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Interference of Y-27632 on the signal transduction of transforming growth factor beta type 1 in ocular Tenon capsule fibroblasts

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International Journal of Ophthalmology(English edition) 2012年第5期5卷 576-581页

作者： Xiao-Hui Zhang, jian-ming wang Department of Ophthalmology the Second Affiliated Hospital College of Medicine Xi'an Jiaotong University Xi'an 710004 Shaanxi Province China Department of Pharmacology Xijing Hospital Fourth Military Medical University Xi'an 710032 Shaanxi Province China

AIM: To investigate the interfering effect of Y-27632, a ROCK-I selective inhibitor, on the signal transduction pathway of transforming growth factor-beta 1 (TGF-beta 1) in ocular Tenon capsule fibroblasts (OTFS) in vitro. METHODS: After OTFS from passages 4 to 6 47 vitro were induced by TGF-beta 1 and then treated by Y-27632, the changes of the OTFS cell cycles were analyzed via flow cytometry, and the proteins expression of the alpha -smooth muscular actin (alpha -SMA), connective tissue growth factor (CTGF), collagen I were calculated by Western blot. After OTFS treated by the different concentrations of Y-27632, the expression levels of the alpha -SMA, CTGF and collagen I mRNA were assayed by RT-PCR. RESULTS: Y-27632 had no markedly effect on the OTFS cell cycles. After treated by TGF-beta 1, OTFS in G1 period significantly increased. The cell cycles distribution by both TGF-beta 1 and Y-27632 had no remarkable difference from that in control group. Y-27632 significantly inhibited the proteins expressions of both alpha -SMA and CTGF, while to some extent inhibited that of collagen I. TGF-beta 1 significantly promoted the proteins expressions of alpha -SMA, CTGF and collagen I. After OTFS treated by both TGF-beta 1 and Y-27632, of alpha -SMA, the protein expression was similar with that in control group (P=0.066>0.05), but the protein expression of CTGF or collagen I, respectively, was significantly different from that in control group (P=0.000<0.01). The differences of expressions of the alpha -SMA, CTGF and collagen I mRNA in 30, 150, 750 mu mol/L Y-27632 group were statistically significant, compared with those in control group, respectively (alpha -SMA, P=0.002, 0.000, 0.000; CTGF, P=0.014, 0.002, 0.001; collagen I,P=0.003, 0.002, 0.000). CONCLUSION: Blocking the Rho/ROCK signaling pathway by using of Y-27632 could inhibit the cellular proliferation and the expression of both CTGF and alpha -SMA whatever OTFS induced by TGF-beta 1 or not. Y-27632 suppressed t

关键词： Y-27632 ocular Tenon's capsule fibroblasts transforming growth factor beta type 1 α-smooth muscular actin connective tissue growth factor collagen I

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Identification of methylation profile of HOX genes in extrahepatic cholangiocarcinoma

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World Journal of Gastroenterology 2011年第29期17卷 3407-3419页

作者： Yi Shu Bing wang Ji wang jian-ming wang Sheng-Quan Zou General Surgery Department of Tongji Hospital Tongji Medical College of Huazhong University of Science and Technology1095 Jiefang AvenueWuhan 430030Hubei ProvinceChina

AIM:To identify methylation profile and novel tumor marker of extrahepatic cholangiocarcinoma(CCA)with high throughout ***:Differential methylation profile was compared between normal bile duct epithelial cell lines and CCA cell lines by methyl-DNA immunoprecipitation (MeDIP)***-polymerase chain reaction (BSP)was performed to identify the methylated allels of target *** of target genes was investigated before and after the treatment with DNA demethylating *** of candidate genes was also evaluated by immunofluorescence in 30 specimens of CCA tissues and 9 normal bile duct ***:Methylation profile of CCA was identified with MeDIP microarray in the respects of different gene functions and signaling ***,97 genes with hypermethylated CpG islands in the promoter region were homeobox *** top 5 hypermethylated homeobox genes validated by BSP were HOXA2(94.29%),HOXA5(95.38%),HOXA11 (91.67%),HOXB4(90.56%)and HOXD13(94.38%).Expression of these genes was reactivated with 5’-aza2’-*** expression differences were found between normal bile duct and extrahepatic CCA tissues(66.67%-100%vs 3.33%-10%).CONCLUSION:HOXA2,HOXA5,HOXA11,HOXB4 and HOXD13 may work as differential epigenetic biomarkers between malignant and benign biliary tissues.

关键词： DNA methylation Epigenetic Promoter microarray Cholangiocarcinoma

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Anti-proliferative effect of olmesartan on Tenon's capsule fibroblasts

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International Journal of Ophthalmology(English edition) 2016年第5期9卷 669-676页

作者： Xuan wang Ya-Zhi Fan Liang Yao jian-ming wang Department of Ophthalmology the Second Affiliated Hospital of Xi'an Jiaotong University

AIM： To evaluate the inhibitive effect of olmesartan to fibroblast proliferation and the anti-scarring effect in Tenon＇s capsule, both in vitro and in vivo.· METHODS： Human primary Tenon＇s capsule fibroblasts were cultured in vitro, treated with up titrating concentrations of olmesartan. The rate of inhibition was tested with methyl thiazol tetrazolium（MTT） ***-time PCR was performed to analyze changes in m RNA expressions of the fibrosis-related factors： matrix metalloproteinase-2（MMP-2）, tissue inhibitor of metalloproteinase（TIMP-1,2） and proliferating cell nuclear antigen（PCNA）. Thirty rabbits were divided into5 groups（3, 7, 14, 21, and 28d）. A rabbit conjunctiva flap model was created in each eye. Olmesartan solution was injected subconjunctivally and then evaluated its anti-proliferation and anti-fibrosis effects through the histological morphology and immunohistochemistry of MMP-2 and PCNA in each group. Only the 7d group was treated with Masson＇s trichrome to compare the neovascularization in the subconjunctiva area.·RESULTS： In vitro, cultured Tenon＇s capsule human fibroblasts showed a dose dependent inhibition by olmesartan in MTT. Olmesartan reduced m RNA expressions of MMP-2 and PCNA but increased m RNA expressions of TIMP-1 and TIMP-2. In vivo, the rabbit eyes treated with olmesartan at 3rd, 7th, 14 thand 21stdays demonstrated a significant reduced expressions of MMP-2 and PCNA compared with control eye, no significant difference observed in 28 thday group. The cellular proliferation and neovascularization was suppressed by olmesartan in Masson＇s trichrome observation.·CONCLUSION： By inhibiting fibroblasts in vitro and in vivo, olmesartan prevents the proliferation and activity of fibroblasts in scar tissue formation, which might benefit glaucoma filtering surgery.

关键词： olmesartan trabeculectomy anti-proliferative matrix metalloproteinase-2 proliferating cell nuclear antigen

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Delayed cardiac tamponade after simultaneous transcatheter atrial septal defect closure and left atrial appendage closure device implantation: a particular case report

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Journal of Geriatric Cardiology 2019年第12期16卷 898-901页

作者： jian-ming wang Qi-Guang wang Xian-Yang ZHU Department of Congenital Heart Disease General Hospital of Northern Theater Command

Percutaneous left atrial appendage(LAA)occlusion evolved as an alternative treatment to the patients who are contraindicated or cannot tolerate oral anticoagulants with nonvalvular atrial fibrillation(AF)at risk of stroke or systemic embolism.[1]Abnormal hemodynamic changes in elder atrial septal defect(ASD)patients cause remodeling of the left atrium,which eventually leads to right heart failure.[2]As the ASDs elderly are associated with a higher incidence of AF,simultaneous transcatheter ASD and LAA closure has become a new effective therapeutic ***,only a limited number of articles involving cardiac tamponade complications have been published in the ***’s more,previous studies involving early hemodynamically irrelevant pericardial effusion after the procedure attribute to multiple repositioning attempts of LAA occluder or delivery sheath injured the atrial wall.

关键词： Atrial fibrillation Atrial septal defect Cardiac catheterization Cardiac tamponade Left atrial appendage

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High temperature oxidation resistance of CrN/(Ti,Al,Zr,Cr)N bilayer films deposited by multi-arc ion plating

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Journal of Iron and Steel Research International 2017年第3期24卷 343-349页

作者： Shi-lu Zhao Zhen Zhang Jun Zhang jian-ming wang Zheng-gui Zhang Shuang-hong wang School of Mechanical Engineering Shenyang University Shenyang 110044 Liaoning China Paint Shop Planning BMW Brilliance Automotive Ltd. Shenyang 110113 Liaoning China

CrN/（Ti,Al,Zr,Cr） N bilayer films were successfully deposited on cemented carbide（ WC-8% Co） substrates by multi-arc ion plating process using two Ti-Al-Zr alloy targets and one pure Cr target. As a result of bilayered structure and addition of alloying elements（ e. g. Al and Cr）,the films exhibited excellent high temperature oxidation resistance under both short-term isothermal（ up to 800 ℃） and long-term cyclic（ up to 600 ℃） exposure conditions. Combined with pre-established outstanding tribological properties（ e. g. maximum hardness of 4 000HV0. 01 and maximum adhesion strength over 200 N）,these observations make such films quite a promising candidate to extend the cutting tool life span and boost the performance in high-productivity,high-speed and high-feed cutting or in dry machining conditions.

关键词： Nitride Bilayer film Cemented carbide Multi-arc ion plating High temperature oxidation resistance

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Simultaneous interventional therapy for coarctation of the aorta combined with intracristal ventricular septal defect in older age adult

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Journal of Geriatric Cardiology 2022年第6期19卷 481-484页

作者： jian-ming wang Xian-Yang ZHU Jia-wang XIAO Qi-Guang wang Department of Congenital Heart Disease General Hospital of Northern Theater CommandShenyangChina

Coarctation of the aorta(CoA)is an uncommon congenital cardiovascular malformation,which is rare associated with several other cardiac and vascular anomalies,such as bicuspid aortic valve,ventricular septal defect(VSD),patent ductus arteriosus and aortic arch hypoplasia.[1,2]Open surgical repair is the usual treatment for neonatal CoA,whereas balloon angioplasty and stent placement are the treatment of choice for older children and adults.[3]

关键词： ventricular septal balloon

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Effect of targeted magnetic nanoparticles containing 5-FU on expression of bcl-2, bax and caspase 3 in nude mice with transplanted human liver cancer

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World Journal of Gastroenterology 2007年第23期13卷 3171-3175页

作者： jian-ming wang Bao-Lai Xiao jian-Wei Zheng Hai-Bing Chen Sheng-Quan Zou Department of General Surgery of Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430 030 Hubei Province China Department of Material Wuhan University of Technology Wuhan 430030 Hubei Province China

AIM: To investigate the anti-tumor effect and mechanisms of magnetic nanoparticles targeting hepatocellular carcinoma. METHODS: Human hepatocellular carcinoma was induced in nude mice, and the mice were randomly divided into group A receiving normal saline, group B receiving magnetic nanoparticles containing 5-fluorouracil (5-FU), group C receiving 5-FU, and group D receiving magnetic nanoparticles containing 5-FU with a magnetic field built in tumor tissues. The tumor volume was measured on the day before treatment and 1, 4, 7, 10 and 13 d after treatment. Tumor tissues were isolated for examination of the expression of bcl-2, bax and caspase 3 by immunohistochemical method, reverse transcription polymerase chain reaction and Western blotting. RESULTS: The tumor volume was markedly lower in groups C and D than in groups A and B (group C or D vs group A or B, P < 0.01). The volume was markedly lower in group D than in group C (P < 0.05). The expression of protein and mRNA of bcl-2 was markedly lower in groups C and D than in groups A and B (group C or D vs group A or B, P < 0.01), and was markedly lower in group D than in group C (P < 0.01). The expression of bax and caspase 3 in groups C and D was signif icantly increased, compared with that in groups A and B (P < 0.01). CONCLUSION: The targeted magnetic nanoparticles containing 5-FU can improve the chemotherapeutic effect of 5-FU against hepatocellular carcinoma by decreasing the expression of bcl-2 gene, and increasing the expression of bax and caspase 3 genes.

关键词： Hepatocellular carcinoma Magnetic nanoparticles Fluorouracil Bcl-2 Bax Caspase 3

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Treatment for hemoptysis after percutaneous closure of a large intralobar pulmonary sequestration in elderly patient

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Journal of Geriatric Cardiology 2020年第9期17卷 585-588页

作者： jian-ming wang Qi-Guang wang Duan-Zhen ZHANG Xian-Yang ZHU Department of Congenital Heart Disease General Hospital of Northern Theater CommandShenyangChina

Pulmonary sequestration is a rare congenital cardiovascular malformation.[1,2]Open surgical excision is the usual treatment,although thorascopic intervention and transcatheter embolization have been ***,only a limited number of articles involving percutaneous closure the rare pulmonary malformations have been published in the literature.[3]What’s more,previous studies involving percutaneous pulmonary sequestration closure with repeated respiratory symptoms at the very young age suggested that the optimal therapeutic strategy has yet to be determined.[4]We hereby describe a particular case of percutaneous closure of a large intralobar pulmonary sequestration at elderly age.

关键词： Cardiac catheterization Hemoptysis Interventional embolization Pulmonary sequestration

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Assessment of cardiac function in infants with transposition of the great arteries after surgery: comparison of two methods

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World Journal of Pediatrics 2018年第4期14卷 373-377页

作者： Fan Yang Roberta Iacobelli jian-ming wang Fiore Salvatore Iorio Cardiac Surgery Department Hebei Province Children's HospitalNo.133Jianhua South StreetShijiazhuang 050031China Pediatric Cardiology Center of Ospedale Pediatrico Bambino Gesù Piazza S.Onofrio 400165 RomeItaly

Background Assessment of cardiac function is crucial in pediatric patients undergoing cardiovascular surgery, monitoring cardiac output and changing hemodynamic conditions during surgery accordingly is important to improve post-surgical outcome. We aimed to measure cardiac index (CI) and maximal rate of the increase of left ventricular pressure dp/dt(max) with the pressure recording analytic method (PRAM, MostCare?) and compared it with transthoracic echocardiographic cardiac index estimation in infants with transposition of the great arteries (TGA) undergoing surgical correction. Methods We enrolled 74 infants with TGA consecutively into this study. CI and dp/dt(max) were measured with PRAM and echocardiography at 0, 4, 8, 12, 24 and 48 h postoperatively. Blood brain natriuretic peptide (BNP) and blood lactate (Lac) were measured at baseline and after operation. Results The median age at surgery was 13 days (range 1–25 days) with an average weight of 3.24 kg (range 2.31–4.17 kg). CI esti-mated by PRAM was 1.11 ± 0.12 L/min/m2 (range 0.69–1.36) and by Doppler echocardiography was 1.13 ± 0.13 L/min/m2 (range 0.76–1.40). dp/dt(max) estimated by PRAM was 1.31 ± 0.03 mmHg/s (range 1.23–1.43) and by Doppler echocardiography was 1.31 ± 0.04 L/min/m2 (range 1.25–1.47). CI (r = 0.817,P< 0.001) and dp/dt(max) (r = 0.794,P< 0.001) measured by two meth-ods were highly correlated with a linear relation. Blood BNP and lactate increased to the highest level at 8–12 h post-operatively. Conclusions In the early post-operative period, PRAM provides reliable estimates of cardiac index and dp/dt(max) value compared with echocardiographic measurements. PRAM through mostcare? is a reliable continuous monitoring method for peri-operative management in children with congenital heart disease.

关键词： Cardiac index Doppler echocardiography dp/dt(max) Pressure recording analytic method Transposition of the great arteries

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Influence of nano-scaled dispersed second phase on substructure of deformed dispersion strengthened copper alloy

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Journal of Central South University of Technology 2005年第Z1期12卷 50-53页

作者： CHENG jian-yi wang ming-pu CAO jian-guo ZHAO Xue-long GUO ming-xing School of Materials Science and Engineering Jiujiang University Jiujiang 332005 China School of Metallurgy Science and Engineering Central South University Changsha 410083 China Hailiang Group Co. Ltd Zhuji 311835 China School of Materials Science and Engineering Central South University Changsha 410083 China Hailiang Group Co. Ltd Zhuji 311835 China Hailiang Group Co. Ltd Zhuji 311835 China School of Materials Science and Engineering Central South University Changsha 410083 China

The deformation behavior of dispersion strengthened copper alloy Cu-Al2O3 was studied by TEM. The results show that nano-scaled dispersed second phase not only increases dislocation density in matrix, but also has an important influence on the dislocation substructure. The presence of fine dispersed Al2 O3 particles results in a uniform and random dislocation distribution in matrix copper and causes the difficulty in formation of dislocation cell structure and the decrease in the amount of cell structure during deformation. Deformation gives rise to much more dislocations and dislocation cells form more difficultly and the decrease in the cell size with the increase of dispersion degree.

关键词： dispersed phase particle dispersion strengthened copper alloy deformation substructure

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