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Different expression of dectin-1 and Toll-like receptor 2 in the lungs of different immune status mice infected with Aspergillus fumigatus

中国化学会第29届学术年会

作者： jian wan Lingling Feng Yanliang Ren College of Chemistry Central China Normal University Key Laboratory of Pesticide & Chemical Biology (CCNU) MOE

In the first part,the energy transfer pathways in trimeric and hexameric aggregation state of cyanobacteria C-phycocyanin（C-PC）were investigated in the framework of F?rster *** when the protonation of phycocyanobilins and their long-and short-rang interactions were properly taken into account,the calculated energy transfer rates were in good agreement with the experimental results of C-PC monomer and *** C-PC hexamer,an additional energy flow were predicted to be fromβ-155 in top trimer to adjacentβ-155 in bottom trimer（Figure1）.Fructose-1,6-/sedoheptulose-1,7-bisphosphatase（C-FBP/SBPase）is a unique enzyme for cyanobacteria with essential roles in Calvin *** and biochemical characterization of C-FBP/SBPase from the Synechocystis strain 6803 have been reported in the second *** a systematically structure-centric approach,we identified the first set of novel dual-target inhibitors against cyanobacterial fructose-1,6-bisphosphate aldolase（FBA）and C-FBP/*** obtained inhibitors show micromolar-range activities in vitro and in vivo,and may open a promising route towards developing environmentally acceptable chemical control for cyanobacteria blooms treatment.

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Chinese Medical Journal 2009年第17期122卷 2017-2021页

作者： YANG jian-xun LIU wan LU Qiao-yun wan Zhe wanG Xiao-hong LI Ruo-yu Department of Dermatology Research Center of MedicalMycology Peking University First Hospital Beijing 100034China

Background Invasive pulmonary aspergillosis （IPA） is a severe and frequently fatal disease in patients receiving treatment with immunosuppressive agents such as cyclophosphamide. Aspergillus fumigatus （A. fumigatus） now is a leading cause of IPA. Dectin-1 and Toll-like receptor 2 （TLR2） are important pattern recognition receptors involved in immune responses to A. fumigatus in vitro. However, the expression of the two receptors during the infection of A. fumigatus in vivo is not completely understood. The effects of cyclophosphamide treatment on the expression of the receptors need to be further studied. Methods We established different immune status in mice models with or without A. fumigatus infection. On days 1, 3 and 5 post inoculation, pulmonary tissues from mice of the different groups were harvested. Dectin-1 and TLR2 mRNA expression in the lungs of the mice were investigated by real-time PCR. The pulmonary fungal burden in the mice with A. fumigatus infection was also evaluated. Results In the immunocompetent mice, three days after A. fumigatus inoculation, dectin-1 and TLR2 expression increased markedly compared with the normal control group. Cyclophosphamide inhibited the clearance of pathogens and the expression of dectin-1 with or without A. fumigatus infection in the lungs as well. There was no statistical difference in TLR2 expression between the different immune status groups. Conclusions Our results suggest that in vivo, dectin-1 and TLR2 are activated during the experimental period which would provide a broad range of possibilities for a specific and effective inflammatory response to kill A. fumigatus. Inhibition of dectin-1 expression may be one of the mechanisms of cyclophosphamide in the development of IPA. Chin Med J 2009; 122（17）：2017-2021

关键词： Aspergillus fumigatus dectin-1 Toll-like receptor 2 real-time polymerase chain reaction

Studies on the Energy Transfer Pathways in the Light-Harvesting Complex Ⅱ and the Key Regulatory Enzymes in Calvin Cycle of Cyanobateria Photosystem

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Studies on the Energy Transfer Pathways in the Light-Harvest...

Inhibitor Screening based on Cyanobacteria FBP/SBPase

中国化学会第十二届全国量子化学会议

作者： jian wan Lingling Feng Yanliang Ren College of Chemistry Central China Normal University China Key Laboratory of Pesticide ＆ Chemical Biology （CCNU）

In the first part,the energy transfer pathways in trimeric and hexameric aggregation state of cyanobacteria C-phycocyanin（C-PC）were investigated in the framework of F?rster *** when the protonation of phycocyanobilins and their long-and short-rang interactions were properly taken into account,the calculated energy transfer rates were in good agreement with the experimental results of C-PC monomer and *** C-PC hexamer,an additional energy flow was predicted to be fromβ-155 in top trimer to adjacentβ-155 in bottom trimer（Figure1）.Fructose-1,6-/sedoheptulose-1,7-bisphosphatase（C-FBP/SBPase）is a unique enzyme for cyanobacteria with essential roles in Calvin *** and biochemical characterization of C-FBP/SBPase from the Synechocystis strain 6803 have been reported in the second *** a systematically structure-centric approach,we identified the first set of novel dual-target inhibitors against cyanobacterial fructose-1,6-bisphosphate aldolase（FBA）and C-FBP/*** obtained inhibitors show micromolar-range activities in vitro and in vivo,and may open a promising route towards developing environmentally acceptable chemical control for cyanobacteria blooms treatment.

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Inhibitor Screening based on Cyanobacteria FBP/SBPase

Genotyping the Heading Date of Male-Sterile Rice Line II-32A

第十届全国化学生物学学术会议

作者： Lingling Feng Yao Sun jian wan Key Laboratory of Pesticide ＆ Chemical Biology of Ministry of Education College of ChemistryCentral China Normal University

Cyanobacteria produce harmful algal blooms（HABs） and then cause water pollution worldwide and a hazard to animal and human health. Cyanobacterial fructose-1,6-/sedoheptulose-1,7-bisphosphatase（Cy-FBP/SBPase） is a special and important enzyme and is thought as a potential enzymatic target for screening of novel inhibitors that combat HABs. The key question is to research the interactional mechanism between new target cy-FBP/SBPase and its novel inhibitor. Here, we firstly crystallized and determined the structure of cy-FBP/SBPase complex. The results indicate its special molecular catalytic mechanisms different from other FBPase in this enzyme family. Then a series of novel hit compounds were selected by using a pharmacophore-based and ligand structure-based designing and screening strategy. The possible interaction of the residues with inhibitors suggests one binding-mode between the inhibitors and substrate binding pocket. Our modeling strategies and screening methods are appropriate for searching novel lead compounds having both structural diversity and high inhibitory activity against cy-FBP/SBPase. Our studies provide novel insight into the evolution of this enzyme family and may help in the design of inhibitors as algicide.

关键词： cyanobacteria FBP/SBPase interaction lead compound algicide

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Journal of Integrative Plant Biology 2006年第4期48卷 440-446页

作者： Jun-Feng Xu Ling jiang Xiang-Jin wei Wei-Wen Zhang Shi-Jia Liu Liang-Ming Chen Chun-Ming wang Lin-Guang Luo jian-Min wan State Key Laboratory of Crop Genetics and Germplasm Enhancement Research Center of Jiangsu Plant Gene Engineering Nanjing Agricultural University Nanjing 210095 China jiangxi Academy of Agricultural Science Nanchang 330200 China

II-32A, an elite male-sterile line of rice （Oryza sativa L.）, has been widely used for the production of hybrid rice seed In China. Heading date In most combinations using II-32A shows transgressive Inheritance or similarity to the latter parent, but the genotype of II-32A with respect to major genes for heading time Is unknown. This limits the further exploitation of this sterile line In breeding and hybrid seed production. Using a number of major gene heading date Isogenlc lines and heading date QTL near-lsogenic lines, we genetically analyzed II-32B under both long- and short-day conditions. We show that II-32B carries two photoperlod-sensltlve genes, E1 and E3, a recessive late-heading gene, ef-l, and a photoperlod-sensltlve allele, Se-1^u. In addition we Identified In II- 32B a recessive Inhibitor for E1 or Se-1^n and other modified photoperlod-sensltlve genes. The heading-date constitution of II-32A was determined to be E1e2E3Se-1^uef-li-Se-1.

关键词： heading date near-isogenic line photoperiod-sensitive gene recessive late-heading gene rice.

sPLA2-IB Level Correlates with Hyperlipidemia and the Prognosis of Idiopathic Membranous Nephropathy

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Current Medical Science 2020年第4期40卷 683-690页

作者： Li-yan YANG Yuan-sheng WU Bin-bin DAI Song-hua LIN Hong CHEN Guo-ping LI Xuan TAO jian-xin wan Yang-bin PAN Department of Nephrology the First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005China Shanghai Pudong Hospital Fudan University Pudong Medical CenterShanghai 201399China Department of Cardiology the First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005China Department of Pathology the First Affiliated Hospital of Fujian Medical UniversityFuzhou 350005China

Summary:Recent studies suggested that serum secretory phospholipase A2 group IB(sPLA2-IB)was increased in idiopathic membranous nephropathy(IMN).However,the interference of high lipemia on the sPL.A2-IB levels was not taken into account in these *** present study aimed to investigate the correlation between sPLA2-IB and lipemia,and the clinical merit of sPLA2-IB in the prediction of prognosis of IMN patients.A total of 64 IMN patients,39 immunoglobulin A nephropathy(IgAN)patients and 64 healthy controls were included in the *** levels of serum sPLA2-IB,lipemia and proteinuria were *** IMN patients were followed up for 6 *** stages were made for all IgAN and IMN *** results showed that the levels of serum sPLA2-IB,cholesterol and low-density lipoprotein cholesterol(LDL-C)were significantly higher,and the levels of albumin and high-density lipoprotein cholesterol(HDL-C)were significantly lower in IMN patients than in healthy controls and IgAN *** sPLA2-IB levels were also found to be higher in IgAN patients than in heathy controls,but the association of serum sPLA2-IB levels with proteinuria,cholesterol and albumin was only shown in IMN *** against M-type receptor for secretory phospholipase A2(PLA2R1)was positive in 813%IMN *** sPLA2-IB deposition,podocyte fused processes,and density deposition on thickened basement membrane were seen in IMN patients,but not in IgAN *** patients with lower sPLA2-IB and proteinuria levels were found to have better outcome after the 6-month *** IMN patients,sPLA2-IB levels were significantly increased in both serum and renal *** conclusion,serum sPLA2-IB was closely correlated with proteinuria,albumin and cholesterol,and IMN patients with lower sPLA2-IB levels were more likely to achieve a better outcome.

关键词： sPLA2-IB lipemia idiopathic membranous nephropathy prognosis

An Improved Distortion Operator for Insurance Risks

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Systems Science and Systems Engineering 2002年第3期11卷 296-301页

作者： GAO jian-wei, QIU wan-huaSchool of Economics & Management, Beijing University of Aeronautics and Astronautics, Beijing 100083, China School of Economics & Management Beijing University of Aeronautics and Astronautics Beijing 100083 China

This paper reviews the distortion function approach developed in the actuarial literature for insurance risks. The main aim of this paper is to derive an extensive distortion operator, and to propose a new premium principle based on this extensive distortion operator. Furthermore, the non-robustness of general distortion operator is also discussed. Examples are provided using Bernoulli, Pareto, Lognormal and Gamma distribution assumptions.

关键词： proportional hazards transform distortion operator non-robustness

Role of LOX3 Gene in Alleviating Adverse Effects of Drought and Pathogens in Rice

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Rice science 2008年第4期15卷 276-282页

作者： Nan-nan LIU[a] Ling jianG[a] Wen-wei ZHANG[a] Ling-long LIU[a] Hu-qu ZHAI[b] jian-min wan[a][b] [a] State Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural University/Jiangsu Plant Gene Engineering Research Center Nanjing 210095 China [b] Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China

Lipoxygenase 3 (LOX3) is a major component of the LOX isozymes in mature rice seeds. To investigate the role of LOX3 gene under stresses, a plant expression vector containing antisense cDNA of LOX3 was constructed. Rice varieties Wuyunjing 7 and Kasalath were transformed by the Agrobacterium-mediated method and transgenic rice plants were generated. PCR and Southern blot results showed that the antisense LOX3 gene was integrated into the rice genome. Analyses of embryo LOX3 deletion and semi-quantitative RT-PCR confirmed the antisense suppression of LOX3 gene in transgenic plants. The T2 antisense plants of LOX3 were sensitive to drought stress, rice blast and bacterial blight compared with non-transgenic plants. These results suggest that the LOX3 gene might function in response to stresses.

关键词： rice lipoxygenase gene antisense plant transgenic plants adverse stress drought stress rice blast bacterial blight gene function

Aurone derivatives as Vps34 inhibitors that modulate autophagy

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Acta Pharmaceutica Sinica B 2019年第3期9卷 537-544页

作者： Guodong Li Joshua William Boyle Chung-Nga Ko Wu Zeng Vincent Kam Wai Wong jian-Bo wan Philip Wai Hong Chan Dik-Lung Ma Chung-Hang Leung State Key Laboratory of Quality Research in Chinese Medicine Institute of Chinese Medical SciencesUniversity of MacaoMacao999078China School of Chemistry Monash UniversityClayton3800Australia Department of Chemistry Hong Kong Baptist UniversityHong Kong999077China State Key Laboratory of Quality Research in Chinese Medicine Macao University of Science and TechnologyMacao999078China Department of Chemistry University of WarwickCoventry CV47ALUK

We report in this study the identification of a natural product-like antagonist(1a) of Vps34 as a potent autophagy modulator via structure-based virtual screening. Aurone derivative 1a strongly inhibited Vps34 activity in cell-free and cell-based assays. Significantly, 1a prevents autophagy in human cells induced either by starvation or by an mT OR inhibitor. In silico modeling and kinetic data revealed that 1a could function as an ATP-competitive inhibitor of Vps34. Moreover, it suppressed autophagy in vivo and without inducing heart or liver damage in mice. 1a could be utilized as a new motif for more selective and efficacious antagonists of Vps34 for the potential treatment of autophagy-related human diseases.

关键词： Autophagy Natural products Vps34 Inhibitor Structure-based virtual screening Vesicle trafficking Heart or liver damage Aurone derivative

Laparoscopy for Crohn's disease:A comprehensive exploration of minimally invasive surgical techniques

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World Journal of Gastrointestinal Surgery 2021年第10期13卷 1190-1201页

作者： jian wan Chang Liu Xiao-Qi Yuan Mu-Qing Yang Xiao-Cai Wu Ren-Yuan Gao Lu Yin Chun-Qiu Chen Center for Difficult and Complicated Abdominal Surgery Shanghai Tenth People's HospitalTongji University School of MedicineShanghai 200072China Department of Medical Ultrasound Shanghai Tenth People’s HospitalUltrasound Research and Education InstituteTongji University School of MedicineShanghai 200072China

BACKGROUND Along with the unceasing progress of medicine,Crohn's disease(CD),especially complex CD,is no longer a taboo for minimally invasive ***,considering its special disease characteristics,more clinical trials are needed to confirm the safety and feasibility of laparoscopic surgery for *** To investigate the safety and feasibility of laparoscopic enterectomy for CD,assess the advantages of laparoscopy over laparotomy in patients with CD,and discuss comprehensive minimally invasive surgical techniques in complex *** This study prospectively collected clinical data from patients with CD who underwent enterectomy from January 2017 to January *** was registered in the Chinese clinical trial database with the registration number *** were divided into a laparoscopy group and a traditional laparotomy group according to the surgical *** baseline characteristics,operation time,intraoperative blood loss,temporary stoma,levels of abdominal adhesion,pathological characteristics,days to flatus and soft diet,postoperative complications,hospitalization time,readmission rate within 30 d,and hospitalization cost were compared between the two *** A total of 120 eligible patients were enrolled into the pre-standardized groups,including 100 in the laparoscopy group and 20 in the laparotomy *** with the laparotomy group,the postoperative hospitalization time in the laparoscopy group was shorter(9.1±3.9 d vs 11.0±1.6 d,P<0.05),the days to flatus were fewer(2.8±0.8 d vs 3.5±0.7 d,P<0.05),the days to soft diet were fewer(4.2±2.4 d vs 6.2±2.0 d,P<0.05)and the intraoperative blood loss was less(103.3±80.42 mL vs 169.5±100.42 mL,P<0.05).There were no statistically significant differences between the two groups in preoperative clinical data,operation time(149.0±43.8 min vs 159.2±40.0 min),stoma rate,levels of abdominal adhesion,total cost of hospitalization,incidence of postoperative complications[8.0%(8/100)

关键词： Crohn’s disease Minimally invasive surgery Rapid recovery Inflammatory bowel disease Ultrasound