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Current susceptibilities of brown planthopper Nilaparvata lugens to triflumezopyrim and other frequently used insecticides in China

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Insect Science 2021年第1期28卷 115-126页

作者： Xun liao Peng-Fei Xu Pei-Pan Gong Hu Wan jian-hong li nstitute of Crop Protection Guizhou UniversityGuiyangChina Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanChina

The brown planthopper is a notorious rice pest in many areas of *** evolution of insecticide resistance in Nilaparvata lugens has become a serious problem in the effective control of this pest in the paddy *** this article,the current susceptibility of *** field populations to novel mesoionic insecticide triflumezopyrim and major classes of chemical insecticides was determined and *** monitoring results indicated that field populations of *** had developed low resistance to triflumezopyrim(resistance ratio,RR:1.3-7.3-fold)during 2015-2018 in China,and the median lethal concentration values varied from 0.05 to 0.29 mg/***,during 2017 to 2018,field populations of *** showed high resistance levels to thiamethoxam(RR:456.1-1025.6-fold),imidacloprid(RR:2195.3-6899.0-fold)and buprofezin(RR:1241.57521.7-fold),moderate to high resistance levels to dinotefuran(RR:97.6-320.1-fold),clothianidin(RR:69.4-230.1-fold)and isoprocarb(RR:44.1-108.0-fold),and low to moderate levels of resistance to chlorpyrifos(RR:12.0-29.7-fold)and nitenpyram(RR:6.9-24.1-fold).In contrast,*** just showed low resistance to sulfoxaflor(RR:3.3-8.5-fold)and etofenprox(RR:5.0-9.1-fold)in the ***,the P450 gene CYP6ER1 was found to be significantly overexpressed in all five field populations of *** collected in 2018 when compared with a laboratory susceptible *** findings will provide useful information to delay the evolution of insecticide resistance in ***.

关键词： Nilaparvata lugens(Stal) P450 gene susceptibility triflumezopyrim

Structure and magnetic properties of nanocrystalline dysprosium powders

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Rare Metals 2020年第1期39卷 28-35页

作者： hong-jian li Qiong Wu Ming Yue Yu-Qing li Rong-Chun Zhu Jing-Ming liang Jiu-Xing Zhang College of Materials Science and Engineering Key Laboratory of Advanced Functional MaterialsMinistry of Education of ChinaBeijing University of TechnologyBeijing100124China

In this current study,nanocrystalline Dy powders were prepared by melt-spinning and subsequent highenergy *** effect of ball-milling time on the structure and magnetic properties of the powders was *** crystal structure and microstructure of the melt-spun ribbons and ball-milled powders were observed by X-ray diffraction(XRD),scanning electron microscopy(SEM)and transmission electron microscopy(TEM).Magnetic measurement of all samples was performed with vibrating sample magnetometer(VSM).XRD results indicate that the average crystal grain size of the powders decreases from 90.09 nm of the ribbons to 10.42 nm of the4-h ball-milled *** TEM observation shows that the grains are fine and *** Neel temperature(TN)decreases from 182 K of the ribbons to 172 K of the powders,while the Curie temperature(TC)increases from100 to 130 K,demonstrating that the grain size has substantial influence on the magnetic transition ***,at 60 K,as the ball-milling time increases,the coercivity of the powders increases first,peaking at 0.48 T for 2-h milling,then drops again,while the remanence of the powders decreases monotonic *** a result,the powders milled for 2 h exhibit an optimal maximum energy product of 64.0 kJ·m-3,demonstrating the good potential of these powders as a permanent magnet at low temperatures.

关键词： Dysprosium Nanocrystalline Low temperature Magnetic properties

A de novo transcriptomic analysis to reveal functional genes in Apolygus lucorum

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Insect Science 2016年第1期23卷 2-14页

作者： De-Pan Cao Yang liu Jin-Jin Wei jian-hong li Gui-Rong Wang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing Laboratory of Pesticide College of Plant Science & Technology Huazhong Agricultural University Wuhan China

The widespread planting of genetically engineered cotton producing the CrylAc toxin has led to significantly reduced pesticide applications since 1997. However, conse- quently, the number of green mirid bugs （GMB）, Apolygus lucorum （Meyer-Diir） has in- creased. So far the GMB, instead of the cotton bollworm Helicoverpa armigera （Hiibner）, has become the major pest in the transgenic Bt cotton field and has influenced cotton yield. Disproportionately, only a few studies on GMB at molecular level have been re- ported. libraries from both third instar nymphs and adults were sequenced using Illumina technology, producing more than 106 million short reads and assembled into 63 029 uni- genes of mean length 597 nt and N50 813 nt, ranging from 300 nt to 9771 nt. BLASTx analysis against Nr, Swissprot, GO and COG was performed to annotate these unigenes. As a result, 26 478 unigenes （42.01%） matched to known proteins and 107 immune-related, 320 digestive-related and 53 metamorphosis-related genes were detected in these annotated unigenes. Additionally, we profiled gene expression using mapping based differentially expressed genes （DEGs） strategy between the two developmental stages： nymph and adult. The results demonstrated that thousands of genes were significantly differentially ex- pressed at different developmental stages. The transcriptome and gene expression data provided comprehensive and global gene resources of GMB. This transcriptome would improve our understanding of the molecular mechanisms of various underlying biological characteristics, including development, digestion and immunity in GMB. Therefore, these findings could help elucidate the intrinsic factors of the GMB resurgence, offering novel pest management targets for future transgenic cotton breeding.

关键词： Apolygus lucorum digestion immunity metamorphosis transcriptome

Microwave absorption properties of Ag naowires/carbon black composites

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Chinese Physics B 2017年第2期26卷 349-356页

作者： Hai-Long Huang Hui Xia Zhi-Bo Guo Yu Chen hong-jian li School of Physics and Electronics Central South University Changsha 410083 China

The composite that can absorb the high-performance electromagnetic（EM） wave is constructed into a sandwiched structure composed of carbon black（CB）/ethylene-vinyl acetate（EVA） and Ag naowires（Ag NWs）. The Ag NWs sandwiched between two CB/EVA layers are used to improve the absorption properties of composite. The effects of EVA-to-CB weight ratio, concentration and diameter of Ag NWs with a thickness of 0.4 mm on microwave absorption are *** results indicate that for an EVA-to-CB weight ratio of 1：3, Ag NW concentration of 1.0 mg/100 m L, and average diameter of 56 nm, the reflection loss（RL） of the composite is below-10 d B in a frequency range of 9.3 Ghz–18.0 GHz, with the minimum values of-40.0 d B and-25.6 d B at 13.5 GHz and 15.3 GHz, respectively. A finite element method（FEM）is used for calculating the RL of the composite. The calculated results are in agreement with the experimental data.

关键词： microwave absorption sliver nanowires finite element method polymer-based composites

Isolation and Characterization of a Dichlorvos-Degrading Strain DDV-1 of Ochrobactrum sp.

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Pedosphere 2006年第1期16卷 64-71页

作者： ZHANG Xiao-Hua ZHANG Guo-Shun ZHANG Zhong-Hui XU jian-hong li Shun-Peng Key Lab of Microbiological Engineering of Agricultural Environment Ministry of Agriculture Nanjing Agricultural University Nanjing 210095 （China）. jiangsu-Polytechnic College of Agriculture and Forestry Jurong 212400 （China）

The objective of this research was to isolate a dichlorvos (2,2-dichlorovinyl dimethyl phosphate)-degrading strain of Ochrobactrum sp., and determine its effectiveness in remediation of a dichlorvos-contaminated soil. A dichlorvos-degrading bacterium (strain DDV-1) was successfully isolated and identified as an Ochrobactrumsp. based on its 16S rDNA sequence analysis. Strain DDV-1 was able to utilize dichlorvos as a sole carbon source, and the optimal pH and temperature for its cell growth and degradation were 7.0 and 30 ℃, respectively. Also, the growth and degradation of strain DDV-1 showed the same response to dissolved oxygen. In addition, the soil degradation test indicated that in soil spiked with 100 mg L-1 or 500 mg L-1 dichlorvos and inoculated with 0.5% or 1.0% (v/v) strain DDV-1, complete degradation of dichlorvos could be achieved in 24 h. The present study showed that strain DDV-1 was a fast dichlorvos-degrading bacterium in soil. However, further research will be needed to clarify the degradation pathway and the properties of the key enzymes involved in its biodegradation.

关键词： degradation dichlorvos 16S rDNA soil test strain DDV-1

Elevated Levels of Soluble ST2 were Associated with Rheumatoid Arthritis Disease Activity and Ameliorated Inflammation in Synovial Fibroblasts

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Chinese Medical Journal 2018年第3期131卷 316-322页

作者： lian-Jie Shi Chen liu jian-hong li Xiao-Yun Zhu Ying-Ni li jiang-Tao li Department of Rheumatology and Immunology Peking University International Hospital Beijing 102206 China Department of Rheumatology and Immunology Peking University People's Hospital and Beijing Key Laboratory for Rheumatism Mechanism and Immune Diagnosis(BZ0135) Beijing 100044 China State Key Laboratory of Natural and Biomimetic Drugs School of Pharmaceutical Sciences Peking University Beijing 100191 China Department of Orthopaedics Peking University International Hospital Beijing 102206 China Department of Nuclear Medicine Peking University International Hospital Beijing 102206 China Department of Pathology Peking University International Hospital Beijing 102206 China Department of Rheumatology and Immunology The First People's Hospital of Yibin Yibin Sichuan 644000 Chin

Background：Much evidence has demonstrated that interleukin （IL）-33 plays an important role in rheumatoid arthritis （RA）.However,there have been limited studies about soluble ST2,a receptor for 1L-33,in *** aims of this study were to detect the levels of ST2 in the serum and synovial fluid of RA patients and to reveal the association of these levels with disease activity and the function of ST2 in ***：A total of 56 RA patients and 38 age-matched healthy controls were enrolled in this *** fluid samples were collected from another 30 RA patients and 20 osteoartbritis *** and synovial fluid levels of ST2 were measured by *** addition,the levels of ST2 in the serum of RA patients before and after therapy were *** function of ST2 in RA was revealed by the results of an in vitro cell assay,where recombinant ST2 proteins were used to treat peripheral blood mononuclear cells （PBMCs） and RA synovial fibroblasts （RASFs）.Results：Serum-soluble ST2 levels were significantly higher in RA patients （127.14 ± 61.43 pg/ml） than those in healthy controls （78.37 ± 41.93 pg/ml,P 〈 0.01）.Synovial fluid-soluble ST2 levels （41.90 ± 33.58 pg/ml） were much higher in RA patients than those in osteoarthritis patients （19.71 ± 16.72 pg/ml,P 〈 0.05）.RA patients who received effective therapy for 6 months showed decreased serum-soluble ST2 levels （113.01 ± 53.90 pg/ml） compared to baseline （139.59 ± 68.36 pg/ml）（P =0.01）.RA patients with high disease activity had higher serum-soluble ST2 levels （162.02 ± 56.78 pg/ml） than those with low disease activity （94.67 ± 40.27 pg/ml,P =0.001）.Soluble ST2 did not affect IL-1β,IL-6,IL-8,or tumor necrosis factor-α （TNF-o） expression in PBMCs from RA ***,soluble ST2 ameliorated the expressions of IL-33 and IL-1 β but not that of IL-6,IL-8,or TNF-α in resting ***,in the RASFs stimulated by TNF-α plus IL-1 β,soluble ST2 showed extensive suppressive effects on the expression of IL-6,IL-8,

关键词： Inflammatory Cytokines Interleukin-33 Rheumatoid Arthritis Soluble ST2

Effect of V2O5 Supported on the Sulfated TiO2 for Low-temperature SCR of NO by NH3

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环境科学前沿（中英文版） 2014年第2期3卷 6-12页

作者： Ludi Zhang jian li hong He Wenjun liang Quanming liang Key Laboratory of Beijing on Regional Air Pollution Control Beijing university of Technology Beijing 100124 China

In Vitro Construction of Effective M1GS Ribozymes Targeting HCMV UL54 RNA Segments

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Acta Biochimica et Biophysica Sinica 2005年第3期37卷 210-214页

作者： Yun-Zhen SU hong-jian li Yue-Qin li Hao-Jun CHEN Dong-Sheng TANG Xin ZHANG hong jianG Tian-hong ZHOU College of life Science and Technology,Jinan University,Guangzhou 510632,China CollegeoflifeScienceandTechnology JinanUniversityGuangzhou510632China

Seven sequence-specific ribozymes(M1GS RNAs)derived in vitro from the catalytic RNAsubunit of Escherichia coli RNase P and targeting the mRNAs transcribed by the UL54 gene encoding theDNA polymerase of human cytomegalovirus were screened from 11 ribozymes that were designed based onfour rules:(1)the NCCA-3′ terminal must be unpaired with the substrate;(2)the guide sequence(GS)mustbe at least 12 nt in length;(3)the eighth nucleotide must be U,counting from the site-1;and(4)around thecleavage site,the sites-1/+1/+2 must be U/G/C or C/G/*** investigation of the factors affecting thecleavage effect and the optimal ratio for M1GS/substrate was carried *** was determined that the optimalratio for M1GS/substrate was 2:1 and too much M1GS 1ed to substrate *** indicated *** M1GS that cleaved HCMV UL54 RNA segments in vitro were successfully designed and *** studies support the use of ribozyme M1GS as antisense molecules to silence HCMV mRNA in vitro,andusing the selection procedure as a general approach for the engineering of RNase P ribozymes.

关键词： ribonuclease P (RNase P) guide sequence HCMV DNA polymerase

The Synthesis, Crystal Structure and Thermoanalysis of N-Salicylidene Glycine Tripyridine Nickel( Ⅱ )

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Chemical Research in Chinese Universities 1992年第3期8卷 309-313页

作者： CUI Xue-gui, liU De-xin, SUN hong-jian, li Xiao-yan and li Shu-lan (Department of Applied Chemistry, Shandong University, Jinan, 250100)

Introduction Complexes of some Schiff bases with transition metals are widely used, for example, they can be used as anticancer medicine, preservative, complex reagent and the model of the biological oxygen carrier. So their syntheses, properties and application have been intensively studied. In order to study the relationship between the

关键词： Schiff base Nickel complex Crystal structure Thermoanalysis