病灶、高NHISS评分、高aEEG评分、高ox-LDL/HDL比值、高hs-CRP/Alb比值和高FAR是ACI患者预后不良的危险因素（P<0.05）。aEEG评分、血清ox-LDL/HDL比值、hs-CRP/Alb比值、FAR预测ACI患者预后的曲线下面积分别为0.793、0.759、0.777、0.821，联合预测的曲线下面积为0.915，高于单独指标预测。结论：ACI预后不良患者aEEG评分、ox-LDL/HDL比值、hs-CRP/Alb比值、FAR均升高，上述指标联合应用预测ACI患者预后不良具有较高的价值。

关键词：振幅整合脑电图 ox-LDL/HDL比值 hs-CRP/Alb比值 FAR 急性脑梗死预后

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三维荧光结合区域积分鉴别酸枣仁及其掺伪品

山东中医药大学学报 2024年第4期48卷 485-493页

作者：刘亚丽王晓莉张嘉铖吕绪桢李健李峰张凤杰冯帅山东中医药大学药学院山东济南250355 聊城市人民医院药学部山东聊城252004 山东中医药大学附属医院心病科山东济南250014 山东中医药大学附属医院药学部山东济南250014 山东省高校中药质量控制与全产业链建设协同创新中心山东济南250355

目的:研究建立酸枣仁三维荧光光谱鉴别方法,为酸枣仁真伪鉴别提供依据。方法:建立6批不同产地酸枣仁三维荧光光谱,并利用三维荧光技术对2批酸枣仁伪品、3批掺伪品鉴别分析,采用荧光区域积分法对不同产地酸枣仁、酸枣仁伪品及掺伪品三维... 详细信息

目的:研究建立酸枣仁三维荧光光谱鉴别方法,为酸枣仁真伪鉴别提供依据。方法:建立6批不同产地酸枣仁三维荧光光谱,并利用三维荧光技术对2批酸枣仁伪品、3批掺伪品鉴别分析,采用荧光区域积分法对不同产地酸枣仁、酸枣仁伪品及掺伪品三维荧光图谱进行区域积分,将区域积分值进行相似度评价、聚类分析和主成分分析。结果:相似度结果显示,不同产地酸枣仁三维荧光图谱相似性均为1,相似性极高;聚类结果与主成分分析结果一致,明确将样品分为3类,均能通过区域积分值实现酸枣仁正品、伪品、掺伪品的区分。结论:利用三维荧光技术可以对不同产地酸枣仁质量进行一致性评价,实现酸枣仁真伪的快速鉴别,为酸枣仁的质量评价提供参考。

关键词：酸枣仁真伪鉴别三维荧光光谱区域积分相似性分析聚类分析主成分分析质量评价

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骆驼布鲁氏菌活疫苗A19株免疫试验

动物医学进展 2024年第5期45卷 137-139页

作者：高静周峰彭程胥元鹏王子坚聂龙祁张建春热孜婉南金鱼陈学生王新强甘肃省动物疫病预防控制中心甘肃兰州730046 阿克塞县畜牧兽医技术服务中心甘肃阿克塞736400 酒泉市畜牧兽医总站甘肃酒泉735000

为通过骆驼布鲁氏菌A19株疫苗免疫试验筛选出适宜的免疫方案,选择布鲁氏菌抗体检测阴性骆驼60头,按照不同剂量、不同年龄分4组颈部皮下注射免疫,并于免疫后不同时间采样检测免疫抗体。结果显示,1~3岁骆驼标准剂量(6×10^(10)CFU/头)免疫... 详细信息

为通过骆驼布鲁氏菌A19株疫苗免疫试验筛选出适宜的免疫方案,选择布鲁氏菌抗体检测阴性骆驼60头,按照不同剂量、不同年龄分4组颈部皮下注射免疫,并于免疫后不同时间采样检测免疫抗体。结果显示,1~3岁骆驼标准剂量(6×10^(10)CFU/头)免疫后23 d,免疫抗体阳性率为100%,至免疫12个月,免疫抗体阳性率0;1~3岁骆驼减低剂量(1×10^(9)CFU/头)免疫23 d,免疫抗体阳性率为94.1%,至免疫6个月,免疫抗体阳性率为0。3~12月龄骆驼标准剂量免疫38 d,免疫抗体阳性率为50%,免疫6个月,免疫抗体阳性率0。3~12月龄骆驼减低剂量免疫38 d,免疫抗体阳性率为16.7%,免疫6个月,免疫抗体阳性率为0。结果表明,1~3岁骆驼使用布氏菌活疫苗(A19株)标准剂量(6×10^(10)CFU/头)皮下注射免疫,效果最好。

关键词：骆驼布鲁氏菌活疫苗A19株免疫试验

Recent Advances in Fibrous Materials for Interfacial Solar Steam Generation

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Advanced Fiber Materials 2023年第3期5卷 791-818页

作者： Can Ge Duo Xu Heng Du Ze Chen Jingyu Chen Zhuoer shen Weilin Xu Qian zhang jian Fang College of Textile and Clothing Engineering Soochow UniversitySuzhou 215123China State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430200China

The scarcity of fresh water resources has become a serious issue hindering the sustainable development of modern civilization.The interfacial solar steam generation(ISSG)system that produces heat on material surface through photothermal conversion for desalination has been demonstrated as a promising candidate for practical application.Fibrous materials with unique flexibility,durability,processability,practicability,and multifunctionality have attracted considerable attention in the ISSG field.In this review,the basics of fibrous materials,such as their classification,manufacturing methods and flexible fibrous structure,are firstly introduced.Afterward,the outstanding properties of fibrous materials on different dimensions are demonstrated,as well as the versatile morphologies and structures that allow fibrous materials to carry out different roles in ISSG.Moreover,the practicability and multifunctionality of fibrous materials are illustrated in detail by combining specific cases to show their promising potential in practical ISSG application.Finally,existing challenges and future opportunities of fibrous material-based ISSG systems are discussed.

关键词： Fibrous material Interfacial solar steam generation Desalination Photothermal conversion

急性缺血性脑卒中CT生成MRI算法——基于影像组学的边缘感知扩散GAN

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计算机技术与发展 2024年第3期34卷 170-176页

作者：张美美秦品乐柴锐曾建潮翟双姣中北大学计算机科学与技术学院山西太原030051

相较于CT,急性缺血性脑卒中病灶在MRI上可清晰显示。鉴于患者中存在一些不适用于MRI检查的情况,如体内金属或幽闭恐惧症等,容易耽误患者病情。通过结合影像组学和扩散生成对抗网络(DiffusionGAN),该文提出了基于影像组学的边缘感知扩散G... 详细信息

相较于CT,急性缺血性脑卒中病灶在MRI上可清晰显示。鉴于患者中存在一些不适用于MRI检查的情况,如体内金属或幽闭恐惧症等,容易耽误患者病情。通过结合影像组学和扩散生成对抗网络(DiffusionGAN),该文提出了基于影像组学的边缘感知扩散GAN,实现了急性缺血性脑卒中患者的CT生成MRI。该算法弥补了现有CT生成MRI中病灶信息不足和边缘模糊的问题。具体而言,通过在CT上应用影像组学定位病灶,借助特征图辅助MRI生成,并提出边缘感知扩散GAN以提升生成MRI的边缘感知。实验证实,生成的MRI在峰值信噪比(PSNR)方面达到69.607,结构相似性(SSIM)为0.821,皮尔逊相关系数(PCC)为0.974,显著优于现有模型。医生评估显示生成的MRI不存在错误病灶,阳性/阴性分类准确度高达87.91%,表明这一创新性算法为解决在MRI不适用的特殊情况下的脑卒中诊断问题提供了新的途径。

关键词：医学图像生成计算机断层扫描(CT) 核磁共振成像(MRI) 急性缺血性脑卒中 DiffusionGAN 影像组学