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Proteomic analysis and miRNA profiling of human testicular endothelial cell-derived exosomes:the potential effects on spermatogenesis

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Asian Journal of Andrology 2022年第5期24卷 478-486页

作者： Wen-Peng song Sheng-Ji Gu Xiao-Hui Tan Yang-Yang Gu Wei-Dong song jian-Yu Zeng Zhong-Cheng Xin rui-Li Guan Department of Urology Peking University First HospitalBeijing 100034China Institute of Urology Peking UniversityBeijing 100034China Beijing Key Laboratory of Urogenital Diseases(Male)Molecular Diagnosis and Treatment Center Beijing 100034China Department of Dental Implant Center Beijing Stomatological HospitalSchool of StomatologyCapital Medical UniversityBeijing 100050China Department of Radiation Medicine Institute of Systems BiomedicineSchool of Basic Medical SciencesPeking University Health Science CenterBeijing 100191China

Testicular endothelial cells have been found to play an important role in spermatogenesis and fertility,but their mechanism is *** released by various cells are recognized as cell–cell communication mediators during the initiation and progression of many ***,the current study aimed to investigate the protein and miRNA components of human testicular endothelial cell-derived exosomes(HTEC-Exos)and to explore their potential effects on *** this study,HTEC-Exos were first isolated by the ultracentrifugation method,and then identified by nanoparticle tracking analysis,transmission electron microscopy(TEM),and western *** characteristics of HTEC-Exos were examined by liquid chromatography–mass spectrometry and microRNA(miRNA)chip *** analysis was performed to explore the potential role of the exosomal content on spermatogenesis.A total of 945 proteins were identified,11 of which were closely related to spermatogenesis.A total of 2578 miRNAs were *** them,30 miRNAs demonstrated potential associations with male reproductive disorders,such as azoospermia,and spermatogenesis *** particular,11 out of these 30 miRNAs have been proven to be involved in spermatogenesis based on available *** study provides a global view of the proteins and miRNAs from HTEC-Exos,suggesting that HTEC-Exos may function as potential effectors during the process of spermatogenesis.

关键词： exosomes microRNAs proteomics testicular endothelial cells

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Imaging of reflector in the earth with wavelet transform

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中国有色金属学会会刊：英文版 1994年第3期4卷 1-4,8页

作者： song, Shougen He, Jishen rui, Jiagau Central South Univ of Technology Changsha China

ＩＭＡＧＩＮＧＯＦＲＥＦＬＥＣＴＯＲＩＮＴＨＥＥＡＲＴＨＷＩＴＨＷＡＶＥＬＥＴＴＲＡＮＳＦＯＲＭＳｏｎｇ，Ｓｈｏｕｇｅｎ；Ｈｅ，Ｊｉｓｈｅｎ；Ｒｕｉ，Ｊｉａｇａｕ（ＤｅｐａｒｔｍｅｎｔｏｆＧａｄｇｏｇｙ，Ｃｅｎｔｒａ... 详细信息

ＩＭＡＧＩＮＧＯＦＲＥＦＬＥＣＴＯＲＩＮＴＨＥＥＡＲＴＨＷＩＴＨＷＡＶＥＬＥＴＴＲＡＮＳＦＯＲＭＳｏｎｇ，Ｓｈｏｕｇｅｎ；Ｈｅ，Ｊｉｓｈｅｎ；Ｒｕｉ，Ｊｉａｇａｕ（ＤｅｐａｒｔｍｅｎｔｏｆＧａｄｇｏｇｙ，ＣｅｎｔｒａｌＳｏｕｔｈＵｎｉｖｅｒｓｉｔｙｏ...

关键词： inversion imaging wavelet

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Identification and Characterization of a Novel Single Domain Antibody Against Ebola Virus

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Virologica Sinica 2021年第6期36卷 1600-1610页

作者： rui Wang Haiwei Zhang Cheng Peng jian Shi Huajun Zhang rui Gong CAS Key Laboratory of Special Pathogens and Biosafety Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan430071China University of Chinese Academy of Sciences Beijing100049China

Ebola virus(EBOV) belongs to the Filoviridae family and causes severe illnesses such as hemorrhagic fever with a high mortality rate up to 90%. Now two antibody drugs termed Inmazeb and Ebanga have been approved for treating EBOV infection. However, clinical studies have demonstrated that the mortality rate of the patients who received these two antibody drugs remains above 30%. Therefore, novel therapeutics with better efficacy is still desired. The isolated human IgG1 constant domain 2(CH2 domain) has been proposed as a scaffold for the development of C-based single domain antibodies(C-sd Abs) as therapeutic candidates against viral infections and other diseases. Here, we screened and identified a novel C-sd Ab termed M24 that targets EBOV glycoprotein(GP) from a C-sd Ab phage display library. M24 neutralizes the pseudotype EBOV with IC;of 0.8 nmol/L(12 ng/mL) and has modest neutralizing activity against authentic *** determination, including molecular docking and site mutation analysis, discloses that M24 binds to the internal fusion loop(IFL) within GP2, a transmembrane subunit of GP. Interestingly, we found that the binding of M24 to GP at pH5.5 has dramatically decreased compared to the binding at pH 7.5, which may lead to weak efficacy in the neutralization of authentic EBOV. Since no sd Ab against EBOV infection has been reported to date, our results not only give a proof of concept that sd Abs could be utilized for the development of potential therapeutic candidates against EBOV infection, but also provide useful information for the discovery and improvement of anti-EBOV agents.

关键词： Ebola virus(EBOV) Constant domain 2(CH2 domain) C-based single domain antibody(C-sdAb) Neutralize Fusion loop

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Supercritical superprocesses: Proper normalization and non-degenerate strong limit

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Science China Mathematics 2019年第8期62卷 1519-1552页

作者： Yan-Xia Ren Renming song rui Zhang LMAM School of Mathematical Sciences & Center for Statistical Science Peking University Beijing 100871 China Department of Mathematics University of Illinois Urbana IL 61801 USA School of Mathematical Sciences Nankai University Tianjin 300071 China School of Mathematical Sciences Capital Normal University Beijing 100048 China

Suppose that X ={Xt, t≥0;Pμ} is a supercritical superprocess in a locally compact separable metric space E. Let φ0 be a positive eigenfunction corresponding to the first eigenvalue λ0 of the generator of the mean semigroup of X. Then Mt := e-λ0t〈φ0,Xt〉 is a positive martingale. Let M∞ be the limit of Mt. It is known(see Liu et al.(2009)) that M∞ is non-degenerate if and only if the L log L condition is satisfied. In this paper we are mainly interested in the case when the L log L condition is not satisfied. We prove that, under some conditions, there exist a positive function γt on [0,∞) and a non-degenerate random variable W such that for any finite nonzero Borel measure μ on E,lim/t→∞γt〈φ0,Xt〉=W, a.s.-Pμ.We also give the almost sure limit of γt〈f, Xt〉for a class of general test functions f.

关键词： superprocesses Seneta-Heyde norming non-degenerate strong limit martingales

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Data-collection system for high-throughput X-ray absorption fine structure measurements

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Nuclear Science and Techniques 2016年第4期27卷 38-45页

作者： rui-You Lu Qian Gao song-Qi Gu Ya-Yun Yang rui Si Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201204 China University of Chinese Academy of Sciences Beijing 100049 China

A new X-ray absorption fine structure(XAFS)data-collection system based on the Experimental Physics and Industrial Control System software environment has been established at the BL14W1 beamline of the Shanghai Synchrotron Radiation Facility. The system provides for automatic sequential analysis of multiple samples for continuous high-throughput(HT) measurements. Specifically, 8 sample pellets are loaded into an alumina holder,and a high-precision two-dimensional translation stage is programmed to switch these samples automatically for collecting the XAFS spectrum of each sample in *** implement HT measurements via a graphical user interface developed with Control System ***, the successful operation of the HT XAFS system is demonstrated by running experiments on two groups of copper–ceria catalysts, each of which contains 8 different powder samples.

关键词： X射线吸收精细结构数据采集系统结构测量高通量上海同步辐射装置 XAFS谱测量与控制系统粉末样品

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New hybrid model of proton exchange membrane fuel cell

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2007年第5期8卷 741-747页

作者： WANG rui-min CAO Guang-yi ZHU Xin-jian Institute of Fuel Cell Department of Automation Shanghai Jiao Tong University Shanghai 200240 China

Model and simulation are good tools for design optimization of fuel cell systems. This paper proposes a new hybrid model of proton exchange membrane fuel cell (PEMFC). The hybrid model includes physical component and black-box com-ponent. The physical component represents the well-known part of PEMFC, while artificial neural network (ANN) component estimates the poorly known part of PEMFC. The ANN model can compensate the performance of the physical model. This hybrid model is implemented on Matlab/Simulink software. The hybrid model shows better accuracy than that of the physical model and ANN model. Simulation results suggest that the hybrid model can be used as a suitable and accurate model for PEMFC.

关键词： Proton exchange membrane fuel cell (PEMFC) Artificial neural network (ANN) Hybrid model Physical model

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Circular dichroism of graphene oxide： the chiral structure model

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Frontiers of Materials Science 2013年第1期7卷 83-90页

作者： Jing CAO Hua-Jie YIN rui song College of Chemistry and Chemical Engineering University of Chinese Academy of Sciences Beijing 100049 China Laboratory of Nanomaterials National Center for Nanoscience and Technology Beijing 100190 China

We have observed the circular dichroism signal of dilute graphene oxide (GO), then systematically investigated the chirality of GO and established a probable chiral unit model. This study may open up a new field for u... 详细信息

关键词： graphene oxide (GO) circular dichroism XPS chiral model

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Evaluation of Chaotic Demodulator for EBPSK Signals

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Journal of Electronic Science and Technology 2016年第3期14卷 262-267页

作者： song Zhang Guo-Sheng rui Yang Zhang the Department of Electronics and Information Engineering NavalAeronautical and Astronautical UniversityYantai 264001China

Based on the theory of Duffing oscillator weak signal detection and the technology of extended binary phase shift keying （EBPSK） modulation, the chaotic demodulator using the Duffing oscillator for EBPSK signals was proposed. The proposed demodulator could avoid the problem of demodulation filters design, and shows the excellent anti-noise capability of chaotic oscillator detection. Numerical and experimental tests were taken to investigate the impact of modulation parameters T and 0 on bit error performance of the proposed method, and the performance limits were gotten. The results show that the proposed chaotic demodulator works well under a very low signal-to-noise ratio （SNR） conditions, and gets SNR gains about 20 dB to 30 dB from the impulse filter.

关键词： Index Terms Duffing oscillator demodulation extended binary phase shift keying (EBPSK) receiver filter.

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A true triaxial strength criterion for rocks by gene expression programming

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Journal of Rock Mechanics and Geotechnical Engineering 2023年第10期15卷 2508-2520页

作者： jian Zhou rui Zhang Yingui Qiu Manoj Khandelwal School of Resources and Safety Engineering Central South UniversityChangsha410083China Institute of Innovation Science and SustainabilityFederation University AustraliaBallaratVIC3350Australia

Rock strength is a crucial factor to consider when designing and constructing underground *** study utilizes a gene expression programming(GEP)algorithm-based model to predict the true triaxial strength of rocks,taking into account the influence of rock genesis on their mechanical behavior during the model building process.A true triaxial strength criterion based on the GEP model for igneous,metamorphic and magmatic rocks was obtained by training the model using collected *** to the modified Weibols-Cook criterion,the modified Mohr-Coulomb criterion,and the modified Lade criterion,the strength criterion based on the GEP model exhibits superior prediction accuracy *** strength criterion based on the GEP model has better performance in R2,RMSE and MAPE for the data set used in this ***,the strength criterion based on the GEP model shows greater stability in predicting the true triaxial strength of rocks across different *** to the existing strength criterion based on the genetic programming(GP)model,the proposed criterion based on GEP model achieves more accurate predictions of the variation of true triaxial strength(s1)with intermediate principal stress(s2).Finally,based on the Sobol sensitivity analysis technique,the effects of the parameters of the three obtained strength criteria on the true triaxial strength of the rock are *** general,the proposed strength criterion exhibits superior performance in terms of both accuracy and stability of prediction results.

关键词： Gene expression programming(GEP) True triaxial strength Rock failure criteria Intermediate principal stress

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Surface reaction of Bacillus cereus biomass and its biosorption for lead and copper ions

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Journal of Environmental Sciences 2007年第4期19卷 403-408页

作者： PAN jian-hua LIU rui-xia TANG Hong-xiao State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beijing 100085 China.

In this study, the surface chemical functional groups of Bacillus cereus biomass were identified by Fourier transform infrared （FTIR） analytical technique. It had been shown that the B. cereus cells mainly contained carboxyl, hydroxyl, phosphate, amino and amide functional groups. The potentiometric titration was conducted to explain the surface acid-base properties of aqueous B. cereus biomass. The computer program FITEQL 4.0 was used to perform the model calculations. The optimization results indicated that three sitesthree pKas model, which assumed the cell surface to have three distinct types of surface organic functional groups based on the IR analysis results, simulated the experimental results very well. Moreover, batch adsorption experiments were performed to investigate biosorption behavior of Cu（Ⅱ） and Pb（Ⅱ） ions onto the biomass. Obviously, the adsorption equilibrium data for the two ions were reasonably described by typical Langmuir isotherm.

关键词： Bacillus cereus biosorption Fourier transform infrared (FTIR) acid-base characteristic heavy metals

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