检索结果-南通市图书馆

Biomedical and Environmental Sciences 2024年第6期37卷 565-580页

作者： Meng Wu jialu Zhou Zhe zhang Yuanguang Meng School of Medicine Nankai UniversityTianjin 300071China Department of Obstetrics and Gynecology The First Medical Center of Chinese PLA General HospitalBeijing 100853China Department of Obstetrics and Gynecology Chinese PLA Medical SchoolBeijing 100853China Department of Obstetrics and Gynecology The Seventh Medical Center of Chinese PLA General HospitalBeijing 100700China

Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy.RNA Sequencing was performed to analyze neoantigen expression.Results Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in CSCCs.Missense mutations were the most frequent types of somatic mutation in the coding sequence regions.Mutational signature analysis detected signature 2,signature 6,and signature 7 in CSCC samples.PIK3CA,FBXW7,and BICRA were identified as potential driver genes,with BICRA as a newly reported gene.Genomic variation profiling identified 4,960 potential neoantigens,of which 114 were listed in two neoantigen-related databases.Conclusion The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.

关键词： Cervical squamous cell carcinoma Genome alteration Neoantigens Immunotherapy

Self-fueling ferroptosis-inducing microreactors based on p H-responsive Lipiodol Pickering emulsions enable transarterial ferro-embolization therapy

在线全文

学校读者我要写书评

暂无评论

National Science Review 2024年第1期11卷 74-85页

作者： Chunjie Wang Lei zhang Zhijuan Yang Dongxu Zhao Zheng Deng jialu Xu Yumin Wu Yu Hao Ziliang Dong Liangzhu Feng Zhuang Liu Institute of Functional Nano & Soft Materials (FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials & Devices Soochow University Center of Interventional Radiology & Vascular Surgery Department of Radiology Zhongda Hospital Medical School Southeast University Department of Interventional Radiology The First Afflliated Hospital of Soochow University

Lipiodol chemotherapeutic emulsions remain one of the main choices for the treatment of unresectable hepatocellular carcinoma(HCC) via transarterial chemoembolization(TACE). However, the limited stability of Lipiodol chemotherapeutic emulsions would lead to rapid drug diffusion, which would reduce the therapeutic benefit and cause systemic toxicity of administrated chemotherapeutics. Therefore, the development of enhanced Lipiodol-based formulations is of great significance to enable effective and safe TACE treatment. Herein, a stable water-in-oil Lipiodol Pickering emulsion(LPE) stabilized by pH-dissociable calcium carbonate nanoparticles and hemin is prepared and utilized for efficient encapsulation of lipoxygenase(LOX). The obtained LOX-loaded CaCO3&hemin-stabilized LPE(LHCa-LPE) showing greatly improved emulsion stability could work as a pH-responsive and self-fueling microreactor to convert polyunsaturated fatty acids(PUFAs), a main component of Lipiodol, to cytotoxic lipid radicals through the cascading catalytic reaction driven by LOX and hemin, thus inducing ferroptosis of cancer cells. As a result, such LHCa-LPE upon transcatheter embolization can effectively suppress the progression of orthotopic N1S1 HCC in rats. This study highlights a concise strategy to prepare pH-responsive and stable LPE-based self-fueling microreactors, which could serve as bifunctional embolic and ferroptosis-inducing agents to enable proof-of-concept transarterial ferro-embolization therapy of HCC.

关键词： CaCO3 nanoparticles Pickering emulsion ferroptosis lipid peroxidation transarterial ferro-embolization

Microscopic morphology evolution of the crystal structure of tetrahydrofuran hydrate under flowing condition

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2022年第5期35卷 103-110页

作者： jialu zhang Xiang Liu Shuai Liu Yuxing Li Qihui Hu Wuchang Wang Shandong Key Laboratory of Oil-Gas Storage and Transportation Safety College of Pipeline and Civil EngineeringChina University of Petroleum(East China)Qingdao 266580China State Key Loboratory of Natural Gas Hydrates Beijing 100028China

The evolution of the hydrate particle structure during growth and agglomeration under flowing condition affects the particle as well as flow characteristic,which plays an important role in the flow assurance as well as heat transfer in refrigeration systems.Therefore,this article conducts experiments to study and observe the growth and agglomeration process in the main forming stage of hydrate.It was found that the growth of tetrahydrofuran hydrate was anisotropic and in a layered growth pattern.Single crystals generally transformed from octahedral structure to octahedral skeleton structure with growth,however some single crystals also deformed into plate type particles.The thickness of the plate type particles increased gradually during growth,and the edge part increased earlier than the middle part.During agglomeration,the hydrate particles contacted and sintered together.Sand as the impurity didn’t serve as the nucleation center but affected the agglomeration of hydrate particles by collisions.In addition,the effect increased as the sand size decreased.Finally,a microstructure model for hydrate growth and agglomeration was proposed,which showed the hydrate structure evolution in these processes and could lay a foundation for studying the flow assurance of hydrate slurry.

关键词： Tetrahydrofuran hydrate Growth Agglomeration Morphology Microstructure Microstructure model

Destructing surfactant network in nanoemulsions by positively charged magnetic nanorods to enhance oil-water separation

在线全文

学校读者我要写书评

暂无评论

Journal of Environmental Sciences 2022年第8期34卷 112-121页

作者： Yongjiao Xiong Xiangfeng Huang Lexue Li Wanqi Liu jialu zhang Mengfan He Jia Liu Lijun Lu Kaiming Peng College of Environmental Science and Engineering State Key Laboratory of Pollution Control and Resource ReuseMinistry of Education Key Laboratory of Yangtze River Water EnvironmentTongji UniversityShanghai 200092China

The separation of ultrafine oil droplets from wasted nanoemulsions stabilized with high concentration of surfactants is precondition for oil reuse and the safe discharge of effluent.However, the double barriers of the interfacial film and network structures formed by surfactants in nanoemulsions significantly impede the oil-water separation. To destroy these surfactant protective layers, we proposed a newly-developed polyethyleneimine micelle template approach to achieve simultaneous surface charge manipulation and morphology transformation of magnetic nanospheres to magnetic nanorods. The results revealed that positively charged magnetic nanospheres exhibited limited separation performance of nanoemulsions, with a maximum chemical oxygen demand(COD) removal of 50%, whereas magnetic nanorods achieved more than 95% COD removal in less than 30 s. The magnetic nanorods were also applicable to wasted nanoemulsions from different sources and exhibited excellent resistance to wide pH changes. Owing to their unique one-dimensional structure, the interfacial dispersion of magnetic nanorods was significantly promoted, leading to the efficient capture of surfactants and widespread destruction of both the interfacial film and network structure, which facilitated droplet merging into the oil phase. The easy-toprepare and easy-to-tune strategy in this study paves a feasible avenue to simultaneously tailor surface charge and morphology of magnetic nanoparticles, and reveals the huge potential of morphology manipulation for producing high-performance nanomaterials to be applied in complex interfacial interaction process. We believe that the newly-developed magnetic-nanorods significantly contribute to hazardous oily waste remediation and advances technology evolution toward problematic oil-pollution control.

关键词： Nanoemulsions Surface charge Magnetic nanorods Surfactant Interfacial film Oil-water separation

Construction and verification of an infectious cDNA clone of coxsackievirus B5

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2022年第3期37卷 469-471页

作者： Lifang Song Bopei Cui Jinghuan Yang Xiaotian Hao Xujia Yan jialu zhang Dong Liu Ziyang Song Qian Wang Qunying Mao Zhenglun Liang National Institute for Food and Drug Control Beijing 102629China Wuhan Institute of Biological Products CO. LTDWuhan 430070China

Dear Editor,Enteroviruses belonging to the family Picornaviridae are nonenveloped RNA viruses that cause hand-foot-mouth disease(HFMD),which can lead to severe neurological complications.Enteroviruses genomes represen... 详细信息

关键词： neurological envelope translated

在线全文

学校读者我要写书评

暂无评论

中美贸易摩擦与企业研发投入

国际贸易问题 2024年第4期 121-140页

作者：解维敏郭佳璐张恒鑫东北财经大学会计学院

攻克关键核心技术是掌握科技发展主动权,更好地应对外部环境不确定性的重要举措。基于实物期权理论,本文通过整理2018年美国征税清单数据,构建双重差分模型检验中美贸易摩擦对中国企业研发投入的影响。研究发现,中美贸易摩擦会促进受制... 详细信息

攻克关键核心技术是掌握科技发展主动权,更好地应对外部环境不确定性的重要举措。基于实物期权理论,本文通过整理2018年美国征税清单数据,构建双重差分模型检验中美贸易摩擦对中国企业研发投入的影响。研究发现,中美贸易摩擦会促进受制裁企业增加研发投入,且该结论在一系列稳健性检验后依然成立。机制检验表明,规避市场竞争与成本压力效应是中美贸易摩擦影响企业研发投入的路径机制。异质性分析发现,中美贸易摩擦对企业研发投入的促进作用在一般贸易占比高、全球价值链嵌入程度深以及不存在海外投资的企业中更显著。进一步分析显示,中美贸易摩擦不仅会增加企业研发投入,还有利于提高创新产出和创新质量,促使企业实施自主创新策略。本文为中美贸易摩擦经济后果的相关研究提供了新证据,也为破解当前“卡脖子”难题与实现经济高质量发展提供了有益参考和政策建议。

关键词：中美贸易摩擦企业研发投入实物期权理论

Comparative study of gel-based separated arcdischarge, HiPCO, and CoMoCAT carbon nanotubes for macroelectronic applications

在线全文

学校读者我要写书评

暂无评论

Nano Research 2013年第12期6卷 906-920页

作者： jialu zhang Hui Gui Bilu Liu Jia Liu Chongwu Zhou Department of Electrical Engineering University of Southern California Los Angeles California 90089 USA Department of Material Science University of Southern Califomia Los Angeles California 90089 USA Department of Chemistry University of Southern California Los Angeles California 90089 USA

由于他们有房间温度免职 / 出版处理的优秀电的性质和相容性，为 macroelectronic 应用程序例如的高纯净的围单人赛的半导体的碳 nanotubes 抓住伟人潜力在薄电影的晶体管和显示器背面板电子学。然而，亲戚有利并且为 macroelectronics... 详细信息

由于他们有房间温度免职 / 出版处理的优秀电的性质和相容性，为 macroelectronic 应用程序例如的高纯净的围单人赛的半导体的碳 nanotubes 抓住伟人潜力在薄电影的晶体管和显示器背面板电子学。然而，亲戚有利并且为 macroelectronics 遗体的各种各样的 nanotubes 的劣势一个开的问题，尽管有大意义。这里在这糊，我们报导三种主流的碳 nanotubes 的比较、系统的研究(弧分泌物， HiPCO ， CoMoCAT )为薄电影的晶体管应用使用便宜基于胶化的专栏层析分开，并且高效晶体管--它为在活跃矩阵使用的晶体管满足要求器官的轻射出的二极管显示有完成。我们观察在取决于 nanotube 直径的晶体管活动性和开／关比率之间的一宗交易。当时有更大的直径的弧分泌物 nanotubes 导致高设备活动性， HiPCO 和 CoMoCAT 有更小的直径的 nanotubes 能提供高开／关比率(> 106 ) 为有可比较的尺寸的晶体管。而且，我们也把基于胶化的分开的 nanotubes 与 nanotubes 用密度坡度超离心机(DGU ) 分开了作比较方法，并且发现分开胶化的 nanotubes 能像分开 DGU 的 nanotubes 一样提供纯净和薄电影的晶体管性能。我们的途径能为未来碳用作批评基础基于 nanotube 的薄电影的 macroelectronics。

关键词：半导体碳纳米管 ic应用有机发光二极管显示器分离凝胶薄膜晶体管电弧放电电性能

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

碳基固体氧化物燃料电池研究进展

洁净煤技术 2024年第1期30卷 239-264页

作者：张伟魏嘉璐 JOSE Antonio Alonso 孙春文中国矿业大学(北京)化学与环境工程学院北京100083 Instituto de Ciencia de los Materiales de Madrid CSICCantoblanco-Madrid 28049Spain

随着人们对绿色能源和环境友好型社会需求的日益增长,先进能量转换装置或系统的研发亟待加速,而燃料电池系统被认为是最有希望的未来发电装置。在不同种类的燃料电池中,固体氧化物燃料电池(SOFCs)是一种清洁环保的发电装置,因其独特的... 详细信息

随着人们对绿色能源和环境友好型社会需求的日益增长,先进能量转换装置或系统的研发亟待加速,而燃料电池系统被认为是最有希望的未来发电装置。在不同种类的燃料电池中,固体氧化物燃料电池(SOFCs)是一种清洁环保的发电装置,因其独特的多燃料运行能力及能量转化效率高等优点而前景广阔。然而,传统使用镍-金属陶瓷阳极的SOFCs,在碳氢化合物燃料下运行时,由于燃料的不完全氧化会使阳极产生积碳,从而导致电池性能下降。讨论了碳基SOFC中碳沉积机理和解决阳极积碳的策略,重点介绍了目前广泛研究的具有混合离子-电子导体的双金属-金属陶瓷材料的种类以及研究进展,并总结了双金属-金属陶瓷材料的抗积碳机理,主要表现为降低阳极表面的碳沉积速率以及加速积碳的去除。还介绍了在还原性气氛中,钙钛矿材料中纳米粒子原位偏析的原理,并讨论了原位偏析所形成的纳米颗粒以及其与钙钛矿主体所形成的金属氧化物异质结构在抗积碳阳极中的应用。此外,还探究了多层阳极和单原子催化剂阳极在中温SOFCs中的应用。在燃料电池技术巨大进步的推动下,人们对可用于固定式发电的集成煤气化的燃料电池发电系统(IGFCs)产生了新的兴趣,该系统对于实现煤炭的清洁高效利用具有重要意义,该技术的关键依托于SOFCs的成熟度。最后,总结并指出了碳基SOFCs未来的一些研究方向。

关键词：固体氧化物燃料电池阳极材料碳氢燃料碳沉积整体煤气化燃料电池

Immunogenicity and protective efficacy of a recombinant protein subunit vaccine and an inactivated vaccine against SARS-CoV-2 variants in non-human primates

在线全文

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2022年第4期7卷 1170-1180页

作者： Qian He Qunying Mao Xiaozhong Peng Zhanlong He Shuaiyao Lu jialu zhang Fan Gao Lianlian Bian Chaoqiang An Wenhai Yu Fengmei Yang Yanan Zhou Yun Yang Yanyan Li Yadi Yuan Xujia Yan Jinghuan Yang Xing Wu Weijin Huang Changgui Li Junzhi Wang Zhenglun Liang Miao Xu Division of Hepatitis and Enterovirus Vaccines Institute of Biological ProductsNational Institutes for Food and Drug ControlNHC Key Laboratory of Research on Quality and Standardization of Biotech ProductsNMPA Key Laboratory for Quality Research and Evaluation of Biological ProductsBeijing 102629People’s Republic of China Institute of Medical Biology Chinese Academy of Medicine Sciences&Peking Union Medical CollegeKunming 650118People’s Republic of China

Emerging SARS-CoV-2 variants and the gradually decreasing neutralizing antibodies over time post vaccination have led to an increase in incidents of breakthrough infection across the world.To investigate the potential protective effect of the recombinant protein subunit COVID-19 vaccine targeting receptor-binding domain(RBD)(PS-RBD)and whole inactivated virus particle vaccine(IV)against the variant strains,in this study,rhesus macaques were immunized with PS-RBD or IV vaccine,followed by a Beta variant(B.1.351)challenge.Although neutralizing activity against the Beta variant was reduced compared with that against the prototype,the decreased viral load in both upper and lower respiratory tracts,milder pathological changes,and downregulated inflammatory cytokine levels in lung tissues after challenge demonstrated that PS-RBD and IV still provided effective protection against the Beta variant in the macaque model.Furthermore,PS-RBD-induced macaque sera possessed general binding and neutralizing activity to Alpha,Beta,Delta,and Omicron variants in our study,though the neutralizing antibody(NAb)titers declined by varying degrees,demonstrating potential protection of PS-RBD against current circulating variants of concern(VOCs).Interestingly,although the IV vaccine-induced extremely low neutralizing antibody titers against the Beta variant,it still showed reduction for viral load and significantly alleviated pathological change.Other correlates of vaccine-induced protection(CoP)like antibody-dependent cellular cytotoxicity(ADCC)and immune memory were both confirmed to be existing in IV vaccinated group and possibly be involved in the protective mechanism.

关键词： protective vaccine neutral

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

米老排优树组培技术体系优化研究

南京林业大学学报（自然科学版） 2024年第2期48卷 69-78页

作者：孙旭高陶家璐谢微石洁张宝津邓小梅华南农业大学林学与风景园林学院广东省森林植物种质创新与应用重点实验室广东广州510642

【目的】建立米老排(Mytilaria laosensis)优良单株组培再生体系,为今后其无性化推广提供技术支撑。【方法】以米老排优树基部半木质化萌芽为外植体,采用丛芽发生途径,通过初代腋芽诱导、继代增殖培养、生根培养等过程,研究筛选最适的... 详细信息

【目的】建立米老排(Mytilaria laosensis)优良单株组培再生体系,为今后其无性化推广提供技术支撑。【方法】以米老排优树基部半木质化萌芽为外植体,采用丛芽发生途径,通过初代腋芽诱导、继代增殖培养、生根培养等过程,研究筛选最适的外植体采集时间、继代及生根培养的基本培养基以及植物生长调节剂的种类及浓度配比等。【结果】10月采集的外植体诱导效果最好,存活率为32.38%;米老排腋芽诱导最适配方为MS+6-BA 0.5 mg/L+NAA 0.02 mg/L,诱导率为100%,萌发时间为4.07 d;最适增殖培养基为MX(改良WPM)+6-BA 1.0 mg/L+NAA 0.05 mg/L,增殖系数达4.94,芽生长旺盛;最适生根培养基为MX+NAA 0.3 mg/L,生根率100%,平均根数13.18,根生长健壮,移栽成活率可达92.3%。【结论】米老排外植体诱导成活率与取材地点、时间相关,广东地区10月的米老排外植体材料最佳;在使用MS基本培养基增殖系数低的情况下,改用MX(改良WPM)培养基能够显著提高米老排增殖继代倍数;本研究还对米老排诱导培养基、增殖培养基、生根培养基进行了优化,有助于建立米老排优树组培体系,有效提高组培质量和效率。

关键词：米老排基本培养基丛生芽组织培养