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Rapid detection of intestinal pathogens in fecal samples by an improved reverse dot blot method

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World Journal of Gastroenterology 2009年第20期15卷 2537-2542页

作者： jian-Ming xing Su Zhang Ying Du Dan Bi Li-Hui Yao Huzhou Maternity and Child Care Hospital Huzhou 313000 Zheiiang Province China

AIM:To develop a new, rapid and accurate reverse dot blot(RDB) method for the detection of intestinal pathogens in fecal ***:The 12 intestinal pathogens tested were Salmonella spp., Brucella spp., Escherichia coli O157:H7, Clostridium botulinum , Bacillus cereus , Clostridium perfringens , Vibrio parahaemolyticus , Shigella spp., Yersinia enterocolitica, Vibrio cholerae, Listeria monocytogenes and Staphylococcus *** two universal primers were designed to amplify two variable regions of bacterial 16S and 23S rDNA genes from all of the 12 bacterial species *** hundred and forty fecal samples from the diarrhea patients were detected using the improved RDB ***:The methods could identify the 12 intestinal pathogens specifi cally, and the detection limit was as low as 103 *** consistent detection rate of the improved RDB assay compared with the traditional culture method was up to 88.75%.CONCLUSION:The hybridization results indicated that the improved RDB assay developed was a reliable method for the detection of intestinal pathogen in fecal samples.

关键词： Immunoblotting Intestinal pathogens Feces

Optimizing ef?ciency of polycrystalline p-Si anode organic light-emitting diode

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Rare Metals 2016年第11期35卷 826-830页

作者： jian-xing Luo Wei Wang Hu Meng Wan-Jin Xu Guo-Gang Qin State Key Laboratory for Mesoscopic Physics School of Physics Peking University Beijing 100871 China Key Laboratory of Semiconductor Materials Chinese Academyof Science Beijing 100083 China

Optimizing efficiency of organic light-emitting diodes（OLEDs） with a structure of Al/glass/nanometerthick polycrystalline p-Si（NPPS） anode/SiO_2/N＇-bis-（1-naphthl）-diphenyl-1,1＇-biphenyl-4,4＇-diamine（NPB）/tris（8-hydroxyquinoline） aluminum（Alq_3）/4,7-diphenyl^（-1）,10-phenanthroline（BPhen）：Cs_2CO_3/Sm/Au were studied. The NPPS anodes were fabricated by magnetron sputtering（MS）Si and Ni layers followed by Ni-induced crystallization of the amorphous Si layers. By adjusting the resistivity of the p-Si target adopted in MS, the electroluminescent efficiency of the OLED was optimized. When the resistivity of the p-Si target is 0.01 Ω·cm, the current and power efficiency of the NPPS anode OLED reach maximum values of 6.7 cd ·A^（-1）and 4.64 lm ·W^（-1）, respectively, which are 2.7 and 3.1 times those of the resistivity-optimized bulk p-Si anode counterpart and 2.9 and 3.7 times those of the indium tin oxide（ITO） anode counterpart, and then, the physical reasons were discussed.

关键词： Organic light-emitting diode Polycrystallinep-Si Magnetron sputtering Electroluminescence

A novel method of quantitative evaluation and comprehensive classification of low permeability-tight oil reservoirs: A case study of Jidong Oilfield, China

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Petroleum Science 2022年第4期19卷 1527-1541页

作者： Dong-Liang jiang Hao Chen jian-Peng xing Lin Shang Qun-Hui Wang Yan-Chun Sun Yao Zhao jian Cui Ian Duncan State Key Laboratory of Petroleum Resources and Prospecting China University of Petroleum(Beijing)Beijing102249China Key Lab of Petroleum Eng.of MOE China University of Petroleum(Beijing)Beijing102249China Postdoctoral Station of PetroChina Jidong Oilfield Company TangshanHebei 063000China Postdoctoral Program of PetroChina Research Institute of Petroleum Exploration and Development Beijing100083China Exploration and Development Research Institute PetroChina Jidong Oilfield CompanyTangshanHebei063000China Bureau of Economic Geology The University of Texas at Austin78705United States

The classification of low permeability-tight reservoirs is the premise of development. The deep reservoir of Shahejie 3 member contains rich low permeability-tight reserves, but the strong heterogeneity and complex micro pore structure make the main controlling factors subjective and the classification boundaries unclear. Therefore, a new indicator considering the interaction between fluid and rock named Threshold Flow Zone Indicator(TFZI) is proposed, it can be used as the main sequence of correlation analysis to screen the main controlling factors, and the clustering algorithm is optimized combined with probability distribution to determine the classification boundaries. The sorting coefficient, main throat radius, movable fluid saturation and displacement pressure are screened as the representative parameters for the following four key aspects: rock composition, microstructure, flow capacity and the interaction between rock and fluid. Compared with the traditional probability distribution and clustering algorithm, the boundary of the optimized clustering algorithm proposed in this paper is more *** classification results are consistent with sedimentary facies, oil levels and oil production *** method provides an important basis for the development of low permeability-tight reservoirs.

关键词： Low permeability-tight reservoir Classification boundary Correlation analysis Probability distribution Clustering algorithm

Interface Analysis and Hot Deformation Behaviour of a Novel Laminated Composite with High-Cr Cast Iron and Low Carbon Steel Prepared by Hot Compression Bonding

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Journal of Iron and Steel Research International 2015年第5期22卷 438-445页

作者： Zheng-yi jianG xing-jian GAO Sheng-li LI Hong-mei ZHANG Deng-fu CHEN jian-zhong XU School of Materials and Metallurgy University of Science and Technology Liaoning School of Mechanical Materials and Mechatronic EngineeringUniversity of Wollongong College of Materials Science and Engineering Chongqing University State Key Laboratory of Rolling and Automation Northeastern University

A hot compression bonding process was developed to prepare a novel laminated composite consisting of high-Cr cast iron （HCCI） as the inner layer and low carbon steel （LCS） as the outer layers on a Gleeble 3500 ther- momechanicat simulator at a temperature of 950 ℃ and a strain rate of 0. 001 s 1. Interfacial bond quality and hot deformation behaviour of the laminate were studied by mierostructural characterisation and mechanical tests. Experi- mental results show that the metallurgical bond between the constituent metals was achieved under the proposed bonding conditions without discernible defects and the formation of interlayer or intermetallic layer along the inter- face. The interfacial bond quality is excellent since no deterioration occurred around the interface which was deformed by Vickers indentation and compression test at room temperature with parallel loading to the interface. After well cladding by the LCS, the brittle HCCI can be severely deformed （about 57 % of reduction） at high temperature with crack-free. This significant improvement should be attributed to the decrease of crack sensitivity due to stress relief by soft claddings and enhanced flow property of the HCCI by simultaneous deformation with the LCS.

关键词： bonding laminated metal composite interface structure hot working high-Cr cast iron

Enhanced saccharification of lignocellulosic biomass with1-allyl-3-methylimidazolium chloride(Amim Cl) pretreatment

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Chinese Chemical Letters 2014年第11期25卷 1485-1488页

作者： jian-Fei Liu Yan Cao Mao-Hua Yang Xue-Jing Wang Hui-Quan Li jian-Min xing National Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences Key Laboratory of Green Process Engineering Institute of Process Engineering Chinese Academy of Sciences

A simple and efficient method of enhancing biomass saccharification by microwave-assisted pretreatment with dimethyl sulfoxide/1-allyl-3-methylimidazolium chloride is proposed. Softwood（pine wood（PW））, hardwoods（poplar wood, catalpa bungi, and Chinese parasol）, and agricultural wastes（rice straw, wheat straw, and corn stover（CS）） were exploited. Results showed that the best pretreatment effect was in PW with 54.3% and 31.7% dissolution and extraction ratios, respectively. The crystal form of cellulose in PW extract transformed from I to II, and the contended cellulose ratio and glucose conversion ratio reached 85.1% and 85.4%, respectively. CS after steam explosion achieved a similar pretreating effect as PW, with its cellulose hydrolysis ratio reaching as high as 91.5% after IL pretreatment.

关键词： Ionic liquid Lignocellulosic biomass Pretreatment Saccharification

Quantum Teleportation of One-to-Many Using (n+1)-Particle Entanglement

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Communications in Theoretical Physics 2007年第10期48卷 623-625页

作者： SHA Jin-Qiao FANG jian-xing jianG Wei-xing School of Physical Science and Technology,Suzhou University,Suzhou 215006,China School of Physical Science and Technology Suzhou University

In this paper,quantum teleportation of one-to-many using(n+1)-particle entanglement is *** thesender(Alice)wants to transmit an unknown quantum state to a distant receiver(Bob),similar to the previous schemes,Alice performs Bell-state measurement on particles belonging to herself and informs the receiver the results through theclassical *** that,it needs to perform the Hadamard operation on the other(n-1)particles and measurethem as *** the aid of the measurement results,Bob can operate a corresponding unitary transformation on hisparticle to reconstruct the original *** course,the reconstruction may realize at either location of n,but it cannotrealize at all locations at the same time.

关键词： quantum teleportation quantum entangled state unitary transformation

Shadow obstacle model for realistic corner-turning behavior in crowd simulation

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Frontiers of Information Technology & Electronic Engineering 2016年第3期17卷 200-211页

作者： Gao-qi HE Yi JIN Qi CHEN Zhen LIU Wen-hui YUE xing-jian LU Department of Computer Science and Engineering East China University of Science and Technology Shanghai 200237 China MOE Key Laboratory of Geographic Information Science East China Normal University Shanghai 200241 China State Key Laboratory of Virtual Reality Technology and Systems Beihang University Beijing 100191 China College of Information Science and Technology Ningbo University Ningbo 315211 China

This paper describes a novel model known as the shadow obstacle model to generate a realistic comer-tuming behavior in crowd simulation. The motivation for this model comes from the observation that people tend to choose a safer route rather than a shorter one when turning a comer. To calculate a safer route, an optimization method is proposed to generate the corner-turning rule that maximizes the viewing range for the agents. By combining psychological and physical forces together, a full crowd simulation framework is established to provide a more realistic crowd simulation. We demonstrate that our model produces a more realistic comer-turning behavior by comparison with real data obtained from the experiments. Finally, we per- form parameter analysis to show the believability of our model through a series of experiments.

关键词： Comer-turning behavior Crowd simulation Safety awareness Rule-based model

Quantum Logic Networks for Probabilistic Teleportation of an Arbitrary Three-Particle State

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Communications in Theoretical Physics 2005年第4X期44卷 611-614页

作者： QIAN Xue-Mir FANG jian-xing ZHU Shi-Qun XI Yong-Jun School of Physical Science and Technology Suzhou University Suzhou 215006 China

The scheme for probabilistic teleportation of an arbitrary three-particle state is proposed. By using single qubit gate and three two-qubit gates, efficient quantum logic networks for probabilistic teleportation of an... 详细信息

关键词： probabilistic teleportation arbitrary three-particle state quantum logic networks

A comparative study of combustible cartridge case materials

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Defence Technology（防务技术） 2017年第3期13卷 127-130页

作者： Wei-tao Yang jian-xing Yang Yu-cheng Zhang San-jiu Ying Xi'an Modern Chemistry Research Institute School of Chemical Engineering Nanjing University of Science and Technology

Foamed combustible material based on polymer bonded RDX was fabricated using CO_2 as foaming *** inner structures of felted and foamed combustible materials were presented by SEM. The two materials presented different formulations and inner porous structures. The combustion behaviors of felted and foamed materials were investigated by closed vessel test. Simultaneously, the co-combustion behavior of combustible cartridge case with 7-perf consolidated propellants was also investigated. The results of closed vessel test is applicable to gun system which is made of the foamed combustible material as component.

关键词：可燃材料可燃药筒密闭容器粘结炸药内部结构多孔结构发泡材料燃烧行为