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Transport of intensity diffraction tomography with non-interferometric synthetic aperture for three-dimensional label-free microscopy

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light(Science & Applications) 2022年第10期11卷 2294-2307页

作者： jiaji li Ning Zhou Jiasong Sun Shun Zhou Zhidong Bai linpeng Lu Qian Chen Chao Zuo School of Electronic and Optical Engineering Nanjing University of Science and TechnologyNo.200 Xiaolingwei StreetNanjingJiangsu Province 210094China Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense Nanjing University of Science and TechnologyNanjingJiangsu Province 210094China Smart Computational Imaging Laboratory(SCILab) Nanjing University of Science and TechnologyNanjingJiangsu Province 210094China

We present a new label-free three-dimensional(3D)microscopy technique,termed transport of intensity diffraction tomography with non-interferometric synthetic aperture(TIDT-NSA).Without resorting to interferometric detection,TIDT-NSA retrieves the 3D refractive index(RI)distribution of biological specimens from 3D intensity-only measurements at various illumination angles,allowing incoherent-diffraction-limited quantitative 3D phase-contrast *** unique combination of z-scanning the sample with illumination angle diversity in TIDT-NSA provides strong defocus phase contrast and better optical sectioning capabilities suitable for high-resolution tomography of thick biological *** on an off-the-shelf bright-field microscope with a programmable light-emitting-diode(LED)illumination source,TIDT-NSA achieves an imaging resolution of 206 nm laterally and 520 nm axially with a high-NA oil immersion *** validate the 3D RI tomographic imaging performance on various unlabeled fixed and live samples,including human breast cancer cell lines MCF-7,human hepatocyte carcinoma cell lines HepG2,mouse macrophage cell lines RAW 264.7,Caenorhabditis elegans(***),and live Henrietta Lacks(HeLa)*** results establish TIDT-NSA as a new non-interferometric approach to optical diffraction tomography and 3D label-free microscopy,permitting quantitative characterization of cell morphology and time-dependent subcellular changes for widespread biological and medical applications.

关键词： illumination intensity synthetic

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A systematic review of the efficacy of traditional Chinese medicine retention enema in the treatment of ulcerative colitis

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Journal of Integrative Nursing 2024年第1期6卷 55-61页

作者： jiaji li ling TANG Ye li Jingjin XU Jing ZHANG School of Nursing Beijing University of Chinese MedicineBeijingChina Department of Nursing Dongfang Hospital of Beijing University of Chinese MedicineBeijingChina

The objective of this study is to summarize the efficacy and safety of traditional Chinese medicine(TCM)enema in the treatment of ulcerative colitis(UC).The randomized controlled trials on TCM enema intervention in the treatment of UC were searched in seven databases:PubMed,Embase,Web of Science,CBM,CNKI,Wanfang Database,and VIP Database from January 1,2013 to June 6,2022,and the data were analyzed using RevMan 5.3 software.A total of 18 studies involving 1514 UC patients were *** analysis results showed that compared with conventional Western medicine,Chinese medicine enema had a significant effect on UC,and the clinical effective rate of the experimental group using Chinese medicine enema was 4.45 times that of the control group using conventional Western medicine(odds ratio=4.45,95%confidence interval[3.27,6.06]).Therefore,Chinese medicine enema is effective in the treatment of UC,and can significantly reduce related symptoms.

关键词： Meta‑analysis retention enema systematic review traditional Chinese medicine ulcerative colitis

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Operating procedures of traditional Chinese medicine breast massage

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Journal of Integrative Nursing 2023年第2期5卷 151-154页

作者： Jingjin XU Hong CHEN Jing ZHANG jiaji li Ye li ling TANG School of Nursing Beijing University of Chinese MedicineBeijingChina Department of Galactophore Dongfang Hospital of Beijing University of Chinese MedicineBeijingChina Department of Nursing Dongfang Hospital of Beijing University of Chinese MedicineBeijingChina

Traditional Chinese medicine breast massage,also known as breast Tuina or manual expression of breast milk,is a technique of using certain manipulations to act on specific positions or acupoints of the breast to promote blood and lymphatic circulation of breast and facilitate the discharge of stagnant *** technique is widely used in China to treat breastfeeding problems such as breast engorgement,mastitis,and postpartum *** this article,we introduce the definition and standardization process of breast massage,elaborate the operating procedures of breast massage in detail,and summarize and discuss the current situation of its clinical application,so as to provide a reference for its wide application and in‑depth research.

关键词： Breast massage breast milk expression operating procedures traditional Chinese medicine Tuina

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Technical operation specification for pricking-cupping therapy

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Journal of Integrative Nursing 2023年第3期5卷 223-227页

作者： jiaji li Jingjin XU Jing ZHANG ling TANG Hong CHEN Ye li School of Nursing Beijing University of Chinese MedicineBeijingChina Department of Nursing Dongfang Hospital of Beijing University of Chinese MedicineBeijingChina Department of Galactophore Dongfang Hospital of Beijing University of Chinese MedicineBeijingChina

Pricking-cupping therapy is a Traditional Chinese Medicine(TCM)nursing technology that can adjust the viscera of the human body,make the meridians smooth,and Yin and Yang balanced mainly by means of relieving heat and detoxification,harmonizing Qi and blood,promoting blood circulation and removing blood stasis,dredging meridians and activating collaterals,reducing swelling and pain,purging heat and calming shock,clearing heat,and opening ***-cupping therapy has a long history and wide *** thousands of years of development,it has made great *** are widely used to treat lumbar disc herniation,herpes zoster,acute arthritis,migraine,and other diseases in *** the clinical practice and theoretical exploration of physicians of past dynasties,the therapeutic mechanism and application scope of pricking-cupping therapy have been greatly *** TCM practitioners have conducted in-depth researches on the operation norms of the therapy on the basis of the ancients,hoping to grasp the essence of the disease more accurately and make the rational use of the operation technology of the therapy.

关键词： Bloodletting puncture cupping external treatment of traditional Chinese medicine operating procedures pricking-cupping therapy Traditional Chinese Medicine nursing technology

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Resolution-enhanced intensity diffraction tomography in high numerical aperture label-free microscopy

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Photonics Research 2020年第12期8卷 1818-1826页

作者： jiaji li ALEX MATLOCK YUNZHE li QIAN CHEN LEI TIAN CHAO ZUO School of Electronic and Optical Engineering Nanjing University of Science and TechnologyNanjing 210094China Smart Computational Imaging Laboratory(SCILab) Nanjing University of Science and TechnologyNanjing 210094China Department of Electrical and Computer Engineering Boston UniversityBostonMassachusetts 02215USA

We propose label-free and motion-free resolution-enhanced intensity diffraction tomography(reIDT)recovering the 3D complex refractive index distribution of an *** combining an annular illumination strategy with a high numerical aperture(NA)condenser,we achieve near-diffraction-limited lateral resolution of 346 nm and axial resolution of 1.2μm over 130μm×130μm×8μm *** annular pattern matches the system’s maximum NA to reduce the data requirement to 48 intensity *** reIDT system is directly built on a standard commercial microscope with a simple LED array source and condenser lens adds-on,and promises broad applications for natural biological imaging with minimal hardware *** test the capabilities of our technique,we present the 3D complex refractive index reconstructions on an absorptive USAF resolution target and Henrietta Lacks(HeLa)and HT29 human cancer *** work provides an important step in intensity-based diffraction tomography toward high-resolution imaging applications.

关键词： intensity annular free

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High-speed in vitro intensity diffraction tomography

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Advanced Photonics 2019年第6期1卷 29-41页

作者： jiaji li Alex Matlock Yunzhe li Qian Chen Chao Zuo Lei Tian Nanjing University of Science and Technology School of Electronic and Optical EngineeringNanjingJiangsuChina Boston University Department of Electrical and Computer EngineeringBostonMassachusettsUnited States

We demonstrate a label-free,scan-free intensity diffraction tomography technique utilizing annular illumination(aIDT)to rapidly characterize large-volume three-dimensional(3-D)refractive index distributions in *** optimally matching the illumination geometry to the microscope pupil,our technique reduces the data requirement by 60 times to achieve high-speed 10-Hz volume *** eight intensity images,we recover volumes of∼350μm×100μm×20μm,with near diffraction-limited lateral resolution of∼487 nm and axial resolution of∼3.4μ*** attained large volume rate and high-resolution enable 3-D quantitative phase imaging of complex living biological samples across multiple length *** demonstrate aIDT’s capabilities on unicellular diatom microalgae,epithelial buccal cell clusters with native bacteria,and live Caenorhabditis elegans *** these samples,we recover macroscale cellular structures,subcellular organelles,and dynamic micro-organism tissues with minimal motion *** such features has significant utility in oncology,immunology,and cellular pathophysiology,where these morphological features are evaluated for changes in the presence of disease,parasites,and new drug ***,we simulate the aIDT system to highlight the accuracy and sensitivity of the proposed *** shows promise as a powerful high-speed,label-free computational microscopy approach for applications where natural imaging is required to evaluate environmental effects on a sample in real time.

关键词： computational microscopy three-dimensional imaging diffraction tomography phase retrieval.

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Multi-photon Up-conversion Luminescence of ErP_(5)O_(14) Noncrystalline

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Chinese Physics Letters 1993年第4期10卷 242-244页

作者： CHEN Xiaobo ZHANG Guangyin WANG Hong liU Yanbin li jiaji CHEN Jinkai ZHANG Wanlin SHANG Meiru Department of Physics Nankai UniversityTianjin 300071 Department of Physics Tianjin Education CollegeTianjin 300020 Research Institute of Artificial Crystalline Beijing 100018 Experiment Center Fujian Normal UniversityFuzhou 350007

In this paper we report the observation of multi-photon up-conversion luminescence phenomenon of ErP_(5)O_(14) noncrystalline induced by pulse DCM dye laser and clarify that the mechanism of up-conversion is the step ... 详细信息

关键词： photon luminescence

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Optical study on topological superconductor candidate Sr-doped Bi_(2)Se_(3)

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Chinese Physics B 2022年第11期31卷 536-541页

作者： Jialun liu Chennan Wang Tong lin liye Cao Lei Wang jiaji li Zhen Tao Nan Shen Rina Wu Aifang Fang Nanlin Wang Rongyan Chen Center for Advanced Quantum Studies and Department of Physics Beijing Normal UniversityBeijing 100875China International Center for Quantum Materials School of PhysicsPeking UniversityBeijing 100871China Collaborative Innovation Center of Quantum Matter Beijing 100871China

Utilizing infrared spectroscopy,we study the charge dynamics of the topological superconductor candidate Sr_(x)Bi_(2)Se_(3).The frequency-dependent reflectivity R(ω)demonstrates metallic feature and the scattering rate of the free carriers decreases with temperature *** plasma edge shows a slight blue shift upon cooling,similar to the behavior of Cu_(x)Bi_(2)Se_(3).As the carrier concentration n obtained by Hall resistivity increases slightly with the decreasing temperature,the effective mass is proved to increase as well,which is in contrast with that of Cu_(x)Bi_(2)Se_(3).We also perform the ultrafast pump-probe study on the Sr_(0.2)Bi_(2)Se_(3)*** its parent compound Bi_(2)Se_(3),three distinct relaxation processes are found to contribute to the transient ***,the deduced relaxation times are quite *** addition,the electron-optical-phonon coupling constant is identified to beλ=0.88.

关键词： topological superconductor infrared spectroscopy ultrafast spectroscopy

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Smart computational light microscopes(SCLMs)of smart computational imaging laboratory(SCILab)

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PhotoniX 2021年第1期2卷 105-169页

作者： Yao Fan jiaji li linpeng Lu Jiasong Sun Yan Hu Jialin Zhang Zhuoshi li Qian Shen Bowen Wang Runnan Zhang Qian Chen Chao Zuo School of Electronic and Optical Engineering Nanjing University of Science and TechnologyNo.200 Xiaolingwei Street210094 NanjingJiangsu ProvinceChina Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense Nanjing University of Science and TechnologyNo.200 Xiaolingwei Street210094 NanjingJiangsu ProvinceChina Smart Computational Imaging(SCI)Laboratory Nanjing University of Science and TechnologyNo.200 Xiaolingwei Street210094 NanjingJiangsu ProvinceChina Smart Computational Imaging Research Institute(SCRI)of Nanjing University of Science and Technology NanjingJiangsu Province 210019China.

Computational microscopy,as a subfield of computational imaging,combines optical manipulation and image algorithmic reconstruction to recover multi-dimensional microscopic images or information of *** recent years,the revolution in light-emitting diodes(LEDs),low-cost consumer image sensors,modern digital computers,and smartphones provide fertile opportunities for the rapid development of computational ***,diverse forms of computational microscopy have been invented,including digital holographic microscopy(DHM),transport of intensity equation(TIE),differential phase contrast(DPC)microscopy,lens-free on-chip holography,and Fourier ptychographic microscopy(FPM).These computational microscopy techniques not only provide high-resolution,label-free,quantitative phase imaging capability but also decipher new and advanced biomedical research and industrial ***,most computational microscopy techniques are still at an early stage of“proof of concept”or“proof of prototype”(based on commercially available microscope platforms).Translating those concepts to stand-alone optical instruments for practical use is an essential step for the promotion and adoption of computational microscopy by the wider bio-medicine,industry,and education *** this paper,we present four smart computational light microscopes(SCLMs)developed by our laboratory,i.e.,smart computational imaging laboratory(SCILab)of Nanjing University of Science and Technology(NJUST),*** microscopes are empowered by advanced computational microscopy techniques,including digital holography,TIE,DPC,lensless holography,and FPM,which not only enables multi-modal contrast-enhanced observations for unstained specimens,but also can recover their three-dimensional profiles *** introduce their basic principles,hardware configurations,reconstruction algorithms,and software design,quantify their imaging performance,and illustrate their typical applic

关键词： Microscope Quantitative phase imaging(QPI) Phase contrast Multi-contrast Operating software Biological applications

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Hybrid brightfield and darkfield transport of intensity approach for high-throughput quantitative phase microscopy

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Advanced Photonics 2022年第5期4卷 15-26页

作者： linpeng Lu jiaji li Yefeng Shu Jiasong Sun Jie Zhou Edmund Y.Lam Qian Chen Chao Zuo Nanjing University of Science and Technology Smart Computational Imaging Laboratory(SCILab)NanjingChina Nanjing University of Science and Technology School of Electronic and Optical EngineeringNanjingChina Jiangsu Key Laboratory of Spectral Imaging&Intelligent Sense NanjingChina Nanjing University of Science and Technology Smart Computational Imaging Research Institute(SCIRI)NanjingChina The University of Hong Kong Department of Electrical and Electronic EngineeringPokfulamHong KongChina

Transport of intensity equation(TIE)is a well-established non-interferometric phase retrieval approach that enables quantitative phase imaging(QPI)by simply measuring intensity images at multiple axially displaced *** advantage of a TIE-based QPI system is its compatibility with partially coherent illumination,which provides speckle-free imaging with resolution beyond the coherent diffraction ***,TIE is generally implemented with a brightfield(BF)configuration,and the maximum achievable imaging resolution is still limited to the incoherent diffraction limit(twice the coherent diffraction limit).It is desirable that TIE-related approaches can surpass this limit and achieve high-throughput[high-resolution and wide field of view(FOV)]*** propose a hybrid BF and darkfield transport of intensity(HBDTI)approach for highthroughput quantitative phase *** through-focus intensity stacks corresponding to BF and darkfield illuminations are acquired through a low-numerical-aperture(NA)objective *** high-resolution and large-FOV complex amplitude(both quantitative absorption and phase distributions)can then be synthesized based on an iterative phase retrieval algorithm taking the coherence model decomposition into *** effectiveness of the proposed method is experimentally verified by the retrieval of the USAF resolution target and different types of biological *** experimental results demonstrate that the half-width imaging resolution can be improved from 1230 nm to 488 nm with 2.5×expansion across a 4×FOV of 7.19 mm2,corresponding to a 6.25×increase in space-bandwidth product from∼5 to∼30.2 *** contrast to conventional TIE-based QPI methods where only BF illumination is used,the synthetic aperture process of HBDTI further incorporates darkfield illuminations to expand the accessible object frequency,thereby significantly extending the maximum available resolution from 2NA to∼5NA with a∼5×promotion of the coherent diffra

关键词： transport of intensity equation phase retrieval darkfield imaging high-throughput microscopy

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