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Chromatin-remodeling complexes:Conserved and plant-specific subunits in Arabidopsis

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Journal of Integrative Plant Biology 2022年第2期64卷 499-515页

作者： ji-Yun Shang Xin-jian He National Institute of Biological Sciences Beijing102206 China Tsinghua Institute of Multidisciplinary Biomedical Research Tsinghua UniversityBeijing100084 China

Adenosine triphosphate-dependent chromatin remodeling complexes are important for the regulation of transcription,DNA replication,and genome stability in eukaryotes.Although genetic studies have illustrated various biological functions of core and accessory subunits of chromatin-remodeling complexes in plants,the identification and characterization of chromatin-remodeling complexes in plants is lagging behind that in yeast and animals.Recent studies determined whether and how the Arabidopsis SWI/SNF,ISWI,INO80,SWR1,and CHD chromatin remodelers function in multi-subunit complexes in Arabidopsis.Both conserved and plant-specific subunits of chromatin-remodeling complexes have been identified and characterized.These findings provide a basis for further studies of the molecular mechanisms by which the chromatinremodeling complexes function in plants.

关键词： ATPase chromatin remodeling complex INO80 ISWI SWI/SNF SWR1 transcription

Effect of three-volt moxibustion with helium-neon laser irradiation on quality of care in patients with lumbar radiculopathy spondylosis

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World Journal of Clinical Cases 2024年第15期12卷 2522-2528页

作者： Xiu-Kun ji jian Li Department of Traditional Chinese Medicine The Second Hospital of Heilongjiang ProvinceHarbin 150028Heilongjiang ProvinceChina Departments of Four Neurology The Second Hospital of Heilongjiang ProvinceHarbin 150028Heilongjiang ProvinceChina

BACKGROUND Lumbar radiculopathy spondylosis is a relatively common orthopedic disease with a high incidence rate.It most commonly occurs in the lumbar 4-5 and lumbar 5-sacral 1 vertebrae,which account for approximately 95%of cases.It mostly occurs in people aged 30-50 years old and greatly affects their quality of life.AIM To determine the effect of triple-voltage acupuncture combined with helium-neon laser irradiation on the quality of care and improvement of symptoms in patients with lumbar radiculopathy spondylolisthesis.METHODS In this study,we selected 120 patients with lumbar radiculopathy spondylosis who were treated at our hospital between June 2019 to June 2020.The patients were divided into control and observation groups according to the random number table method,with 60 patients in each group.Patients in the observation group were treated with three-volt moxibustion combined with helium-neon laser irradiation,and those in the control group were treated with lumbar traction.After 1 month of treatment,the lumbar pain scores,lumbar spine motor functions,clinical treatment effects,and nursing satisfaction of the two groups were compared.RESULTS The results showed that acupuncture combined with laser irradiation significantly improved the patients'clinical symptoms,i.e.,reduced their low back pain,significantly lower numerical rating scale pain scores in the observation group than in the control group,and better lumbar spine motility than in the control group,compared to lumbar traction.In addition,they were cared for.The treatment effectiveness rate of the observation group was 95.5%,which was significantly higher than that of the control group(81.67%).Satisfaction with care was higher than 90 points in both groups,but the difference was not statistically significant.CONCLUSION Our study provides a clinical rationale for the future treatment of patients with lumbar spine disease.However,further extensive research is needed for validation.

关键词： Moxibustion Laser irradiation Clinical rationale Lumbar spine Three-volt moxibustion

A New Sufficient Degree Condition for a Graphic Sequence to Be Forcibly k-Edge-Connected

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Acta Mathematicae Applicatae Sinica 2022年第1期38卷 223-228页

作者： jian-hua YIN ji-yun GUO School of Science Hainan UniversityHaikou 570228China

A graphic sequence π =(d1, d2,..., dn) is said to be forcibly k-edge-connected if every realization of π is k-edge-connected. In this paper, we obtain a new sufficient degree condition for π to be forcibly k-edgeconnected. We also show that this new sufficient degree condition implies a strongest monotone degree condition for π to be forcibly 2-edge-connected and a conjecture about a strongest monotone degree condition for π to be forcibly 3-edge-connected due to Bauer et al.(Networks, 54(2)(2009) 95-98), and also implies a strongest monotone degree condition for π to be forcibly 4-edge-connected.

关键词： graphic sequence forcibly k-edge-connected graphic sequence a strongest monotone degree condition

Ratiometrically Designed Nanocarrier to Impact Major Cancer Pathways for Effective Pancreatic Cancer Treatment

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Chemical Research in Chinese Universities 2020年第6期36卷 1143-1144页

作者： ji jian Key Laboratory of Macromolecule Synthesis and Functionalization of Ministry of Education Department of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027P.R.China

To achieve secured in vivo drug synergy in stroma-rich pancreatic cancer(PDAC)is not an easy task.ji and Liu et al.demonstrate the rational development of a ratiometrically designed nanocarrier to co-package a CDK4/6 cel cycle inhibitor palbociclib(PAL)plus an autophagy inhibitor hydroxychloroquine(HCQ)for efficient PDAC treatment The lead formulation was obtained using a computer software aided in vitro screening,followed by a remote drug co-import into a lipid-coated mesoporous silica nanoparticle.The ratiometric nanocarrier led to the synchronized phar-macokinetic(PK)profile and effective intratumoral buildup of the drug pair post intravenous injection,which otherwise exhibited distinctly different biodistribution characteristics.The treatment using PAL/HCQ co-delivery nanoparticle:yielded the most effective shrinkage of PDAC in subcutaneous and orthotopic PANCI mouse models.The mechanistic investigation also demonstrated the activation of anti-apoptosis pathway after repetitive nanoparticle injection in mice which promoted the authors to introduce a small molecule anti-apoptosis inhibitor to further improve the performance of their already potent nanoparticle in the PDAC mouse model.This work has been published online in Nature Communi cations on August 25,2020.

关键词： injection otherwise treatment

Inverse reliability analysis and design for tunnel face stability considering soil spatial variability

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Journal of Rock Mechanics and Geotechnical Engineering 2024年第5期16卷 1552-1564页

作者： Zheming Zhang Jian Ji Xiangfeng Guo Siang Huat Goh Key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering Hohai UniversityNanjing210098China Department of Civil and Environmental Engineering National University of SingaporeSingapore

The traditional deterministic analysis for tunnel face stability neglects the uncertainties of geotechnical parameters,while the simplified reliability analysis which models the potential uncertainties by means of random variables usually fails to account for soil spatial variability.To overcome these limitations,this study proposes an efficient framework for conducting reliability analysis and reliability-based design(RBD)of tunnel face stability in spatially variable soil strata.The three-dimensional(3D)rotational failure mechanism of the tunnel face is extended to account for the soil spatial variability,and a probabilistic framework is established by coupling the extended mechanism with the improved Hasofer-Lind-Rackwits-Fiessler recursive algorithm(iHLRF)as well as its inverse analysis formulation.The proposed framework allows for rapid and precise reliability analysis and RBD of tunnel face stability.To demonstrate the feasibility and efficacy of the proposed framework,an illustrative case of tunnelling in frictional soils is presented,where the soil's cohesion and friction angle are modelled as two anisotropic cross-correlated lognormal random fields.The results show that the proposed method can accurately estimate the failure probability(or reliability index)regarding the tunnel face stability and can efficiently determine the required supporting pressure for a target reliability index with soil spatial variability being taken into account.Furthermore,this study reveals the impact of various factors on the support pressure,including coefficient of variation,cross-correlation between cohesion and friction angle,as well as autocorrelation distance of spatially variable soil strata.The results also demonstrate the feasibility of using the forward and/or inverse first-order reliability method(FORM)in high-dimensional stochastic problems.It is hoped that this study may provide a practical and reliable framework for determining the stability of tunnels in complex soil strata.

关键词： Limit analysis Tunnel face stability Spatial variability HLRF algorithm Inverse reliability method

DHA induces adipocyte lipolysis through endoplasmic reticulum stress and the cAMP/PKA signaling pathway in grass carp(Ctenopharyngodon idella)

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Animal Nutrition 2023年第2期13卷 185-196页

作者： Xiaocheng Huang Chenchen Bian Hong ji Shanghong ji jian Sun College of Animal Science and Technology Northwest Agriculture and Forestry UniversityYangling 712100China

Docosahexaenoic acid(DHA)is a biologically active fatty acid that reduces the accumulation of lipids.However,the molecular mechanism underlying this process,particularly in fish,is not well understood.Recent studies show that endoplasmic reticulum(ER)stress triggers the activation of the unfolded protein response,which has been revealed to play an essential role in lipid metabolism.In this study,we explored the effect of DHA on ER stress and investigated the potential molecular mechanisms underlying DHA-induced adipocyte lipolysis in grass carp(Ctenopharyngodon idella)both in vivo and in vitro.We found that DHA remarkably reduced the triglyceride content,increased the secretion of glycerol,pro-moted lipolysis in adipocytes and evoked ER stress,whereas inhibiting ER stress using 4-phenyl butyric acid(4-PBA)inhibited the effects of DHA(P<0.05).These results implied that ER stress potentially participates in DHA-induced adipocyte lipolysis.Additionally,STF-083010,a specific inositol-requiring enzyme 1a(IRE1a)-inhibitor,attenuated the effects of DHA on lipolysis,demonstrating that IRE1a and X-box binding protein 1 potentially participate in DHA-induced lipolysis.DHA also activated the cyclic adenosine monophosphate(cAMP)-dependent protein kinase A(PKA)pathway by increasing the level of cAMP and activating the PKA enzyme(P<0.05).Nevertheless,H89,a PKA inhibitor,weakened DHA-induced lipolysis by inhibiting the cAMP/PKA signaling pathway.Furthermore,inhibiting ER stress us-ing 4-PBA also inhibited lipolysis and alleviated DHA-induced activation of the cAMP/PKA signaling pathway,suggesting that ER stress may participate in DHA-induced lipolysis through the activation of the cAMP/PKA signaling pathway.Our data illustrate that DHA supplementation can be a promising nutritional strategy for ameliorating lipid accumulation in grass carp.The present study elucidated the molecular mechanism for DHA-induced lipolysis in grass carp adipocytes and emphasized the impor-tance of ER stress and the cAMP/PKA pathway in DHA-induced lip

关键词： Adipocyte cAMP/PKA pathway Docosahexaenoic acid Endoplasmic reticulum stress Grass carp Lipolysis

UV-triggered Polymerization of Polyelectrolyte Composite Coating with Pore Formation and Lubricant Infusion

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Chinese Journal of Polymer Science 2023年第3期41卷 365-372,I0004,I0006页

作者： jia-Qi Hu Wei-Pin Huang jing Wang Ke-Feng Ren Jian Ji MOE Key Laboratory of Macromolecular Synthesis and Functionalization International Research Center for X PolymersDepartment of Polymer Science and EngineeringZhejiang UniversityHangzhou 310027China

Porous coatings with the features of muti-interfaces and high specific surface area have emerged as an excellent material platform for the manipulation of porous structures.Layer-by-layer(Lb L)assembly technique has been widely used in preparing porous polyelectrolyte coatings.However,the efficient construction of stable film from the Lb L technique is still a question.Herein,we reported a new solution to construct a stabilized polyelectrolyte coating with porous structures.Inspired by the mechanical reinforcement of double-network hydrogel,we constructed the poly(ethylenimine)(PEI)/poly(acrylic acid)(PAA)coating by in situ photopolymerization of acrylic acid in the PEI network instead of assembling PEI and PAA.Compared with the Lb L films,the in situ polymerized coating kept higher stability after 30 iterations of friction.Porous structures could also be constructed after acid treatment,which was utilized to load lubricant to enhance the lubricating property of the coating.This work provides a new method for the construction of dynamic and stable polyelectrolyte coatings,expediting more development of practical applications.

关键词： Porous coating Polyelectrolyte coating Dip-coating UV-triggered polymerization Stability

A deep-learning-based workflow to deal with the defocusing problem in high-throughput experiments

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Bioactive Materials 2022年第5期7卷 218-229页

作者： Yunfan Xue Honglin Qian Xu Li jing Wang Kefeng Ren Jian Ji MOE Key Laboratory of Macromolecule Synthesis and Functionalization Department of Polymer Science and EngineeringZhejiang UniversityHangzhou310027PR China

The increasing throughput of experiments in biomaterials research makes automatic techniques more and more necessary.Among all the characterization methods,microscopy makes fundamental contributions to biomaterials science where precisely focused images are the basis of related research.Although automatic focusing has been widely applied in all kinds of microscopes,defocused images can still be acquired now and then due to factors including background noises of materials and mechanical errors.Herein,we present a deep-learning-based method for the automatic sorting and reconstruction of defocused cell images.First,the defocusing problem is illustrated on a high-throughput cell microarray.Then,a comprehensive dataset of phase-contrast images captured from varied conditions containing multiple cell types,magnifications,and substrate materials is prepared to establish and test our method.We obtain high accuracy of over 0.993 on the dataset using a simple network architecture that requires less than half of the training time compared with the classical ResNetV2 architecture.Moreover,the subcellular-level reconstruction of heavily defocused cell images is achieved with another architecture.The applicability of the established workflow in practice is finally demonstrated on the high-throughput cell microarray.The intelligent workflow does not require a priori knowledge of focusing algorithms,possessing widespread application value in cell experiments concerning high-throughput or time-lapse imaging.

关键词： High-throughput Deep learning Cell imaging Refocusing Microscopy

Access to 3-alkylselenindoles by multicomponent reaction of indoles,selenium powder and unactivated alkyl halides under transition-metal-free conditions

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Chinese Chemical Letters 2022年第9期33卷 4303-4305页

作者： Huan Liu Zhong-jian Cai Shun-Jun ji Key Laboratory of Organic Synthesis of jiangsu Province College of ChemistryChemical Engineering and Materials Science&Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow UniversitySuzhou 215123China

Herein,we reported a convenient and efficient multicomponent reaction of indoles,selenium powder and unactivated alkyl halides.This protocol provides a practical,and facile approach for the synthesis of 3-alkylselenindole derivatives.The advantages of this strategy include mild and transition-metal-free conditions,broad functional group tolerance,the use of simple and easily accessible seleniium powder and alkyl halides as coupling partners.More importantly,the reaction proceeded smoothly with a large scale(>10 g,>90%yield),which further highlighted the potential application of this selenation strategy.

关键词： Selenium 3-Alkylselenindole Indole Selenization