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Simultaneous Silencing CD40 Genes in Macrophages and Dendritic Cells Using a Novel siRNA Delivery System for Immunomodulation During Skin Transplantation

Simultaneous Silencing CD40 Genes in Macrophages and Dendrit...

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第十一届全国免疫学学术大会

作者： wang jialiang Sun Tianmeng Yang Yongguang The First Hospital and Institute of Immunology Jilin University Department of Medicine Columbia Center for Translational ImmunologyColumbia University College of Physicians and Surgeons

Objects: Develop a novel siRNA delivery system to down-regulate the expression of CD40 in macrophages and DCs in vivo and prolong the survival of allogeneic skin ***: In our study, PLGA/si CD40 nanoparticles were prepared by a double emulsion-solvent evaporation technique. An aqueous solution of siRNA(0.2 mg) in 25 μL RNase free water was emulsified by sonication for 30 s over an ice bath in 0.5 ml of chloroform containing 1.0 mg DOTAP and 25 mg m PEG5k-PLGA10 k. This primary emulsion was further emulsified in 5 m L dd H2 O by sonication for 1 min to form a water-in-oil-in-water emulsion. Then, the emulsion was moved into round-bottomed flasks to evaporate the chloroform. The PLGA/FAM-siRNA NPs were added into the medium of DC1.2 and RAW264.7 cells and the cells were collected after 48 h for flow cytometry(FCM) and confocal imaging. Furthermore, DC1.2 and RAW264.7 cells were collected for analysis of CD40 expression by quantitative PCR(qP CR) after co-cultured with PLGA/si CD40 NPs for 48 h. The C57BL/6 mice were sacrificed and PBMCs, splenocytes and bone marrow cells were collected for FCM 16 h after PLGA/Cy5-siRNA NPs i.v. injection. We evaluated the biological effects of PLGA/si CD40 NPs in vivo in PLGA NPs i.v. injection Balb/c to C57BL/6 skin graft ***: PEG-PLGA nanoparticles could deliver siRNAinto macrophages and DCs in vitro, which was demonstrated by both FCM results and confocal imaging. Also, we evaluated the efficiency of down regulation of CD40 expression by PLGA/si CD40 NPs both in vitro and in vivo. In the transient parabiosis skin transplantation model, PLGA/si CD40 NPs could prolong the survival of transplanted skin ***: PEG-PLGA nanoparticles was a proper siRNA delivery system for macrophages and DCs, which can effectively down-regulate the expression of CD40 both in vitro and in vivo, and prolonged the survival of allogeneic skin graft.

关键词： CD40 RNAi nanoparticles immunomodulation

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Mechanical properties and fracture surface roughness of thermally damaged granite under dynamic splitting

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Deep Underground Science and Engineering 2024年第1期3卷 103-116页

作者： Yijin Qian Peng Jia Songze Mao jialiang Lu School of Resources and Civil Engineering Northeastern UniversityShenyangChina

In order to understand the mechanical properties and the fracture surface roughness characteristics of thermally damaged granite under dynamic splitting,dynamic Brazilian splitting tests were conducted on granite samples after thermal treatment at 25,200,400,and 600℃.Results show that the dynamic peak splitting strength of thermally damaged granite samples increases with increasing strain rate,showing obvious strain‐rate *** increasing temperature,thermally induced cracks in granite transform from intergranular cracks to intragranular cracks,and to a transgranular crack *** induced damages reduce the dynamic peak splitting strength and the maximum absorbed energy while increasing the peak radial *** fracture mode of the thermally damaged granite under dynamic loads is mode Ⅱ splitting *** using the axial roughness index Z2 a,the distribution ranges of the wedge‐shaped failure zones and the tensile failure zones in the fracture surfaces under dynamic Brazilian splitting can be effectively *** radial roughness index Z_(2)^(r)is sensitive to the strain rate and *** shows a linear correlation with the peak splitting strength and the maximum absorbed energy and a linear negative correlation with the peak radial strain.Z_(2)^(r)can be used to quantitatively estimate the dynamic parameters based on the models proposed.

关键词： dynamic splitting fracture surface roughness granite strain rate thermal treatment

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Chiral twisted molecular carbons:Synthesis,properties,and applications

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Interdisciplinary Materials 2024年第4期3卷 453-479页

作者： Yunqin Zhang Junjie Guan Leiquan Luo Xiao Han Jie wang Yongshen Zheng jialiang Xu School of Materials Science and Engineering Smart Sensing Interdisciplinary Science CenterFrontiers Science Center for New Organic MatterNankai UniversityTianjinChina Collaborative Innovation Center of Chemical Science and Engineering TianjinChina

In recent years,the precisely controlled synthesis of chiral twisted molecular carbons has emerged as a forefront topic in the research of carbon *** carbons refer to carbon nanomaterials synthesized with precision at the atomic *** rational design,rigid and stable chiral twisted structures can be *** exploration in the field of chiral twisted molecular carbons is key to fully understanding the various twisted configurations of carbon materials and delving into the relationship between structure design and *** review explores chiral twisted configurations of carbon nanomater-ials such as nanographene,carbon nanobelts,carbon nanosheets,graphdiyne,*** emphasizes the role of photocyclization,Scholl reaction,and Diels–Alder reactions in achieving precise chiral control and discusses a range of innovative design *** strategies have led to the development of various twisted structures,such as helical,propeller,and Möbius strip *** introduction of chirality,combined with the inherent exceptional optical properties of nanocarbon materials,has facilitated the creation of materials with superior chiroptical *** advancement is driving applications in fields such as optoelectronics and chiral optics.

关键词： carbon nanomaterials carbon nanobelts chirality molecular carbon nanographene

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The Design and Implement of TCP/IP Protocol Cluster on AVR Singlechip

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Journal of Software Engineering and Applications 2010年第8期3卷 761-766页

作者： Rong Pan Hai Zhao jialiang wang Dan Liu Penghua Cai 不详

With the rapid development of the embedded technology, research and implement of the Internet of things will be a new technology revolution, yet the implement of the Internet of things is on the base of the communication between the things. For this reason, realizing the function of communication between singlechip is particularly important. Based on the characteristics of the embedded microcontroller, we analyzed the traditional PC TCP/IP protocol, and appropriately tailored TCP/IP protocol cluster on the basis of the characteristics of embedded singlechip. At last, we realized the reduced TCP/IP protocol cluster suitable for embedded singlechip, on AVR singlechip platform.

关键词： The Internet of Things Webit Embedded System TCP/IP Protocol Ethernet

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Analysis and Comparison of Five Kinds of Typical Device-Level Embedded Operating Systems

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Journal of Software Engineering and Applications 2010年第1期3卷 81-90页

作者： jialiang wang Hai ZHAO Peng LI Hui LI Bo LI 不详

Today, the number of embedded system was applied in the field of automation and control has far exceeded a variety of general-purpose computer. Embedded system is gradually penetrated into all fields of human society, and ubiquitous embedded applications constitute the 'ubiquitous' computing era. Embedded operating system is the core of the em-bedded system, and it directly affects the performance of the whole system. Our Liaoning Provincial Key Laboratory of Embedded Technology has successfully developed five kinds of device-level embedded operating systems by more than ten years’ efforts, and these systems are Webit 5.0, Worix, μKernel, iDCX 128 and μc/os-II 128. This paper mainly analyses and compares the implementation mechanism and performance of these five kinds of device-level embedded operating systems in detail.

关键词： Embedded System Operating System Core Real-Time Ability I/O Delay and Jitter Pervasive Computing and Internet of Things

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Web Layout Design of Large Cavity Structures Based on Topology Optimization

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Computer Modeling in Engineering & Sciences 2024年第3期138卷 2665-2689页

作者： Xiaoqiao Yang jialiang Sun Dongping Jin State Key Laboratory ofMechanics and Control for Aerospace Structures College of Aerospace EngineeringNanjing University of Aeronautics and AstronauticsNanjing210016China

Large cavity structures are widely employed in aerospace engineering, such as thin-walled cylinders, blades andwings. Enhancing performance of aerial vehicles while reducing manufacturing costs and fuel consumptionhas become a focal point for contemporary researchers. Therefore, this paper aims to investigate the topologyoptimization of large cavity structures as a means to enhance their performance, safety, and efficiency. By usingthe variable density method, lightweight design is achieved without compromising structural strength. Theoptimization model considers both concentrated and distributed loads, and utilizes techniques like sensitivityfiltering and projection to obtain a robust optimized configuration. The mechanical properties are checked bycomparing the stress distribution and displacement of the unoptimized and optimized structures under the sameload. The results confirm that the optimized structures exhibit improved mechanical properties, thus offering keyinsights for engineering lightweight, high-strength large cavity structures.

关键词： Topology optimization lightweight design web layout design cavity structure

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基于打滑地层的孕镶金刚石钻头设计

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地球科学（中国地质大学学报） 2016年第5期41卷 895-900页

作者：王佳亮张绍和湖南科技大学海洋矿产资源探采装备与技术湖南省工程实验室湖南湘潭411201 中南大学地球科学与信息物理学院湖南长沙410083

为了提高金刚石钻头在坚硬致密、弱研磨性地层的钻进效率,将弱化胎体耐磨损性能理论与切削齿非光滑设计相结合,制作钻头进行现场试验并对胎体进行扫描电镜分析.研究结果表明:在胎体中添加适当浓度的胎体弱化颗粒,有利于提高钻头的钻速;... 详细信息

为了提高金刚石钻头在坚硬致密、弱研磨性地层的钻进效率,将弱化胎体耐磨损性能理论与切削齿非光滑设计相结合,制作钻头进行现场试验并对胎体进行扫描电镜分析.研究结果表明:在胎体中添加适当浓度的胎体弱化颗粒,有利于提高钻头的钻速;经胎体耐磨性弱化处理的钻头在钻进过程中,弱化颗粒易于从胎体表面脱落,使其表面形成微观非光滑形态,提高了钻头唇面与岩石的单位面积压力,增加了孔底岩粉的研磨能力,促进了胎体中新颗粒金刚石的出刃;弱化颗粒浓度存在一个较优的设计范围,过高或过低都不利于提高钻头的钻进效率和使用寿命.

关键词：胎体耐磨损性弱化金刚石钻头非光滑形态胎体弱化颗粒弱化颗粒浓度工程地质

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论小说形态更新与中国文学的现代转型——以越文化视阈内的小说变革为例

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天津社会科学 2020年第6期 106-113页

作者：王嘉良浙江师范大学人文学院暨江南文化研究中心

探讨中国文学的现代转型,小说领域突破传统观念的桎梏,实现小说形态的根本性转换是一个重要研究视角。越文化的深厚积淀,崛起了由鲁迅领衔的新文学作家群,引领中国现代小说变革潮流,为文学转型提供了范例。透过越地作家随着现代小说意... 详细信息

探讨中国文学的现代转型,小说领域突破传统观念的桎梏,实现小说形态的根本性转换是一个重要研究视角。越文化的深厚积淀,崛起了由鲁迅领衔的新文学作家群,引领中国现代小说变革潮流,为文学转型提供了范例。透过越地作家随着现代小说意义的被发现、被充分演绎而逐层推进的小说内涵更新,及其汲取外来文艺思潮造就小说形态创新,当能从小说形态转型视角确切阐释文学转型的必然性、可能性及其有效路径。

关键词：小说新变内涵提升形态转换文学转型

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“先锋与常态”:建构新的文学阐释模式的需要和可能

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中国现代文学研究丛刊 2006年第6期 274-281页

作者：王嘉良浙江师范大学人文学院 321004

陈思和先生在北大的讲演《“五四”文学：在先锋性与大众化之间》发表以后，已引起学界的关注。这个讲演涉及建构新的现代文学阐释模式的需要和可能，已构成对旧有模式不小的冲击；尽管这个模式的建构还在探讨之中，它还可以进一步完善... 详细信息

陈思和先生在北大的讲演《“五四”文学：在先锋性与大众化之间》发表以后，已引起学界的关注。这个讲演涉及建构新的现代文学阐释模式的需要和可能，已构成对旧有模式不小的冲击；尽管这个模式的建构还在探讨之中，它还可以进一步完善，但它肯定会对日后的现代文学研究产生深刻影响。

关键词： “五四”文学阐释模式现代文学研究常态大众化先锋性陈思和讲演

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中国文学现代转型的“内源性”生成机制探析——从越文化视阈的透视

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文学评论 2022年第3期 90-97页

作者：王嘉良浙江师范大学人文学院、江南文化研究中心

中国文学的现代转型中,对世界文化新潮的有效汲取固然不可或缺,但“传统内的变化”也是一个重要因素。越文化“近传统”蕴含的叛逆道统、注重变革的精神,曾长期影响我国的思想文化界;越地的面海区位优势,养成作家外向拓展的精神品性,造... 详细信息

中国文学的现代转型中,对世界文化新潮的有效汲取固然不可或缺,但“传统内的变化”也是一个重要因素。越文化“近传统”蕴含的叛逆道统、注重变革的精神,曾长期影响我国的思想文化界;越地的面海区位优势,养成作家外向拓展的精神品性,造就内外共振的文化效应;而越文化在传统格局中的新变,也形成一种历史流转的文化精神。这些特征,恰好与越地作家群体开创中国新文学的卓越建树形成同构对应关系。由此可以看出:越文化是中国文学现代转型的一种重要“内源性”机制。

关键词：越文化近传统小传统新变精神

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