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重度发热伴血小板减少综合征脑炎一例

中国现代神经疾病杂志 2024年第4期24卷 253-257页

作者：徐金梅刘兵舰关鸿志单萍陈国华梅俊华湖北省武汉市第一医院神经内科 430022 中国医学科学院北京协和医学院北京协和医院神经科100730

患者女性,65岁,农民。主因间断性发热伴腹泻、尿频6天,近事记忆减退伴言语不能1天,于2022年5月7日收入湖北省武汉市第一医院。患者入院前10天(4月29日)被克氏原螯虾(又称小龙虾)夹破右手示指,未予重视;入院前5天(5月2日)无明显诱因出现... 详细信息

患者女性,65岁,农民。主因间断性发热伴腹泻、尿频6天,近事记忆减退伴言语不能1天,于2022年5月7日收入湖北省武汉市第一医院。患者入院前10天(4月29日)被克氏原螯虾(又称小龙虾)夹破右手示指,未予重视;入院前5天(5月2日)无明显诱因出现腹泻,呈黄色水样便(5~6次/d),尿频(每5分钟1次,每次约20 ml),伴头晕、后颈部胀痛、胸闷,间断发热,体温最高38.1℃。

关键词：布尼亚病毒科感染重度发热伴血小板减少综合征脑炎宏基因组学基因检测病例报告

The collagen type Ⅰ alpha 1 chain gene is an alternative safe harbor locus in the porcine genome

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Journal of Integrative Agriculture 2023年第1期22卷 202-213页

作者： XIANG Guang-ming ZHANG Xiu-ling XU Chang-jiang FAN Zi-yao XU kui WANG Nan WANG Yue CHE Jing-jing XU Song-song MU Yu-lian LI kui liu Zhi-guo State Key Laboratory of Animal Nutrition/Key Laboratory of Animal Genetics Breeding and Reproduction of Ministry of Agriculture and Rural Affairs of China Institute of Animal SciencesChinese Academy of Agricultural SciencesBeijing 100193P.R.China School of Life Science and Engineering Foshan UniversityFoshan 528231P.R.China Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs Agricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518120P.R.China

Efficient and stable expression of foreign genes in cells and transgenic animals is important for gain-of-function studies and the establishment of *** harbor loci in the animal genome enable consistent overexpression of foreign genes,without side ***,relatively few safe harbor loci are available in pigs,a fact which has impeded the development of multi-transgenic pig *** report a strategy for efficient transgene knock-in in the endogenous collagen type I alpha 1 chain(COL1A1)gene using the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9(CRISPR/Cas9)*** the knock-in of a 2A peptide-green fluorescence protein(2A-GFP)transgene in the last codon of COL1A1 in multiple porcine cells,including porcine kidney epithelial(PK15),porcine embryonic fibroblast(PEF)and porcine intestinal epithelial(IPI-2I)cells,quantitative PCR(qPCR),Western blotting,RNA-seq and CCK8 assay were performed to assess the safety of COL1A1 *** qPCR results showed that the GFP knock-in had no effect(P=0.29,P=0.66 and P=0.20 for PK15,PEF and IPI-2I cells,respectively)on the mRNA expression of COL1A1 ***,no significant differences(P=0.64,P=0.48 and P=0.80 for PK15,PEF and IPI-2I cells,respectively)were found between the GFP knock-in and wild type cells by Western ***-seq results revealed that the transcriptome of GFP knock-in PEF cells had a significant positive correlation(P<2.2e–16)with that of the wild type cells,indicating that the GFP knock-in did not alter the global expression of endogenous ***,the CCK8 assay showed that the GFP knock-in events had no adverse effects(P_(24)h=0.31,P_(48)h=0.96,P_(72)h=0.24,P_(96)h=0.17,and P_(120)h=0.38)on cell proliferation of PK15 *** results indicate that the COL1A1 locus can be used as a safe harbor for foreign genes knock-in into the pig genome and can be broadly applied to farm animal breeding and biomedical model establishment.

关键词： COL1A1 gene safe harbor knock-in CRISPR/Cas9 pig

高地应力软岩隧道仰拱结构受力特性与参数优化

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公路交通科技 2024年第1期41卷 126-137,159页

作者：陈建勋牛嘉伟刘伟伟罗彦斌陈丽俊长安大学公路学院陕西西安710064

为探索高地应力软岩隧道施工期间仰拱结构受力特性与合理支护参数,以陕西省宝汉高速公路连城山隧道工程为依托,针对仰拱施工病害及其主要影响因子,采用现场试验与数值模拟相结合的方法,探讨了不同影响因素下的仰拱结构变形与力学响应,... 详细信息

为探索高地应力软岩隧道施工期间仰拱结构受力特性与合理支护参数,以陕西省宝汉高速公路连城山隧道工程为依托,针对仰拱施工病害及其主要影响因子,采用现场试验与数值模拟相结合的方法,探讨了不同影响因素下的仰拱结构变形与力学响应,分析了仰拱结构变形与受力对不同影响因素的敏感性,基于仰拱结构病害特征及数值模拟结果,对仰拱半径(深度)、仰拱厚度等支护参数进行了优化,并进行了工程验证。结果表明:连城山隧道施工期间仰拱二次衬砌最大接触压力达1.034 MPa,混凝土最大应力超过其抗压强度(30 MPa),出现了仰拱回填隆起、开裂等病害,仰拱设计支护参数难以保障结构稳定;地应力水平和基底围岩劣化对仰拱结构变形与受力状态均有显著影响,其中基底软化深度的影响最为突出,地应力水平次之;减小仰拱半径、增大仰拱厚度、增大仰拱钢筋直径、减小仰拱钢筋间距均能显著抑制仰拱变形,而提高混凝土强度等级对控制仰拱变形效果不明显,且经济性较差;优化后的仰拱受力得以明显改善,有效控制了仰拱结构病害,避免了频繁拆换仰拱,保障了隧道结构安全稳定。研究成果可为类似软岩隧道仰拱变形控制及病害防治提供重要借鉴。

关键词：隧道工程受力特性现场试验参数优化仰拱软岩

In situ formation of fluorescent silicon-containing polymer dots for alkaline phosphatase activity detection and immunoassay

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Science China Chemistry 2020年第4期63卷 554-560页

作者： Guoyong liu Jiahui Zhao Mengxia Yan Shuyun Zhu Wenchao Dou jian Sun Xiurong Yang State Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied ChemistryChinese Academy of SciencesChangchun 130022China University of Science and Technology of China Hefei 230026China University of Chinese Academy of Sciences Beijing 100049China College of Chemistry and Chemical Engineering Qufu Normal UniversityQnfu 273165China Food Safety Key Laboratory of Zhejiang Province College of Food Science and BiotechnologyZhejiang Gongshang UniversityHangzhou 310018China

The discovery and application of analyte-triggered fluorophore generation or fluorogenic reaction are significant and beneficial to the development of novel fluorescence(FL) analysis method. In this study, for the first time, we have reported a fluorogenic reaction to prepare fluorescent silicon-containing polymer dots(Si-PDs) by simply mixing N-[3-(trimethoxysilyl)propyl]ethylenediamine(DAMO) and hydroquinone(HQ) in aqueous solution at ambient temperature. Inspired by the alkaline phosphatase(ALP)-catalyzed hydrolysis of the substrate sodium 4-hydroxyphenyl phosphate(4-HPP) into HQ and the resultant HQcontrolled intense green Si-PDs generation, we have established a straightforward ALP activity assay by innovatively employing commercially available 4-HPP as the substrate. More significantly, the specific preparation method, clear formation mechanism and excellent performance enable the Si-PDs as well as its generation process to develop facile and attractive FL *** the help of the universal ALP-based enzyme-linked immunosorbent assay(ELISA) platform and corresponding antibody, a convenient and conceptual ALP-based fluorescent ELISA has been constructed and applied in sensing cardiac troponin Ⅰ(cTnI),a well-known biomarker of acute myocardial infarction. Our research via in situ formation of fluorescent nanomaterials has great potential application in ALP activity assay, inhibitor screening, and disease diagnosis.

关键词： fluorescent polymer dots ELISA alkaline phosphatase cardiac troponinⅠ

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非均匀沉降区埋地管道力学响应研究

兰州理工大学学报 2024年第3期50卷 64-68页

作者：卢国宏谢锐郭建明罗飞刘小波张杰山东省鲁南地质工程勘察院(山东省地质矿产勘查开发局第二地质大队) 山东济宁272100 四川文理学院智能制造学院四川达州635000 中国石油集团东方地球物理勘探有限责任公司河北涿州072750 聪明猪检测技术(成都)有限公司四川成都610041 国家管网集团西南管道昆明输油气分公司云南昆明650000 西南石油大学石油天然气装备技术四川省科技资源共享服务平台四川成都610500 自然资源部采煤沉陷区综合治理工程技术创新中心山东济宁272100

地层非均匀沉降是埋地管道面临的常见地质灾害之一,影响管道安全运行.为此建立地层沉降区管土耦合模型,研究了地层沉降量、管道壁厚、内压、管材对管道屈曲模式和力学性能的影响.结果表明:地层沉降作用下管道轴向拉伸应变和压缩应变集... 详细信息

地层非均匀沉降是埋地管道面临的常见地质灾害之一,影响管道安全运行.为此建立地层沉降区管土耦合模型,研究了地层沉降量、管道壁厚、内压、管材对管道屈曲模式和力学性能的影响.结果表明:地层沉降作用下管道轴向拉伸应变和压缩应变集中在沉降过渡区;沉降量较小时管道表面应变呈波纹状分布,沉降较大时管道产生局部屈曲;相同工况下,薄壁管道易发生屈曲失效,厚壁管道出现波纹状应变分布;随着内压增大,管道屈曲程度逐渐减弱;高钢级管道的屈曲程度越小.

关键词：地层沉降 X80管道轴向应变屈曲失效

Resistance of the flow over rough surfaces

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Journal of Hydrodynamics 2021年第3期33卷 593-601页

作者： Yu Han Shi-yu Wang jian Chen Shuqing Yang liu-chao Qiu Nadeesha Dharmasir College of Water Resources and Civil Engineering China Agricultural UniversityBeijing100083China College of Engineering China Agricultural UniversityBeijing100083China School of Civil Mining and Environmental EngineeringUniversity of WollongongWollongongAustralia

The mechanism of the flow resistance in open channels and pipelines is of vital importance for various critical issues related to the water *** Nikurade’s method of calculating the friction factor is not applicable in some *** is necessary to consider the influence of the vortex volume surrounding the vegetation and to study the hydrodynamic characteristics of the vegetated *** paper analyzes the variation of the vortices created by the surface roughness of different types and different sizes and proposes new definitions of the hydraulic radius and the equivalent roughness *** consideration of the skin friction and the form drag,on the basis of the force balance equation,a novel calculation method is used for the friction factor,and this method is verified by experimental *** hydrodynamic mechanism of the flow resistance revealed in this study may serve as a theoretical basis for hydraulic engineers to calculate the friction factor.

关键词： Hydraulic radius skin friction form drag friction factor separation zone dead fluid volume

New progress and future prospects of CBM exploration and development in China

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International Journal of Mining Science and Technology 2012年第3期22卷 363-369页

作者： Vun jian Xu Fengyin liu Lin Zhong Ningning Wu Xiaobin College of Geosciences China University of Petroleum Beijing 102249 China petroChina Coalbed Methane Company Limited Beijing 100028 China

Efforts to speed up China's coal bed methane (CBM) exploration developments related to production safety, optimization of energy structures, prevention of energy waste and reduction of greenhouse gas emissions are all of great significance. In order to strengthen CBM exploration and development in China and to encourage increased growth in the CBM industry, we firstly give a general overview of the recent technological innovations and other developments in CBM exploration in the U.S., Canada and other countries. Using this background information as the starting point, we further present observations and analyses of CBM exploration and development, preferential policies, technical support and implications of R&D for CBM development in China. The results show that the problems related to CBM exploration technology development and lack of a complete set of management policies are still the major issues slowing down the growth of domestic CBM industry. Development of resource exploration and technology, R&D and establishment of favorable government policy to support the industry and the creation of a relevant information platform, etc. are finally recommended.

关键词： ChinaCBMExploration and developmentProspectProposal

Hierarchically porous Cu current collector with lithiophilic CuxO interphase towards high-performance lithium metal batteries

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Journal of Energy Chemistry 2021年第7期30卷 292-299页

作者： Yaya Wang Zexu Zhao Wei Zeng Xingbo liu Lei Wang jian Zhu Bingan Lu State Key Laboratory for Chemo/Biosensing and Chemometrics and College of Chemistry and Chemical EngineeringHunan Key Laboratory of Two-Dimensional MaterialsHunan UniversityChangsha 410082HunanChina State Key Laboratory of Advanced Design and Manufacturing for Vehicle Body Hunan Provincial Key Laboratory of Intelligent Laser ManufacturingHunan UniversityChangsha 410082HunanChina School of Materials and Engineering Hunan UniversityChangsha 410082HunanChina School of Physics and Electronics Hunan UniversityChangsha 410082HunanChina

Lithium metal is one of the most promising anode materials for next-generation electrochemical energy storage due to low electrochemical potential and high specific ***,large volume change and uncontrollable formation of lithium dendrite during cycling severely hinder the practical application of rechargeable Li metal ***,we report a hierarchically porous Cu covered with lithiophilic CuxO(HPCu-CuxO) via femtosecond laser strategy in about 2 min as current collector for highperformance Li metal *** precisely tunable pore volume and depth as well as lithiophilic CuxO interphase,the HPCu-CuxO not only guides homogeneous Li nucleation,resulting in a smooth and dendrite-free lithium surface,but also provides space to alleviate the volume expansion of Li metal anode,achieving excellent structure ***,highly stable Coulombic efficiency and ultralow overpotential of 15 mV even up to 1000 h were achieved at the current density of 1 mA cm^(-2).Moreover,the resultant Li@HPCu-CuxO//LiFePO_(4) full battery delivered outstanding cycle stability and rate *** results offer a pathway toward high-energy-density and safe rechargeable Li metal batteries.

关键词： Lithium metal batteries Femtosecond laser strategy Hierarchically porous structure Lithiophilic CuxO interphase

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俄乌冲突侦察监视与伪装对抗应用分析

激光与红外 2024年第4期54卷 620-625页

作者：刘洪蕊王结良吴剑锋胡志毅苏海涛 32182部队北京100042

在战场日臻透明和“发现即摧毁”的侦察打击背景下,伪装成为提高军事目标生存和掩护军事行动的有效手段。本研究以俄乌冲突为背景,对覆盖空-天-地-海范围的侦察监视和各类伪装对抗措施进行系统分析。最后,从自适应多谱段伪装技术开发、... 详细信息

在战场日臻透明和“发现即摧毁”的侦察打击背景下,伪装成为提高军事目标生存和掩护军事行动的有效手段。本研究以俄乌冲突为背景,对覆盖空-天-地-海范围的侦察监视和各类伪装对抗措施进行系统分析。最后,从自适应多谱段伪装技术开发、轻量化及大跨度伪装器材研制、多维度伪装策略应用及效果评估等方面提出伪装对抗发展方向。

关键词：俄乌冲突侦察监视伪装对抗