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Controlling neuronal activity using light

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Controlling neuronal activity using light

Improved optrode array design and neural interface for optogenetics

中国神经科学学会第十届全国学术会议

作者： Li-Ping WANG Yi LU jian-qing pan Peng-Fei WEI Shenzhen Key Lab of Neuropsychiatric Modulation Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

Optogenetics enables rapid bi-directional control neural circuits on the milliseconds timescale and genetically targeted cell types by light *** the functional optical neural interface（ONI） in intact animals,we can study the function of specific neural circuits with behavioral output in animals from rodents to nonhuman *** this presentation,I will introduce the optogenetic platform we have established in our lab,including:optrode design and fabrication,an optimal biocompatibility modification method for building a low impedance and tissue friendly optrode/neural interface,optical stimulation devices,multi-channel recording and neural circuits modulation using light in freely moving *** another part of this presentation,I will also introduce a homemade signal processing toolkit for several important optical/electrophysiology usages,such as:reducing the optical stimulation artifacts on local-field potentials,identification the specify cell-type of neurons in extracellular multi-units activity by optical stimulation evoked firing patterns and analyzing the neural synchronization under the optical *** are providing the opening resource and research platform for brain mapping studies.

关键词： optogenetics neural circuits brain activity mapping

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Improved optrode array design and neural interface for optog...

Expression of Channelrhodopsin-2（ChR2） in serotonergic neurons using microRNA-regulated vector designs

中国神经科学学会第十届全国学术会议

作者： Yi LU Cheng ZHONG jian-qing pan Peng-Fei WEI Li-Ping WANG Shenzhen Key Lab of Neuropsychiatric Modulation Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

Objective Optogenetics is a technology that combines optical control and genetic targeting using cell-type specific and optically-sensitive proteins for the precise manipulation of neuronal *** technology has been successfully used to understand the mechanisms of neuropsychiatric diseases through the precise spatial and temporal control of specific groups of neurons in a neural ***,it remains a great challenge to integrate optogenetic modulation with electrophysiological and behavioral read out methods as a means to explore the causal,temporally precise,and behaviorally relevant interactions of neurons in the specific circuits of freely behaving ***,the aim of this study is to develop a novel optrode array for long term applications in awaken *** We have designed and fabricated a high spatial resolution,multi-channel,and drivable optrode array for optical stimulating and recording in different brain regions of a rodent model at one ***,we have also developed optimal modification methods to build a low impedance and tissue friendly optrode/neural interface based on electro-active and bio-active materials,such as conducting polymers,hydrogels,neurotrophins and *** According to the requirements in the application of implantable optrode arrays,evaluation processes and characterization of the optrode arrays,both in vitro and in vitro,were exploited and *** investigation includes the electrochemical properties and biocompatibility of the optrode/tissue interface,and the long-term stability of the electrophisiological recording signals in freely-behaving ***,some other devices based on the technique of optogenetics and electrophysiology were developed,which were used to investigate the complex neural circuit processing mechanisms at the functional *** This work will provide theoretic and technical support for the development of a new generation of optrode arrays,a

关键词： optogenetics optorde neural circuits neural modulation brain activity mapping

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Expression of Channelrhodopsin-2（ChR2） in serotonergic neu...

Soil Phosphorus Dynamics as Influenced by Land Use Changes in Humid Tropical, Southwest China

中国神经科学学会第十届全国学术会议

作者： jian-qing pan Cheng ZHONG Xue-Mei LIU Xiao-Lei LIU Li-Ping WANG Shenzhen Key Lab of Neuropsychiatric Modulation Shenzhen Institutes of Advanced TechnologyChinese Academy of Sciences

Objective The brain expresses a wide variety of microRNA（miRNAs）,some of which show cell type ***448 is located in the fourth intron of Htr2c and Htr2c receptors,which are expressed on the membrane of GABAergic neurons in Raphe ***,miR448 is a specific microRNA in GABAergic *** order to deliver light-sensitive genes into the serotonergic neurons within inbred rodent strains,such as BTBR T+tf/J,we developed a microRNA-regulated vector *** To drive the expression of the transgene exclusively in serotonergic neurons we constructed the synapsin promoter combined with 4x miR448 target sites *** insertion of 4xmiR448 target sequence at the 3’ end of a light-sensitive protein（ChR2,Arch,etc） of interest led to its post-transcriptional silencing in GABAergic neurons and not in serotonergic neurons because of the GABAergic-specific expression of the *** We observed high levels of ChR2-YFP expression in serotonergic neurons but not in GABAergic *** We have generated a new optogenetic vector,which is both highly specific for 5HT neurons and sufficiently powerful.

关键词： optogenetics serotonin microRNA

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Pedosphere 2005年第1期15卷 24-32页

作者： YANGJing-Cheng HUANGjian-Hui panqing-Min TANGjian-Wei HANXing-Guo LaboratoryofQuantitativeVegetationEcology InstituteofBotanyChineseAcademyofSciencesBeijing1000g3China LaboratoryofQuantitativeVegetationEcology InstituteofBotanyChineseAcademyofSciencesBeijing1000g3China／／BeijingNaturalHistoryMuseumBeijing100050China XishuangbannaTropicalBotanicalGarden ChineseAcademyofSciencesYunnan666303China

Land use changes can greatly influence soil phosphorus (P) dynamics, especially when converting native forests to agricultural land. Soils in Xishuangbanna, which is one of southwest China s tropical areas that maintain fragments of primary forests, were studied to a) evaluate the effect of two common land use changes, conversion of forests to agricultural land or rubber tree plantation, on the dynamics of available P and total P in bulk soils as well as total P in particle size fractions; b) assess the rel…

关键词： BRAZILIAN AMAZON ORGANIC-MATTER NUTRIENT POOLS DEFORESTATION FRACTIONS PHOSPHATE BIOMASS LOSSES FIRE

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基于RLS算法的过热汽温闭环辨识方法研究

华东电力 2009年第9期37卷 1595-1599页

作者：常太华江清潘朱红路华北电力大学自动化系北京102206

在火电机组运行过程中,出于系统运行稳定性与安全性的考虑,很难在开环条件下进行过热汽温对象模型辨识。而在闭环条件下,由于不可测噪声通过反馈环节与控制输入信号相关,用常规开环辨识算法进行建模将产生较大的估计误差。为克服闭环辨... 详细信息

在火电机组运行过程中,出于系统运行稳定性与安全性的考虑,很难在开环条件下进行过热汽温对象模型辨识。而在闭环条件下,由于不可测噪声通过反馈环节与控制输入信号相关,用常规开环辨识算法进行建模将产生较大的估计误差。为克服闭环辨识中噪声的影响,提出了基于RLS(recursive least squares,递归最小二乘)算法的两阶段闭环辨识方法。该方法将闭环辨识问题转换成两个开环环节进行信号处理,通过RLS滤波器的噪声消除构造出无噪声污染的中间信号,再将其应用于RLS滤波器进行模型辨识,达到精确建模的目的。将该方法应用于过热汽温对象的闭环辨识,仿真结果表明该方法建模精度高,能达到满意的辨识效果。

关键词：过热汽温 RLS算法闭环辨识模型辨识

Unlocking the future:Mitochondrial genes and neural networks in predicting ovarian cancer prognosis and immunotherapy response

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World Journal of Clinical Oncology 2025年第1期16卷 43-52页

作者： Zhi-jian Tang Yuan-Ming pan Wei Li Rui-Qiong Ma jian-Liu Wang Department of Obstetrics and Gynecology Peking University People’s HospitalBeijing 100044China Cancer Research Center Beijing Chest HospitalBeijing 101149China Department of Thoracic Surgery Sichuan Provincial People's HospitalChengdu 610072Sichuan ProvinceChina

BACKGROUND Mitochondrial genes are involved in tumor metabolism in ovarian cancer(OC)and affect immune cell infiltration and treatment *** To predict prognosis and immunotherapy response in patients diagnosed with OC using mitochondrial genes and neural *** Prognosis,immunotherapy efficacy,and next-generation sequencing data of patients with OC were downloaded from The Cancer Genome Atlas and Gene Expression *** genes were sourced from the MitoCarta3.0 *** discovery cohort for model construction was created from 70% of the patients,whereas the remaining 30% constituted the validation *** the expression of mitochondrial genes as the predictor variable and based on neural network algorithm,the overall survival time and immunotherapy efficacy(complete or partial response)of patients were *** In total,375 patients with OC were included to construct the prognostic model,and 26 patients were included to construct the immune efficacy *** average area under the receiver operating characteristic curve of the prognostic model was 0.7268[95% confidence interval(CI):0.7258-0.7278]in the discovery cohort and 0.6475(95%CI:0.6466-0.6484)in the validation *** average area under the receiver operating characteristic curve of the immunotherapy efficacy model was 0.9444(95%CI:0.8333-1.0000)in the discovery cohort and 0.9167(95%CI:0.6667-1.0000)in the validation *** The application of mitochondrial genes and neural networks has the potential to predict prognosis and immunotherapy response in patients with OC,providing valuable insights into personalized treatment strategies.

关键词： Ovarian cancer Mitochondria Prognosis Immunotherapy Neural network

雷公藤内酯醇抑制小鼠巨噬细胞血管内皮生长因子的表达

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生殖与避孕 2006年第3期26卷 142-145页

作者：潘建青曾叶杨业金程静欧阳曙明华中科技大学同济医学基础医学院生物化学与分子生物学系武汉430030

目的:探讨雷公藤内酯醇(TP)对小鼠巨噬细胞表达血管内皮生长因子(VEGF)的抑制作用。方法:以脂多糖(LPS)激活的小鼠腹腔巨噬细胞为研究对象,利用RT-PCR、免疫组化检测不同剂量雷公藤对激活巨噬细胞VEGFmRNA表达及VEGF合成的影响。结果:T... 详细信息

目的:探讨雷公藤内酯醇(TP)对小鼠巨噬细胞表达血管内皮生长因子(VEGF)的抑制作用。方法:以脂多糖(LPS)激活的小鼠腹腔巨噬细胞为研究对象,利用RT-PCR、免疫组化检测不同剂量雷公藤对激活巨噬细胞VEGFmRNA表达及VEGF合成的影响。结果:TP可以抑制LPS诱导的巨噬细胞VEGFmRNA表达及VEGF合成,并呈剂量依赖性。结论:TP抑制巨噬细胞VEGFmRNA转录及VEGF合成可能是其治疗子宫内膜异位症的作用机理之一。

关键词：雷公藤内酯醇(TP) 小鼠巨噬细胞血管内皮生长因子(VEGF)

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胃部CT检查的体位研究

实用放射学杂志 2002年第3期18卷 188-190页

作者：鲍健魏建芬潘青华翟晓冬李威无锡市第五人民医院CT室无锡市第五人民医院CT室江苏无锡214073

目的　本文主要研究了不同的检查体位在胃部CT扫描中的作用。方法　通过 30例正常成人在四种不同体位上的扫描 ,分别研究分析了各胃区显示状况与体位改变的关系 ,进一步分析了体位改变对各胃区CT图像的影响。结果　本研究结果表明 ,通... 详细信息

目的　本文主要研究了不同的检查体位在胃部CT扫描中的作用。方法　通过 30例正常成人在四种不同体位上的扫描 ,分别研究分析了各胃区显示状况与体位改变的关系 ,进一步分析了体位改变对各胃区CT图像的影响。结果　本研究结果表明 ,通过对检查体位的调节 ,有利于CT对胃壁及胃周器官的显示。胃底贲门区的最佳显示体位为仰卧头低足高位 ;胃体区的最佳显示体位为俯卧位 ;胃窦区的最佳显示体位为右侧卧位。分别与仰卧位比较 ,均有显著差异 (Ρ <0 .0 5 )。结论　仰卧位与各胃区的最佳显示体位结合 ,行双体位CT扫描 ,有助于提高胃部CT检查质量。

关键词：胃体位断层摄影,X线计算机

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土壤有机质对农田管理措施的动态响应

生态学报 2003年第4期23卷 787-796页

作者：杨景成韩兴国黄建辉潘庆民中国科学院植物研究所植被数量生态学开放研究实验室北京100093

土壤有机质在农田肥力、环境保护、农业可持续发展等方面具有重要意义。它不仅决定农作物产量 ,而且在全球碳素循环中起着重要作用。由于大气 CO2 浓度升高与全球气候变暖等一系列环境问题的加剧 ,全球碳素循环受到越来越多的关注。农... 详细信息

土壤有机质在农田肥力、环境保护、农业可持续发展等方面具有重要意义。它不仅决定农作物产量 ,而且在全球碳素循环中起着重要作用。由于大气 CO2 浓度升高与全球气候变暖等一系列环境问题的加剧 ,全球碳素循环受到越来越多的关注。农田具有大气 CO2 源和库的双重潜力。历史上由于人类对农田的过度开垦和耕种 ,造成土壤有机质含量大幅度下降 ,降低了农田的作物产量潜力 ;同时导致大量的碳以 CO2 形式由陆地生态系统排放到大气圈 ,加剧了全球温室效应。大量研究结果表明 ,诸如耕作、种植制度、施肥等农田管理措施能够显著地影响土壤有机质动态 ,而免耕、提高复种指数、合理的轮作换茬、有机肥料和化肥的施用以及弃耕农田还林还草等保护性管理措施则能够提高农田土壤有机质含量 ,使农田起到大气 CO2汇的作用。综述了近年来农田管理对土壤有机质动态影响研究方面的进展。

关键词：农田土壤碳土壤有机质耕作种植制度轮作施肥