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Production of chitosan scaffolds by lyophilization or electrospinning: which is better for peripheral nerve regeneration?

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Neural Regeneration Research 2021年第6期16卷 1093-1098页

作者： Yu-Xuan Wu Hao Ma jian-lan wang Wei Qu Department of Hand Microsurgery First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina Department of Plastic and Reconstructive Surgery Shanghai Ninth People’s HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghaiChina Department of Traditional Chinese Medicine First Affiliated Hospital of Dalian Medical UniversityDalianLiaoning ProvinceChina

Both lyophilization and electrospinning are commonly used to make chitosan ***,it remains unknown which method is better for cell *** this study,we established the following groups:(1)lyophilization group-chitosan scaffolds were prepared by lyophilization method and seeded with Schwann cells from Sprague-Dawley rats aged 3-5 days;(2)electrospinning group-chitosan scaffolds were prepared by electrospinning method and seeded with Schwann cells;(3)control group-Schwann cells were cultured on culture *** growth of Schwann cells was evaluated by immunofluorescence and scanning electron *** blot assay was performed to explore the mechanism of Schwann cell *** materials were non-toxic and suitable for the growth of Schwann *** pores produced by electrospinning were much smaller than those produced by *** proliferation rate and adhesion rate of Schwann cells in the electrospinning group were higher than those in the lyophilization *** cells cultured on electrospinning scaffolds formed a Bungner band-like structure,and a much greater amount of brain-derived neurotrophic factor was secreted,which can promote the growth of *** findings show that the chitosan scaffold prepared by the electrospinning method has a nanofiber structure that provides an extracellular matrix that is more favorable for cell-cell *** electrospinning method is more suitable for nerve regeneration than the lyophilization *** research was approved by the Medical Ethical Committee of Dalian Medical University(approval ***1-2016-045)on March 3,2016.

关键词： cells factor growth in vitro model peripheral nerve plasticity protein rat regeneration

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Variations of SST and Thermocline Depth in the Tropical Indian Ocean During Indian Ocean Dipole Events

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Journal of Ocean University of China 2010年第2期9卷 129-134页

作者： SUN Shuangwen lan jian wang Yi Physical Oceanography Laboratory Ocean University of China Qingdao 266100 P. R. China

Interannual variations in the surface and subsurface tropical Indian Ocean were studied using HadlSST and SODA datasets. Wind and heat flux datasets were used to discuss the mechanisms for these variations. Our results indicate that the surface and subsurface variations of the tropical Indian Ocean during Indian Ocean Dipole （IOD） events are significantly different. A prominent characteristic of the eastern pole is the SSTA rebound after a cooling process, which does not take place at the subsurface layer. In the western pole, the surface anomalies last longer than the subsurface anomalies. The subsurface anomalies are strongly correlated with ENSO, while the relationship between the surface anomalies and ENSO is much weaker. And the subsurface anomalies of the two poles are negatively correlated while they are positively correlated at the surface layer. The wind and surface heat flux analysis suggests that the thermocline depth variations are mainly determined by wind stress fields, while the heat flux effect is important on SST.

关键词： Indian Ocean Dipole ENSO thermocline depth SST

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Snowpack variations and their hazardous effects under climate warming in the central Tianshan Mountains

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Advances in Climate Change Research 2024年第3期15卷 442-451页

作者： jian-Sheng HAO Yan wang lan-Hai LI Key Laboratory of land Surface Pattern and Simulation Institute of Geographic Sciences and Natural Resources ResearchChinese Academy of Sciences(CAS)Beijing 100101China Xinjiang Key Laboratory of Water Cycle and Utilization in Arid Zone Urumqi 830011China State Key Laboratory of Desert and Oasis Ecology Xinjiang Institute of Ecology and GeographyChinese Academy of SciencesUrumqi 830011China Tianshan Snowcover and Avalanche Observation and Research Station of Xinjiang Xinyuan 835800China CAS Research Center for Ecology and Environment of Central Asia Urumqi 830011China

Climate change alters snowpack evolution,which in turn influences the likelihood of snow avalanches and flood *** lack of systemic observational data on key snow characteristics in high mountains remains a scientific challenge in terms of systematically elucidating the dynamic chain of variations in climate-snowpack-snow *** restricts our understanding and poses challenges in the prediction of snow-related disaster *** such,this study analysed the variations of temperature and snowfall and the physical characteristics of snowpacks based on ground-based observations from the Kunse River Valley situated in the Tianshan Mountains from 1967 to *** results reveal that the temperature increased significantly by 0.32°C per decade(plands,road transportation,water-electricity infrastructure and several other human ***,these insights are critical for providing vital information that can deepen our understanding of the impact of climate change on snowpack characteristics and improve management strategies for snow-related disaster prevention and mitigation.

关键词： Climate change Snowpack variations Snow-related disaster Tianshan Mountains

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Study of 3-D Numerical Simulation for Gas Transfer in the Goaf of the Coal Mining

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Journal of China University of Mining and Technology 2007年第2期17卷 152-157页

作者： WU Zheng-yan jianG Shu-guang HE Xin-jian wang lan-yun LIN Bai-quan School of Mining and Safety Engineering China University of Mining ＆ Technology Xuzhou Jiangsu 221008 China

In order to simulate field distribution rules,mathematical models for 3-D air flows and gas transfer in the goaf of the coal mining are established,based on theories of permeability and dynamic dispersion through porous media. A gas dispersion equation in a 3-D field is calculated by use of numerical method on a weighted upstream multi-element balance. Based on data of an example with a U type ventilation mode,surface charts of air pressure distribution and gas concentration are drawn by Graphtool software. Finally,a comparison between actually measured results in the model test and the numerical simulation results is made to proves the numerical implementation feasible.

关键词： 3D numerical simulation of gas transfer in the goaf air pressure distribution in the goaf weighted upstream multi-element balance numerical simulation method

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Microfacies and diagenetic alteration in a semi-deep to deep lacustrine shale: The Yanchang Formation in the Ordos Basin, China

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Petroleum Science 2024年第3期21卷 1524-1538页

作者： Bin Bai Jing-Yue Hao Ling Fu Yu-Xi Liu jian-Peng wang lan wang Kevin GTaylor Lin Ma PetroChina Research Institute of Petroleum Exploration and Development Beijing100083China Department of Earth and Environmental Sciences University of ManchesterM139PLUK College of Earth Sciences Jilin UniversityChangchun130061jilinChina Department of Chemical Engineering University of ManchesterM139PLUK

The mineralogical development and diagenetic sequence of lacustrine shales in the Chang 7 Member of the Yanchang Formation in the Ordos Basin are detailed studied.A model of their depositional system and a diagenetic diagram are proposed in this *** detailed petrographic,mineralogical,and elemental analyses,four distinct shale types are identified:argillaceous shale,siliceous shale,calcareous shale,and carbonate,clay,and silt-bearing *** main diagenetic process in argillaceous shale is the transformation of illite to smectite,negatively impacting shale *** shale undergoes carbonate cementation and quartz dissolution,contributing to increased porosity,particularly in *** shale experiences diagenesis characterised by carbonate formation and dissolution,with a prevalence of *** carbonate,clay,and silt-bearing shale,the dissolution of K-feldspar contributes to illitization of *** shale,characterised by more clay minerals and lower mesopore volume,is identified as a potential hydrocarbon *** shale,with the highest pore volume and abundant inter-mineral pores,emerges as a promising shale oil *** findings contribute to a comprehensive understanding of shale properties,aiding in the prediction of shale oil exploration potential in the studied area.

关键词： Ordos Basin Yanchang Formation Chang 7 Member Diagenesis Microfacies

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A direct electrochemical biosensor for rapid glucose detection based on nitrogen-doped carbon nanocages

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Rare Metals 2024年第5期43卷 2184-2192页

作者： lan-lan Li Yu Zhao Li-Jia Pan jian-Bin Xu Yi Shi College of Mechanical and Electrical Engineering Henan Agricultural UniversityZhengzhou 450002China School of Electronic Science and Engineering Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing210093China Department of Electronic Engineering The Chinese University of HongKongHongkong 999077China

Given the increasing number of diabetic patients,rapid and accurate detection of glucose in body fluids is *** study developed a direct electrochemical biosensor for glucose based on nitrogen-doped carbon nanocages(NCNCs).NCNCs possess a large specific surface area of 1395 m^(2)·g^(-1),a high N atomic content of 9.37%and good biocompatibility,which is favorable for enzyme loading and electron *** surface average concentration of electroactive glucose oxidase on NCNCs was 2.82×10^(-10)mol·cm^(-2).The NCNC-based direct electrochemical biosensor exhibited a high sensitivity of 13.7μA·(mmol·L^(-1))^(-1)·cm^(-2),rapid response time of 5 s and an impressive electron-transferrate constant(ks)of 1.87 s^(-1).Furthermore,we investigated an NCNC-based direct electron transfer(DET)biosensor for sweat glucose detection,which demonstrated tremendous promise for non-invasive wearable diabetes diagnosis.

关键词： Direct electron transfer(DET) Nitrogen-doped carbon nanocages(NCNCs) Glucose oxidase Biosensor

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Safety and efficacy of omalizumab for antihistamine-resistant chronic urticaria in children:a case series and literature review

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World Journal of Pediatrics 2024年第3期20卷 294-298页

作者： Wei-Fang Zhu Chen wang jian-Jun Qiao lan-Juan Li Department of Dermatology First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China State Key Laboratory for Diagnosis and Treatment of Infectious Diseases National Clinical Research Center for Infectious DiseasesCollaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310003China

Chronic urticaria(CU)is a common skin disease characterized by the occurrence of wheals,with or without angioedema,lasting more than 6 *** prevalence of CU is about 0.7%[1],but higher in children,with an incidence of 1.38%[1].The occurrence of severe pruritus and unpredictable wheals may severely impact patients'quality of life,cause psychological problems,including sleep disorders and depression,and lead to economic burden due to limitation on daily life and work[2,3].

关键词： edema prevalence children

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Molecularly imprinted sensor based on carboxylated carbon nanotubes/Keggin-type polyoxometalates nanocomposite for the detection of furazolidone

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Tungsten 2024年第2期6卷 394-409页

作者： Yu-lan Huang Bing Zhang Yu-Lian Li Li wang Le Dong jian Li College of Ocean Food and Biological Engineering Jimei UniversityXiamen 361021China College of Oceanology and Food Science Quanzhou Normal UniversityQuanzhou 362000China

Due to its properties of mutagenic,teratogenic,and carcinogenic,the detection of furazolidone(FZD)in aquaculture is of great importance for food safety and human *** this study,molecularly imprinted fi lms modifi ed with carboxylated multi-walled carbon nanotube-phosphomolybdic acid composite were used to fabricate an electrochemical sensor for the determination of *** nanocomposites were characterized using infrared spectroscopy,scanning electron microscopy,energy-dispersive X-ray spectroscopy,and X-ray diff *** electrochemical characteristics of the modifi ed electrodes were examined using electrochemical impedance spectroscopy,cyclic voltammetry,and diff erential pulse *** sensor exhibited exceptional catalytic *** calibration curves were acquired in the concentration range of 6 nmol·L^(−1)to 0.6μmol·L^(−1),with a limit of detection of 3.38 nmol·L^(−1).Additionally,the sensor proved successful in recognizing FZD in shrimp samples with satisfactory recoveries and *** method provides a strategy to construct a molecularly imprinted electrochemical sensing platform using nanomaterials,which has great promise in the field of food safety.

关键词： Phosphomolybdate Furazolidone Polyoxometalates Carboxylated multi-walled carbon nanotube Molecularly imprinted polymers Electrochemical sensor

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Comparing the efficacy and safety of low,medium,and high dosages of selexipag for treating pulmonary hypertension:A systematic review and meta-analysis

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Animal Models and Experimental Medicine 2024年第1期7卷 56-70页

作者： Shang wang Yi Yan jian Zhang Ping Yuan Ci-Jun Luo Hong-Ling Qiu Hui-Ting Li jian Xu lan wang Tian-lan Li Rong jiang Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Heart Center and Shanghai Institute of Pediatric Congenital Heart Disease Shanghai Children's Medical CenterNational Children's Medical CenterShanghai Jiaotong University School of MedicineShanghaiChina Department of Respiratory and Critical Care Medicine The 416 Hospital of Nuclear IndustryThe Second Affiliated Hospital of Chengdu Medical CollegeChengduChina Department of Hematology The Affiliated Hospital of Qingdao UniversityQingdaoChina

Background:The maintenance dosage of selexipag is categorized as low,medium or *** order to assess the efficacy and safety of different dosages of selexipag for the risk stratification of pulmonary arterial hypertension(PAH),we performed a sys-tematic review and ***:Studies assessing PAH risk stratification indices,such as the World Health Organization functional class(WHO-FC),six-minute walk distance(6MWD),N-terminal pro-B-type natriuretic peptide(NT-proBNP)level,right atrial pressure(RAP),cardiac index(CI)and mixed venous oxygen saturation(SvO2),were ***:Thirteen studies were *** led to improvements in the 6MWD(MD:24.20 m,95%CI:10.74-37.67),NT-proBNP(SMD:-0.41,95%CI:-0.79-0.04),CI(MD:0.47 L/min/m^(2),95%CI:0.17-0.77)and WHO-FC(OR:0.564,95%CI:0.457-0.697).Subgroup analysis demonstrated that all three dosages improved the 6MWD.A moderate dosage led to improvements in the CI(MD:0.30 L/min/m^(2),95%CI:0.15-0.46)and WHO-FC(OR:0.589,95%CI:0.376-0.922).Within 6 months of treatment,only the WHO-FC and CI were significantly improved(OR:0.614,95%CI:0.380-0.993;MD:0.30 L/min/m^(2),95%CI:0.16-0.45,respectively).More than 6 months of treatment significantly improved the 6MWD,WHO-FC and NT-proBNP(MD:40.87 m,95%CI:10.97-70.77;OR:0.557,95%CI:0.440-0.705;SMD:-0.61,95%CI:-1.17-0.05,respectively).Conclusions:Low,medium,and high dosages of selexipag all exhibited good *** treatment lasted for more than 6 months,selexipag exerted obvious effects,even in the low-dosage *** finding is important for guiding individualized treatments.

关键词： individualized treatments meta-analysis prostacyclin receptor agonist risk stratification systematic review

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Constructing highly safe and long-life calcium ion batteries based on hydratedvanadium oxide cathodes featuring a pillar structure

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Rare Metals 2024年第6期43卷 2597-2612页

作者： Chun-Fang wang Shi-Wei Zhang lan Huang Yuan-Min Zhu Feng Liu jian-Chuan wang Li-Ming Tan Chun-Yi Zhi Cui-Ping Han Powder Metallurgy Research Institute Central South UniversityChangsha410083China Songshan Lake Materials Laboratory Dongguan 523808China Research Institute of Interdisciplinary Science and School of Materials Science and Engineering Dongguan University of TechnologyDongguan 523808China Department of Materials Science and Engineering City University of Hong KongHong Kong 999077China Faculty of Materials Science and Energy Engineering/Institute of Technology for Carbon Neutrality Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China

Calcium-ion batteries(CIBs)have generated intense interest due to the growing demand for safer,cheaper,and large-scale energy storage ***,their development is still in its infancy,owing to the lack of suitable cathodes for sustaining reversiblc Ca^(2+)intercalation/***,layered H_(2)V_(3)O_(8)(HVO)with Zn^(2+)pre-insertion(ZHVO)is reported as a high-rate and highly durable cathode material for *** existence of Zn^(2+)and H_(2)O pillars could expand the interlayer spacing up to 1.8 nm,which is favorable for the diffusion of bulky Ca^(2+).The formation of Zn-O bonds facilitates electron transfer and enhances electrical ***,the ZHVO cathode achieves superior capacity performance(213.9 mAh·g^(-1)at 0.2 A·g^(-1))and long lifespan(78.3%for 1,000 cycles at 5 A·g^(-1))compared to pristine *** functional theory(DFT)calculations revealed that Zn^(2+)moved during Ca^(2+)intercalation,thereby reducing the diffusion energy barrier and facilitating Ca^(2+)***,a safe aqueous calcium ion cell was successfully assembled.

关键词： Multivalent batteries Calcium-ion batteries Layered structure Vanadium oxide Cathode materials

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