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Determination of Cordycepin in Cordyceps kyushuensis by Capillary Electrophoresis and its Antitumour Activity

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Chinese Chemical Letters 2003年第7期14卷 724-727页

作者： Ying Jie SUN jian ya ling Peng L Han Xing ZHANG Chang Kai ZHANG State Key Laboratory of Microbial Technology Life Science School of Shandong UniversityJinan 250100

A simple, rapid and low-cost method of determination for cordycepin in Cordyceps kyushuensis by capillary zone electrophoresis (CZE) was developed. Based on the finding that there is a high concentration of cordycepin in both natural and cultured Cordyceps kyushuensis, the in vitro antitumor activity of cordycepin and the water extracts of Cordyceps kyushuensis has been investigated. This is the first report about the antitumor effect of Cordyceps kyushuensis.

关键词： Cordycepin Cordyceps kyushuensis capillary zone electrophoresis cancer cell line antitumor activity.

Systematic profiling of SARS-CoV-2-specific IgG epitopes at amino acid resolution

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Cellular & Molecular Immunology 2021年第4期18卷 1067-1069页

作者： Huan Qi Ming-liang Ma He-wei jiang jian-ya ling ling-yun Chen Hai-nan Zhang Dan-yun Lai yang Li Zi-wen Guo Chuan-sheng Hu Shu-juan Guo Qing-feng Meng yan Ren Xiao yang Wei Wang Jie Zhou Xiao-dong Zhao Hua Li Sheng-ce Tao Shanghai Center for Systems Biomedicine Key Laboratory of Systems Biomedicine(Ministry of Education)Shanghai Jiao Tong UniversityShanghaiChina State Key Laboratory of Microbial Technology Shandong UniversityQingdaoShandongChina BGI Education Center University of Chinese Academy of SciencesShenzhenGuangdongChina State Key laboratory for Oncogenes and Bio-ID Center School of Biomedical EngineeringShanghai Jiao Tong UniversityShanghaiChina BGI-Shenzhen ShenzhenGuangdongChina Foshan Fourth People's Hospital FoshanGuangdongChina Institute of Biophysics Chinese Academy of SciencesBeijingChina

There is a worldwide pandemic of COVID-19 caused by SARS-CoV-2.1 By January 28,2021,more than 100 million cases had been diagnosed,and more than 2 million deaths had been reported(https://***/***).The comprehensive and in-depth elucidation of SARS-CoV-2-specific IgG responses will help us to better understand COVID-19 immunity and facilitate the precise development of neutralizing antibodies and vaccines.

关键词： map. precise neutral

Long-term efficacy and safety of drug-eluting stent implantation for patients with multiple coronary chronic total occlusions

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Chinese Medical Journal 2010年第7期123卷 789-793页

作者： ZHANG jian HAN ya-ling LI Yi WANG Shou-li JING Quan-min WANG Xiao-zeng MA Ying-yan LUAN Bo WANG Geng WANG Bin Department of Cardiology Shenyang Northern HospitalShenyang Liaoning 110016 China

Background Data on the efficacy and safety of drug-eluting stent （DES） for treatment of multiple coronary chronic total occlusion （CTO） lesions are scanty. The aim of the present study was to compare the long-term outcomes of DES versus bare metal stent （BMS） implantation for multiple coronary CTO *** We analyzed 188 patients who underwent coronary stenting for at least two de novo CTO lesions in our center from November 2000 to November 2006. Among them, 118 patients （62.8%） received DES and 70 patients （37.2%） received BMS implantation after the recanalization for CTO lesions. All patients were followed up for up to 5 years for the occurrence of major adverse cardiac events （MACE）. Long-term survival rates were estimated with the Kaplan-Meier *** There were no significant differences in baseline clinical characteristics and procedural success rate between DES group and BMS group. Compared with the BMS group, the DES group showed a significantly higher rate of long CTO （≥15 mm）（62.0% vs. 50.6%, P=0.023）. The number of stents per lesion （1.39±0.71 vs. 1.17±0.66, P=0.007） and the mean length of stents in the DES group were also higher than those in the BMS group （（40.8±11.4） mm vs. （23.4±8.7） mm, P〈0.001）. But the mean diameter of stents in the DES group was smaller than that in the BMS group （（3.1 ±0.2） mm vs. （3.3±0.5） mm, P〈0.001）. Average follow-up time was 4.8±0.7 （1.5-5.0） years in the BMS group and 4.3±0.5 （1.3-5.0） years in the DES group. Both the 5-year cumulative survival rates and the target vessel revascularization （TVR）-free survival rates of the DES group were significantly higher than those in the BMS group （83.1% vs. 72.9%, Log-rank P=0.044; 77.1% vs. 62.9%, Log-rank P=0.009）. The cumulative MACE-free survival rates in the DES group were significantly higher than those in the BMS group （71.2% vs. 51.4%, Log-rank P=0.001）. Multivariable Cox regression analysis demonstrated that DES implantation for multiple CTO lesions could sig

关键词： chronic disease coronary disease angioplasty, transluminal, percutaneous coronary stents

Association of the ALOX5AP haplotypes with susceptibility to coronary artery disease in Chinese Han population

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Association of the ALOX5AP haplotypes with susceptibility to...

Comparative proteomic analysis of plasma exosomes reveals the functional contribution of N-acetyl-alpha-glucosaminidase to Parkinson’s disease

第十三次全国心血管病学术会议

作者： ZHANG Xiao-lin,LI Zhi-dong,yaN Cheng-hui,KANG jian.HAN ya-ling Department of Cardiology.Cardiovascular Research Institute, Northern Hospital,Shenyang 110016,China

Objectives To investigate whether the ALOX5AP polymorphisms is associated to coronary artery disease(CAD) in a sample of Chinese Han *** We enrolled 48 unrelated individuals of North China including 24 CAD patients confirmed by selective coronary arteriography(SCA) and 24 healthy subjects for the single nucleotide polymorphisms(SNP) screening.A total of 656 patients with CAD and 678 control subjects were included in the *** and controls were unrelated individuals of Han Chinese from the northeast region of *** the subjects had undergone standardized coro-

关键词：

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Neural Regeneration Research 2025年第10期20卷 2998-3012页

作者： Yuan Zhao Yidan Zhang Xin Liu jian Zhang ya Gao Shuyue Li Cui Chang Xiang Liu Guofeng yang Department of Geriatrics Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina Department of Neurosurgery The Second Hospital of Hebei Medical UniversityShijiazhuangHebei ProvinceChina

Parkinson’s disease is the second most common progressive neurodegenerative disorder,and few reliable biomarkers are available to track disease *** proteins,DNA,mRNA,and lipids carried by exosomes reflect intracellular changes,and thus can serve as biomarkers for a variety of *** this study,we investigated alterations in the protein content of plasma exosomes derived from patients with Parkinson’s disease and the potential therapeutic roles of these proteins in Parkinson’s *** a tandem mass tag-based quantitative proteomics approach,we characterized the proteomes of plasma exosomes derived from individual patients,identified exosomal protein signatures specific to patients with Parkinson’s disease,and identified N-acetyl-alpha-glucosaminidase as a differentially expressed protein.N-acetyl-alpha-glucosaminidase expression levels in exosomes from the plasma of patients and healthy controls were validated by enzyme-linked immunosorbent assay and western *** results demonstrated that the exosomal N-acetyl-alpha-glucosaminidase concentration was not only lower in Parkinson’s disease,but also decreased with increasing Hoehn-yahr stage,suggesting that N-acetyl-alpha-glucosaminidase could be used to rapidly evaluate Parkinson’s disease ***,western blot and immunohistochemistry analysis showed that N-acetyl-alpha-glucosaminidase levels were markedly reduced both in cells treated with 1-methyl-4-phenylpyridinium and cells overexpressingα-synuclein compared with control ***,N-acetyl-alpha-glucosaminidase overexpression significantly increased cell viability and inhibitedα-synuclein expression in 1-methyl-4-phenylpyridinium-treated *** together,our findings demonstrate for the first time that exosomal N-acetyl-alpha-glucosaminidase may serve as a biomarker for Parkinson’s disease diagnosis,and that N-acetyl-alpha-glucosaminidase may reduceα-synuclein expression and 1-methyl-4-phenylpyridinium-induced n

关键词： biomarker diagnosis exosomes N-acetyl-alpha-glucosaminidase Parkinson’s disease proteomic α-synuclein

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非平衡样本下变电站变压器运行扰动诊断研究

制造业自动化 2021年第12期43卷 174-177页

作者：周萍周英蹇亚玲杨博宇四川省电力公司经济技术研究院成都610000 国网四川信通公司成都610000

考虑到当前变压器运行扰动诊断方法对未标识数据的分析能力较差,导致变压器运行扰动诊断正判率较低的问题,设计非平衡样本下变电站变压器运行扰动诊断方法。计算综合最优映射目标函数,应用非平衡样本提取变压器运行扰动特征。根据扰动... 详细信息

考虑到当前变压器运行扰动诊断方法对未标识数据的分析能力较差,导致变压器运行扰动诊断正判率较低的问题,设计非平衡样本下变电站变压器运行扰动诊断方法。计算综合最优映射目标函数,应用非平衡样本提取变压器运行扰动特征。根据扰动特征结合Dempster规则,构建变压器运行扰动多特征证据体系。使用万能逼近定理,搭建运行扰动诊断网络,实现变压器运行扰动诊断。至此,非平衡样本下变电站变压器运行扰动诊断方法设计完成。构建仿真实验环节,实验结果表明:此方法在非平衡样本条件下,可获取正判率较高的单一扰动与复合扰动诊断结果。

关键词：变电站变压器状态评估数据驱动特征提取非平衡样本扰动诊断

基于相对鲁棒CVaR的电网投资项目组合优化模型研究

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数学的实践与认识 2019年第9期49卷 42-52页

作者：杜英苟全峰杨杰何璞玉骞亚玲杨佳澄国网四川省电力公司经济技术研究院四川成都610000 华北电力大学经济与管理学院北京102206

在新一轮电改的背景下,电网投资将面临更多的不确定性风险,亟需落实精准投资以降低投资风险.将相对鲁棒CVaR风险度量模型应用于电网投资项目组合优化中,构建了基于相对鲁棒CVaR的电网投资项目组合优化模型,并通过蒙特卡洛仿真和K-means... 详细信息

在新一轮电改的背景下,电网投资将面临更多的不确定性风险,亟需落实精准投资以降低投资风险.将相对鲁棒CVaR风险度量模型应用于电网投资项目组合优化中,构建了基于相对鲁棒CVaR的电网投资项目组合优化模型,并通过蒙特卡洛仿真和K-means聚类方法进行随机样本的生成与削减.算例结果表明,相对鲁棒CVaR模型具有极好的鲁棒性,能够在相对最坏情景下保证电网投资风险的最小化;同时,相对于绝对鲁棒CVaR模型减小了决策结果的保守性.

关键词：电网投资不确定性 WCVaR RRCVaR

3-氯-4-二氯甲基-5-羟基-2(5氢)-呋喃酮对L-02细胞DNA损伤与ras基因表达的影响

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卫生毒理学杂志 2004年第4期18卷 207-209页

作者：刘爱林吴建军邹亚玲鲁文清华中科技大学同济医学院公共卫生学院劳动卫生与环境卫生学系武汉430030

目的　研究饮水氯化消毒副产物 3 氯 4 二氯甲基 5 羟基 2 (5氢 ) 呋喃酮 (MX)对人胚肝细胞 (L 0 2 )DNA损伤与ras基因表达的诱导 ,初步探讨MX致癌的分子机制。方法　以L 0 2细胞作为靶细胞 ,应用彗星试验 (SCGE)和原位杂交试验 (... 详细信息

目的　研究饮水氯化消毒副产物 3 氯 4 二氯甲基 5 羟基 2 (5氢 ) 呋喃酮 (MX)对人胚肝细胞 (L 0 2 )DNA损伤与ras基因表达的诱导 ,初步探讨MX致癌的分子机制。方法　以L 0 2细胞作为靶细胞 ,应用彗星试验 (SCGE)和原位杂交试验 (ISH)研究MX诱导L 0 2细胞DNA损伤与ras基因表达。MX设 10、3 0、10 0和 3 0 0 μmol L 4个剂量 ,二甲基亚砜 (DMSO ,10mL L)为溶剂对照 ,H2 O2 (3 0 0 μmol L)和B(a)P(2 0 0 μmol L)分别为SCGE和ISH的阳性对照。结果　与溶剂对照相比 ,3 0、10 0和 3 0 0 μmol L的MX能明显增加L 0 2细胞的DNA迁移度 (P <0 0 5 ,P <0 0 1,P <0 0 0 1) ;同时也明显增加ras基因表达水平 (P <0 0 0 1,P <0 0 0 1,P <0 0 1)。MX在 10～ 10 0 μmol L的浓度范围内 ,诱导的ras基因表达水平和DNA迁移度呈正相关 (r =0 79)。结论　饮水氯化消毒副产物MX可诱导L 0 2细胞DNA损伤与ras基因表达 ,ras基因表达水平可能与DNA损伤程度有关。

关键词： ras基因表达水平诱导 DNA损伤细胞DNA SCGE 对照呋喃酮氯甲基溶剂

TBL、PBL与CBL整合教学法在口腔修复学本科生实习教学中的应用

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大连医科大学学报 2016年第5期38卷 483-486页

作者：何建亚王君玲李晓杰胡书海大连医科大学口腔医学院辽宁大连116044

目的探讨将分组教学法(team-based learning,TBL)、问题教学法(problem-based learning,PBL)、案例教学法(case-based learning,CBL)整合教学法,应用到口腔修复学本科生实习教学中所取得的效果。方法选取大连医科大学2011级口腔医学专... 详细信息

目的探讨将分组教学法(team-based learning,TBL)、问题教学法(problem-based learning,PBL)、案例教学法(case-based learning,CBL)整合教学法,应用到口腔修复学本科生实习教学中所取得的效果。方法选取大连医科大学2011级口腔医学专业本科实习生30人,随机分成两组(n=15)。实验组采用TBL、PBL、CBL整合教学法,对照组采用传统教学方法。实习结束后通过调查问卷、病例分析和基础知识考试成绩评价该教学法对学生临床能力的教学效果。结果实验组对教学模式的满意度达88.0%,显著高于传统教学组75.3%,P0.05。结论 TBL、PBL、CBL整合教学法能提高口腔修复学临床实习的质量,实习生有更高的满意度。

关键词： TBL PBL CBL 口腔修复学临床实习