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Functional characterization of the promoter of carbonyl reductase 1 gene in porcine endometrial cells

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2017年第7期18卷 626-634页

作者： Ai-ling ZHANG Xian-yue SUN Qi YIN jian-hua zeng Zhe ZHANG Jia-qi LI Hao ZHANG Guangdong Development Center of Applied Ecology and Ecological Engineering in Universities Biology and Food Engineering Institute Guangdong University of Education Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding National Engineering Research Center for Breeding Swine Industry National & Local Joint Engineering Research Center for Livestock and Poultry Breeding College of Animal Science South China Agricultural University

Prostaglandins （PGs） play a critical role in porcine reproduction, of which prostaglandin E2 （PGE2） and prostaglandin F2a （PGF2a） exert antiluteolytic and luteolysis actions, respectively. As a rate-limiting enzyme, carbonyl reductase 1 （CBR1） catalyzes the conversion of PGE2 to PGF2a. A high ratio of PGE2：PGF2a is beneficial to the establishment and maintenance of porcine pregnancy. PG is essential for the establishment of pregnancy which resembles the proinflammatory response and nuclear factor KB （NF-KB） is involved in the process. Bioinformatic analysis has shown that NF-KB is a possible factor bound to two cis-regulatory elements in CBRI promoter. In this study, we cloned the 2997 bp （-2875/＋122） of the promoter, and constructed six 5＇-deleted dual-luciferase reporter recombinant vectors. In endometrial cells, the region of P2 （-16401＋7） exhibited the greatest transcriptional activity at driving luciferase expression, but not significantly different from that of P1 （-2089/＋7）. The activity of P1, P2, and P3 （-1019/＋7） was highly significantly higher than that of others （P〈0.01）, suggesting that two positive regulatory elements were likely present in the regions of -1640/-1019 and -1019/-647. The results also showed that the -1640/ -647 region was indispensable for the promoter. The results of chromatin immunoprecipitation （CHIP） demonstrated that the NF-KB subunit p65 binds to one site around -15451-1531. Using four reference genes, we found that the over-expression of p65 enhanced the expression of CBR1 （P〈0.05） in porcine endometrial epithelial cells, while knockdown of the p65 did not down-regulate the CBRI expression. These results indicated that NF-KB （p65） could bind to the special element of CBR1 gene promoter in porcine endometrial epithelial cells in vitro. The binding site of NF-KB was a positive regulator for the CBR1 gene promoter, but was not necessary for the basic expression.

关键词： Pig Carbonyl reductase 1 (CBR1) Promoter NF-KB Endometrium

Electron paramagnetic resonance study on Cu[(C6H11)2PS2]2 complex single crystal

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Acta Chimica Sinica(English Edition) 1988年第4期6卷 302-307页

作者： HAN Shi-Ying~* SUI Yun-Xia JIN Tong-Zheng Center of Materials Analyses,Nanjing University,NanjingXU Zheng LIN jian-hua YOU Xiao-zeng Coordination Chemistry Institute,Nanjing University,Nanjing Center of Materials Analyses Nanjing University Nanjing Coordination Chemistry Institute Nanjing University Nanjing

X-band EPR studies on Cu[(C6H11O)2PS2]2 complex single crystal at room tempera-ture were *** EPR spectrum shows that the hyperfine structure is produced by 63Cu nuclei,and the ligand hyperfine structure by ■p *** spin-Hamiltonian parameters were rigorouslycalculated using a least-squares-fitting technique,specially adapted to noncoincident g and A *** principal values of g tensor imply that Cu2+ occupies the site consisting of four ligandsS,and is tetragonal symmetric,but the principal values of A tensor in Cu-S4 plane reveal a largeanisotropy caused by the presence of two ligands *** of principal axes for g and A tensors iscoincident,and they are perpendicular to the Cu-S4 *** was found that the interactionbetween electron spin and ligands 31p nuclear spin is isotropic and the hyperfine-coupling constantAP related ligands was obtained.

关键词： EPR C6H complex single crystal Electron paramagnetic resonance study on Cu PS

伊犁马体尺性状非遗传因素分析及遗传参数估计

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畜牧兽医学报 2017年第10期48卷 1855-1862页

作者：马玉辉高利李海肖建华曾亚琦东北农业大学动物医学学院哈尔滨150030 昭苏县畜牧兽医站昭苏835600 新疆农业大学动物科学学院乌鲁木齐830052

旨在探讨非遗传因素对伊犁马体尺性状的影响及各体尺性状的遗传参数,本研究利用新疆伊犁昭苏县2005-2014年年龄在1~11岁的伊犁马体尺性状的1 556条记录,对影响伊犁马体尺性状的非遗传因素进行最小二乘方差分析,并利用动物模型约束最大似... 详细信息

旨在探讨非遗传因素对伊犁马体尺性状的影响及各体尺性状的遗传参数,本研究利用新疆伊犁昭苏县2005-2014年年龄在1~11岁的伊犁马体尺性状的1 556条记录,对影响伊犁马体尺性状的非遗传因素进行最小二乘方差分析,并利用动物模型约束最大似然(Restricted maximum likelihood,REML)方法估计体尺性状的遗传力及性状之间的遗传相关。结果表明,场、年龄和出生年度效应对伊犁马体高、体长、胸围和管围性状均有极显著影响(P0.05)。体高、体长、胸围和管围的遗传力分别为0.87、0.67、0.40和0.52;体长与胸围的遗传相关最高,相关系数为0.93,体高与管围的表型相关最高,相关系数为0.65;体高与体长、胸围和管围分别呈现极显著表型相关(P<0.01),体长与胸围和管围分别呈现极显著表型相关(P<0.01),管围和胸围也呈现极显著表型相关(P<0.01)。上述研究为伊犁马今后在遗传评定与遗传参数估计中设计合理的统计分析模型提供了必要的参考依据。

关键词：伊犁马体尺性状非遗传因素遗传参数

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动态增强MR在节细胞神经瘤诊断中的应用

实用放射学杂志 2008年第1期24卷 58-61页

作者：周建军曾维新周康荣王建华曾蒙苏程伟中复旦大学附属中山医院上海市影像医学研究所复旦大学附属中山医院上海市影像医学研究所上海200032

目的分析节细胞神经瘤的MRI表现及其血液动力学特征,以提高其诊断准确性。方法经手术病理证实的节细胞神经瘤9例,分别经MRI自旋回波T1WI、快速自旋回波T2WI、屏气扰相梯度回波T1WI动态增强扫描。仔细复习MRI扫描结果并和手术病理作回顾... 详细信息

目的分析节细胞神经瘤的MRI表现及其血液动力学特征,以提高其诊断准确性。方法经手术病理证实的节细胞神经瘤9例,分别经MRI自旋回波T1WI、快速自旋回波T2WI、屏气扰相梯度回波T1WI动态增强扫描。仔细复习MRI扫描结果并和手术病理作回顾性对照分析。结果9例节细胞神经瘤中,脊柱旁6例(颈部2例,纵隔1例,腹膜后3例),肾上腺3例。肿瘤直径3.7~16.4 cm,平均7.1 cm。SE T1WI为均匀低信号4例,混杂低信号5例;FSE T2WI显著高信号肿瘤3例,高低信号混杂5例,等信号1例。SPGR动态增强动脉期轻微强化,门脉期和延迟期为轻度进行性延迟强化。强化方式包括线条交织状强化6例,漩涡状强化2例,斑点状强化1例。3例肿瘤内血管强化。所有肿瘤境界清楚,瘤体自身受压变形7例,形成伪足样尖角4例。5例肿瘤推移邻近血管,所有与肿瘤解剖关系密切的血管形态正常。结论节细胞神经瘤MRI征象具有特点,动态MR可提高诊断率。

关键词：节细胞神经瘤磁共振成像动态增强扫描

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内燃机专业实验室建设模式的研究与实践

邵阳学院学报（自然科学版） 2004年第3期1卷 77-79页

作者：杨建华曾东和邵阳学院湖南邵阳422004

介绍了内燃机专业实验室建设的新模式 ,即按照为“产品研究、开发、制造、检测、使用、维修、改进”工程链全过程服务的“工程模式” ,及其在实践中形成的集教学、科研与服务地方经济建设于一体的实验室多功能特色 .

关键词：内燃机专业实验室工程模式研究实践

Clinicopathological differences between patients with schistosomal appendicitis and non schistosomal appendicitis:A retrospectively study of past ten years

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World Journal of Clinical Cases 2025年第2期13卷 24-32页

作者： Xiao-Yi Wang Yao Hao Zi-jian Wang Xiu-Liang Xu jiang-hua Yang Department of Infectious Diseases The First Affiliated of Wannan Medical CollegeWuhu 241001Anhui ProvinceChina

BACKGROUND Chronic schistosomiasis causes multiple organ and multiple system diseases,especially the digestive *** eggs are mainly deposited in the stomach,liver and colorectal,but a few eggs are deposited in the appendix and cause *** present,there are few studies on schistosomal *** To explore the differences in epidemiological,clinical and pathological characteristics between schistosomal appendicitis and non-schistosomal appendicitis over the past decade in order to assess the impact of schistosomiasis on *** The differences of general data,clinical data and laboratory examination data of patients with appendicitis from October 2013 to October 2023 were retrospectively *** patients were divided into two groups for *** were 136 patients in schistosomal appendicitis group and 5418 patients in non-schistosomal appendicitis *** Schistosomal appendicitis accounted for 2.45%of all patients with appendicitis,and the annual proportion in the past decade was 2.2%,2.9%,1.8%,1.9%,3.4%,3.1%,1.9%,1.6%,3%,2.6%,*** prevalence of schistosomal appendicitis was middle-aged and elderly males,with an average age of 61.73±15.335 *** main population of non-schistosomal appendicitis was middle-aged men,with an average age of 35.8±24.013 years(P<0.001).The distribution of pathological types of appendicitis was different between the two groups(P<0.001).The incidence of acute suppurative appendicitis in non-schistosomal appendicitis was higher than that in schistosomal appendicitis[odds ratio(OR)=0.504;95%confidence interval(CI):0.349-0.728;P<0.001].The proportion of acute attack of chronic appendicitis in schistosomal appendicitis was higher than that in non-schistosomal appendicitis(OR=2.614;95%CI:1.815-3.763;P<0.001).The proportion of schistosomal appendicitis patients complicated with colorectal cancer was higher than that of nonschistosomal appendicitis patients(OR=5.087;95%CI:1.427-

关键词： Schistosomal appendicitis Schistosomiasis Appendix Colorectal cancer Clinicopathological characteristics

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肾上腺淋巴瘤的影像学表现

实用放射学杂志 2007年第10期23卷 1325-1327页

作者：周建军曾维新周康荣王建华曾蒙苏丁建国程伟中复旦大学附属中山医院放射科上海市影像医学研究所复旦大学附属中山医院放射科上海市影像医学研究所上海200032

目的探讨动态增强影像学对肾上腺淋巴瘤的诊断价值。方法回顾性分析5例经手术病理证实的肾上腺淋巴瘤病例,5例皆为B细胞型非何杰金氏淋巴瘤,术前经MDCT动态增强检查,其中2例经MR动态增强检查,MR检查序列为SET1WI、FSET2WI和SPGR平扫和... 详细信息

目的探讨动态增强影像学对肾上腺淋巴瘤的诊断价值。方法回顾性分析5例经手术病理证实的肾上腺淋巴瘤病例,5例皆为B细胞型非何杰金氏淋巴瘤,术前经MDCT动态增强检查,其中2例经MR动态增强检查,MR检查序列为SET1WI、FSET2WI和SPGR平扫和动态增强。所有病例影像资料均经3名高年资医师分析阅片,分别确定病变特征、浸润范围、软组织肿块的范围,信号特征,病变的强化程度等。结果5例肾上腺淋巴瘤8个病灶中,双侧3例,单侧2例;病灶呈椭圆形6个,三角形2个。肿瘤直径4.3～11.9cm,平均6.5cm。CT平扫肿瘤均匀密实,动态增强动脉期轻度强化,门脉期中度强化。MRSE-T1WI呈低信号,信号略不均匀,FSE-T2WI脂肪抑制呈等低或略高信号,低于其它肾上腺原发或继发恶性肿瘤,且信号不均匀,可见多发线条状高信号影。SPGR动态增强早期可见线条状强化,门脉期强化更明显,趋均匀。结论肾上腺淋巴瘤具有一定的影像学特点。

关键词：肾上腺淋巴瘤磁共振成像体层摄影术 X线计算机动态增强扫描

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城市污水管网地理信息系统设计

江苏环境科技 2004年第2期17卷 21-23页

作者：金建华曾德飞杨晓芳武汉理工大学湖北武汉430070

以地理信息系统(GIS)为技术支持,结合污水管网管理的实际需求,在VB环境下利用GeoMap控件进行二次开发,建立了污水管网地理信息系统。该系统充分发挥了GIS对空间、属性数据的编辑、管理、显示和查询的功能,并实现了在GIS环境下对污水管... 详细信息

以地理信息系统(GIS)为技术支持,结合污水管网管理的实际需求,在VB环境下利用GeoMap控件进行二次开发,建立了污水管网地理信息系统。该系统充分发挥了GIS对空间、属性数据的编辑、管理、显示和查询的功能,并实现了在GIS环境下对污水管网流量的计算和水质的分析功能。

关键词：城市污水管网地理信息系统 GIS 水质数据库

Nonmetallic Inclusion Control of 350 km/h High Speed Rail Steel

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Journal of Iron and Steel Research International 2009年第3期16卷 20-26,36页

作者： ZHAO Ke-wen zeng jian-hua WANG Xin-hua School of Metallurgical and Ecological Engineering University of Science and Technology Beijing Beijing100083 China Panzhihua Iron and Steel (Group) Co Panzhihua 617067 Sichuan China

Inclusion has an important effect on quality of high speed rail steel. In consideration of the lower acceptance percentage of the inclusion and its constraint against the requirement for large scale production of 350 km/h high speed rail steel in Panzhihua Iron and Steel （Group） Co,the technology of nonmetallic inclusion control for 350 km/h high speed rail steel was studied. An optimized model of the argon-blowing in ladle furnace （LF）,the control of the components of the ladle slag,and the technique of calcium treatment for the molten steel was brought forward. Using the researched technology,the removal ratio of the inclusion was increased and the components,distribution,and shape of the inclusion in the rail steel were changed,which resulted in a reduction in the average total oxygen content to 10.17×10^-6 and an increase in the comprehensive acceptance percentage of the nonmetallic inclusion from 48.21% to 98.1%. Test has shown that this metallurgical technology can meet the requirement for large scale production of 350 km/h high speed steel in Panzhihua Iron and Steel （Group） Co.

关键词： high Speed rail steel inclusion refining calcium treatment total oxygen content