检索结果-南通市图书馆

Gene knockdown by structure defined single-stem loop small non-coding RNAs with programmable regulatory activities

Synthetic and Systems Biotechnology 2023年第1期8卷 86-96页

作者： yang Wang Guobin Yin Huanjiao Weng Luyao Zhang Guocheng du jian Chen Zhen Kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxiChina

Gene regulation by trans-acting small RNAs(sRNAs)has considerable advantages over other gene regulation ***,synthetic sRNAs mainly take natural sRNAs(MicC or SgrS)as backbones and comprise three functional elements folding into two or more stem-loop structures:an mRNA base pairing region,an Hfq-binding structure,and a rho-independent *** to limited numbers of natural sRNAs and complicated backbone structures,synthetic sRNAs suffer from low activity programmability and poor structural ***,natural sRNA backbone sequences may increase the possibility of unwanted ***,we present a bottom-up approach for creating structure defined single-stem loop small non-coding RNAs(ssl-sRNAs),which contain a standardized scaffold of a 7 bp-stem-4 nt-loop-polyU-tail and a 24 nt basing pairing region covering the first eight ***,ssl-sRNA requires no independent Hfq-binding structure,as the polyU tail fulfills the roles of binding Hfq.A thermodynamic-based scoring model and a web server sslRNAD(http://***/)were developed for automated design of ssl-sRNAs with well-defined structures and programmable ***-sRNAs displayed weak polar effects when regulating polycistronic *** ssl-sRNA designed by sslRNAD showed regulatory activities in both Escherichia coli and Bacillus subtilis.A streamlined workflow was developed for the construction of customized ssl-sRNA and ssl-sRNA *** examples,the *** cell morphology was easily modified and new target genes of ergothioneine biosynthesis were quickly identified with ***-sRNA and its designer sslRNAD enable researchers to rapidly design sRNAs for knocking down target genes.

关键词： Regulatory RNA Metabolic engineering Synthetic biology De novo design Ergothioneine

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Mutual-structure weighted guided image filtering for depth map restoration

引用

Optoelectronics Letters 2025年第1期21卷 51-56页

作者： LIU Zijian SONG jian CHEN Quanmin XU jiangtao Tianjin Key Laboratory of Imaging and Sensing Microelectronic Technology School of Microelectronics Tianjin University Spikesee Technology Co. Ltd.

Although guided image filtering(GIF) is known for preserving edges and fast computation, it may produce inaccurate outputs in depth map restoration. In this paper, a novel confidence-weighted GIF called mutual-structure weighted GIF(MSWGIF) is proposed, which replaces the mean filtering strategy in GIF during handling overlapping windows. The confidence value is composed of a depth term and a mutual-structure term, where the depth term is utilized to protect the edges of the output, and the mutual-structure term helps to select accurate windows during the structure characteristics of the guidance image are transferred to the output. Experimental results show that MSWGIF reduces the root mean square error(RMSE) by an average of 12.37%, and the average growth rate of correlation(CORR) is 0.07% on average. Additionally, the average growth rate of structure similarity index measure(SSIM) is 0.34%.

关键词： Wiener filtering

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Two new compounds from Coriaria nepalensis

引用

Chinese Chemical Letters 2011年第9期22卷 1078-1080页

作者： jian Hong yang jian Xin Pu Xue du Hai Bo Zhang Wei Lie Xiao Han Dong Sun State Key Laboratory of Phytochemistry and Plant Resources in West China Kunming Institute of Botany Chinese Academy of Sciences Kuming 650204 China

Two new compounds. corialins A （1） and B （2） were isolated from Coriaria nepulensis Wall. These new compounds were established as 7-hydroxy-3-[2,3-acetonidc-（3-methylhutane）] coumarin （1） and 3-O-β-D-glucopyr... 详细信息

关键词： Coriaria nepalensis Corialin A Corialin B NMR techniques

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Design of artificial small regulatory trans-RNA for gene knockdown in Bacillus subtilis

引用

Synthetic and Systems Biotechnology 2023年第1期8卷 61-68页

作者： Guobin Yin Anqi Peng Luyao Zhang yang Wang Guocheng du jian Chen Zhen Kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China

Bacillus subtilis as the Gram-positive model bacterium has been widely used in synthetic biology and biotechnology while the regulatory RNA tools for *** are still not fully ***,a bottom-up approach is proposed for designing artificial trans-acting *** engineering the intrinsic sRNA SR6,a minimized core scaffold structure consisting of an 8 bp stem,a 4 nt loop,and a 9 nt polyU tail was generated and proven to be sufficient for constructing sRNAs with strong repression activity(83%).Moreover,we demonstrate this artificial sRNA system functions well in an hfq-independent manner and also achieves strong repression efficiency in Escherichia coli(above 80%).A structure-based sRNA design principle was further developed for the automatic generation of custom sRNAs with this core scaffold but various sequences,which facilitates the manipulation and avoids structure disruption when fusing any base-pairing *** applying these auto-designed sRNAs,we rapidly modified the cell morphology and biofilm formation,and regulated metabolic flux toward acetoin *** sRNA system with cross-species regulatory activities not only enriched the gene regulation toolkit in synthetic biology for *** and *** but also enhanced our understanding of trans-acting sRNAs.

关键词： Gene knockdown Posttranscriptional regulation Small noncoding RNA Synthetic biology Cross-species Bacillus subtilis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Stability prediction of hard rock pillar using support vector machine optimized by three metaheuristic algorithms

引用

International Journal of Mining Science and Technology 2023年第8期33卷 1019-1036页

作者： Chuanqi Li jian Zhou Kun du Daniel Dias School of Resources and Safety Engineering Central South UniversityChangsha 410083China Laboratory 3SR CNRS UMR 5521Grenoble Alpes UniversityGrenoble 38000France

Hard rock pillar is one of the important structures in engineering design and excavation in underground *** and convenient prediction of pillar stability is of great significance for underground space *** paper aims to develop hybrid support vector machine(SVM)models improved by three metaheuristic algorithms known as grey wolf optimizer(GWO),whale optimization algorithm(WOA)and sparrow search algorithm(SSA)for predicting the hard rock pillar *** integrated dataset containing 306 hard rock pillars was established to generate hybrid SVM *** parameters including pillar height,pillar width,ratio of pillar width to height,uniaxial compressive strength and pillar stress were set as input *** global indices,three local indices and the receiver operating characteristic(ROC)curve with the area under the ROC curve(AUC)were utilized to evaluate all hybrid models’*** results confirmed that the SSA-SVM model is the best prediction model with the highest values of all global indices and local ***,the performance of the SSASVM model for predicting the unstable pillar(AUC:0.899)is not as good as those for stable(AUC:0.975)and failed pillars(AUC:0.990).To verify the effectiveness of the proposed models,5 field cases were investigated in a metal mine and other 5 cases were collected from several published *** validation results indicated that the SSA-SVM model obtained a considerable accuracy,which means that the combination of SVM and metaheuristic algorithms is a feasible approach to predict the pillar stability.

关键词： Underground pillar stability Hard rock Support vector machine Metaheuristic algorithms

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Lie transformation on shortcut to adiabaticity in parametric driving quantum systems

引用

Chinese Physics B 2021年第6期30卷 111-115页

作者： jian-jian Cheng Yao du Lin Zhang School of Physics and Information Technology Shaanxi Normal UniversityXi'an 710119China

Shortcut to adiabaticity(STA) is a speedway to produce the same final state that would result in an adiabatic, infinitely slow process. Two typical techniques to engineer STA are developed by either introducing auxiliary counterdiabatic fields or finding new Hamiltonians that own dynamical invariants to constraint the system into the adiabatic paths. In this paper,an efficient method is introduced to naturally cover the above two techniques with a unified Lie algebraic framework and neatly remove the design difficulties and loose assumptions in the two techniques. A general STA scheme for different potential expansions concisely achieves with the aid of squeezing transformations.

关键词： shortcut to adiabaticity parametric driving Lewis–Riesenfeld invariants

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Overexpression of the neuroglobin gene delivered by ultrasound-targeted microbubble destruction protects SH-SY5Y cells against cobalt chloride induced hypoxia

引用

Neural Regeneration Research 2011年第25期6卷 1947-1953页

作者： Qian yang Dianwen Gao Qingzhu Nie Zhengang Cai jian du Lujuan Shan Yuejian Liu Department of Ophthalmology Shengjing Hospital Affiliated to China Medical University Shenyang 110004 Liaoning Province China Department of General Surgery First Hospital Affiliated to Dalian Medical University Dalian 116021 Liaoning Province China Experimental Research Center First Hospital Affiliated to Dalian Medical University Dalian 116021 Liaoning Province China

In this study, we examined the effects of neuroglobin gene （Ngb） transfection into SH-SY5Y cells, using ultrasound-targeted microbubble destruction （UTMD）, on cobalt chloride-induced hypoxia. With an ultrasound intensity of 0.8 W/cm2, a 60-second exposure duration, 50% duty cycle, and 20% microbubble concentration, pAcGFP1-C1-Ngb-transfected cells exhibited the highest cell viability and transfection efficiency. The efficiency of plasmid delivery was significantly higher with UTMD than transfection with plasmid alone, transfection with plasmid using microbubbles, or transfection of plasmid by ultrasound. In addition, during cobalt chloride-induced hypoxia, caspase-3 activity in pAcGFP1-C1-Ngb-transfected cells was significantly lower than in untransfected cells. Ngb protein and mRNA expression were significantly higher in cells transfected by UTMD than in cells transfected with the other methods. These results demonstrate that UTMD can very efficiently mediate exogenous gene delivery, and that Ngb overexpression protects cells against cobalt chloride-induced hypoxia.

关键词： ultrasound-targeted microbubble destruction neuroglobin gene therapy recombinant plasmid SH-SY5Y cells neural regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Simultaneous increase in remanence and coercivity during grain refining of Nd-Fe-B deformed magnets

引用

Journal of Rare Earths 2022年第11期40卷 1763-1771,I0004页

作者： Fengqing Wang Kanghua Chen Juan du jian Zhang CAS Key Laboratory of Magnetic Materials and Devices Ningbo Institute of Materials Technology and EngineeringChinese Academy of Sciences(CAS)Ningbo 315201China Zhejiang Province Key Laboratory of Magnetic Materials and Application Technology Ningbo Institute of Materials Technology and EngineeringChinese Academy of SciencesNingbo 315201China State Key Laboratory for Powder Metallurgy Central South UniversityChangsha 410083China Institute of Materials Shanghai UniversityShanghai 200444China

To explore the feasibility of deep grain-size refinement in overcoming the problem of traditional B-Htrade-off in bulk permanent magnets,the effect of deformation temperature on the texture strength,grain refining,and magnetic properties of slow-deformed Nd-Fe-B magnets was systematically *** a result,strong textures with gradual grain refining from conventionally large size of D=650 nm to small nanoscale size of D=53 nm(Ddenotes the lateral size of the aligned plate-like grains)are observed as the temperature reduces from T=650℃to T=450℃.Moreover,magnetic observations show a simultaneous increase in remanence and coercivity as the grain refines from D=650 nm to D=127 *** increase in coercivity results from the grain size refinement as well as the smaller aspect ratio of the plate-like grains,and the increase in remanence results from the improved texture *** compared with the D=650 nm magnets,the simultaneous increase in remanence and coercivity leads to optimum enhancements of 57%in coercivity,10%in remanence,and 25%in energy product,which demonstrate the feasibility of near-nano scale grain refinement in overcoming the traditional B-Htrade-off for improved(BH)*** failure of higher coercivities in the D≤80 nm magnets is closely related with the defect effects of the grain *** explaining the grain refining and texture changing behavior were also discussed.

关键词： NdFeB magnets Grain size Plastic deformation Texture Coercivity Rare earths

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Convergence of Broadband and Broadcast/Multicast in Maritime Information Networks

引用

Tsinghua Science and Technology 2021年第5期26卷 592-607页

作者： Jun du jian Song Yong Ren Jintao Wang the Department of Electronic Engineering Tsinghua UniversityBeijing 100084China the Institute for Ocean Engineering Tsinghua UniversityBeijing 100084 Peng Cheng Laboratory Shenzhen 518055China the Beijing National Research Center for Information Science and Technology Tsinghua UniversityBeijing 100084 the Key Laboratory of Digital TV System of Guangdong Province and Shenzhen City Research Institute of Tsinghua UniversityShenzhen 518057China

Recently,the fifth-generation(5G)of wireless networks mainly focuses on the terrestrial ***,the well-developed emerging technologies in 5G are hardly applied to the maritime communications,resulting from the lack of communication infrastructure deployed on the vast ocean,as well as different characteristics of wireless propagation environment over the sea and maritime user *** satisfy the expected plethora of broadband communications and multimedia applications on the ocean,a brand-new maritime information network with a comprehensive coverage capacity in terms of all-hour,all-weather,and all-sea-area has been expected as a revolutionary paradigm to extend the terrestrial capacity of enhanced broadband,massive access,ultra-reliable,and low-latency to the vast *** considering the limited available resource of maritime communication infrastructure,the convergence of broadband and broadcast/multicast can be regarded as a possible yet practical solution for realizing an efficient and flexible resource configuration with high quality of ***,according to such multi-functionality and all-coverage maritime information network,the monitoring and sensing of vast ocean area relying on massive Ocean of Things and advanced radar techniques can be also *** these issues above,this study proposes a Software Defined Networking(SDN)based Maritime Giant Cellular Network(MagicNet)architecture for broadband and multimedia *** on this network,the convergence techniques of broadband and broadcast/multicast,and their supporting for maritime monitoring and marine sensing are also introduced and surveyed.

关键词： Maritime Giant Cel ular Network(Magic Net) integrated broadcast-multicast-unicast communications maritime multimedia services Ocean of Things(OoT) al-coverage

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Engineering strong and stress-responsive promoters in Bacillus subtilis by interlocking sigma factor binding motifs

引用

Synthetic and Systems Biotechnology 2019年第4期4卷 197-203页

作者： yang Wang Yanan Shi Litao Hu Guocheng du jian Chen Zhen Kang The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationJiangnan UniversityWuxi214122China

Prokaryotic gene expression is largely regulated on transcriptional levels with the involvement of promoters,RNA polymerase and sigma *** new promoters to customize gene transcriptional regulation becomes increasingly demanded in synthetic biology and *** this study,we designed synthetic promoters in the Gram-positive model bacterium Bacillus subtilis by interlocking the binding motifs ofσA for house-keeping gene expression and that of two alternative sigma factorsσH andσB which are involved in responding post-exponential growth and general stress,*** developed promoters are recognized by multiple sigma factors and hence generate strong transcriptional strength when host cells grow under normal or stressed *** green fluorescent protein as the reporter,a set of strong promoters were identified,in which the transcription activities of PHA-1,PHAB-4,PHAB-7 were 18.6,4.1,3.3 fold of that of the commonly used promoter P43,***,some of the promoters such as PHA-1,PHAB-4,PHAB-7,PBA-2 displayed increased transcriptional activities in response to high salinity or low *** promoters developed in this study should enrich the biotechnological toolboxes of ***.

关键词： Synthetic biology Transcription Promoter engineering Stress-responsive Bacillus subtilis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：