检索结果-南通市图书馆

Trilobatin Acts as a Marker Metabolite Involved in Flavonoid Accumulation Regulated by CsWRKY28-MYC2 with Trypsin Activation in Cucumber (Cucumis sativus)

引用

Phyton-International Journal of Experimental Botany 2024年第11期93卷 2837-2856页

作者： Enyan Chen Jingyu Jia jiaju sun Jie Wang Xinxin Chen Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Chinese Academy of Agricultural Sciences Cotton Research Institute Anyang455000China The National and Local Joint Engineering Laboratory of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South Xinjiang Tarim UniversityAlar843300China National Demonstration Center for Experimental Food Processing and Safety Education Luoyang471000China

During post-harvest storage of Cucumis sativus fruit,the application of trypsin treatment could increaseﬂavonoid compound levels and reduce oxidative *** investigate the mechanism of trypsin-inducedﬂavonoid bio-synthesis in ***,we conducted a combined analysis of transcriptomics and widely targeted *** hundred and seventy-ﬁve signiﬁcantly different metabolites were obtained from metabolomics *** kyoto encyclopedia of genes and genomes(KEGG)functional enrichment results indicated that these metabolites were mainly involved in the phenylpropanoid biosynthesis *** combining the results of the weighted gene co-expression network analysis(WGCNA)with the 130 upregulated phenylpropanoid metabolites,22 signiﬁcantly upregulated phenylpropanoid metabolites were identiﬁ*** was identiﬁed as the most prominent meta-bolite through cluster analysis and variable importance in projection(VIP)*** performance liquid chro-matography(HPLC)experiments conﬁrmed that trilobatin was the key metabolite induced by *** transcriptomic results showed that 1068 genes in the brown module of WGCNA were highly positively correlated withﬂavonoid *** gene set enrichment analysis(GSEA)identiﬁed leading edges in 4 key KEGG ***,combined with WGCNA and GSEA analysis results,35 core genes were *** co-expression network of transcriptomics and metabolomics suggested that CsWRKY28 and CsMYC2 regulated the biosynthesis of *** quantitative real-time polymerase chain reaction(RT-qPCR)and dual luciferase experiments con-ﬁrmed the activation effect of CsWRKY28 on CsMYC2 and downstream target *** study revealed the key transcription factors involved in the trypsin-controlled biosynthesis of trilobatin in *** and provided a new theoretical basis for elucidating the molecular mechanism of trypsin preservation.

关键词： CsWRKY28 transcriptomics trilobatin trypsin widely targeted metabolomics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

4-Hydroxy-2-Oxoglutaric Acid,A KeyMetabolite Involved in Trypsin-Regulation of Arginine Metabolism in Hylocereus undatus during Storage

引用

Phyton-International Journal of Experimental Botany 2024年第5期93卷 885-900页

作者： Bairu Li Jingyu Jia Hemin Wang jiaju sun Enyan Chen Xin Li College of Food and Bioengineering Henan University of Science and TechnologyLuoyang471023China Henan Engineering Research Center of Food Microbiology Luoyang471023China National Demonstration Center for Experimental Food Processing and Safety Education Luoyang471000China

Trypsin,a novel superoxide scavenger,significantly enhances the storage quality of Hylocereus undatus(***).To elucidate the preservation mechanism of trypsin on ***,a widely targeted metabolomic analysis,and transcriptomics analysis were ***,a total of 453 metabolites were identified,with organic acids and their derivatives constituting the largest proportion(25%).Amino acids and their metabolites,prominent among organic acids,were further *** them,73 metabolites were associated with amino acids,and 37 exhibited significant *** most enriched Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway was arginine biosynthesis(map00220),with polyamine metabolites showing the most pronounced differences,particularly spermine(FC=1.7594).Compared with the control group,4-hydroxy-2-oxoglutaric acid was significantly upregulated(FC=2.117)in the process of spermine ***,the results of Gene Ontology(GO)and KEGG enrichment analysis of the *** transcriptome profile revealed that trypsin treatment led to 187 differentially expressed genes associated with *** GO and KEGG analyses exhibited significant enrichment in the spermine biosynthetic process(GO:0006597)(map:00220)within the arginine biosynthesis ***,most enzymes and metabolites within the spermine biosynthesis pathway in *** were *** results of the PPI network highlighted that ADC,SPDS,and SAMDC,among others,were pivotal proteins involved in trypsin-regulated arginine metabolism and spermine *** study revealed that trypsin could significantly delay postharvest senescence of *** at room *** effect might be attributed to trypsin triggering the synthesis of 4-hydroxy-2-oxoglutaric acid in the fruit peel,thereby promoting the biosynthesis of spermine and other polyamines.

关键词： Trypsin arginine transcriptomics widely targeted metabolomics

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

NEW MECHANICAL MODEL OF HEVERSIAN BONE

引用

Chinese Science Bulletin 1985年第12期30卷 1595-1596页

作者：孙家驹 Northwestern Institute of Architectural Engineering Xi'an

Many researchers are interested in the structure of compact bone of human. Ascenzi and Bonucci isolated the single osteon, measured the Young’s modulus in tension and compression, and observed the shape of osteons an... 详细信息

关键词： interested modulus profound appearing walled

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

全球气温上升的原因及其后果

引用

江西行政学院学报 2006年第4期8卷 89-92页

作者：孙家驹中共江西省委党校江西南昌330003

气候变化的原因极为复杂,科学家在经过长时间的犹豫后,终于确认由于人为原因而造成了当前的全球气温上升,这一过程已经不可逆转,这将导致复杂的链式反应,整个生态系统将可能发生替换,人类可能面临巨大的麻烦。

关键词：气温上升人为原因链式反应

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中深孔爆破成井技术在某矿的应用研究

引用

中国矿山工程 2019年第1期48卷 68-71页

作者：孙家驹中国恩菲工程技术有限公司北京100038

切割井是中深孔爆破落矿中一项重要的切割工程,中深孔爆破成井技术是安全、高效施工切割井方法之一。本文分析中深孔爆破成井中的技术关键点,对中深孔爆破成井技术进行研究、试验,通过实践证明该技术能够代替普通法施工切割井,并能取得... 详细信息

切割井是中深孔爆破落矿中一项重要的切割工程,中深孔爆破成井技术是安全、高效施工切割井方法之一。本文分析中深孔爆破成井中的技术关键点,对中深孔爆破成井技术进行研究、试验,通过实践证明该技术能够代替普通法施工切割井,并能取得一定经济效益和社会效益,具有推广价值。

关键词：中深孔爆破成井补偿空间切割井

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

消失模铸造管件产生“薄壁”缺陷的对策措施

引用

特种铸造及有色合金 2006年第4期26卷 238-239页

作者：孙家聚袁东洲高修启延建军山西省阳城县华王通用离心铸管厂陕西省内燃机配件二厂

分析了干砂负压消失模铸造生产铸铁管件中出现的“薄壁”发现现象,发现缺陷产生的部位,主要发生在铁水充型的最后部位,产生的原因是金属液补充不及时和泡沫模遇热分解气体排逸不顺畅造成的。为此采取提高耐火材料的耐火度,增大硅砂的粒... 详细信息

分析了干砂负压消失模铸造生产铸铁管件中出现的“薄壁”发现现象,发现缺陷产生的部位,主要发生在铁水充型的最后部位,产生的原因是金属液补充不及时和泡沫模遇热分解气体排逸不顺畅造成的。为此采取提高耐火材料的耐火度,增大硅砂的粒度,合理控制浇注速度等方法,基本消除了“薄壁”缺陷,生产出了合格的管件,工艺出品率显著提高,提升了出口铸件的品质等级,取得良好的经济效益。

关键词：消失模铸造薄壁缺陷管件

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

小学英语O2O课程资源开发与应用研究

引用

英语教师 2020年第16期20卷 32-36页

作者：孙嘉琚天津市南开区教育中心天津300074

基于国家、地方教材及学情,以学生学习兴趣为突破口,不断开发适合师生教与学的各类教学资源,巧妙利用线上和线下课程资源,指导教师有效运用O2O课程资源进行教学,以提高教学质量,促进学生英语学科核心素养的培育。O2O课程资源的开发与应... 详细信息

基于国家、地方教材及学情,以学生学习兴趣为突破口,不断开发适合师生教与学的各类教学资源,巧妙利用线上和线下课程资源,指导教师有效运用O2O课程资源进行教学,以提高教学质量,促进学生英语学科核心素养的培育。O2O课程资源的开发与应用也进一步提高了教师的专业能力。

关键词： O2O教学模式小学英语课程资源

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

中国古代木结构有限元动力分析

引用

土木工程学报 2000年第1期33卷 32-35页

作者：赵均海俞茂宏杨松岩孙家驹西北建筑工程学院陕西西安市710061 西安交通大学南洋理工大学

通过对我国古建筑木结构的结构特性研究，提出了将榫卯和斗拱简化为变刚性连接，用三维动力弹塑性有限元分析，得出了我国古建筑木结构的典型代表西安东门城楼的固有频率、振型。在ElCentro波作用下分析给出了轴力、加速度和位移等的时... 详细信息

通过对我国古建筑木结构的结构特性研究，提出了将榫卯和斗拱简化为变刚性连接，用三维动力弹塑性有限元分析，得出了我国古建筑木结构的典型代表西安东门城楼的固有频率、振型。在ElCentro波作用下分析给出了轴力、加速度和位移等的时程响应曲线，讨论了变刚度单元的刚度系数的影响，为古建筑研究提供了一种新的分析模型。

关键词：木结构变刚性连接动力有限元结构特性古建筑

来源：

维普期刊数据库

同方期刊数据库评论