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Monoclonal Antibody-Based Serological Detection of Rice Stripe Mosaic Virus Infection in Rice Plants or Leafhoppers

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Virologica Sinica 2020年第2期35卷 227-234页

作者： Liqian Guo Jiayu wu Rui Chen jian hong Xueping Zhou jianxiang wu State Key Laboratory of Rice Biology Institute of Biotechnology.Zhejiang UniversityHangzhou 310058China Department of Applied Biological Science College of Agriculture and BiotechnologyZhejiang UniversityHangzhou 310058China State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China

Rice stripe mosaic virus(RSMV) is a rhabdovirus recently found in southern part of China and can cause severe reduction in rice production. To establish serological methods for RSMV epidemiological studies and to establish a control strategy for this virus, we first purified RSMV virions from infected rice plants and then used them as an immunogen to produce four RSMV-specific monoclonal antibodies(MAbs)(i.e.,1D4, 4A8, 8E4 and 11F11). With these MAbs, we have developed a highly specific and sensitive antigen-coated plate enzyme-linked immunosorbent assay(ACP-ELISA), a Dot-ELISA and a Tissue print-ELISA for rapid detections of RSMV infection in rice plants or in leafhoppers. Our results showed that RSMV can be readily detected in RSMV-infected rice plant tissue crude extracts diluted at 1:20,971,520(w/v, g/m L)through ACP-ELISA or diluted at 1:327,680(w/v, g/m L) through Dot-ELISA. Both ACP-ELISA and Dot-ELISA can also be used to detect RSMV infection in individual RSMV viruliferous leafhopper(Recilia dorsalis) homogenate diluted at 1:307,200 and 1:163,840(individual leafhopper/l L), respectively. Detection of RSMV infection in field-collected rice samples or in RSMV viruliferous leafhoppers indicated that the three serological methods can produce same results with that produced by RT-PCR(19 of the 33 rice samples and 5 of the 16 leafhoppers were RSMV-positive). We consider that the four MAbs produced in this study are very specific and sensitive, and the three new serological methods are very useful for detections of RSMV infection in rice plants or in leafhoppers and the establishment of the disease control strategies.

关键词： Rice stripe mosaic virus(RSMV) Serological test(ACP-ELISA) Dot-ELISA Tissue print-ELISA

Abnormal Nucleophilic Reaction of Ethyl Crotonate: An Easy Way to Form Novel Imidazo[1,5-a] pyridine derivatives

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Chinese Chemical Letters 2000年第11期11卷 949-950页

作者： Li, hong Mei Wang, Cong Wang, jian wu Sch. of Chem. and Environ. Science Shandong University Jinan Shandong 250100 China

An abnormal intramolecular nucleophilic reaction of α-carbon of α , β -unsaturated ester was discovered and the reaction makes it very easy to form imidazo[1,5-a]pyridine derivatives. The mechanism of the reaction ... 详细信息

关键词： pyridine derivatives Abnormal Nucleophilic Reaction of Ethyl Crotonate An Easy Way to Form Novel Imidazo[1,5-a Easy Form

Synthesis,characterization and herbicidal activity of new thiazoline derivatives

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Chinese Chemical Letters 2013年第3期24卷 233-235页

作者： jian-Chao Liu Ying Liang hong-wu He College of Chemistry Central China Normal University Hubei Biopesticide Engineering Research Center Hubei Academy of Agricultural Science

A series of new thiazoline derivatives 2-alkyl（aryl） imino-4-amino-5-ethoxycarbonyl-3-phenyl-3H- thiazoline （3） and bis（2-imino-4-amino-5-ethoxycarbonyl-3-phenyl-3H-thiazoline alkylene （4） have been synthesized by reactions of intermediate 4-amino-5-ethoxycarbonyl-2-methylthio-3-phenyl-3H- thiazolinium sulphate （2） with alkylamines or arylamine in 64%-89% yields. The structures of 3 and 4 have been confirmed by 1H NMR, El-MS, IR spectroscopy and elemental analyses. Some compounds exhibited high or moderate herbicidal activities against the roots of rapeseed and barnyard grass at 100 mg/L.

关键词： ThiazolineAlkylamineArylamineHerbicidal activity

Variation in carbon,nitrogen and phosphorus partitioning between above-and belowground biomass along a precipitation gradient at Tibetan Plateau

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Journal of Mountain Science 2016年第4期13卷 661-671页

作者： hong jiang-tao WANG Xiao-dan wu jian-bo Institute of Mountain Hazards and Environment Academy of Sciences of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China

Precipitation is a potential factor that significantly affects plant nutrient pools by influencing biomass sizes and nutrient concentrations. However, few studies have explicitly dissected carbon(C), nitrogen(N) and phosphorus(P) pools between above- and belowground biomass at the community level along a precipitation gradient. We conducted a transect(approx. 1300 km long) study of Stipa purpurea community in alpine steppe on the Tibet Plateau of China to test the variation of N pool of aboveground biomass/N pool of belowground biomass(AB/BB N) and P pool of aboveground biomass/P pool of belowground biomass(AB/BB P) along a precipitation gradient. The proportion of aboveground biomass decreased significantly from mesic to drier sites. Along the belt transect, the plant N concentration was relatively stable; thus, AB/BB N increased with moisture due to the major influences by above- and belowground biomass allocation. However, P concentration of aboveground biomass decreased significantly with increasing precipitation and AB/BB P did not vary with aridity because of the offset effect of the P concentration and biomass allocation. Precipitation gradients do decouple the N and P pool of a S. purpurea community along a precipitation gradient in alpine steppe. The decreasing of N:P in aboveground biomass in drier regions may indicate much stronger N limitation in more arid area.

关键词： Biomass allocation Nutrient concentration Qinghai-Tibetan Plateau Alpine steppe Stipa purpurea

基因重组大肠杆菌表达截短型pZP3β的发酵条件优化

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生殖与避孕 2006年第4期26卷 195-199,213页

作者：谢秋玲陈小佳朱伟杰赵振云吴建虹徐万祥刘侃李菁暨南大学生物工程研究所广州510632 广东省生物工程药物重点实验室广州510632 暨南大学生殖免疫研究中心广州510632 暨南大学生物系广州51063 上海市计划生育科学研究所上海200032 暨南大学医学院广州510632

目的:对已构建好的表达pZP3β蛋白(pZP130)工程菌的培养条件进行优化,通过在5L罐中发酵和Ni柱纯化,以期获取较大量目的产物。方法:在摇瓶中改变不同的培养及诱导条件,比较工程菌的生长和目的蛋白的表达,优化发酵条件;并在5L发酵罐中进... 详细信息

目的:对已构建好的表达pZP3β蛋白(pZP130)工程菌的培养条件进行优化,通过在5L罐中发酵和Ni柱纯化,以期获取较大量目的产物。方法:在摇瓶中改变不同的培养及诱导条件,比较工程菌的生长和目的蛋白的表达,优化发酵条件;并在5L发酵罐中进行发酵,获得菌体,破碎后进行Ni柱纯化。结果:优化的发酵条件是:10%的接种量,加入2%甘油的培养基培养菌体3h后加入0.5mmol/LIPTG,诱导4h后收样。在5L发酵罐中发酵,获得菌体量36g/L,经Ni柱纯化,获得蛋白粗制品33mg/L发酵液。结论:截短型pZP3β蛋白可以在***中获得较高表达,并且可以通过发酵和Ni柱纯化得到较大量的蛋白,这有助于pZP的深入研究和应用。

关键词：截短型猪卵透明带-3β 人肠杆菌表达发酵

Nest site selection of white-naped crane(Grus vipio) at Zhalong National Nature Reserve, Heilongjiang, China

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Journal of Forestry Research 2014年第4期25卷 947-952页

作者： Qing-ming wu hong-fei ZOU jian-zhang MA College of Wildlife Resource Northeast Forestry University

White-naped crane （Grus vipio） is a globally threatened spe- cies. It is very important to analyze its nest site selection in circum- stances where there are multiple disturbances, and also helpful to accu- mulate valuable information about this threatened species and supply scientific suggestions for conservation and management. We studied nest site selection and the effects of environmental variables on nesting habits of white-naped crane at Zhalong National Nature Reserve, Qiqihar City, Heilongiiang, China, during March-May of 2002-2008. White-naped crane responded and adapted to changes in the quality of the spatial environments of landscape and microhabitat under multiple environ- mental disturbances. Nest site selection included two scales and two choices, namely the choice of nest site habitat type within the macro-habitat scale and nest site micro-habitat selection within the mi- cro-habitat scale. Nest sites were recorded only in reed marshes. The choice of nest site micro-habitat included three basic elements and six factors, namely incubation element （nest parameters factor, incubation temperature factor and incubation humidity factor）, safety element （pro- tection factor and concealment factor）, and food element （water factor）. Water, remnant reed clusters, and fire were major resource management challenges during the breeding period for the white-naped crane in this Reserve.

关键词： factor analysis environmental variable Grus vipio nestsite selection pattern Zhalong Nature Reserve

Landscape design method for a green community based on green building design theory

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2010年第9期11卷 691-700页

作者： hong-feng GUO jian GE Miao YUE Xiao ZHOU wu JIN Architectural Design and Research Institute Zhejiang University Hangzhou 310027 China Department of Architecture Zhejiang University Hangzhou 310058 China

Landscape design for a green community should be favorable for the ambient ecological environment and for enhancement of both the local climate of a residential area and the environmental quality of life. This paper presents optimization methods for plant landscape design that take account of results of comparisons of noise reduction effects, heat island temperature decreases and effects on ventilation. These methods are based on different plant configurations and various luminous environments, such as exterior sunshine and shaded areas after the simulation of exterior luminous, acoustic and thermal environments of a residential area using the analytic software ECOTECT, CADNA/A, and PHEONICS. Three different types of residential buildings are simulated including a faculty apartment in the Xixi Campus of Zhejiang University, the Huaqing Villa and the Gangwan Jiayuan faculty apartment of Zhejiang University, China, based on green building design theory. In addition, the methods and process flow of landscape design of green residential areas are also described for the improvement of the exterior physical living environment.

关键词： Landscape design Green community Green building Exterior physical environment

Comparisons and applications of numerical simulation methods for predicting aerodynamic heating around complex configurations

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Acta Mechanica Sinica 2011年第3期27卷 339-345页

作者： Jin-Ling Luo hong-Lin Kang jian Li wu-Ye Dai Northwestern Polytechnical University 710072 Xi'an China Beijing Electro-Mechanical Engineering Institute100074 Beijing China

Numerical simulation methods of aerodynamic heating were compared by considering the inuence of numerical schemes and turbulence models,and attempting to investigate the applicability of numerical simulation methods on predicting heat flux in engineering applications. For some typical cases provided with detailed experimental data,four spatial schemes and four turbulence models were adopted to calculate surface heat flux. By analyzing and comparing,some inuencing regularities of numerical schemes and turbulence models on calculating heat flux had been acquired. It is clear that AUSM＋-up scheme with rapid compressibilitymodified high Reynolds number k鈥撓？model should be appropriate for calculating heat flux. The numerical methods selected as preference above were applied to calculate the heat flux of a 3-D complex geometry in high speed turbulent flows. The results indicated that numerical simulation can capture the complex flow phenomena and reveal the mechanism of aerodynamic heating. Especially,the numerical result of the heat flux at the stagnation point of the wedge was well in agreement with the prediction of Kemp鈥揜iddel formula,and the surface heat flux distribution was consistent with experiment results,which implied that numerical simulation can be introduced to predict heat flux in engineering applications.

关键词： Aerodynamic heating . Complex configurations . Numerical simulation

Spectrofluorimetric Determination of Trace Amounts of Gallium with Salicylaldehyde Benzoylhydrazone

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Chemical Research in Chinese Universities 1998年第3期14卷 37-41页

作者： TANG Bo wu Chang ju WANG hong jian YEN jian chong Department of Chemistry Shandong Normal UniversityJinan250014

The fluorescent reagent salicylaldehyde benzoylhydrazone(SABH) was synthesized and its ionization constants were estimated spectrophotometrically. The fluorescent reaction of this reagent with gallium was studied. Based on this chelation reaction, a spectrofluorimetric method was developed for the determination of gallium in a water medium at pH 3.2. Under these conditions, the Ga SABH complex has excitation and emission maxima at 372 and 455 nm, respectively. The linear range of the method was 0～150 μg/L and the detection limit was 0.36 μg/L of gallium when a standard addition method was used in the assay. The molar ratio of gallium to the reagent was 1∶3. The interference of other ions was studied. The extraction with n butyl acetate from a 6 mol/L hydrochloric acid medium was used to separate Ga from the interfering elements in semiconductor silicon and geological samples, and themethod has been successfully applied to the determination of gallium in these samples.

关键词： Salicylaldehyde benzoylhydrazone Gallium determination Spectrofluorimetry