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Intracellular accumulation of tau inhibits autophagosome formation by activating TIA1-amino acid-mTORC1 signaling

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Military Medical Research 2023年第2期10卷 175-190页

作者： Meng-Zhu Li En-Jie Liu Qiu-Zhi Zhou Shi-Hong Li Shi-Jie Liu Hai-Tao Yu Qi-Hang Pan fei Sun Ting He Wei-Jin Wang Dan Ke Yu-Qi Feng Jun Li jian-Zhi Wang Department of Pathophysiology School of Basic MedicineKey Laboratory of Education Ministry of China/Hubei Province for Neurological DisordersTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Neurosurgery the Central Hospital of WuhanTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430014China Department of Pathology the First Affiliated Hospital of Zhengzhou UniversityZhengzhou 450052China Department of Chemistry Wuhan UniversityWuhan 430072China Co-Innovation Center of Neuroregeneration Nantong UniversityNantong 226000JiangsuChina.

Background:Autophagy dysfunction plays a crucial role in tau accumulation and neurodegeneration in Alzheimer’s disease(AD).This study aimed to investigate whether and how the accumulating tau may in turn affect ***:The primary hippocampal neurons,N2a and HEK293T cells with tau overexpression were respectively starved and treated with vinblastine to study the effects of tau on the initiating steps of autophagy,which was analysed by Student’s two-tailed *** rapamycin and concanamycin A were employed to inhibit the mammalian target of rapamycin kinase complex 1(mTORC1)activity and the vacuolar H+-ATPase(v-ATPase)activity,respectively,which were analysed by One-way ANOVA with post hoc *** Western blotting,co-immunoprecipitation and immunofuorescence staining were conducted to gain insight into the mechanisms underlying the tau effects of mTORC1 signaling alterations,as analysed by Student’s two-tailed t-test or One-way ANOVA with post hoc *** autophagosome formation was detected by immunofuorescence staining and transmission electron *** amino acids(AA)levels were detected by high performance liquid chromatography(HPLC).Results:We observed that overexpressing human full-length wild-type tau to mimic AD-like tau accumulation induced autophagy *** studies revealed that the increased tau could bind to the prion-related domain of T cell intracellular antigen 1(PRD-TIA1)and this association significantly increased the intercellular level of amino acids(Leucine,P=0.0038;Glutamic acid,P=0.0348;Alanine,P=0.0037;Glycine,P=0.0104),with concordant upregulation of mTORC1 activity[phosphorylated eukaryotic translation initiation factor 4E-binding protein 1(p-4EBP1),P<0.0001;phosphorylated 70 kD ribosomal protein S6 kinase 1(p-p70S6K1),P=0.0001,phosphorylated unc-51-like autophagyactivating kinase 1(p-ULK1),P=0.0015]and inhibition of autophagosome formation[microtubuleassociated protein light chain 3 II(LC3 II),P=0.0073;LC3 pun

关键词： Tau Autophagy Amino acid pathway Mammalian target of rapamycin kinase complex 1(mTORC1) T cell intracellular antigen 1(TIA1)

贵州省遵义县南白镇人兽共患寄生蠕虫宿主动物的流行病学调查

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中华地方病学杂志 2016年第9期35卷 655-658页

作者：申万文邓菲高健安陈雪周必英遵义医学院医学与科技学院临床医学本科生遵义563000 遵义医学院医学与科技学院寄生虫学教研室遵义563000

目的调查贵州省遵义县南白镇家犬蠕虫卵感染情况、溪蟹种类及携带肺吸虫囊蚴情况。方法2014年11月至2015年9月，选择遵义县南白镇谢家村和龙泉行政村作为调查点，以当地家犬和溪蟹为对象，采用自然沉淀法检查家犬粪便蠕虫卵；基于模式标... 详细信息

目的调查贵州省遵义县南白镇家犬蠕虫卵感染情况、溪蟹种类及携带肺吸虫囊蚴情况。方法2014年11月至2015年9月，选择遵义县南白镇谢家村和龙泉行政村作为调查点，以当地家犬和溪蟹为对象，采用自然沉淀法检查家犬粪便蠕虫卵；基于模式标本Holotype，基础鉴定溪蟹种类；捣碎溪蟹，光镜下检测肺吸虫囊蚴。结果共采集家犬新鲜粪便53份，检出犬复孔绦虫卵、犬弓首蛔虫卵、曼氏迭宫绦虫卵，共3种蠕虫卵。共捕捉溪蟹42只（雌性14只，含幼体2只；雄性28只，含幼体9只），均为遵义华溪蟹，肺吸虫囊蚴检测均为阴性。结论遵义县南白镇仅存在遵义华溪蟹，种类较单一；溪蟹中未查见肺吸虫囊蚴，提示当地不属于肺吸虫病流行区；家犬粪便仅查获3种蠕虫卵。

关键词：狗粪便蠕虫肺吸虫

A Soft Sensing Method for Operation Optimization of Coke Dry Quenching Process 36

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A Soft Sensing Method for Operation Optimization of Coke Dry...

第36届中国控制会议

作者： jian-Guo Wang Zhi-Duo Cao Li-Lan Liu Bang-Hua Yang Shi-Wei Ma Min-Rui fei Zhi-Fu Deng Yuan Yao Yi-Ping Wu School of Mechatronical Engineering and Automation Shanghai UniversityShanghai Key Lab of Power Station Automation Technology Department of Chemical Engineering “Tsing-Hua University” Shanghai Baosight Software Co Ltd

ISBN: (纸本)9781538629185

Based on the actual data analysis, it is found in this paper that the supplementary air flow rate in the CDQ operation didn’t follow the variation of the discharge rate of incandescent coke well, which results in the concentration increase of combustible gas in the exhaust gas and the decrease of economic efficiency. The correlation analysis results show that the introduced derived variables are more useful than some plain variables for the purpose of prediction. Next, to handle the contradiction between the steam productivity and the coke burning loss, a new economic efficiency index is introduced by synthesizing the two competing aspects. A kind of soft sensor of economic efficiency is put forward by combining nonnegative garrote（NNG） variable selection algorithm with the autoregressive integrated moving average（ARIMA） model, which gives a good solution to the economic efficiency real-time prediction problem of CDQ system. Then, the implementation of model-based optimization is studied based on the actual operation data. The results show that there exists large room for economic efficiency promotion.

关键词： Soft sensor Coke dry quenching Data-driven Variable selection Autoregressive integrated moving average

来源： cnki会议评论

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直接面向交易的跨省区输电费用分摊

电力系统保护与控制 2012年第7期40卷 112-116页

作者：高效彭建春国网能源研究院北京100052 湖南大学电气与信息工程学院湖南长沙410082

对联营和双边交易混存的跨省区电力市场,提出考虑无功潮流影响的输电费用分摊方法。设计了描述任意复杂的联营和双边交易的数学模型。基于交易引起的支路电流响应向量及功率向量,提出了将交易等值成复功率元件后各交易使用线路份额。提... 详细信息

对联营和双边交易混存的跨省区电力市场,提出考虑无功潮流影响的输电费用分摊方法。设计了描述任意复杂的联营和双边交易的数学模型。基于交易引起的支路电流响应向量及功率向量,提出了将交易等值成复功率元件后各交易使用线路份额。提出基于使用度将跨省区输电费用直接分配给所有交易的方法。通过IEEE14节点的实例仿真验证了该方法的合理性和可行性,为跨省区输电定价提供了一种有效方法。

关键词：跨省区输电费用分摊交易使用度

p53、p21^Gip1/WAF1和Gadd45α在乳腺癌中的表达及其意义

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中华医学杂志 2012年第34期92卷 2389-2393页

作者：高其忠费伯健齐晓薇浦勇冯一中苏州大学附属第四医院普通外科江苏省无锡市214062 苏州大学附属第四医院病理科江苏省无锡市214062 苏州大学医学部基础医学与生物科学学院病理教研室苏州大学附属第二医院病理科

目的探讨p53、p21^Gip1/WAF1和Gadd45α在乳腺癌中的表达情况及其与临床病理参数和预后的关系。方法采用组织芯片和免疫组化sP法检测133例乳腺癌组织及其癌旁正常组织中p53、p21^Gip1/WAF1和Gadd45α的表达，应用Spearman秩和相关分析... 详细信息

目的探讨p53、p21^Gip1/WAF1和Gadd45α在乳腺癌中的表达情况及其与临床病理参数和预后的关系。方法采用组织芯片和免疫组化sP法检测133例乳腺癌组织及其癌旁正常组织中p53、p21^Gip1/WAF1和Gadd45α的表达，应用Spearman秩和相关分析两者之间的相关性，应用***法和Cox多因素回归模型分析与预后的关系。结果p53、p21^Gip1/WAF1和Gadd45α在乳腺癌组织中的阳性表达率（58．6％和47．4％）高于正常乳腺组织（O％）（P〈0．05）；Gadd45et在乳腺癌组织中的阳性表达率（41．4％）低于正常乳腺组织（P〈0．05）。v53与淋巴结转移、临床分期相关，差异均有统计学意义（均P〈0．05），p53、p21^Gip1/WAF1与组织分化程度、局部复发、C—erbB-2状态相关，Gadd45a与组织分化程度、腋窝淋巴结转移、远处转移组、ER／PR阳性状态相关。等级相关分析结果提示p53、p21^Gip1/WAF1表达与p53表达呈正相关，Gadd45a表达与p53表达呈负相关，p53、p21^Gip1/WAF1表达与Gadd45ct表达无相关性。结合随访资料，***分析显示p53、p21^Gip1/WAF1、Gadd45a、p53、淋巴结转移、C—erbB-2、TNM分期与预后相关，Cox多因素回归分析提示p53、p21^Gip1/WAF1、C—erbB-2、TNM分期是独立的预后参数，Kaplan-Meier分析提示p53、p21^Gip1/WAF1和p53（＋）Gadd45ct（-）与乳腺癌不良预后相关。结论p53、p21^Gip1/WAF1和Gadd45α的表达与乳腺癌的病理参数及预后相关，p53（＋）Gadd45ct（-）和p53、p21^Gip1/WAF1（＋）可作为独立的预后参数。

关键词：乳腺肿瘤 p53、p21^Gip1/WAF1 Gadd45α

Structural Distortion and Defects in Ti_(3)AlC_(2) irradiated by Fe and He Ions

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Chinese Physics Letters 2018年第2期35卷 48-51页

作者： Li-Long Pang Bing-Sheng Li Tie-Long Shen Xing gao Xue-Song Fang Ning gao Cun-Feng Yao Zong-Fang Wei Ming-Huan Cui jian-Rong Sun Hai-Long Chang Wen-Hao He Qing Huang Zhi-Guang Wang Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000 University of Chinese Academy of Sciences Beijing 100049 Ningbo Institute of Material Technology and Engineering Chinese Academy of SciencesNingbo 315201

Ti_(3)AlC_(2)samples are irradiated in advance by 3.5 MeV Fe-ion to the fluence of 1.0×10^(16)ion/cm^(2),and then are implanted by 500 keV He-ion with the fluence of 1.0×10^(17)ion/cm^(2)at room *** irradiated samples are investigated by grazing incidence x-ray diffraction(GIXRD)and transmission electron microscopy(TEM).GIXRD results show serious structural distortion,but without amorphization in the irradiated ***-ion irradiation and He-ion implantation create much more serious structural distortion than single Fe-ion *** results reveal that there are a large number of defect clusters in the damage region,and dense spherical He bubbles appear in the He depositional *** seems that the pre-damage does not influence the growth of He bubbles,but He-ion implantation influences the pre-created defect configurations.

关键词： Ion bombardment

Characterization of recombinant humanized collagen type III and its influence on cell behavior and phenotype

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Journal of Leather Science and Engineering 2022年第1期4卷 452-464页

作者： Jing Wang Hong Hu jian Wang He Qiu Yongli gao Yang Xu Zhanhong Liu Yajun Tang Lu Song John Ramshaw Hai Lin Xingdong Zhang National Engineering Research Center for Biomaterials Sichuan UniversityChengdu 610064SichuanChina College of Biomedical Engineering Sichuan UniversityChengdu 610064SichuanChina Shanxi Jinbo Pharmaceutical Co. Ltd.Taiyuan 030031ShanxiChina West China School/Hospital of Stomatology Sichuan UniversityChengdu 610064SichuanChina Swinburne University of Technology MelbourneAustralia.

Collagen made a tremendous impact in the field of regenerative medicine as a bioactive *** decades,collagen has been used not only as a scaffolding material but also as an active component in regulating cells’biological behavior and ***,animal-derived collagen as a major source suffered from problems of immunogenicity,risk of viral infection,and the unclear relationship between bioactive sequence and *** humanized collagen(rhCol)provided alternatives for regenerative medicine with more controllable ***,the characterization of rhCol and the interaction between rhCol and cells still need further investigation,including cell behavior and *** current study preliminarily demonstrated that recombinant humanized collagen type III(rhCol III)conformed to the theoretical amino acid sequence and had an advanced structure resembling bovine ***,rhCol III could facilitate basal biological behaviors of human skin fibroblasts,such as adhesion,proliferation and *** III was beneficial for some extracellular matrix-expressing cell *** study would shed light on the mechanism research of rhCol and cell interactions and further understanding of effectiveness in tissue regeneration.

关键词： Recombinant collagen Collagen type III Advanced structure Cell behavior Cell phenotype

近百年来长江荆江段牛轭湖沉积的孢粉、炭屑特征及其环境意义——以天鹅洲、尺八湖为例

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长江流域资源与环境 2017年第10期26卷 1630-1640页

作者：贾铁飞高鑫王峰上海师范大学地理系上海200234 华东师范大学河口海岸学国家重点实验室上海200062

根根据长江荆江段沿江牛轭湖天鹅洲和尺八沉积钻孔柱样,在建立了高分辨率的年代框架的基础上,分别进行了沉积物孢粉、炭屑分析,并结合荆州市1955~2012年的气候数据、史志记载的洪水和火灾等环境事件进行了综合分析。结果显示:天鹅洲、... 详细信息

根根据长江荆江段沿江牛轭湖天鹅洲和尺八沉积钻孔柱样,在建立了高分辨率的年代框架的基础上,分别进行了沉积物孢粉、炭屑分析,并结合荆州市1955~2012年的气候数据、史志记载的洪水和火灾等环境事件进行了综合分析。结果显示:天鹅洲、尺八两湖泊钻孔沉积物的孢粉和炭屑特征均较好地响应了近百年来湖区周边的环境变化;牛轭湖在裁断成湖之前、成湖之后的孢粉、炭屑特征不同:成湖之前,沉积物总孢粉浓度与乔木、灌木孢粉浓度都相对较低,水生植物和蕨类植物孢粉浓度相对较高,成湖之后乔木孢粉浓度仍呈减小趋势,但灌木、水生植物孢粉浓度呈波动增加趋势;利用沉积物炭屑浓度与孢粉浓度的比值(C/P值)指标,可以较好地指示自然山火与人类聚居地火灾等燃烧事件在沉积物中的记录,而且综合利用C/P值与沉积物孢粉特征,有可能厘清长江全流域洪水与局地洪涝事件的沉积记录。

关键词：牛轭湖沉积物分析环境事件孢粉、炭屑浓度 C/P值

Schiff碱铜(Ⅱ)络合物和甘油-聚乙烯微胶囊改性超高分子量聚乙烯的磨损机理研究

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摩擦学学报 2006年第6期26卷 599-602页

作者：高新蕾李健高万振武汉工业学院化学与环境工程系湖北武汉430023 武汉材料保护研究所湖北武汉430030

将所合成的乙二胺缩水杨醛Sch iff碱铜(Ⅱ)络合物和甘油-聚乙烯微胶囊与超高分子量聚乙烯(UHMWPE)共混制备出改性UHMWPE材料,利用销-盘式摩擦磨损试验机评价Sch iff碱铜(Ⅱ)络合物和甘油-聚乙烯微胶囊改性UHMWPE/GCr15钢配副在高速干摩... 详细信息

将所合成的乙二胺缩水杨醛Sch iff碱铜(Ⅱ)络合物和甘油-聚乙烯微胶囊与超高分子量聚乙烯(UHMWPE)共混制备出改性UHMWPE材料,利用销-盘式摩擦磨损试验机评价Sch iff碱铜(Ⅱ)络合物和甘油-聚乙烯微胶囊改性UHMWPE/GCr15钢配副在高速干摩擦条件下的摩擦磨损性能,利用扫描电子显微镜观察其磨损表面形貌,采用电子能谱仪分析磨损表面的主要元素组成并探讨其磨损机理.结果表明,由于其独特的自身选择性转移效应使得耐磨性提高,在高速干摩擦条件下没有严重的粘着磨损.

关键词： UHMWPE 乙二胺缩水杨醛Schiff碱铜(Ⅱ)络合物磨损表面分析