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Expansive effects of aorta-gonad-mesonephros-derived stromal cells on hematopoietic stem cells from embryonic stem cells

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Chinese Medical Journal 2005年第23期118卷 1979-1986页

作者： FU Jin-rong LIU Wen-li ZHOU Yu-feng ZHOU jian-feng SUN han-ying LUO Li ZhanG Hen XU Hui-zhen Department of Hematology Tongji Hospital Tongji Medical SchoolHuazhong University of Science and Technology Wuhan 430030China

Background Hematopoietic stem cells （HSCs） give rise to all blood and immune cells and are used in clinical transplantation protocols to treat a wide variety of refractory diseases, but the amplification of HSCs has been difficult to achieve in vitro. In the present study, the expansive effects of aorta-gonad-mesonephros （AGM） region derived stromal cells on HSCs were explored, attempting to improve the efficiency of HSC transplantation in clinical practice.Methods The murine stromal cells were isolated from the AGM region of 12 days postcoitum （dpc） murine embryos and bone marrow（BM） of 6 weeks old mice, respectively. After identification with flow cytometry and immunocytochemistry, the stromal cells were co-cultured with ESCs-derived, cytokines-induced HSCs. The maintenance and expansion of ESCs-derived HSCs were evaluated by detecting the population of CD34^＋ and CD34^＋ Sca-1^＋ cells with flow cytometry and the blast colony-forming cells （BL-CFCs）, high proliferative potential colony-forming cells （HPP-CFCs） by using semi-solid medium colonial culture. Finally, the homing and hematopoietic reconstruction abilities of HSCs were evaluated using a murine model of HSC transplantation in vivo.Results AGM and BM-derived stromal cells were morphologically and phenotypically similar, and had the features of stromal cells. When co-cultured with AGM or BM stromal cells, more primitive progenitor cells （ HPP-CFCs ） could be detected in ESCs derived hematopoietic precursor cells, but BL-CFC＇ s expansion could be detected only when co-cultured with AGM-derived stromal cells. The population of CD34^＋ hematopoietic stem/ progenitor cells were expanded 3 times, but no significant expansion in the population of CD34^＋ Sca-1^＋ cells was noted when co-cultured with BM stromal cells. While both CD34^＋ hematopoietic stem/progenitor cells and CD34^＋ Sca-1^＋ cells were expanded 4 to 5 times respectively derived stromal cells, like BM-derived stromal cells, homing to BM in vivo. whe

关键词： hernatopoietic stern cells· embryonic stern cells· strornal cells

Totally laparoscopic Roux-en-Y cystojejunostomy as a sole treatment option for pancreatic pseudocysts： a report of four cases

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Chinese Medical Journal 2010年第15期123卷 2142-2144页

作者： SUN Yue-ming CAI Hui-hua BAI jian-feng ZHAO han-lin FU Zan MIAO Yi Department of Minimally Invasive Surgery First Affiliated Hospital Nanjing Medical University Nanjing Jiangsu 210029China

Pancreatic pseudocyst （PPC） is one of the most common complications of acute pancreatitis,pancreatic injury or *** the pseudocyst exceeds 6 cm in diameter and sustains over 6 weeks,1patients may suffer from abdominal pain, co-morbid illness, or the presence of abdominal *** such large pseudocyst is unlikely to resolve spontaneously, surgical treatment is necessary.

关键词： laparoscopy Roux-en- Y pancreatic pseudocyst

Virus-like particle of EV71: a model system for vaccine design

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Virus-like particle of EV71: a model system for vaccine desi...

Effects of Bifidobacterium lactis BLa80 on fecal and mucosal flora and stem cell factor/c-kit signaling pathway in simulated microgravity rats

第四届中国结构生物学学术讨论会

作者： Ke Lyu Hao-Yang Li Ya-Ling He jian-feng han Cheng-feng Qin Rong Chen Institute Pasteur of Shanghai Chinese Academy of Sciences State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and Epidemiology

Human Enterovirus 71（EV71） is recognized as the leading causative agent of hand-foot-and-mouth disease（HFMD） in Asia-Pacific region in recent years. There are still no approved antiviral drugs or vaccines against EV71 infection yet. We have developed an advanced platform for production of the virus-like particles（VLPs） for EV71 in Saccharomyces Cerevisiae. Crystallographic analysis on these VLPs at 3.65 A resolution indicated that the VLP is structurally similar to the empty particle produced from EV71-infected cells. Immunization with VLPs in mice elicited robust neutralization antibodies against EV71 and potent cellular immune responses. In vivo challenge experiments showed that the immune sera induced by VLP conferred protection in neonate mice against lethal EV71 challenge. Together, our study indicated that VLP from yeast is a potential vaccine candidate against EV71 infection. We have now taken a combinational approach including mutagenesis, x-ray crystallography, cryoEM and immunization study for the development of potent multivalent vaccine against HFMD.

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World Journal of Gastroenterology 2025年第1期31卷 93-109页

作者： Ping Zhang Ying Zhu Pu Chen Tong Zhou Zhe-Yi han Jun Xiao jian-feng Ma Wen Ma Peng Zang Ying Chen Graduate School Hebei North UniversityZhangjiakou 075000Hebei ProvinceChina Key Laboratory of Aerospace Nutrition and Food Engineering China Astronaut Research and Training CenterBeijing 100094China Department of Gastroenterology The Air Force Medical CenterBeijing 100142China Department of Blood Transfusion Air Force Medical CenterBeijing 100142China

BACKGROUND Simulated microgravity environment can lead to gastrointestinal motility *** pathogenesis of gastrointestinal motility disorders is closely related to the stem cell factor(SCF)/c-kit signaling pathway associated with intestinal flora and Cajal stromal ***,intestinal flora can also affect the regulation of SCF/c-kit signaling pathway,thus affecting the expression of Cajal stromal *** cells are the pacemakers of gastrointestinal *** To investigate the effects of Bifidobacterium lactis(***)BLa80 on the intestinal flora of rats in simulated microgravity and on the gastrointestinal motility-related SCF/c-kit *** The internationally recognized tail suspension animal model was used to simulate the microgravity environment,and 30 rats were randomly divided into control group,tail suspension group and drug administration tail suspension group with 10 rats in each group for a total of 28 *** tail group was given *** BLa80 by intragastric administration,and the other two groups were given water intragastric administration,the concentration of intragastric administration was 0.1 g/mL,and each rat was 1 mL/***&eosin staining was used to observe the histopathological changes in each segment of the intestine of each group,and the expression levels of SCF,c-kit,extracellular signal-regulated kinase(ERK)and p-ERK in the gastric antrum of each group were detected by Western blotting and *** fecal flora and mucosal flora of rats in each group were detected by 16S *** Simulated microgravity resulted in severe exfoliation of villi of duodenum,jejunum and ileum in rats,marked damage,increased space between villi,loose arrangement,shortened columnar epithelium of colon,less folds,narrower mucosal thickness,reduced goblet cell number and crypts,and significant improvement after probiotic *** microgravity reduced the expressions of SCF and c-kit,and increased the express

关键词： Simulated microgravity Rat Intestinal flora Gastrointestinal motility Stem cell factor/c-kit signaling pathway

Sonic hedgehog protein promotes bone marrow-derived endothelial pro-genitor cell proliferation,migration and VEGF production via PI 3-kinase/Akt signaling pathways

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Acta Pharmacologica Sinica 2006年第6期27卷 685-693页

作者： Jin-rong FU~2 Wen-li LIU~(2,4)jian-feng ZHOU~2 han-ying SUN~2 Hui-zhen XU~2 Li LUO~2 Heng ZhanG~2 Yu-feng ZHOU~3 2 Department of Hematology.Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China:3 Department of Pediatrics,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China Department of Hematology Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China Department of Pediatrics Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan China

Aim:To investigate the effects of Sonic hedgehog(shh)protein on bone marrow-derived endothelial progenitor cells(BM-EPC)proliferation,migration and vascu-lar endothelial growth factor(VEGF)production,and the potential signaling path-ways involved in these ***:Bone marrow-derived Flk-1~+ cells wereenriched using the MACS system from adult Kunming mice and then BM-EPCwas cultured in gelatin-coated culture *** effects of shh N-terminal pep-tide on BM-EPC proliferation were evaluated using the MTT colorimetric *** migration was assayed using a modified Boyden chamber *** of VEGF was determined by ELISA and immunofluorescence *** potential involvement of PKC and PI3K signaling pathways was exploredusing selective inhibitor or Western ***:The proliferation,migrationand VEGF production in BM-EPC could be promoted by endogenous shh N-terminal peptide at concentrations of 0.1μg/mL to 10 μg/mL,and could be inhib-ited by anti-shh ***-mediated proliferation and migration in BM-EPCcould be partly attenuated by ***-PI3-kinase expression in newlyseparated BM-EPC was low,and it increased significantly when exogenous shhN-terminal peptide was added,but could be attenuated by anti-human/mouse shhN-terminal peptide ***,the inhibitor of the PI3-kinase,but notthe inhibitor of the PKC,significantly inhibited the shh-mediated proliferation,migration and VEGF ***:Shh protein can stimulate bonemarrow-derived BM-EPC proliferation,migration and VEGF production,whichmay promote neovascularization to ischemic *** results also suggeststhat the PI3-kinase/Akt signaling pathways are involved in the angiogenic effectsof shh.

关键词： mouse Sonic hedgehog protein endothelial cells stem cells protein kinase C 1-phosphatidylinositol 3-kinase vascular endothelial growth factor A cell proliferation emigration immigration

基于绝对节点坐标法的柔性绳索体建模仿真研究

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科学技术与工程 2016年第31期16卷 1-7页

作者：沈剑韩峰陈放周峤北京理工大学爆炸科学与技术国家重点实验室北京100081

将绝对节点坐标法应用于柔性绳索体动力学建模和仿真的研究之中,利用绳单元建立了二维悬臂梁、三维柔性网和刚-柔耦合柔性网三种模型。借助Bathe积分策略和广义极小残量法(GMRES)对长时间历程、非对称大型稀疏矩阵方程组进行了分析求解... 详细信息

将绝对节点坐标法应用于柔性绳索体动力学建模和仿真的研究之中,利用绳单元建立了二维悬臂梁、三维柔性网和刚-柔耦合柔性网三种模型。借助Bathe积分策略和广义极小残量法(GMRES)对长时间历程、非对称大型稀疏矩阵方程组进行了分析求解,得到了三种模型的动力学仿真结果。通过对柔性绳索体的建模和仿真研究,确立了适用于大变形、大位移柔性绳索体动力学研究的方法,并提出了该研究领域未来发展方向应是绝对节点坐标法与各向异性材料本构关系的结合。

关键词：航天动力学柔性绳索体绝对节点坐标法刚度矩阵

Clinical manifestations and cerebral angiographic findings of moyamoya disease

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Journal of Pharmaceutical Analysis 2010年第2期22卷 131-134页

作者： jian-feng han1,Cheng-Tai Wang2,jian-Bo Yang1,feng Guo1,Zheng-Yi Li11. Department of Neurology,the First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061 2. No.2 Department of Neurology,Hospital of Chinese Traditional Medicine of Baoji,Baoji 721000,China. Department of Neurology First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China Department of Neurology Hospital of Chinese Traditional Medicine of Baoji Baoji 721000 China

Objective To study the clinical features and angiographic findings of moyamoya disease (MMD) as well as their relationship. Methods A total of 22 MMD patients received routine digital substraction angiography (DSA). The clinical manifestations and angiographic findings were analyzed. Results Clinical manifestations varied and each patient often had multiple symptoms,including cerebral infarction in 9 patients with an average age of 23.6 (13-39 years) and cerebral hemorrhage in 7 patients with an average age of 31.2 (28-46 years). Angiographic examination found that all the diseased sides showed MMD blood vessels. The patients who received encephalo-myo-arterio-synangiosis (EMAS) had better prognosis than those without receiving the treatment. Conclusion Cerebral infarction is frequent in children and adolescents with MMD,whereas cerebral hemorrhage is common in adults. DSA is a golden criterion for diagnosing MMD. Surgical treatment,EMAS blood supply reconstruction in particular,should be prescribed.

关键词： moyamoya disease digital subtraction angiography clinical characteristic

Bioinspired Passive Tactile Sensors Enabled by Reversible Polarization of Conjugated Polymers

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Nano-Micro Letters 2025年第1期17卷 361-377页

作者： feng He Sitong Chen Ruili Zhou hanyu Diao Yangyang han Xiaodong Wu School of Mechanical Engineering Sichuan UniversityChengdu 610065People’s Republic of China School of Software Engineering Chongqing University of Posts and TelecommunicationsChongqing 400065People’s Republic of China State Key Laboratory of Polymer Materials Engineering Sichuan UniversityChengdu 610065People’s Republic of China

Tactile perception plays a vital role for the human body and is also highly desired for smart prosthesis and advanced *** to active sensing devices,passive piezoelectric and triboelectric tactile sensors consume less power,but lack the capability to resolve static ***,we address this issue by utilizing the unique polarization chemistry of conjugated polymers for the first time and propose a new type of bioinspired,passive,and bio-friendly tactile sensors for resolving both static and dynamic ***,to emulate the polarization process of natural sensory cells,conjugated polymers(including poly(3,4-ethylenedioxythiophen e):poly(styrenesulfonate),polyaniline,or polypyrrole)are controllably polarized into two opposite states to create artificial potential *** controllable and reversible polarization process of the conjugated polymers is fully in situ ***,a micro-structured ionic electrolyte is employed to imitate the natural ion channels and to encode external touch stimulations into the variation in potential difference *** with the currently existing tactile sensing devices,the developed tactile sensors feature distinct characteristics including fully organic composition,high sensitivity(up to 773 mV N^(−1)),ultralow power consumption(nW),as well as superior *** demonstrations,both single point tactile perception(surface texture perception and material property perception)and two-dimensional tactile recognitions(shape or profile perception)with high accuracy are successfully realized using self-defined machine learning *** tactile sensing concept innovation based on the polarization chemistry of conjugated polymers opens up a new path to create robotic tactile sensors and prosthetic electronic skins.

关键词： Passive tactile sensors Reversible polarization of conjugated polymers Tactile perception Machine learning algorithm Object recognition

A novel active-passive protection strategy endows carbonyl iron with excellent intelligent anti-corrosion and microwave absorption dual functional characteristics

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Nano Research 2025年第1期18卷 487-500页

作者： Wei Tian Quanyuan feng Linbo Zhang Yinfan Liu Xian jian Longjiang Deng Institute of Microelectronics School of Information Science and TechnologySouthwest Jiaotong UniversityChengdu 611756China National Engineering Researching Centre of Electromagnetic Radiation Control Materials Key Laboratory of Multi-Spectral Absorbing Materials and Structures of Ministry of EducationState Key Laboratory of Electronic Thin Films and Integrated DevicesSchool of Electronic Science and EngineeringUniversity of Electronic Science and Technology of ChinaChengdu 611731China School of Materials and Energy University of Electronic Science and Technology of ChinaChengdu 611731China The Yangtze Delta Region Institute(Huzhou)&School of Electronic Science and Engineering University of Electronic Science and Technology of ChinaHuzhou 313001China

Carbonyl iron absorbers(CI)face significant challenges in practical applications,such as corrosion,interface bonding failure,detachment,and high maintenance ***,we have developed intelligent self-healing technology based on proactive/passive mechanisms via in situ synthesis of self-healing factors(polydopamine/benzotriazole(PDA/BTA))and physical barrier layers(SiO_(2)/1,1,1,3,3,3-hexamethyl disilazane,SiO_(2)/HMDS)to enhance corrosion resistance,while also being compatible with efficient microwave absorption *** unique multiscale structure gives full play to the utilization of the roles of each functional layer,including the intelligent self-healing features of PDA/BTA,physical shielding and spatial confinement characteristics of SiO_(2)/HMDS,and magnetic-dielectric synergistic mechanism resulted from the good impedance matching characteristics,the conduction loss,the interfacial polarization loss and natural *** asfabricated composites achieved an exceptional minimum reflection loss value of-55.4 dB at 10.7 GHz and the effective absorption band of 7.6 ***,it still exhibits obvious self-healing and corrosion resistance characteristics after 360 h corrosion treatment,ascribed to the self-healing mechanism of PDA/BTA and the blocking intervention effect of SiO_(2)/*** work is considered to pave the way for the synthesis of high-performance magnetic absorbers,especially in enhancing their intelligent self-healing ability in corrosive environments.

关键词： microwave absorption intelligent self-healing technology carbonyl iron multiscale structure magneticdielectric synergistic mechanism