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Mechanics of water pore formation in lipid membrane under electric field

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Acta Mechanica Sinica 2017年第2期33卷 234-242页

作者： Bing Bu Dechang Li jiajie diao Baohua Ji Biomechanics and Biomaterials Laboratory Department of Applied MechanicsBeijing Institute of Technology Department of Cancer Biology College of MedicineUniversity of Cincinnati

Transmembrane water pores are crucial for substance transport through cell membranes via membrane fusion, such as in neural communication. However, the molecular mechanism of water pore formation is not clear. In this study, we apply all-atom molecular dynamics and bias-exchange metadynamics simulations to study the process of water pore formation under an electric field. We show that water molecules can enter a membrane under an electric field and form a water pore of a few nanometers in diameter. These water molecules disturb the interactions between lipid head groups and the ordered arrangement of lipids. Following the movement of water molecules, the lipid head groups are rotated and driven into the hydrophobic region of the membrane. The reorientated lipid head groups inside the membrane form a hydrophilic surface of the water pore. This study reveals the atomic details of how an electric field influences the movement of water molecules and lipid head groups, resulting in water pore formation.

关键词： Cell membrane Membrane fusion Water pore formation Electric field Molecular dynamics simulation

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Lipid species dependent vesicles clustering caused by alpha-synuclein as revealed by single-vesicle imaging with total internal reflection fluorescence microscopy

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Biophysics Reports 2021年第6期7卷 437-448页

作者： Chinta Mani Aryal Owen Tyoe jiajie diao Department of Cancer Biology University of Cincinnati College of Medicine Department of Physics University of Cincinnati

Single-molecule methods have been applied to study the mechanisms of many biophysical systems that occur on the nanometer scale. To probe the dynamics of such systems including vesicle docking, tethering, fusion, trafficking, protein-membrane interactions, etc., and to obtain reproducible experimental data; proper methodology and framework are crucial. Here, we address this need by developing a protocol for immobilization of vesicles composed of synthetic lipids and measurement using total internal reflection fluorescence(TIRF) microscopy. Furthermore, we demonstrate applications including vesicle clustering mediated by proteins such as alpha-Synuclein(αSyn) and the influence of external ions by using TIRF microscopy. Moreover, we use this method to quantify the dependence of lipid composition and charge on vesicle clustering mediated by αSyn which is based on the methods previously reported.

关键词： Single molecule Alpha-Synuclein Lipids Vesicles Clustering Total internal reflection fluorescence microscopy

Super-resolution quantification of nanoscale damage to mitochondria in live cells

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Nano Research 2020年第8期13卷 2149-2155页

作者： Xintian Shao Qixin Chen Lianting Hu Zhiqi Tian Liuyi Liu Fei Liu Fengshan Wang Peixue Ling Zong-Wan Mao jiajie diao Shandong Academy of Pharmaceutical Sciences Key Laboratory of BiopharmaceuticalsEngineering Laboratory of Polysaccharide DrugsNational-Local Joint Engineering Laboratory of Polysaccharide DrugsJinan250101China Department of Cancer Biology University of Cincinnati College of MedicineCincinnatiOH45267USA School of Pharmaceutical Sciences Cheeloo College of MedicineShandong UniversityJinan250012China Xintian Shao Qixin Chenand Lianting Hu contributed equally to this work.Shandong First Medical University&Shandong Academy of Medical SciencesJinan250062China Xintian Shao Qixin Chenand Lianting Hu contributed equally to this work.School of Information ManagementWuhan UniversityWuhan430072China MOE Key Laboratory of Bioinorganic and Synthetic Chemistry School of ChemistrySun Yat-Sen UniversityGuangzhou510275China

Mitochondrial damage,characterized by altered morphological distribution and the damage of cristae,is closely associated with mitochondrial ***,imaging methods for capturing mitochondrial morphology at the nanoscale level in live samples remain unavailable,which seriously hinders the accurate evaluation and diagnosis of mitochondrial-related *** response,we propose a super-resolution quantification strategy based on structured illumination microscopy(SIM)for the rapid,accurate evaluation of mitochondrial *** the strategy,we accurately captured the morphological distribution of mitochondria at the nanoscale level in a way generally applicable to checking various cell processes and identifying patients with mitochondrial disease who exhibit the SLC25A46 *** also used algorithm-assisted super-resolution imaging to quantitatively analyze damage to mitochondrial cristae,which supports a novel drug screening strategy—high-resolution drug screening—for investigating drugs’pharmacodynamics on organelles in living *** short,our strategy improves the accurate examination of changes in mitochondrial morphology in living cells and indicates new ways in which SIM-imaging can assist in diagnosing mitochondrial disease at the single-cell level.

关键词： mitochondria structured illumination microscopy quantification analyze morphology cristae

Single-vesicle imaging quantifies calcium’s regulation of nanoscale vesicle clustering mediated by α-synuclein

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Microsystems & Nanoengineering 2020年第1期6卷 775-784页

作者： Bin Cai Jie Liu Yunfei Zhao Xiangyu Xu Bing Bu Dechang Li Lei Zhang Wei Dong Baohua Ji jiajie diao Department of Cancer Biology University of Cincinnati College of MedicineCincinnatiOH 45267USA Biomechanics and Biomaterials Laboratory Department of Applied MechanicsBeijing Institute of TechnologyBeijing 100081China Key Laboratory of Medical Electrophysiology of Ministry of Education and Medical Electrophysiological Key Laboratory of Sichuan Province Institute of Cardiovascular ResearchSouthwest Medical UniversityLuzhouSichuan 646000China Institute of Biomedical Engineering and Health Sciences Changzhou UniversityChangzhouJiangsu 213164China Institute of Applied Mechanics Department of Engineering MechanicsZhejiang UniversityHangzhou 310027China Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter(Ministry of Education) School of ScienceXi’an Jiaotong UniversityXi’an 710049China Beijing Advanced Innovation Center for Biomedical Engineering Beijing 100191China

Although numerous studies have shown that the proteinα-synuclein(α-Syn)plays a central role in Parkinson’s disease,dementia with Lewy bodies,and other neurodegenerative diseases,the protein’s physiological function remains poorly ***,despite recent reports suggesting that,under the influence of Ca^(2+),α-Syn can interact with synaptic vesicles,the mechanisms underlying that interaction are far from ***,we used single-vesicle imaging to quantify the extent to which Ca^(2+)regulates nanoscale vesicle clustering mediated by α-*** results revealed not only that vesicle clustering requiredα-Syn to bind to anionic lipid vesicles,but also that different concentrations of Ca^(2+)exerted different effects on howα-Syn induced vesicle *** particular,low concentrations of Ca^(2+)inhibited vesicle clustering by blocking the electrostatic interaction between the lipid membrane and the N terminus of α-Syn,whereas high concentrations promoted vesicle clustering,possibly due to the electrostatic interaction between Ca^(2+)and the negatively charged lipids that is independent of α-*** together,our results provide critical insights intoα-Syn’s physiological function,and how Ca^(2+) regulates vesicle clustering mediated by α-Syn.

关键词： inhibited insight promoted

Nanoscale monitoring of mitochondria and lysosome interactions for drug screening and discover

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Nano Research 2019年第5期12卷 1009-1015页

作者： Qixin Chen Xintian Shao Zhiqi Tian Yang Chen Payel Mondal Fei Liu Fengshan Wang Peixue Ling Weijiang He Kai Zhang Zijian Guo jiajie diao School of Pharmaceutical Sciences Shandong UniversityJinan 250101China Shandong Academy of Pharmaceutical Science Key Laboratory of BiopharmaceuticalsEngineering Laboratory of Polysaccharide DrugsNational-Local Joint Engineering Laboratory of Polysaccharide DrugsJinan 250101China Department of Cancer Biology University of Cincinnati College of MedicineCincinnatiOH 45267USA Department of Biochemistry University of Illinois at Urbana-ChampaignUrbanaIL 61801USA State Key Laboratory of Coordination Chemistry Coordination Chemistry InstituteSchool of Chemistry and Chemical EngineeringNanjing University Nanjing 210093China

Technology advances in genomics,proteomics,and metabolomics largely expanded the pool of potential therapeutic *** with the in vitro setting,cell-based screening assays have been playing a key role in the processes of drug discovery and *** the commonly used strategies based on colorimetric and cell viability,we reason that methods that capture the dynamic cellular events will facilitate optimal hit identification with high sensitivity and ***,we propose a live-cell screening strategy using structured illumination microscopy (SIM) combined with an automated cell colocalization analysis software,CellprofilerTM,to screen and discover drugs for mitochondria and lysosomes interaction at a nanoscale resolution in living *** strategy quantitatively benchmarks the mitochondria-lysosome interactions such as mitochondria and lysosomes contact (MLC) and *** automatic quantitative analysis also resolves fine changes of the mitochondria-lysosome interaction in response to genetic and pharmacological ***-resolution live-cell imaging on the basis of quantitative analysis opens up new avenues for drug screening and development by targeting dynamic organelle interactions at the nanoscale resolution,which could facilitate optimal hit identification and potentially shorten the cycle of drug discovery.

关键词： drug screening mitochondria lysosome mitophagy structured illumination microscopy

Heterogeneous oxidization of graphene nanosheets damages membrane

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Science China(Physics,Mechanics & Astronomy) 2019年第6期62卷 45-54页

作者： QianChun Wang XiaoBo Zhai Michael Crowe Lu Gou YinFeng Li DeChang Li Lei Zhang jiajie diao BaoHua Ji Biomechanics and Biomaterials Laboratory Department of Applied Mechanics Beijing Institute of Technology College of Science Xi'an University of Science and Technology Department of Cancer Biology University of Cincinnati College of Medicine MOE Key Laboratory for Nonequilibrium Synthesis and Modulation of Condensed Matter School of Science Xi'an Jiaotong University Department of Engineering Mechanics School of Naval Architecture Ocean and Civil Engineering and Key Laboratory of HydrodynamicsShanghai Jiao Tong University Institute of Applied Mechanics Department of Engineering Mechanics Zhejiang University

Graphene-based materials exhibit unique properties that have been sought to utilize for various potential applications. Many studies suggest that graphene-based materials can be cytotoxic, which may be attributed to destructive effects on cell ***, there still are conflicting results regarding interactions between graphene-based materials and lipid membranes. Here,through cryo-electron microscopy(Cryo-EM) and dye-leakage experiments along with in silico methods, we found that graphene oxide nanosheets induce significant membrane damage, while the effect of pristine graphene is negligible. We revealed the importance of heterogeneous oxidization of graphene-based nanosheets in damaging vesicle membranes. Moreover, that not only the oxidization degree but also the oxidization loci and membrane tension play important roles in the cytotoxicity of the graphene-based nanosheets.

关键词： graphene graphene oxide heterogeneous oxidization cytotoxicity lipid membrane

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A Novel Locomotion Rule Rmbedding Long Short-Term Memory Network with Attention for Human Locomotor Intent Classification Using Multi-Sensors Signals

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Computers, Materials & Continua 2024年第6期79卷 4349-4370页

作者： jiajie Shen Yan Wang Dongxu Zhang Key Laboratory of Symbol Computation and Knowledge Engineering Ministry of EducationColleague of Computer Science and TechnologyJilin UniversityChangchun130012China College of Software and Key Laboratory of Symbolic Computation and Knowledge Engineering of Ministry of EducationJilin UniversityChangchun130012China

Locomotor intent classification has become a research hotspot due to its importance to the development of assistive robotics and wearable *** work have achieved impressive performance in classifying steady locomotion ***,it remains challenging for these methods to attain high accuracy when facing transitions between steady locomotion *** to the similarities between the information of the transitions and their adjacent steady ***,most of these methods rely solely on data and overlook the objective laws between physical activities,resulting in lower accuracy,particularly when encountering complex locomotion modes such as *** address the existing deficiencies,we propose the locomotion rule embedding long short-term memory(LSTM)network with Attention(LREAL)for human locomotor intent classification,with a particular focus on transitions,using data from fewer sensors(two inertial measurement units and four goniometers).The LREAL network consists of two levels:One responsible for distinguishing between steady states and transitions,and the other for the accurate identification of locomotor *** classifier in these levels is composed of multiple-LSTM layers and an attention *** introduce real-world motion rules and apply constraints to the network,a prior knowledge was added to the network via a rule-modulating *** method was tested on the ENABL3S dataset,which contains continuous locomotion date for seven steady and twelve transitions *** results showed that the LREAL network could recognize locomotor intents with an average accuracy of 99.03%and 96.52%for the steady and transitions states,*** is worth noting that the LREAL network accuracy for transition-state recognition improved by 0.18%compared to other state-of-the-art network,while using data from fewer sensors.

关键词： Lower-limb prosthetics deep neural networks motion classification

Primitive functional groups directed distinct photocatalytic performance of imine-linked donor-acceptor covalent organic frameworks

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Nano Research 2024年第6期17卷 4994-5001页

作者： Xitong Ren jiajie Sun Yusen Li Feng Bai Key Laboratory for Special Functional Materials of Ministry of Education National and Local Joint Engineering Research Center for High-Efficiency Display and Lighting TechnologySchool of Materials Science and Engineeringand Collaborative Innovation Center of Nano Functional Materials and ApplicationsHenan UniversityKaifeng 475004China School of Physics and Electronics Henan UniversityKaifeng 475004China

The combination of donor-acceptor(D-A)structures presents a viable strategy for fabricating covalent organic frameworks(COFs)with exceptional photocatalytic ***,the selection of functional groups on donor or acceptor building blocks and their effect on the macroscopic properties of COFs are *** this study,we tactfully synthesized a pair of Py-DBT-COFs from the same pyrene(Py)donor and 4,7-diphenylbenzo[c][1,2,5]thiadiazole(DBT)acceptor cores with distinct primitive functional *** primitive functional groups of building units determine the photocatalytic properties of corresponding ***,Py-C-DBT-COF synthesized from Py-4CHO and DBT-2NH_(2)showcases a splendid H_(2)evolution rate as high as 21,377.7μmol/(g·h)(with 5 wt.%Pt)originating from better charge transfer capacity,which is significantly superior to that of Py-N-DBT-COF constructed from Py-4NH_(2)and *** distinct photocatalytic performances of the two COFs are demonstrated to originate from the different charge separation and transfer *** work supplies a new avenue for optimizing the photocatalytic performance of D-A COFs from the perspective of primitive functional group selections.

关键词： covalent organic frameworks functional group donor-acceptor photocatalysis

Nacre-inspired MXene-based film for highly sensitive piezoresistive sensing over a broad sensing range

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Bio-Design and Manufacturing 2024年第4期7卷 463-475页

作者： Gaofeng Wang Lingxian Meng Xinyi Ji Xuying Liu jiajie Liang Shuiren Liu School of Materials Science and Engineering Zhengzhou UniversityZhengzhou 450001China School of Materials Science and Engineering National Institute for Advanced MaterialsNankai UniversityTianjin 300350China

As the main component of wearable electronic equipment,flexible pressure sensors have attracted wide attention due to their excellent sensitivity and their promise with respect to applications in health monitoring,electronic skin,and human-computer ***,it remains a significant challenge to achieve epidermal sensing over a wide sensing range,with short response/recovery time and featuring seamless conformability to the skin *** is critical since the capture of minute electrophysiological signals is important for health care *** this paper,we report the preparation of a nacre-like MXene/sodium carboxymethyl cellulose(CMC)nanocomposite film with a“brick-and-mortar”interior structure using a vacuum-induced self-assembly *** synergistic behavior of the MXene“brick”and flexible CMC“mortar”contributes to attenuating interlamellar self-stacking and creates numerous variable conductive pathways on the sensing *** resulted in a high sensitivity over a broad pressure range(i.e.,0.03-22.37 kPa:162.13 kPa^(-1);22.37-135.71 kPa:127.88 kPa^(-1);135.71-286.49 kPa:100.58 kPa^(-1)).This sensor also has a low detection limit(0.85 Pa),short response/recovery time(8.58 ms/34.34 ms),and good stability(2000 cycles).Furthermore,we deployed pressure sensors to distinguish among tiny particles,various physiological signals of the human body,space arrays,robot motion monitoring,and other related applications to demonstrate their feasibility for a variety of health and motion monitoring use cases.

关键词： Flexible pressure sensor MXene Bioinspired Physiological signals Interlayer spacing

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