检索结果-南通市图书馆

China CDC weekly 2024年第8期6卷 143-147页

作者： jiali cao Jie Pan Xiaoqing Yang Jumei Liu Min Zhu Zeyu Zhao Ling Chen Tianmu Chen Huiming Ye Department of Laboratory Medicine Fujian Key Clinical Specialty of Laboratory MedicineWomen and Children’s HospitalSchool of MedicineXiamen UniversityXiamen CityFujian ProvinceChina State Key Laboratory of Vaccines for Infectious Diseases Xiang An Biomedicine LaboratorySchool of Public HealthXiamen UniversityXiamen CityFujian ProvinceChina Department of Pediatrics Women and Children's HospitalSchool of MedicineXiamen UniversityXiamen CityFujian ProvinceChina

Summary What is already known about this topic?Respiratory infections pose a significant burden on public health.Despite recent outbreaks occurring in various locations,there is limited information available on the pr... 详细信息

关键词： Xiamen spite Fujian

The appearance of cytoplasmic cytochrome C precedes apoptosis during Drosophila salivary gland degradation

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Insect Science 2024年第1期31卷 157-172页

作者： Shihui Long Wenxin cao Yongyu Qiu Ruohan Deng jiali Liu Lidan Zhang Renke Dong Fengxin Liu Sheng Li Haigang Zhao Na Li Kang Li Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied Technology Institute of Insect Science and TechnologySchool of Life SciencesSouth China Normal UniversityGuangzhouChina Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhouChina Guangmeiyuan R&D Center Guangdong Provincial Key Laboratory of Insect Developmental Biology and Applied TechnologySouth China Normal UniversityMeizhouGuangdong ProvinceChina School of Food Science and Engineering South China University of TechnologyGuangzhouChina ChemPartner PharmaTech Co. LtdJiangmenGuangdong ProvinceChina

Apoptosis is an important process for organism development that functions to eliminate cell damage,maintain homeostasis,and remove obsolete tissues during morphogenesis.In mammals,apoptosis is accompanied by the release of cytochrome C(Cyt-c)from mitochondria to the cytoplasm.However,whether this process is conserved in the fruit fly,Drosophila melanogaster,remains controversial.In this study,we discovered that during the degradation of Drosophila salivary gland,the transcription of mitochondria apoptosis factors(MAPFs),Cyt-c,and death-associated APAF1-related killer(Dark)encoding genes are all upregulated antecedent to initiator and effector caspases encoding genes.The proteins Cyt-c and the active caspase 3 appear gradually in the cytoplasm during salivary gland degradation.Meanwhile,the Cyt-c protein colocates with mito-GFP,the marker indicating cytoplasmic mitochondria,and the change in mitochondrial membrane potential coincides with the appearance of Cyt-c in the cytoplasm.Moreover,impeding or promoting 20E-induced transcription factor E93 suppresses or enhances the staining of Cyt-c and the active caspase 3 in the cytoplasm of salivary gland,and accordingly decreases or increases the mitochondrial membrane potential,respectively.Our research provides evidence that cytoplasmic Cyt-c appears before apoptosis during Drosophila salivary gland degradation,shedding light on partial conserved mechanism in apoptosis between insects and mammals.

关键词： active caspase 3 apoptosis Cyt-c Drosophila salivary gland

Effects of grain boundary diffusion process on magnetic properties enhancement and microstructure evolution of hot-deformed Nd-Fe-B magnets

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Rare Earths 2023年第5期41卷 698-704,I0003页

作者： Jiayi He Yukun Liu Zhigao Yu jiali cao Hongya Yu Zhongwu Liu School of Materials Science and Engineering South China University of TechnologyGuangzhou 510640China

Grain boundary diffusion(GBD)process is an important approach for producing Nd-Fe-B magnets with high coercivity and high thermal stability.The GBD for hot-deformed Nd-Fe-B magnets with nanocrystalline micro structure is more complicated compared to sintered magnets.Here,we investigated the effects of different GBD methods,i.e.,intergranular addition(in-situ GBD 1#),in-situ GBD from magnet surface during hot pressing and hot deformation(in-situ GBD 2#),and conventional GBD,on the magnetic properties and microstructure of hot deformed magnets.After the treatment by these three GBD approaches using 2 wt%Pr_(40)Tb_(30)Cu_(30)diffusion source,the coercivity of the hot-deformed magnet increases from 1281 to 1567,1412 and 2022 kA/m,respectively.The coercivity enhancement is attributed to the formation of local(Nd,Tb)2Fe14B phase with stro ng magnetic anisotropy.Reduced grain orientation is found in both in-situ GBD 1#and conventional GBD treated samples mainly due to the local stress state variation and the rotation of platelet grains.Interestingly,the in-situ GBD 2#processed sample has a high orientation at diffusion surface,which may be caused by the modified surface state of the magnet by the diffusion source.Compared with the in-situ GBD processes,the conventional GBD exhibits a higher utilization efficiency of Tb.Since the in-situ GBD is effective to treat thick hot-deformed magnets,further effort should be aimed at enhancing its diffusion efficiency.

关键词： Nd-Fe-B Grain boundary diffusion Coercivity hot deformation Crystal orientatio

Can Tibetan medicine Honghua Ruyi pills relieve endometriosisassociated dysmenorrhea?Protocol for a randomized placebocontrolled trial

在线全文

学校读者我要写书评

暂无评论

Journal of Traditional Chinese Medical Sciences 2024年第1期11卷 78-85页

作者： Mei Han Jiahui cao jiali Wei Hui Luo Chaoqin Yu Xuefang Liang Nyangmotse Guoyan Yang Huilan Du Jianping Liu Centre for Evidence-Based Chinese Medicine Beijing University of Chinese MedicineBeijing 102488China College of Management Beijing University of Chinese MedicineBeijing 102488China College of Acupuncture and Moxibustion Beijing University of Chinese MedicineBeijing 102488China Institute for Tibetan Medicine China Tibetology Research CenterBeijing 100101China Changhai Hospital Naval Medical UniversityShanghai 200433China Department of Gynaecology Guangdong Provincial Hospital of Chinese MedicineGuangdong 510120China Department of Gynaecology Tibetan Medicine Hospital of Qinghai ProvinceXining 810007China NICM Health Research Institute Western Sydney UniversityPenrithNSW 2751Australia College of Integrated Chinese and Western Medicine Hebei University of Chinese MedicineShijiazhuang 050200China

Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 ratio.The primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule size.The safety was monitored throughout the treatment period.All the analyses were based on the intention-to-treat principle.For continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of occurrences.Differences were compared using the chi-square test or Fisher's exact test.The predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment allocation.Estimates of treatment effects were reported with 95%confidence intervals.Two-tailed P values≤.05 were considered statistically significant.Conclusion:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.

关键词： Endometriosis Dysmenorrhea Honghua Ruyi pills Randomized controlled trial Traditional Chinese medicine

Rhizoma Drynariae-derived nanovesicles reverse osteoporosis by potentiating osteogenic differentiation of human bone marrow mesenchymal stem cells via targeting ERα signaling

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Acta Pharmaceutica Sinica B 2024年第5期14卷 2210-2227页

作者： Qing Zhao Junjie Feng Fubin Liu Qianxin Liang Manlin Xie Jiaming Dong Yanfang Zou jiali Ye Guilong Liu Yue cao Zhaodi Guo Hongzhi Qiao Lei Zheng Kewei Zhao Guangzhou Key Laboratory of Chinese Medicine Research on Prevention and Treatment of Osteoporosis the Third Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhou 510378China Guangdong Engineering Research Center of Chinese Herbal-derived Vesicles Guangzhou University of Chinese MedicineGuangzhou 510006China Department of Laboratory Medicine Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China The Fifth Affiliated Hospital Sun Yat-sen University Zhuhai 519000China Department of Blood Transfusion Guangdong Heyou International HospitalFoshan 528306China School of Pharmacy Nanjing University of Chinese MedicineNanjing 210023China

Although various anti-osteoporosis drugs are available,the limitations of these therapies,including drug resistance and collateral responses,require the development of novel anti-osteoporosis agents.Rhizoma Drynariae displays a promising anti-osteoporosis effect,while the effective component and mechanism remain unclear.Here,we revealed the therapeutic potential of Rhizoma Drynariae-derived nanovesicles(RDNVs)for postmenopausal osteoporosis and demonstrated that RDNVs potentiated osteogenic differentiation of human bone marrow mesenchymal stem cells(hBMSCs)by targeting estrogen receptor-alpha(ERα).RDNVs,a natural product isolated from fresh Rhizoma Drynariae root juice by differential ultracentrifugation,exhibited potent bone tissue-targeting activity and anti-osteoporosis efficacy in an ovariectomized mouse model.RDNVs,effectively internalized by hBMSCs,enhanced proliferation and ERαexpression levels of hBMSC,and promoted osteogenic differentiation and bone formation.Mechanistically,via the ERαsignaling pathway,RDNVs facilitated mRNA and protein expression of bone morphogenetic protein 2 and runt-related transcription factor 2 in hBMSCs,which are involved in regulating osteogenic differentiation.Further analysis revealed that naringin,existing in RDNVs,was the active component targeting ERαin the osteogenic effect.Taken together,our study identified that naringin in RDNVs displays exciting bone tissue-targeting activity to reverse osteoporosis by promoting hBMSCs proliferation and osteogenic differentiation through estrogen-like effects.

关键词： Rhizoma Drynariae-derived nanovesicles Bone marrow mesenchymal stem cells Bone targeting Osteogenic differentiation ERαsignaling Naringin Bone morphogenetic protein 2 Runt-related transcription factor 2

米曲霉菌发酵豆渣制备饼干的工艺研究与品质分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

大豆科学 2024年第2期43卷 217-225页

作者：薛伟顾佳丽王苏月曹仲文南京航空航天大学经济与管理学院江苏南京211100 江苏旅游职业学院烹饪科技学院江苏扬州225127 扬州大学旅游烹饪学院江苏扬州225127

为充分利用豆渣并适应人们营养健康需求,本试验将以米曲霉菌发酵后的豆渣制成富含膳食纤维的豆渣饼干。通过单因素试验探讨豆渣、起酥油、白砂糖、脱脂牛奶对豆渣饼干食品感官的影响,在此基础上进行响应面分析,优化豆渣饼干的配方。结... 详细信息

为充分利用豆渣并适应人们营养健康需求,本试验将以米曲霉菌发酵后的豆渣制成富含膳食纤维的豆渣饼干。通过单因素试验探讨豆渣、起酥油、白砂糖、脱脂牛奶对豆渣饼干食品感官的影响,在此基础上进行响应面分析,优化豆渣饼干的配方。结果表明:发酵式豆渣饼干的最佳配方(以烘焙百分比表示)为豆渣添加量31%,起酥油添加量41%,脱脂牛奶添加量36%,白砂糖添加量21%。按照此配方生产出的豆渣饼干色泽金黄,酥脆适中,甜度适宜,硬度为33.75 N,内聚性为0.25 Ratio,弹性为0.45 mm,咀嚼性为3.74 Jm,与市场畅销饼干的客观数据接近。各理化指标符合国家标准,发酵豆渣饼干水分含量为3.60%,灰分含量为1.30%,水分活度为0.73,粗脂肪含量为26.00 g·(100 g)^(-1),粗蛋白含量为10.63 g·(100 g)^(-1),膳食纤维含量为6.24 g·(100 g)^(-1)。结果为制备出具良好的市场前景的富含膳食纤维的豆渣饼干奠定基础。

关键词：大豆豆渣米曲霉菌发酵响应面分析质构食品感官饼干

水电用Q690CFD低合金高强钢SH⁃CCT曲线测定与分析

在线全文

学校读者我要写书评

暂无评论

金属热处理 2024年第2期49卷 60-65页

作者：曹佳丽靳红泽李康立徐亚鹏赵强王翠萍李梦楠国网新源控股有限公司抽水蓄能技术经济研究院北京100053 水利部水工金属结构质量检验测试中心河南郑州450044

使用Gleeble⁃3800热模拟试验机建立了水电用Q690CFD高强钢的SH⁃CCT曲线,研究了冷却速度对试验钢粗晶区显微组织与硬度的影响。结果表明,在所测试冷却速度下,试验钢热影响区出现了马氏体、马氏体+贝氏体与贝氏体3种转变形式。其中当冷却... 详细信息

使用Gleeble⁃3800热模拟试验机建立了水电用Q690CFD高强钢的SH⁃CCT曲线,研究了冷却速度对试验钢粗晶区显微组织与硬度的影响。结果表明,在所测试冷却速度下,试验钢热影响区出现了马氏体、马氏体+贝氏体与贝氏体3种转变形式。其中当冷却速度为40℃/s时,试验钢热影响区硬度达到最大,为344 HV10,组织为板条马氏体与贝氏体铁素体的混合组织。随着冷却速度的降低,热影响区组织中开始出现粒状贝氏体,基体硬度呈现逐渐减小的趋势,同时贝氏体内部的M⁃A组元也由颗粒状与短棒状转变为片状。当冷却速度低于5℃/s时,热影响区组织全部为粒状贝氏体,且基体硬度出现了低于母材的现象。

关键词：低合金高强钢热模拟相变温度 SH⁃CCT曲线

在线全文

学校读者我要写书评

暂无评论

采收期对烟富3号苹果果实品质的影响

中国果树 2024年第6期 26-32页

作者：杨喜盟张彪李嘉立曹亚凤平凉市农业科学院

以15年生烟富3号苹果为试材，研究了甘肃省陇东旱塬区6个采收期（2022年10月5日、10月11日、10月17日、10月23日、10月29日、11月5日）果实低温贮藏时品质生理变化规律及特性。结果表明：随着采收期的推迟，果形指数无显著变化，果实纵... 详细信息

以15年生烟富3号苹果为试材，研究了甘肃省陇东旱塬区6个采收期（2022年10月5日、10月11日、10月17日、10月23日、10月29日、11月5日）果实低温贮藏时品质生理变化规律及特性。结果表明：随着采收期的推迟，果形指数无显著变化，果实纵径、横径、可溶性固形物含量、可溶性糖含量及固酸比、糖酸比、糖心率均显著增加，硬度及可滴定酸含量、总酚含量显著降低，10月23日采收的果实单果重最大、着色最好、维生素C含量最高，可溶性固形物含量达15.13%。低温贮藏期间，不同采收期果实硬度及可滴定酸含量、维生素C含量均不断下降，可溶性固形物含量先升高后下降，总酚含量先下降后升高，果实呼吸强度、乙烯释放速率均在贮藏120 d时出现峰值；10月17日采收的果实硬度及可滴定酸含量、维生素C含量下降幅度较小，果面光洁度保持稳定，贮藏180 d后，果实硬度、可溶性固形物含量、维生素C含量、L*值均最高，果实呼吸强度、乙烯释放速率峰值、失重率、腐烂率均最低。综合分析，10月23日可作为甘肃省陇东旱塬区烟富3号果实鲜食的最适宜采收期，10月17日可作为长期低温贮藏的最佳采收期。

关键词：陇东旱塬烟富3号苹果采收期果实品质贮藏性

敲除LTA4H基因的PK-15细胞系构建及其对口蹄疫病毒复制的影响

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

微生物学报 2024年第3期64卷 733-744页

作者：王家丽蒲秀瑛杨帆邵文华黄梦瑶曹伟军郑海学张伟兰州理工大学生命科学与工程学院甘肃兰州730050 中国农业科学院兰州兽医研究所/兰州大学动物医学与生物安全学院动物疫病防控全国重点实验室甘肃兰州730000 甘肃省病原生物学基础学科研究中心甘肃兰州730046

【目的】利用CRISPR/Cas9基因编辑技术构建白三烯A4水解酶(leukotriene A4 hydrolase,LTA4H)基因缺失的猪肾细胞系(porcine kidney-15,PK-15),探究LTA4H对口蹄疫病毒(foot-and-mouth disease virus,FMDV)复制的影响,为开展LTA4H功能研究及调控病毒复制机制研究提供理论依据。【方法】设计2条针对猪LTA4H基因的引导RNA(small guide RNA,sgRNA),分别构建至载体pX459-puro-MCS中;将CRISPR重组质粒转染PK-15细胞,用嘌呤霉素(puromycin)抗生素筛选,并通过有限稀释法筛选单克隆细胞,之后通过Western blotting和测序检测LTA4H基因的敲除,获得LTA4H基因功能缺失细胞系。使用Western blotting、RT-qPCR及病毒滴度测定等方法检测敲除LTA4H基因后对FMDV复制及相关蛋白的表达情况。【结果】获得的单克隆敲除细胞系与野生型细胞相比,能够显著抑制FMDV复制。【结论】本研究成功构建了LTA4H基因敲除的PK-15细胞系,证明LTA4H对FMDV的复制具有促进作用,研究结果为后续LTA4H功能研究提供理论依据。

关键词： CRISPR/Cas9 白三烯A4水解酶细胞系口蹄疫病毒

PD-1单抗联合顺铂或吉西他滨化疗对KRAS突变非小细胞肺癌A549细胞移植瘤小鼠模型的治疗作用

在线全文

学校读者我要写书评

暂无评论

中国肿瘤生物治疗杂志 2024年第4期31卷 371-376页

作者：李雄兵周瑞芬李佳丽王汉姣王超李婧曹喆舒诚荣咸宁市中心医院肿瘤内科湖北咸宁437000

目的:探讨程序性死亡受体-1(PD-1)单抗联合顺铂或吉西他滨在KRAS基因突变非小细胞肺癌(NSCLC)A549细胞移植瘤小鼠模型治疗中的作用。方法:构建免疫系统-肿瘤双人源化A549细胞小鼠移植瘤模型,将60只小鼠按随机数字表法分成6组(10只/组),... 详细信息

目的:探讨程序性死亡受体-1(PD-1)单抗联合顺铂或吉西他滨在KRAS基因突变非小细胞肺癌(NSCLC)A549细胞移植瘤小鼠模型治疗中的作用。方法:构建免疫系统-肿瘤双人源化A549细胞小鼠移植瘤模型,将60只小鼠按随机数字表法分成6组(10只/组),分别为对照组(200μL/kg PBS)、PD-1单抗组(20 mg/kg PD-1单抗)、顺铂组(3 mg/kg顺铂)、PD-1单抗+顺铂组(20 mg/kg PD-1单抗+3 mg/kg顺铂)、吉西他滨组(30 mg/kg吉西他滨)和PD-1单抗+吉西他滨组(20 mg/kg PD-1单抗+30 mg/kg吉西他滨)。TUNEL和DAPI双染色法检测移植瘤组织中细胞凋亡水平,测量移植瘤体积和质量并计算肿瘤生长抑制率,免疫组化法检测移植瘤微血管密度(MVD)。结果:成功构建免疫系统-肿瘤双人源化NSCLC A549细胞小鼠移植瘤模型,PD-1单抗+顺铂组移植瘤的细胞凋亡率、肿瘤生长抑制率均最高,移植瘤体积、质量和MVD均最小,与其他5组小鼠比较差异均有统计学意义(均P<0.05)。结论:顺铂与PD-1单抗具有协同活性,而吉西他滨拮抗PD-1单抗的治疗作用。提示PD-1单抗联合顺铂对KRAS突变NSCLC A549细胞移植瘤小鼠的疗效更好。

关键词： PD-1单抗顺铂吉西他滨非小细胞肺癌 KRAS突变免疫治疗