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Macrophage-mimetic liposome adsorbing endotoxins for sepsis treatment

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Macrophage-mimetic liposome adsorbing endotoxins for sepsis ...

The Retrospective Diagnostic Potential of Gene Xpert MTB/RIF for the Analysis of Formalin-Fixed Paraffin-Embedded Tissue from Extrapulmonary Tuberculosis Patients

2018年第十二届中国药物制剂大会

作者：姜礼贤 Ruixiang Li jian’ge Zhang Innovation Research Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine

OBJECTIVE. Sepsis is a systemic inflammatory response syndrome mainly caused by bacterial infections. Severe sepsis may induce damage to organs and subsequent septic shock, which has become the leading cause of death in ICU patients worldwide. The common treatment for bacterial infections and sepsis is far from satisfaction. To develop a novel therapy for sepsis is urgently needed. Endotoxins, also refers to lipopolysaccharide（LPS）, have been regarded as the most important pathogenic factor in Gram-negative bacterial sepsis. Inspired by the intrinsic LPS-binding capacity of macrophage, a macrophage-mimetic liposome（M-Lipo） was developed for LPS neutralization. METhODS. The macrophage-mimetic liposome was designed under a hybrid concept. Firstly, macrophage-derived micro vesicles（MMV） were isolated from RAW264.7 cells by chemical stimulation, serving as membrane source. Then, the natural MMV was fused with artificial liposome by an extrusion method to prepare hybrid M-Lipo. Representative proteins of M-Lipo were detected using SDS-PAGE and Western blotting. The in vivo circulation of PEGylated M-Lipo was measured. The neutralization of LPS by M-Lipo was determined. And the therapeutic effect of M-Lipo was observed in LPS-infected cells. Finally, the survivals of sepsis-bearing mice after M-Lipo injection were observed. RESULTS. The result showed the M-Lipo had similar surface protein of macrophages, indicating MLipo could specifically bind LPS just like macrophages. As the pharmacokinetics experiment presented, MLipo showed 16% retention in blood and 382% more than the natural MMV at 12 h, indicating the combination of natural MMV and artificial lipid could integrate their advantages, which presumably could promote the elimination of LPS in vivo. The in vitro neutralization assay showed a neutralization capacity of 50 ng LPS per mg of M-Lipo. And M-Lipo significantly reduced LPS-induced inflammatory factor levels and i NOs levels. In a mouse LPS sensitive model, trea

关键词： Macrophage-mimetic liposome hybrid LPS sepsis

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Biomedical and Environmental Sciences 2023年第3期36卷 295-298页

作者： JIA Qing Jun ZENG Mei Chun ChENG Qing Lin h.ang Yin Yan WU Yi Fei LI Qing Chun Wang Le AI Li Yun Fang Zi jian ChENG Shi ShU Li Ping Department of TB Control and Prevention Hangzhou Center for Disease Control and PreventionHangzhou 310021ZhejiangChina Department of Pathology The First Affiliated HospitalCollege of MedicineZhejiang UniversityHangzhou 310003ZhejiangChina Department of Microbiological Examination Hangzhou Center for Disease Control and PreventionHangzhou 310021ZhejiangChina Department of Nutrition and Food Safety Hangzhou Center for Disease Control and PreventionHangzhou 310021ZhejiangChina

Tuberculosis(TB)is a chronic disease caused by infection with Mycobacterium *** confirmation of this infection is challenging due to the paucibacillary nature of extrapulmonary tuberculosis(EPTB).

关键词： MTB/RIF tuberculosis Tuberculosis

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基于结构模块化实现h.264编码软件

计算机科学 2005年第6期32卷 87-90页

作者：张剑谭劲华中科技大学计算机科学与技术学院

本文详细分析了两大标准化组织最新提出的视频编码国际标准h.264中,如何采用模块化、结构化方法来实现h.264编码软件,并对本实验室已完成的TMZJ2004软件,进行了速度和性能方面的测试,给出了编码器率失真性能曲线图。

关键词：率失真结构模块化 h.264 编码软件比特流编码视频流语法

固体汞合金电极钙黄绿素吸附催化伏安法测定轻稀土

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冶金分析 2008年第12期28卷 12-16页

作者：魏小平李建平桂林工学院材料与化学工程系

采用汞合金电极为工作电极,以铈为轻稀土代表,研究了在双氧水存在下,铈-钙黄绿素体系的吸附平行催化伏安法测定轻稀土。在ph 10.3的Nh3.h2O-Nh4Ac缓冲溶液中,铈-钙黄绿素络合物于-1.42 V可产生灵敏还原峰,加入双氧水后峰电流明显增大(... 详细信息

采用汞合金电极为工作电极,以铈为轻稀土代表,研究了在双氧水存在下,铈-钙黄绿素体系的吸附平行催化伏安法测定轻稀土。在ph 10.3的Nh3.h2O-Nh4Ac缓冲溶液中,铈-钙黄绿素络合物于-1.42 V可产生灵敏还原峰,加入双氧水后峰电流明显增大(约20倍)。导数峰高与Ce(Ⅳ)浓度在0.02~5μg/mL范围内呈线性关系,方法检出限为0.01μg/mL。研究了电流性质及电极反应机理,证明其为络合物吸附催化波。铈与钙黄绿素形成络合比为1∶1的络合物,络合物中的钙黄绿素在电极上还原,电极上还原的中间产物被双氧水氧化成原价态,继续参加电极反应,从而形成一种催化循环,使峰电流增大。利用固体汞合金为电极,钙黄绿素络合吸附催化波法可测定稀土球墨铸铁中轻稀土总量,结果同认定值相吻合。

关键词：吸附催化伏安法轻稀土固体汞合金电极钙黄绿素

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电弧喷涂铝基涂层耐高温硫化腐蚀性能的研究

金属热处理 2007年第12期32卷 53-56页

作者：任春岭魏琪栗卓新方建筠周新建北京工业大学材料科学与工程学院北京100022

对电弧喷涂铝基涂层在600℃条件下的耐硫化氢腐蚀性能进行了研究,并与Fe-Al涂层和A304不锈钢的耐h2S腐蚀性能进行了对比。结果表明,铝基涂层的耐硫化氢腐蚀性能优于Fe-Al涂层和A304不锈钢。铝基涂层具有良好的耐h2S腐蚀性能的主要原因... 详细信息

对电弧喷涂铝基涂层在600℃条件下的耐硫化氢腐蚀性能进行了研究,并与Fe-Al涂层和A304不锈钢的耐h2S腐蚀性能进行了对比。结果表明,铝基涂层的耐硫化氢腐蚀性能优于Fe-Al涂层和A304不锈钢。铝基涂层具有良好的耐h2S腐蚀性能的主要原因是涂层中的Al含量很高,在腐蚀过程中能优先生成Al的腐蚀产物以及涂层中含有多种铝的金属间化合物,能够阻碍硫原子向金属内部渗入,从而保证了铝基涂层具有良好的耐硫化腐蚀性能。

关键词：电弧喷涂粉芯线材铝基涂层高温硫化腐蚀

Aminoalkylaion of Benzothiazoles and Activated Alkenes via Cross-Dehydrogenative-Coupling

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The effect of BACE1 in pressure culture damage for human trabecular meshwork cell

中国化学会第九届全国有机化学学术会议

作者： jian Wang Jing Li Jinbo h.ang Qiang Zhu Guangzhou Institutes of Biomedicine and health Chinese Academy of Sciences

In recent years,direct functionalization of inert C-h bonds has attracted fundamental interests of organic chemists due to the atom-economic nature of this strategy.

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The effect of BACE1 in pressure culture damage for human tra...

中国神经科学学会第十届全国学术会议

作者： jian XIang Lan LI Jia LUO hui Wang Department of Anatomy and Neurobiology Xiangya School of MedicineCentral South University

Objectives BACEl（β-site APP cleaving enzyme）,mediates a critical step in the production of Aβ40 and *** is a presumed to be involved in the pathobiology of various neurodegenerative diseases,including *** human trabecular meshwork cell play a key role in glaucoma,it is believed that take part in the regulation of homeostasis of aqueous *** has shown that in some glaucoma patient aqueous humor is full of *** investigated the high Pressure cell culture model of human trabecular meshwork cells in vitro,observe effects of Pressure on cultured htmc and bacel expression in bacel,and effects of bacel expression on cultured human trabecular meshwork cell（hTMC）.Methods we used special equipment exert pressure for human trabecular meshwork cell（20 mmhg,40 mmhg,60 mmhg,80 mmhg,pressure lhour,after pressure,Normal Condition live 12 h,24 h,48 h）,hTMC functional changes levels,BACE1 expression changes were quantified by immunohistochemistry and western ***（1） Under the different pressure to cultivate,There is an obvious change of BACE1 expression,is increased.（2） As the pressure rises,the damage situation of trabecular cells is increased.（3） Under the same pressure,Injecting the agonist of bacel,the damage situation of human trabecular meshwork cells is increased,injecting the inhibitor,the damage situation of human trabecular meshwork cells is *** BACE1 was produced in pressure condition culture and damage for human trabecular meshwork cell.

关键词： BACE1 hTMC pressure

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风雨操场钢屋盖滑移施工关键技术

建筑机械化 2020年第10期41卷 64-67页

作者：邹建磊李昂范锐钊赵国强北京市机械施工有限公司北京100045

中国传媒大学风雨操场钢屋盖为立体桁架结构,鉴于施工场地条件限制,该工程采用累积滑移施工方案,介绍了累积滑移技术的总体思路、施工流程、滑移装置的配置等。通过仿真计算分析,保证了桁架、标准节和滑移设施的受力和变形满足要求,供... 详细信息

中国传媒大学风雨操场钢屋盖为立体桁架结构,鉴于施工场地条件限制,该工程采用累积滑移施工方案,介绍了累积滑移技术的总体思路、施工流程、滑移装置的配置等。通过仿真计算分析,保证了桁架、标准节和滑移设施的受力和变形满足要求,供今后类似工程参考。

关键词：钢结构立体桁架屋盖累积滑移仿真分析

皮肤磨削术联合光动力疗法治疗鲍恩病疗效观察

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临床皮肤科杂志 2014年第12期43卷 743-745页

作者：羊剑秋江阴市中医院皮肤科江苏江阴214400

目的:观察皮肤磨削术联合光动力疗法(PDT)治疗鲍恩病的临床疗效。方法:将门诊经组织病理诊断为鲍恩病的19例患者随机分为两组。联合治疗组:先进行肿瘤皮损的皮肤磨削术,术后外用5-氨基酮戊酸(ALA)溶液封包,5 h后给予红光照射,波长635 nm... 详细信息

目的:观察皮肤磨削术联合光动力疗法(PDT)治疗鲍恩病的临床疗效。方法:将门诊经组织病理诊断为鲍恩病的19例患者随机分为两组。联合治疗组:先进行肿瘤皮损的皮肤磨削术,术后外用5-氨基酮戊酸(ALA)溶液封包,5 h后给予红光照射,波长635 nm,能量密度为100 J/cm^2,照射20 min。14 d后待创面愈合进行第2次,1周后进行第3次,3次为一个疗程,于1、2、3、6周及半年观察患者病灶局部反应。对照组:单用PDT,方法同联合治疗组,每周1次,3次为一个疗程。结果:联合治疗组治疗后,8例患者病灶完全缓解,2例患者病灶明显缩小>50%。对照组中5例完全缓解,3例部分缓解,1例无效。两组完全缓解率差异有统计学意义。结论:皮肤磨削术结合PDT可以作为鲍恩病治疗的一种新选择。

关键词：鲍恩病磨削术光动力疗法